LOCUS       sequence-287115-        1508 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1508)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3524
FEATURES             Location/Qualifiers
     source          1..1508
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1340..1364)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1372..1413
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgc cgaaccgtaa ggccagccgg
       61 aatgcttact atttcttcgt gcaggagaag atccccgaac tacggcgacg aggcctgcct
      121 gtggctcgcg ttgctgatgc catcccttac tgctcctcag actgggcgct tctgagggag
      181 gaagaaaagg agaaatacgc agaaatggct cgagaatgga gggccgctca gggaaaggac
      241 cctgggccct cagagaagca gaaacctgtt ttcacaccac tgaggaggcc aggcatgctt
      301 gtaccaaagc agaatgtttc acctccagat atgtcagctt tgtctttaaa aggtgatcaa
      361 gctctccttg gaggcatttt ttattttttg aacattttta gccatggcga gctacctcct
      421 cattgtgaac agcgcttcct cccttgtgaa attggctgtg ttaagtattc tctccaagaa
      481 ggtattatgg cagatttcca cagttttata aatcctggtg aaattccacg aggatttcga
      541 tttcattgtc aggctgcaag tgattctagt cacaagattc ctatttcaaa ttttgaacgt
      601 gggcataacc aagcaactgt gttacaaaac ctttatagat ttattcatcc caacccaggg
      661 aactggccac ctatctactg caagtctgat gatagaacca gagtcaactg gtgtttgaag
      721 catatggcaa aggcatcaga aatcaggcaa gatctacaac ttctcactgt agaggacctt
      781 gtagtgggga tctaccaaca aaaatttctc aaggagccct ctaagacttg gattcgaagc
      841 ctcctagatg tggccatgtg ggattattct agcaacacaa ggtgcaagtg gcatgaagaa
      901 aatgatattc tcttctgtgc tttagctgtt tgcaagaaga ttgcgtactg catcagtaat
      961 tctctggcca ctctctttgg aatccagctc acagaggctc atgtaccact acaagattat
     1021 gaggccagca atagtgtgac acccaaaatg gttgtattgg atgcagggcg ttaccagaag
     1081 ctaagggttg ggagttcagg attctctcat ttcaactctt ctaatgagga acaaagatca
     1141 aacacaccca ttggtgacta cccatctagg gcaaaaattt ctggccaaaa cagcagcgtt
     1201 cggggaagag gaattacccg cttactagag agcatttcca attcttccag caatatccac
     1261 aaattctcca actgtgacac ttcactctca ccttacatgt cccaaaaaga tggatacaaa
     1321 tctttctctt ccttatctta cccaactttc ttgtacaaag tggttgatat cggtaagcct
     1381 atccctaacc ctctcctcgg tctcgattct acgtagtaat gaactagtcc gtaacttgaa
     1441 agtatttcga tttcttggct ttatatatct tgtggaaagg acgacagacc acattccgaa
     1501 gattatta
//
