LOCUS       sequence-286989-        1157 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1157)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3482
FEATURES             Location/Qualifiers
     source          1..1157
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(989..1013)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1021..1062
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgt acaacatgat ggagacggag
       61 ctgaagccgc cgggcccgca gcaaacttcg gggggcggcg gcggcaactc caccgcggcg
      121 gcggccggcg gcaaccagaa aaacagcccg gaccgcgtca agcggcccat gaatgccttc
      181 atggtgtggt cccgcgggca gcggcgcaag atggcccagg agaaccccaa gatgcacaac
      241 tcggagatca gcaagcgcct gggcgccgag tggaaacttt tgtcggagac ggagaagcgg
      301 ccgttcatcg acgaggctaa gcggctgcga gcgctgcaca tgaaggagca cccggattat
      361 aaataccggc cccggcggaa aaccaagacg ctcatgaaga aggataagta cacgctgccc
      421 ggcgggctgc tggcccccgg cggcaatagc atggcgagcg gggtcggggt gggcgccggc
      481 ctgggcgcgg gcgtgaacca gcgcatggac agttacgcgc acatgaacgg ctggagcaac
      541 ggcagctaca gcatgatgca ggaccagctg ggctacccgc agcacccggg cctcaatgcg
      601 cacggcgcag cgcagatgca gcccatgcac cgctacgacg tgagcgccct gcagtacaac
      661 tccatgacca gctcgcagac ctacatgaac ggctcgccca cctacagcat gtcctactcg
      721 cagcagggca cccctggcat ggctcttggc tccatgggtt cggtggtcaa gtccgaggcc
      781 agctccagcc cccctgtggt tacctcttcc tcccactcca gggcgccctg ccaggccggg
      841 gacctccggg acatgatcag catgtatctc cccggcgccg aggtgccgga acccgccgcc
      901 cccagcagac ttcacatgtc ccagcactac cagagcggcc cggtgcccgg cacggccatt
      961 aacggcacac tgcccctctc acacatgtgc ccaactttct tgtacaaagt ggttgatatc
     1021 ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgtagtaatg aactagtccg
     1081 taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgagctgtga
     1141 ttaggcttca caggatt
//
