LOCUS       sequence-287058-        1412 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1412)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1412)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3505
FEATURES             Location/Qualifiers
     source          1..1412
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1244..1268)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1276..1317
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatga atatccatat gaagagaaaa
       61 acaataaaga atatcaacac ctttgagaac agaatgttaa tgcttgatgg gatgccggca
      121 gtcagagtca aaacagagct tttggaatct gaacaagggt ctccaaacgt ccacaactat
      181 cccgatatgg aagccgttcc cctgttgcta aataatgtga aaggggagcc cccggaggac
      241 tcgttatctg tagatcactt ccaaacacaa actgagccag tggacttgtc aataaacaaa
      301 gccaggacgt cccctactgc cgtttcatcc tccccagttt ccatgacagc atctgcctcc
      361 tcaccttctt caacttcaac ctcttcatcg tcttctagtc gtctagcctc atccccaact
      421 gttatcacat cagtatcttc agcgtcatct tcgtcaacag tattaactcc agggcccctt
      481 gtggcctctg catctggtgt tggaggccag cagtttttgc acattatcca tcccgtaccg
      541 ccttcaagtc ccatgaattt acagtctaac aaactgagtc atgttcaccg catccccgtg
      601 gtggtacagt cggtgcctgt tgtctacaca gctgtaaggt cacctggaaa tgtgaacaac
      661 actattgtcg tgccgctttt ggaggatggg agaggccatg gcaaagcaca aatggacccc
      721 cgaggcctat ctcccagaca aagtaaaagt gacagtgatg atgatgacct gccaaatgtg
      781 accttagata gcgttaatga aactggatct acggcccttt ccatagccag agcagtacaa
      841 gaggtacatc cgtccccagt atcaagggtc cggggaaatc gaatgaataa tcaaaagttt
      901 ccttgttcaa tttcaccatt tagtattgag agcacaagac gccagagacg gtctgaatcc
      961 ccagactcca gaaaacggcg tatccacaga tgtgattttg agggatgcaa caaagtgtac
     1021 acaaaaagtt ctcacctgaa ggctcaccgg aggacacata caggagagaa accttacaag
     1081 tgtacctggg aaggctgcac ctggaagttc gctcgttcag atgaactgac gaggcattac
     1141 cgcaaacata cgggagtgaa gccattcaag tgcgcggact gtgatcgcag cttttcccgg
     1201 tcagatcatt tggccctgca ccgccggagg catatgttgg tgtgcccaac tttcttgtac
     1261 aaagtggttg atatcggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag
     1321 taatgaacta gtccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga
     1381 aaggacgaga aattcaatct agggcccatt tg
//
