LOCUS       sequence-288057-       11904 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for human ACE2 with an N-terminal 
            (extracellular) c-myc tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 11904)
  AUTHORS   Chan KK, Dorosky D, Sharma P, Abbasi SA, Dye JM, Kranz DM, Herbert 
            AS, Procko E
  TITLE     Engineering human ACE2 to optimize binding to the spike protein of 
            SARS coronavirus 2.
  JOURNAL   Science. 2020 Sep 4;369(6508):1261-1265. doi: 
            10.1126/science.abc0870. Epub 2020 Aug 4.
  PUBMED    32753553
REFERENCE   2  (bases 1 to 11904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.abc0870"; journalName: "Science"; date: "2020-09-4-
            4"; volume: "369"; issue: "6508"; pages: "1261-1265"
COMMENT     Sequence Label: pCEP4-myc-ACE2
FEATURES             Location/Qualifiers
     source          1..11904
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(678..696)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        764..868
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(869..937,938..1729)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1087..1106)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1900..2488
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2389..2408
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2919..3064
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             3107..4144
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(4142..4161)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4186..4234
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4888..5267
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5268..5471
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5422..5442
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5468..5492
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             5574..5603
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    8008..8142
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8032..8051)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8086..8105
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(8285..8307)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8867..10656
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 gccaatgcaa cttggacgtt tttggggtct ccggacacca tctctatgtc ttggccctga
       61 tcctgagccg cccggggctc ctggtcttcc gcctcctcgt cctcgtcctc ttccccgtcc
      121 tcgtccatgg ttatcacccc ctcttctttg aggtccactg ccgccggagc cttctggtcc
      181 agatgtgtct cccttctctc ctaggccatt tccaggtcct gtacctggcc cctcgtcaga
      241 catgattcac actaaaagag atcaatagac atctttatta gacgacgctc agtgaataca
      301 gggagtgcag actcctgccc cctccaacag cccccccacc ctcatcccct tcatggtcgc
      361 tgtcagacag atccaggtct gaaaattccc catcctccga accatcctcg tcctcatcac
      421 caattactcg cagcccggaa aactcccgct gaacatcctc aagatttgcg tcctgagcct
      481 caagccaggc ctcaaattcc tcgtccccct ttttgctgga cggtagggat ggggattctc
      541 gggacccctc ctcttcctct tcaaggtcac cagacagaga tgctactggg gcaacggaag
      601 aaaagctggg tgcggcctgt gaggatcagc ttatcgatga taagctgtca aacatgagaa
      661 ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
      721 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
      781 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
      841 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
      901 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
      961 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     1021 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     1081 agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg
     1141 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     1201 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     1261 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     1321 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     1381 accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact
     1441 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     1501 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     1561 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     1621 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     1681 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     1741 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     1801 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     1861 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     1921 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     1981 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     2041 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     2101 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     2161 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     2221 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     2281 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     2341 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     2401 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     2461 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     2521 ggccttttgc tggccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat
     2581 ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc
     2641 catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccccgagatg
     2701 cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt
     2761 tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg
     2821 ttagcgaggt gccgccctgc ttcatccccg tggcccgttg ctcgcgtttg ctggcggtgt
     2881 ccccggaaga aatatatttg catgtcttta gttctatgat gacacaaacc ccgcccagcg
     2941 tcttgtcatt ggcgaattcg aacacgcaga tgcagtcggg gcggcgcggt ccgaggtcca
     3001 cttcgcatat taaggtgacg cgtgtggcct cgaacaccga gcgaccctgc agcgacccgc
     3061 ttaacagcgt caacagcgtg ccgcagatcc cggggggcaa tgagatatga aaaagcctga
     3121 actcaccgcg acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg tctccgacct
     3181 gatgcagctc tcggagggcg aagaatctcg tgctttcagc ttcgatgtag gagggcgtgg
     3241 atatgtcctg cgggtaaata gctgcgccga tggtttctac aaagatcgtt atgtttatcg
     3301 gcactttgca tcggccgcgc tcccgattcc ggaagtgctt gacattgggg aattcagcga
     3361 gagcctgacc tattgcatct cccgccgtgc acagggtgtc acgttgcaag acctgcctga
     3421 aaccgaactg cccgctgttc tgcagccggt cgcggaggcc atggatgcga tcgctgcggc
     3481 cgatcttagc cagacgagcg ggttcggccc attcggaccg caaggaatcg gtcaatacac
     3541 tacatggcgt gatttcatat gcgcgattgc tgatccccat gtgtatcact ggcaaactgt
     3601 gatggacgac accgtcagtg cgtccgtcgc gcaggctctc gatgagctga tgctttgggc
     3661 cgaggactgc cccgaagtcc ggcacctcgt gcacgcggat ttcggctcca acaatgtcct
     3721 gacggacaat ggccgcataa cagcggtcat tgactggagc gaggcgatgt tcggggattc
     3781 ccaatacgag gtcgccaaca tcttcttctg gaggccgtgg ttggcttgta tggagcagca
     3841 gacgcgctac ttcgagcgga ggcatccgga gcttgcagga tcgccgcggc tccgggcgta
     3901 tatgctccgc attggtcttg accaactcta tcagagcttg gttgacggca atttcgatga
     3961 tgcagcttgg gcgcagggtc gatgcgacgc aatcgtccga tccggagccg ggactgtcgg
     4021 gcgtacacaa atcgcccgca gaagcgcggc cgtctggacc gatggctgtg tagaagtact
     4081 cgccgatagt ggaaaccgac gccccagcac tcgtccggat cgggagatgg gggaggctaa
     4141 ctgaaacacg gaaggagaca ataccggaag gaacccgcgc tatgacggca ataaaaagac
     4201 agaataaaac gcacgggtgt tgggtcgttt gttcataaac gcggggttcg gtcccagggc
     4261 tggcactctg tcgatacccc accgagaccc cattggggcc aatacgcccg cgtttcttcc
     4321 ttttccccac cccacccccc aagttcgggt gaaggcccag ggctcgcagc caacgtcggg
     4381 gcggcaggcc ctgccatagc cactggcccc gtgggttagg gacggggtcc cccatgggga
     4441 atggtttatg gttcgtgggg gttattattt tgggcgttgc gtggggtcag gtccacgact
     4501 ggactgagca gacagaccca tggtttttgg atggcctggg catggaccgc atgtactggc
     4561 gcgacacgaa caccgggcgt ctgtggctgc caaacacccc cgacccccaa aaaccaccgc
     4621 gcggatttct ggcgtgccaa gctagtcgac caattctcat gtttgacagc ttatcatcgc
     4681 agatccgggc aacgttgttg ccattgctgc aggcgcagaa ctggtaggta tggaagatct
     4741 atacattgaa tcaatattgg caattagcca tattagtcat tggttatata gcataaatca
     4801 atattggcta ttggccattg catacgttgt atctatatca taatatgtac atttatattg
     4861 gctcatgtcc aatatgaccg ccatgttgac attgattatt gactagttat taatagtaat
     4921 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
     4981 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     5041 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     5101 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg
     5161 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact
     5221 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     5281 ggcagtacac caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
     5341 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     5401 gtaataaccc cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     5461 taagcagagc tcgtttagtg aaccgtcaga tctctagaag ctgggtacca gctgctagcg
     5521 ccaccatgaa gaccatcatc gccctgagct acatcttctg cctggtgttc gccgagcaaa
     5581 agctgatctc agaggaggac ctgggcagcc ctggcggtgc ctccaccatt gaggaacagg
     5641 ccaagacatt tttggacaag tttaaccacg aagccgaaga cctgttctat caaagttcac
     5701 ttgcttcttg gaattataac accaatatta ctgaagagaa tgtccaaaac atgaataatg
     5761 ctggggacaa atggtctgcc tttttaaagg aacagtccac acttgcccaa atgtatccac
     5821 tacaagaaat tcagaatctc acagtcaagc ttcagctgca ggctcttcag caaaatgggt
     5881 cttcagtgct ctcagaagac aagagcaaac ggttgaacac aattctaaat acaatgagca
     5941 ccatctacag tactggaaaa gtttgtaacc cagataatcc acaagaatgc ttattacttg
     6001 aaccaggttt gaatgaaata atggcaaaca gtttagacta caatgagagg ctctgggctt
     6061 gggaaagctg gagatctgag gtcggcaagc agctgaggcc attatatgaa gagtatgtgg
     6121 tcttgaaaaa tgagatggca agagcaaatc attatgagga ctatggggat tattggagag
     6181 gagactatga agtaaatggg gtagatggct atgactacag ccgcggccag ttgattgaag
     6241 atgtggaaca tacctttgaa gagattaaac cattatatga acatcttcat gcctatgtga
     6301 gggcaaagtt gatgaatgcc tatccttcct atatcagtcc aattggatgc ctccctgctc
     6361 atttgcttgg tgatatgtgg ggtagatttt ggacaaatct gtactctttg acagttccct
     6421 ttggacagaa accaaacata gatgttactg atgcaatggt ggaccaggcc tgggatgcac
     6481 agagaatatt caaggaggcc gagaagttct ttgtatctgt tggtcttcct aatatgactc
     6541 aaggattctg ggaaaattcc atgctaacgg acccaggaaa tgttcagaaa gcagtctgcc
     6601 atcccacagc ttgggacctg gggaagggcg acttcaggat ccttatgtgc acaaaggtga
     6661 caatggacga cttcctgaca gctcatcatg agatggggca tatccagtat gatatggcat
     6721 atgctgcaca accttttctg ctaagaaatg gagctaatga aggattccat gaagctgttg
     6781 gggaaatcat gtcactttct gcagccacac ctaagcattt aaaatccatt ggtcttctgt
     6841 cacccgattt tcaagaagac aatgaaacag aaataaactt cctgctcaaa caagcactca
     6901 cgattgttgg gactctgcca tttacttaca tgttagagaa gtggaggtgg atggtcttta
     6961 aaggggaaat tcccaaagac cagtggatga aaaagtggtg ggagatgaag cgagagatag
     7021 ttggggtggt ggaacctgtg ccccatgatg aaacatactg tgaccccgca tctctgttcc
     7081 atgtttctaa tgattactca ttcattcgat attacacaag gaccctttac caattccagt
     7141 ttcaagaagc actttgtcaa gcagctaaac atgaaggccc tctgcacaaa tgtgacatct
     7201 caaactctac agaagctgga cagaaactgt tcaatatgct gaggcttgga aaatcagaac
     7261 cctggaccct agcattggaa aatgttgtag gagcaaagaa catgaatgta aggccactgc
     7321 tcaactactt tgagccctta tttacctggc tgaaagacca gaacaagaat tcttttgtgg
     7381 gatggagtac cgactggagt ccatatgcag accaaagcat caaagtgagg ataagcctaa
     7441 aatcagctct tggagataaa gcatatgaat ggaacgacaa tgaaatgtac ctgttccgat
     7501 catctgttgc atatgctatg aggcagtact ttttaaaagt aaaaaatcag atgattcttt
     7561 ttggggagga ggatgtgcga gtggctaatt tgaaaccaag aatctccttt aatttctttg
     7621 tcactgcacc taaaaatgtg tctgatatca ttcctagaac tgaagttgaa aaggccatca
     7681 ggatgtcccg gagccgtatc aatgatgctt tccgtctgaa tgacaacagc ctagagtttc
     7741 tggggataca gccaacactt ggacctccta accagccccc tgtttccata tggctgattg
     7801 tttttggagt tgtgatggga gtgatagtgg ttggcattgt catcctgatc ttcactggga
     7861 tcagagatcg gaagaagaaa aataaagcaa gaagtggaga aaatccttat gcctccatcg
     7921 atattagcaa aggagaaaat aatccaggat tccaaaacac tgatgatgtt cagacctcct
     7981 tttagtaact cgaggccggc aaggccggat ccagacatga taagatacat tgatgagttt
     8041 ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct
     8101 attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt
     8161 cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc
     8221 tacaaatgtg gtatggctga ttatgatccg gctgcctcgc gcgtttcggt gatgacggtg
     8281 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
     8341 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca
     8401 tgaggtcgac tctagaggat cgatccccgc cccggacgaa ctaaacctga ctacgacatc
     8461 tctgcccctt cttcgcgggg cagtgcatgt aatcccttca gttggttggt acaacttgcc
     8521 aactgggccc tgttccacat gtgacacggg gggggaccaa acacaaaggg gttctctgac
     8581 tgtagttgac atccttataa atggatgtgc acatttgcca acactgagtg gctttcatcc
     8641 tggagcagac tttgcagtct gtggactgca acacaacatt gcctttatgt gtaactcttg
     8701 gctgaagctc ttacaccaat gctgggggac atgtacctcc caggggccca ggaagactac
     8761 gggaggctac accaacgtca atcagagggg cctgtgtagc taccgataag cggaccctca
     8821 agagggcatt agcaatagtg tttataaggc ccccttgtta accctaaacg ggtagcatat
     8881 gcttcccggg tagtagtata tactatccag actaacccta attcaatagc atatgttacc
     8941 caacgggaag catatgctat cgaattaggg ttagtaaaag ggtcctaagg aacagcgata
     9001 tctcccaccc catgagctgt cacggtttta tttacatggg gtcaggattc cacgagggta
     9061 gtgaaccatt ttagtcacaa gggcagtggc tgaagatcaa ggagcgggca gtgaactctc
     9121 ctgaatcttc gcctgcttct tcattctcct tcgtttagct aatagaataa ctgctgagtt
     9181 gtgaacagta aggtgtatgt gaggtgctcg aaaacaaggt ttcaggtgac gcccccagaa
     9241 taaaatttgg acggggggtt cagtggtggc attgtgctat gacaccaata taaccctcac
     9301 aaaccccttg ggcaataaat actagtgtag gaatgaaaca ttctgaatat ctttaacaat
     9361 agaaatccat ggggtgggga caagccgtaa agactggatg tccatctcac acgaatttat
     9421 ggctatgggc aacacataat cctagtgcaa tatgatactg gggttattaa gatgtgtccc
     9481 aggcagggac caagacaggt gaaccatgtt gttacactct atttgtaaca aggggaaaga
     9541 gagtggacgc cgacagcagc ggactccact ggttgtctct aacacccccg aaaattaaac
     9601 ggggctccac gccaatgggg cccataaaca aagacaagtg gccactcttt tttttgaaat
     9661 tgtggagtgg gggcacgcgt cagcccccac acgccgccct gcggttttgg actgtaaaat
     9721 aagggtgtaa taacttggct gattgtaacc ccgctaacca ctgcggtcaa accacttgcc
     9781 cacaaaacca ctaatggcac cccggggaat acctgcataa gtaggtgggc gggccaagat
     9841 aggggcgcga ttgctgcgat ctggaggaca aattacacac acttgcgcct gagcgccaag
     9901 cacagggttg ttggtcctca tattcacgag gtcgctgaga gcacggtggg ctaatgttgc
     9961 catgggtagc atatactacc caaatatctg gatagcatat gctatcctaa tctatatctg
    10021 ggtagcatag gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg
    10081 ggtagtatat gctatcctaa tttatatctg ggtagcatag gctatcctaa tctatatctg
    10141 ggtagcatat gctatcctaa tctatatctg ggtagtatat gctatcctaa tctgtatccg
    10201 ggtagcatat gctatcctaa tagagattag ggtagtatat gctatcctaa tttatatctg
    10261 ggtagcatat actacccaaa tatctggata gcatatgcta tcctaatcta tatctgggta
    10321 gcatatgcta tcctaatcta tatctgggta gcataggcta tcctaatcta tatctgggta
    10381 gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaattta tatctgggta
    10441 gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta
    10501 gtatatgcta tcctaatctg tatccgggta gcatatgcta tcctcatgca tatacagtca
    10561 gcatatgata cccagtagta gagtgggagt gctatccttt gcatatgccg ccacctccca
    10621 agggggcgtg aattttcgct gcttgtcctt ttcctgctgg ttgctcccat tcttaggtga
    10681 atttaaggag gccaggctaa agccgtcgca tgtctgattg ctcaccaggt aaatgtcgct
    10741 aatgttttcc aacgcgagaa ggtgttgagc gcggagctga gtgacgtgac aacatgggta
    10801 tgcccaattg ccccatgttg ggaggacgaa aatggtgaca agacagatgg ccagaaatac
    10861 accaacagca cgcatgatgt ctactgggga tttattcttt agtgcggggg aatacacggc
    10921 ttttaatacg attgagggcg tctcctaaca agttacatca ctcctgccct tcctcaccct
    10981 catctccatc acctccttca tctccgtcat ctccgtcatc accctccgcg gcagcccctt
    11041 ccaccatagg tggaaaccag ggaggcaaat ctactccatc gtcaaagctg cacacagtca
    11101 ccctgatatt gcaggtagga gcgggctttg tcataacaag gtccttaatc gcatccttca
    11161 aaacctcagc aaatatatga gtttgtaaaa agaccatgaa ataacagaca atggactccc
    11221 ttagcgggcc aggttgtggg ccgggtccag gggccattcc aaaggggaga cgactcaatg
    11281 gtgtaagacg acattgtgga atagcaaggg cagttcctcg ccttaggttg taaagggagg
    11341 tcttactacc tccatatacg aacacaccgg cgacccaagt tccttcgtcg gtagtccttt
    11401 ctacgtgact cctagccagg agagctctta aaccttctgc aatgttctca aatttcgggt
    11461 tggaacctcc ttgaccacga tgctttccaa accaccctcc ttttttgcgc ctgcctccat
    11521 caccctgacc ccggggtcca gtgcttgggc cttctcctgg gtcatctgcg gggccctgct
    11581 ctatcgctcc cgggggcacg tcaggctcac catctgggcc accttcttgg tggtattcaa
    11641 aataatcggc ttcccctaca gggtggaaaa atggccttct acctggaggg ggcctgcgcg
    11701 gtggagaccc ggatgatgat gactgactac tgggactcct gggcctcttt tctccacgtc
    11761 cacgacctct ccccctggct ctttcacgac ttccccccct ggctctttca cgtcctctac
    11821 cccggcggcc tccactacct cctcgacccc ggcctccact acctcctcga ccccggcctc
    11881 cactgcctcc tcgaccccgg cctc
//
