LOCUS       sequence-287184-         752 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 752)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF3547
FEATURES             Location/Qualifiers
     source          1..752
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    13..37
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(584..608)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             616..657
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 atcgattgat caacaagttt gtacaaaaaa gttggcatgc cgcgcgtcgt gcccgaccag
       61 agaagcaagt tcgagaacga ggagtttttt aggaagctga gccgcgagtg tgagattaag
      121 tacacgggct tcagggaccg gccccacgag gaacgccagg cacgcttcca gaacgcctgc
      181 cgcgacggcc gctcggaaat cgcttttgtg gccacaggaa ccaatctgtc tctccagttt
      241 tttccggcca gctggcaggg agaacagcga caaacaccta gccgagagta tgtcgactta
      301 gaaagagaag caggcaaggt atatttgaag gctcccatga ttctgaatgg agtctgtgtt
      361 atctggaaag gctggattga tctccaaaga ctggatggta tgggctgtct ggagtttgat
      421 gaggagcgag cccagcagga ggatgcatta gcacaacagg cctttgaaga ggctcggaga
      481 aggacacgcg aatttgaaga tagagacagg tctcatcggg aggaaatgga ggtgagagtt
      541 tcacagctgc tggcagtaac tggcaagaag acaacaagac cctacccaac tttcttgtac
      601 aaagtggttg atatcggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag
      661 taatgaacta gtccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga
      721 aaggacgaac ccgatcctgg gattccctat cg
//
