LOCUS       sequence-287124-        1850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3527
FEATURES             Location/Qualifiers
     source          1..1850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1682..1706)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1714..1755
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatga agaccccggc ggacacaggg
       61 tttgccttcc cggattgggc ctacaagcca gagtcgtccc ctggctcaag gcagatccag
      121 ctgtggcact ttatcctgga gctgctgcgg aaggaggagt accagggcgt cattgcctgg
      181 cagggggact acggggaatt cgtcatcaaa gaccctgatg aggtggcccg gctgtggggc
      241 gttcgcaagt gcaagcccca gatgaattac gacaagctga gccgggccct gcgctattac
      301 tataacaagc gcattctgca caagaccaag gggaaacggt tcacctacaa gttcaatttc
      361 aacaaactgg tgctggtcaa ttacccattc attgatgtgg ggttggctgg gggtgcagtg
      421 ccccagagtg ccccgccagt gccgtcgggt ggtagccact tccgcttccc tccctcaacg
      481 ccctccgagg tgctgtcccc caccgaggac ccccgctcac caccagcctg ctcttcatct
      541 tcatcttccc tcttctcggc tgtggtggcc cgccgcctgg gccgaggctc agtcagtgac
      601 tgtagtgatg gcacgtcaga gctggaggaa ccgctgggag aggatccccg cgcccgacca
      661 cccggccctc cggatctggg tgccttccga gggcccccgc tggcccgcct gccccatgac
      721 cctggtgtct tccgagtcta tccccggcct cggggtggcc ctgaacccct cagccccttc
      781 cctgtgtcgc ctctggccgg tcctggatcc ctgctgcccc ctcagctctc cccggctctg
      841 cccatgacgc ccacccacct ggcctacact ccctcgccca cgctgagccc gatgtacccc
      901 agtggtggcg gggggcccag cggctcaggg ggaggctccc acttctcctt cagccctgag
      961 gacatgaaac ggtacctgca ggcccacacc caaagcgtct acaactacca cctcagcccc
     1021 cgcgccttcc tgcactaccc tgggctggtg gtgccccagc cccagcgccc tgacaagtgc
     1081 ccgctgccgc ccatggcacc cgagacccca ccggtcccct cctcggcctc gtcatcctct
     1141 tcttcttctt cctccccatt caagtttaag ctccagccgc ccccactcgg acgccggcag
     1201 cgggcagctg gggagaaggc cgtagccggt gctgacaaga gcggtggcag tgcaggcggg
     1261 ctggctgagg gggcaggggc gctagcccca ccgcccccgc caccacagat caaggtggag
     1321 cccatctcgg aaggcgagtc ggaggaggta gaggtgactg acatcagtga tgaggatgag
     1381 gaagacgggg aggtgttcaa gacgccccgt gccccacctg caccccctaa gcctgagccc
     1441 ggcgaggcac ccggggcatc ccagtgcatg cccctcaagc tacgctttaa gcggcgctgg
     1501 agtgaagact gtcgcctcga agggggtggg ggccccgctg ggggctttga ggatgagggt
     1561 gaggacaaga aggtgcgtgg ggaggggcct ggggaggctg gggggcccct caccccaagg
     1621 cgggtgagct ctgacctcca gcatgccacg gcccagctct ccctggagca ccgagactcc
     1681 tgcccaactt tcttgtacaa agtggttgat atcggtaagc ctatccctaa ccctctcctc
     1741 ggtctcgatt ctacgtagta atgaactagt ccgtaacttg aaagtatttc gatttcttgg
     1801 ctttatatat cttgtggaaa ggacgaccag tcctcggcgc acgaataggt
//
