LOCUS       sequence-288035-       10403 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10403)
  AUTHORS   Dorn KV, Hughes CE, Rohatgi R
  TITLE     A Smoothened-Evc2 Complex Transduces the Hedgehog Signal at Primary 
            Cilia.
  JOURNAL   Dev Cell. 2012 Sep 12. pii: S1534-5807(12)00323-1. doi: 
            10.1016/j.devcel.2012.07.004.
  PUBMED    22981989
REFERENCE   2  (bases 1 to 10403)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Cell. 
            2012 Sep 12. pii: S1534-5807(12)00323-1. doi: 
            10.1016/j.devcel.2012.07.004."
COMMENT     Sequence Label: pEF5/FRT-DEST EVC2-TEV-YFP-Flag
FEATURES             Location/Qualifiers
     source          1..10403
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        143..1321
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          374..1312
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1269..1289
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     1338..1357
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1338..1356
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1435..1459
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5179..5199
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             join(5203..5205,5206..5208,5209..5919)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5248..5269)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5509..5528)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5856..5877
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5950..5973
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    complement(5991..6015)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6068..6109
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(6147..6164)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6153..6377
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6510..6529)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    6661..6708
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             6716..7738
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     CDS             6716..7738
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    7868..7989
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7905..7924)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7959..7978
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8038..8054)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8038..8054)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8051..8073)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8062..8078
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8086..8092,8093..8110,8111..8116))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8131..8152
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8269..8286)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8440..9028)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8520..8539)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9199..9990,9991..10059))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9822..9841
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10060..10164)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10239..10258)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cgatgtacgg gccagatata cgcgttgaca ttgattattg actaggcttt tgcaaaaagc
       61 tttgcaaaga tggataaagt tttaaacaga gaggaatctt tgcagctaat ggaccttcta
      121 ggtcttgaaa ggagtgggaa ttggctccgg tgcccgtcag tgggcagagc gcacatcgcc
      181 cacagtcccc gagaagttgg ggggaggggt cggcaattga accggtgcct agagaaggtg
      241 gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg
      301 gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc
      361 cgccagaaca caggtaagtg ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta
      421 tggcccttgc gtgccttgaa ttacttccac ctggctgcag tacgtgattc ttgatcccga
      481 gcttcgggtt ggaagtgggt gggagagttc gaggccttgc gcttaaggag ccccttcgcc
      541 tcgtgcttga gttgaggcct ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca
      601 ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc atttaaaatt tttgatgacc
      661 tgctgcgacg ctttttttct ggcaagatag tcttgtaaat gcgggccaag atctgcacac
      721 tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg
      781 ttcggcgagg cggggcctgc gagcgcggcc accgagaatc ggacgggggt agtctcaagc
      841 tggccggcct gctctggtgc ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc
      901 aaggctggcc cggtcggcac cagttgcgtg agcggaaaga tggccgcttc ccggccctgc
      961 tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag cgggcgggtg agtcacccac
     1021 acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg
     1081 ggcgccgtcc aggcacctcg attagttctc gagcttttgg agtacgtcgt ctttaggttg
     1141 gggggagggg ttttatgcga tggagtttcc ccacactgag tgggtggaga ctgaagttag
     1201 gccagcttgg cacttgatgt aattctcctt ggaatttgcc ctttttgagt ttggatcttg
     1261 gttcattctc aagcctcaga cagtggttca aagttttttt cttccatttc aggtgtcgtg
     1321 aggaattagc ttggtactaa tacgactcac tatagggaga cccaagctgg ctaggtaagc
     1381 ttggtaccga gctcggatcc actagtccag tgtggtggaa ttctgcagat atcaacaagt
     1441 ttgtacaaaa aagcaggctg gcgccggaac caattcagtc gactggatcc ggtaccgaat
     1501 tccaccatgg gggcgacggg ccccacgggc gctgggggac gagccacgtg ggtgctggct
     1561 gggaatatcc tggccgctgc cctggtactg ggctcgggtc cgcgtgcact tccaccatcc
     1621 ttccctgccc tgggcccagg gtcaccatct cggcccggcc cagcaggacc ctgggcaagc
     1681 tcgcagtaca gcgatatttc aagagaagcc cgaggtccct ttgagaatgg agtgatattt
     1741 caaaaatgct cactggtgtc tgggcagagc gaatcgcaga ccatgcacgt gcagctgtcc
     1801 gtgaacaaca ccaggacgcc cacatcagtc aacctttcga acctgctcgt gctagatgag
     1861 atcactggcc ttgctgtgaa ggagtcgcct ggaaataata ctcaggatgg aatccagact
     1921 ttcagaaaga gctttctgca agtgggagaa tgctactctg tcagctacac agcctcgctg
     1981 gaccccacag cccttgggac tggggagagc ctggatcttc ctgcccggct catctttcag
     2041 agcccctcac agaacagaac tcagctaaaa gcccccttta ccatcacagt ggaagaaaag
     2101 ataatggttc ttcctaacca tggtcttcac gcagcaggct tcatcgcagc cttcctcatc
     2161 tccctcctcc tgaccgtggc agccctgttc ttcttggcga ggggtcgatg tctgcaggga
     2221 ggcatgctgt ccagatgccg gattcagcat cctgagaaca agctggagcc ctcgcccttc
     2281 acctctgcta acggtgtgag ccaggacctt tccctcaatg accaagtggt tgccatcctg
     2341 acttctgagg agcctgggag catgcttcaa gccctagaag agttggagat cgcaaccctg
     2401 aatcaggcag atgcggatct ggaggcttgt cgaaaccaga ttagtaagga catcattgcc
     2461 cttttgatga agaatctggt cagcggtggt cacctctctc cgcaaacaga aaggaagatg
     2521 gccgctgctt tcaaaaagca gtttctgctg ttggaaaatg aaatacagga ggagtatgag
     2581 cggaagatgt tggcactgac ggcagagtgt gacctagaga tgaggaagaa gacagaaaac
     2641 cagtaccaga gggagatggt ggccatggag gaagcagaag aggtgttgaa gagagttagc
     2701 gagaggtctg ctgccgagtg cagcagcctt ttgcggaccc tccatgggct ggagcaggag
     2761 gacatgcaga ggtcccttac tctggaccaa gccgaggact ttgctcaagc acacaggcag
     2821 ctggctgttt tccagaggaa tgaattacac agcatcgtct atacccagat acagagtgct
     2881 gtctccaaag gggaactgag gcctgaggtg gctaaaatga tgctgcaaga ctactctaaa
     2941 acacaggaga gtgtagaaga gctgatggac ttcttccagg ccaccaagcg ataccatctc
     3001 agcaagaggt ttggccacag agagtacctg gtccagagac tgcaggccat ggagacccgg
     3061 gtgcaagggc tgctgaacac cgcggctacc cagctaacaa gcctcatcca taaacatgag
     3121 agggctggct acttggatga agatcagatg gaaacgctat tggagcgtgc acagacagaa
     3181 accttttcta taaaacagaa gctggacaat gacttaaagc aggaaaagaa aaggctccac
     3241 cagagattaa taaccaggag gcggcgagag cttctgcaga agcacaagga gcagcagaag
     3301 gagcaggtgt cccttggcga ggcctccagc accgcggagg acgcagtcca gtacctgcac
     3361 cagtggagga gcgtgatggc cgagcacacc gctgccctgg aggagctgca ggaacgtctg
     3421 gaccaggctg ccctggatga cctccgggtc ctgactgtgt cgctgtcgga gaaagccacg
     3481 gaagagctga ggcgcctgca gagcacggcc atgacacagg agctgctgaa gcgcagtgct
     3541 ccctggctct tcctgcagca gatcttagag gagcacagcc gggagtctgc ggctcgcacc
     3601 acgcaactgg aggcagagga gagggaacgt ggccaggagc tggtgcaggg cgtgaggcag
     3661 aggctacagc aggatgcact ggaggcctac acggaagagc aggcagagct gaggcactgg
     3721 gagcacttgg tgttcatgaa actctgctgt gcggccatct cattgtctga agaagacctg
     3781 ctccgggtaa ggcaggaggc acagggctgc ttcagccagc tggaccggag cttggccctc
     3841 cccagagtcc gtgcacgagt gttgcagcag caggcgcaga tggcctggcg ggaagcagag
     3901 tttcggaaac tggaccaggc cctggctgcg cctgagctgc agtccaaggc aagaaagctg
     3961 cgttccaagg gcagaggcaa ggccgatctt ctgaagaaga acctggagga taagatccga
     4021 ctattcgagg agcgggcccc tgtagaactg gctgaccagg tgcgagggga attgctccaa
     4081 gagagggtcc agaggctgga ggcccaggag gcgcactttg ctgagtcact tgtggctctg
     4141 cagttccaga aggtggcccg ggctgctgag accctctcag tctacaccgc cctgctcagc
     4201 attcaagacc tgctgctggg ggagctgagt gagtcggaga ccctgaccaa gtcagcctgt
     4261 gtgcagatcc tggagtccca caggccggag ctgcaggagc tgcaggagct ggagaggaag
     4321 ctggaggacc agctggtgca gcaggaagag gccgagcagc agcgggtcct ggagagctgg
     4381 cagcggtggg cggccgatgg acctgggctg agcgaacctg aggagatgga tcctgaaagg
     4441 caggtctctg ccatcctgcg gcaagccctg aacaagggcc agaagctgct ggagcagcat
     4501 cagcagaggg tgagagagga gtggcagaac ggtgcggtgc tggaagattc tctggaaagc
     4561 attgaagctg acaccatggc cagcctttgc agccagggac tgaggctggt gtcatacctc
     4621 tcaaggatga caatggtacc aggcagcaca ctactgcgac tcctgagtgt ggtgcttcca
     4681 gcggcttcac agcctcagct actggccctg ctggacgcag tcagtgagaa gcactccgac
     4741 cacacagctg agaatgagag cagtggtgag caggctcagg cagagcagag caagaggagg
     4801 aaacaccaag tttggtggaa ggttttggac agcagattca gagcagatct ggtgagtcaa
     4861 ggactggaga ggatgctctg ggcccgccag aagaaggagc gcatattgaa gaagatatat
     4921 gttcccgtcc aggagagggt gatgtttcct gggaaaggaa gctggccaca cctgtcccta
     4981 gaacccattg gagaactggc tcccatcccc atcactgggg cagacgctat ggacatatta
     5041 aacacgggag aaaagatctt tgtattcaga agcccaaggg agcccgagat ctctttgcgt
     5101 gttcctccca ggaggaaaaa gaatttcctg aatgccaaga aggccaacag ggccttgggc
     5161 ttggacacgc gtggcgatga gaacctgtac tttcagggcg gtatggtgag caagggcgag
     5221 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
     5281 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
     5341 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccttcggc
     5401 tacggcctgc agtgcttcgc ccgctacccc gaccacatga agcagcacga cttcttcaag
     5461 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac
     5521 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
     5581 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac
     5641 aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc
     5701 aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac
     5761 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag ctaccagtcc
     5821 gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
     5881 gccgccggga tcactctcgg catggacgag ctgtacaaga cgcgtggcgg aacaccagga
     5941 tctggcggag attacaagga cgacgatgac aagtgagtcg agatatctag acccagcttt
     6001 cttgtacaaa gtggttgata tccagcacag tggcggccgc tcgagtctag agggcccgcg
     6061 gttcgaaggt aagcctatcc ctaaccctct cctcggtctc gattctacgc gtaccggtta
     6121 gtaatgagtt taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
     6181 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
     6241 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
     6301 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
     6361 tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc
     6421 ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     6481 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     6541 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     6601 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtaccta
     6661 gaagttccta ttccgaagtt cctattctct agaaagtata ggaacttcct tggccaaaaa
     6721 gcctgaactc accgcgacgt ctgtcgagaa gtttctgatc gaaaagttcg acagcgtctc
     6781 cgacctgatg cagctctcgg agggcgaaga atctcgtgct ttcagcttcg atgtaggagg
     6841 gcgtggatat gtcctgcggg taaatagctg cgccgatggt ttctacaaag atcgttatgt
     6901 ttatcggcac tttgcatcgg ccgcgctccc gattccggaa gtgcttgaca ttggggaatt
     6961 cagcgagagc ctgacctatt gcatctcccg ccgtgcacag ggtgtcacgt tgcaagacct
     7021 gcctgaaacc gaactgcccg ctgttctgca gccggtcgcg gaggccatgg atgcgatcgc
     7081 tgcggccgat cttagccaga cgagcgggtt cggcccattc ggaccgcaag gaatcggtca
     7141 atacactaca tggcgtgatt tcatatgcgc gattgctgat ccccatgtgt atcactggca
     7201 aactgtgatg gacgacaccg tcagtgcgtc cgtcgcgcag gctctcgatg agctgatgct
     7261 ttgggccgag gactgccccg aagtccggca cctcgtgcac gcggatttcg gctccaacaa
     7321 tgtcctgacg gacaatggcc gcataacagc ggtcattgac tggagcgagg cgatgttcgg
     7381 ggattcccaa tacgaggtcg ccaacatctt cttctggagg ccgtggttgg cttgtatgga
     7441 gcagcagacg cgctacttcg agcggaggca tccggagctt gcaggatcgc cgcggctccg
     7501 ggcgtatatg ctccgcattg gtcttgacca actctatcag agcttggttg acggcaattt
     7561 cgatgatgca gcttgggcgc agggtcgatg cgacgcaatc gtccgatccg gagccgggac
     7621 tgtcgggcgt acacaaatcg cccgcagaag cgcggccgtc tggaccgatg gctgtgtaga
     7681 agtactcgcc gatagtggaa accgacgccc cagcactcgt ccgagggcaa aggaatagca
     7741 cgtactacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
     7801 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
     7861 ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     7921 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     7981 tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc
     8041 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     8101 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     8161 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     8221 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     8281 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     8341 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     8401 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     8461 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     8521 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     8581 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     8641 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     8701 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     8761 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     8821 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     8881 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     8941 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     9001 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     9061 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     9121 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     9181 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     9241 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     9301 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     9361 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     9421 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     9481 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     9541 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     9601 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     9661 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     9721 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     9781 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     9841 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     9901 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     9961 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
    10021 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
    10081 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
    10141 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg
    10201 atcgggagat ctcccgatcc cctatggtgc actctcagta caatctgctc tgatgccgca
    10261 tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc
    10321 aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg
    10381 gttaggcgtt ttgcgctgct tcg
//
