LOCUS       sequence-288030-        6182 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  FKBP12 only.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6182)
  AUTHORS   Varnai P, Thyagarajan B, Rohacs T, Balla T
  TITLE     Rapidly inducible changes in phosphatidylinositol 4,5-bisphosphate 
            levels influence multiple regulatory functions of the lipid in 
            intact living cells.
  JOURNAL   J Cell Biol. 2006 Nov 6;175(3):377-82.
  PUBMED    17088424
REFERENCE   2  (bases 1 to 6182)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2006-11-6"; volume: "175(3)"; pages: "377-82"
COMMENT     Sequence Label: mRFP-FKBP12
FEATURES             Location/Qualifiers
     source          1..6182
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        21..212
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     169..189
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        complement(214..318)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      345..800
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(432..451)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     642..663
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    807..928
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(818..837)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     872..891
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(join(2227..2550,2551..2553))
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKLE"
     CDS             complement(2611..3285)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             complement(2611..3285)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(2670..2693)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(2868..2887)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     3137..3155
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      3282..3291
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(3330..3533)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(3359..3379)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        3534..3837
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(4012..4600)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4092..4111)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    4929..4976
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     5001..5020
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(5208..6002)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(5208..6002)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5319..5338)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     5929..5948
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(join(6037..6182,1..212))
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      join(6051..6182,1..4)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 ggatgggcgg agttaggggc gggactatgg ttgctgacta attgagatgc atgctttgca
       61 tacttctgcc tgctggggag cctggggact ttccacacct ggttgctgac taattgagat
      121 gcatgctttg catacttctg cctgctgggg agcctgggga ctttccacac cctaactgac
      181 acacattcca cagctggttc tttccgcctc aggactcttc ctttttcaat attattgaag
      241 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
      301 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgcgc cctgtagcgg
      361 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
      421 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
      481 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
      541 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
      601 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
      661 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
      721 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
      781 aatattaacg cttacaattt acgcgttaag atacattgat gagtttggac aaaccacaac
      841 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
      901 aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca
      961 ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat
     1021 ggctgattat gatcagttat ctagatccgg tggatcccgg gcccgcggta cctagagaat
     1081 tcgattattg tggagcaggg cagtgatagt gtcttcagcc aacacatcat agccaatctg
     1141 cttctgcatg aagccaaact gcttggctat atactcctgg ttcttcctgt agtcttgctg
     1201 cgagtgtctc agcaccctgt agcagagccg gcagatgtgt ctgaaaggag cgtgccgctg
     1261 gtccccgagc tcttccagcc gaagcattgg gccatcaccg cagtctgtga atggggcttg
     1321 taacaacttg aagatctgct tgagaatatt ctgttctctc atcagtttct gccgttctct
     1381 gttgggcttg gagaagacaa cttcgagaac atcttgacca gaattagttc caccagtgac
     1441 gaagtaaacc aaatcttcta gcagcttggt tacagacctc ctttcattct gggtgatggt
     1501 gcccttctct agcttcccag caatggagcc cagcaccttg ctggcatcat tggcaaagtc
     1561 caggtcccga acttcagcag gagaaaccgg aactatggca aatgcttcct tatcctcctt
     1621 cacaggagag gtgccaattt tcagcatcac gggcttttct tcttccttgt caataggaat
     1681 atttgtgctg tgaacccagg tattagtaca taggtgtctg agccgaacat aagagttcct
     1741 tgggacaagg ctgtcacctc cacgcagagt ggtgggatct agctcgaaaa tggaggagat
     1801 gtcattgcct tcaggcacag agaccaggga gtataccatc ttttcttggg cattccgcaa
     1861 cctacttcga gaggcgtcct gatcagggtc cactgagggc tgaaactcca ggcattcttc
     1921 ctcaaagtca gggtctacct ctgctgccaa gtaatgcccc gtggccagat gcttgaaacg
     1981 gaaaaggctg ttccaatacc ctgctccgcc ccgacatggg tcatgctgga ccacctccac
     2041 ctcccacagg gcttttgaac tggtggcaga tgtggccgac tgccggcccg tggttctcag
     2101 gaagacgtgc tgcttcttcc tgtgttcgtc acaggtgaga aacttctcct gctcagcatg
     2161 aaacagcctc accacgtcac ccccctttaa tatgtcgtct ttgttatcac tccatttcac
     2221 tcgagctcat tccagtttta gaagctccac atcgaagacg agagtggcat gtggtgggat
     2281 gatgcctggg tgcccagtgg caccataggc ataatctgga gatatagtca gtttggctct
     2341 ctgacccaca ctcatctggg caaccccttc ttcccagcct cggatcacct cctgcttgcc
     2401 tagcataaac ttaaagggct tgtttctgtc ccgggaggaa tcaaatttct ttccatcttc
     2461 aagcatcccg gtgtagtgca ccacgcaggt ctggccgcgc ttggggaagg tgcgcccgtc
     2521 tcctggggag atggtttcca cctgcactcc catcgccgct cgagctgctc ctcctgctcc
     2581 cgcggccgcg cttcgagatc tgagtccgga ggcgccggtg gagtggcggc cctcggcgcg
     2641 ctcgtactgt tccacgatgg tgtagtcctc gttgtgggag gtgatgtcca gcttgatgtc
     2701 ggtcttgtag gcgccgggca gctgcacggg cttcttggcc atgtaggtgg tcttgacctc
     2761 ggcgtcgtag tggccgccgt ccttcagctt cagcctcatc ttgatctcgc ccttcagggc
     2821 gccgtcctcg gggtacatcc gctcggtgga ggcctcccag cccatggtct tcttctgcat
     2881 tacggggccg tcggagggga agttggtgcc gcgcagcttc accttgtaga tgaactcgcc
     2941 gtcctgcagg gaggagtcct gggtcacggt caccacgccg ccgtcctcga agttcatcac
     3001 gcgctcccac ttgaagccct cggggaagga cagcttcaag tagtcgggga tgtcggcggg
     3061 gtgcttcacg taggccttgg agccgtactg gaactgaggg gacaggatgt cccaggcgaa
     3121 gggcaggggg ccgcccttgg tcaccttcag cttggcggtc tgggtgccct cgtaggggcg
     3181 gccctcgccc tcgccctcga tctcgaactc gtggccgttc acggagccct ccatgcgcac
     3241 cttgaagcgc atgaactcct tgatgacgtc ctcggaggag gccatggtgg cgaccggtag
     3301 cgctagcgga tctgacggtt cactaaacca gctctgctta tatagacctc ccaccgtaca
     3361 cgcctaccgc ccatttgcgt caatggggcg gagttgttac gacattttgg aaagtcccgt
     3421 tgattttggt gccaaaacaa actcccattg acgtcaatgg ggtggagact tggaaatccc
     3481 cgtgagtcaa accgctatcc acgcccattg atgtactgcc aaaaccgcat caccatggta
     3541 atagcgatga ctaatacgta gatgtactgc caagtaggaa agtcccataa ggtcatgtac
     3601 tgggcataat gccaggcggg ccatttaccg tcattgacgt caataggggg cgtacttggc
     3661 atatgataca cttgatgtac tgccaagtgg gcagtttacc gtaaatactc cacccattga
     3721 cgtcaatgga aagtccctat tggcgttact atgggaacat acgtcattat tgacgtcaat
     3781 gggcgggggt cgttgggcgg tcagccaggc gggccattta ccgtaagtta tgtaacgcgg
     3841 aactccatat atgggctatg aactaatgac cccgtaattg attactatta ataactaatg
     3901 catggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     3961 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     4021 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     4081 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     4141 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     4201 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     4261 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     4321 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     4381 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     4441 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     4501 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     4561 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     4621 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     4681 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     4741 aagtatatat gagtaacctg aggctatggc agggcctgcc gccccgacgt tggctgcgag
     4801 ccctgggcct tcacccgaac ttggggggtg gggtggggaa aaggaagaaa cgcgggcgta
     4861 ttggccccaa tggggtctcg gtggggtatc gacagagtgc cagccctggg accgaacccc
     4921 gcgtttatga acaaacgacc caacaccgtg cgttttattc tgtcttttta ttgccgtcat
     4981 agcgcgggtt ccttccggta ttgtctcctt ccgtgtttca gttagcctcc ccctagggtg
     5041 ggcgaagaac tccagcatga gatccccgcg ctggaggatc atccagccgg cgtcccggaa
     5101 aacgattccg aagcccaacc tttcatagaa ggcggcggtg gaatcgaaat ctcgtgatgg
     5161 caggttgggc gtcgcttggt cggtcatttc gaaccccaga gtcccgctca gaagaactcg
     5221 tcaagaaggc gatagaaggc gatgcgctgc gaatcgggag cggcgatacc gtaaagcacg
     5281 aggaagcggt cagcccattc gccgccaagc tcttcagcaa tatcacgggt agccaacgct
     5341 atgtcctgat agcggtccgc cacacccagc cggccacagt cgatgaatcc agaaaagcgg
     5401 ccattttcca ccatgatatt cggcaagcag gcatcgccat gggtcacgac gagatcctcg
     5461 ccgtcgggca tgctcgcctt gagcctggcg aacagttcgg ctggcgcgag cccctgatgc
     5521 tcttcgtcca gatcatcctg atcgacaaga ccggcttcca tccgagtacg tgctcgctcg
     5581 atgcgatgtt tcgcttggtg gtcgaatggg caggtagccg gatcaagcgt atgcagccgc
     5641 cgcattgcat cagccatgat ggatactttc tcggcaggag caaggtgaga tgacaggaga
     5701 tcctgccccg gcacttcgcc caatagcagc cagtcccttc ccgcttcagt gacaacgtcg
     5761 agcacagctg cgcaaggaac gcccgtcgtg gccagccacg atagccgcgc tgcctcgtct
     5821 tgcagttcat tcagggcacc ggacaggtcg gtcttgacaa aaagaaccgg gcgcccctgc
     5881 gctgacagcc ggaacacggc ggcatcagag cagccgattg tctgttgtgc ccagtcatag
     5941 ccgaatagcc tctccaccca agcggccgga gaacctgcgt gcaatccatc ttgttcaatc
     6001 atgcgaaacg atcctcatcc tgtctcttga tcgatctttg caaaagccta ggcctccaaa
     6061 aaagcctcct cactacttct ggaatagctc agaggccgag gcggcctcgg cctctgcata
     6121 aataaaaaaa attagtcagc catggggcgg agaatgggcg gaactgggcg gagttagggg
     6181 cg
//
