LOCUS       sequence-287046-        1385 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1385)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1385)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3501
FEATURES             Location/Qualifiers
     source          1..1385
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1217..1241)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1249..1290
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcacca tggcagggat cttagcctgg
       61 ttctggaacg agaggttttg gctcccgcac aatgtcacct gggcggacct gaagaacacg
      121 gaggaggcca ccttcccgca ggctgaggac ctctatctcg cttttcccct ggccttctgt
      181 atcttcatgg tgcggctcat cttcgagaga tttgtagcca aaccgtgcgc catagccctc
      241 aacattcagg ccaatggacc acaaattgct ccgcccaatg ccattctgga aaaggtcttc
      301 actgcaatta caaagcatcc tgatgaaaag agattggaag gcctctccaa gcaactggac
      361 tgggatgttc gaagcattca gcgctggttt cgacaaagac gcaatcagga gaagccaagc
      421 acgctgacga ggttctgtga gagcatgtgg agattttcat tttaccttta tgtatttacc
      481 tacggagtca gattcctgaa aaagaccccc tggttgtgga atacgaggca ttgctggtac
      541 aactacccct atcagccact cacaactgac cttcactact attacatcct ggagctgtcg
      601 ttttattggt ctttgatgtt ttctcagttc actgatatca aaagaaagga ctttggcatt
      661 atgttcctgc accaccttgt atctattttc ttgattacct tttcatatgt caacaatatg
      721 gcccgagtag gaacgctggt cctttgtctt catgattcag ctgatgctct tctggaggct
      781 gccaaaatgg caaattatgc caagtttcag aaaatgtgtg atctcctgtt tgttatgttt
      841 gccgtggttt ttatcaccac acgactgggt atatttcctc tctgggtgtt aaataccaca
      901 ttatttgaaa gctgggagat cgttggacct tacccttcct ggtgggtttt taacctactg
      961 ctattgctag tacaagggtt gaactgcttc tggtcttact tgattgtgaa aatagcttgc
     1021 aaagctgttt caagaggcaa ggctgggaag tggaaccctt tacatgtgtc caaggatgat
     1081 cgaagtgata ttgagtctag ctcagatgag gaggactcag aacctccggg aaagaatccc
     1141 cacactgcga caaccaccaa tgggaccagt ggtaccaacg ggtatctcct gactggctcc
     1201 tgctccatgg atgatttgcc aactttcttg tacaaagtgg ttgatatcgg taagcctatc
     1261 cctaaccctc tcctcggtct cgattctacg tagtaatgaa ctagtccgta acttgaaagt
     1321 atttcgattt cttggcttta tatatcttgt ggaaaggacg atgaagacct ggacacggga
     1381 tgatg
//
