LOCUS       sequence-288083-       11712 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of SARS-CoV-2 protein; transient expression 
            and generate lentivirus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11712)
  AUTHORS   Gordon DE, Jang GM, Bouhaddou M, Xu J, Obernier K, White KM, O'Meara
            MJ, Rezelj VV, Guo JZ, Swaney DL, Tummino TA, Huttenhain R, Kaake 
            RM, Richards AL, Tutuncuoglu B, Foussard H, Batra J, Haas K, Modak 
            M, Kim M, Haas P, Polacco BJ, Braberg H, Fabius JM, Eckhardt M, 
            Soucheray M, Bennett MJ, Cakir M, McGregor MJ, Li Q, Meyer B, Roesch
            F, Vallet T, Mac Kain A, Miorin L, Moreno E, Naing ZZC, Zhou Y, Peng
            S, Shi Y, Zhang Z, Shen W, Kirby IT, Melnyk JE, Chorba JS, Lou K, 
            Dai SA, Barrio-Hernandez I, Memon D, Hernandez-Armenta C, Lyu J, 
            Mathy CJP, Perica T, Pilla KB, Ganesan SJ, Saltzberg DJ, Rakesh R, 
            Liu X, Rosenthal SB, Calviello L, Venkataramanan S, Liboy-Lugo J, 
            Lin Y, Huang XP, Liu Y, Wankowicz SA, Bohn M, Safari M, Ugur FS, Koh
            C, Savar NS, Tran QD, Shengjuler D, Fletcher SJ, O'Neal MC, Cai Y, 
            Chang JCJ, Broadhurst DJ, Klippsten S, Sharp PP, Wenzell NA, 
            Kuzuoglu-Ozturk D, Wang HY, Trenker R, Young JM, Cavero DA, Hiatt J,
            Roth TL, Rathore U, Subramanian A, Noack J, Hubert M, Stroud RM, 
            Frankel AD, Rosenberg OS, Verba KA, Agard DA, Ott M, Emerman M, Jura
            N, von Zastrow M, Verdin E, Ashworth A, Schwartz O, d'Enfert C, 
            Mukherjee S, Jacobson M, Malik HS, Fujimori DG, Ideker T, Craik CS, 
            Floor SN, Fraser JS, Gross JD, Sali A, Roth BL, Ruggero D, Taunton 
            J, Kortemme T, Beltrao P, Vignuzzi M, Garcia-Sastre A, Shokat KM, 
            Shoichet BK, Krogan NJ
  TITLE     A SARS-CoV-2 protein interaction map reveals targets for drug 
            repurposing.
  JOURNAL   Nature. 2020 Jul;583(7816):459-468. doi: 10.1038/s41586-020-2286-9. 
            Epub 2020 Apr 30.
  PUBMED    32353859
REFERENCE   2  (bases 1 to 11712)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-020-2286-9"; journalName: "Nature"; date: "2020-07";
            volume: "583"; issue: "7816"; pages: "459-468"
COMMENT     Sequence Label: pLVX-EF1alpha-SARS-CoV-2-nsp12-2xStrep-IRES-Puro
FEATURES             Location/Qualifiers
     source          1..11712
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2337..3519
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2568..3510
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3467..3487
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             6367..6390
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             6427..6450
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     misc_feature    join(6462..7036,7037..7039,7040..7048)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6629..6646)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6856..6875
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7068..7667
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7068..7087)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7564..7584
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7681..8269
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7734..7754)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8152..8163)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8476..9109
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9237..9253)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9237..9253)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9250..9272)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9261..9277
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9285..9291,9292..9309,9310..9315))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9330..9351
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9468..9485)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9639..10227)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9719..9738)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10398..11189,11190..11258))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11021..11040
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11259..11363)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    11411..11545
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11448..11467)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11502..11521
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgagtaa ttcatacaaa aggactcgcc cctgccttgg
     2221 ggaatcccag ggaccgtcgt taaactccca ctaacgtaga acccagagat cgctgcgttc
     2281 ccgccccctc acccgcccgc tctcgtcatc actgaggtgg agaagagcat gcgtgaggct
     2341 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag
     2401 gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg
     2461 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag
     2521 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt
     2581 gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt
     2641 ccacgcccct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
     2701 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
     2761 cttgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
     2821 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
     2881 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
     2941 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
     3001 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
     3061 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
     3121 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
     3181 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
     3241 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
     3301 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     3361 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     3421 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     3481 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gatctatttc cggtgaattc
     3541 gccgccacca tgtcagcaga cgcacaaagt tttctgaacc gggtatgcgg agtcagtgcg
     3601 gcgaggctta cgccctgtgg tacgggaaca tcaacggacg ttgtttaccg cgccttcgac
     3661 atatacaatg ataaagtcgc cggcttcgca aagttcctta aaacgaattg ctgtaggttc
     3721 caagaaaagg acgaggatga taatttgata gactcctact tcgtggtcaa acgacacacg
     3781 ttttcaaatt atcagcacga ggaaacgata tataatcttc ttaaagactg ccctgcagtg
     3841 gcaaaacacg acttctttaa gttccgcatt gacggggata tggtccccca tatctcccga
     3901 cagagattga cgaaatatac aatggcggat ctggtgtacg ccctgagaca cttcgatgag
     3961 ggcaattgtg atacactcaa ggagattctc gtgacctaca attgttgcga tgatgactat
     4021 ttcaataaga aagactggta cgacttcgtc gaaaaccctg atatattgag ggtgtacgca
     4081 aacctgggtg aacgagtaag acaggcactt ctgaaaaccg tccaattctg cgacgctatg
     4141 agaaacgcgg gcattgttgg tgtgcttacg ctggataacc aagacctcaa tggtaattgg
     4201 tacgattttg gggacttcat ccaaaccact cctggctcag gcgttcccgt ggtagacagc
     4261 tactattctt tgttgatgcc aattcttaca ctcacaaggg ccctgaccgc tgagtcacac
     4321 gtcgatacgg acttgaccaa gccctacatc aagtgggatc ttctgaagta cgactttacc
     4381 gaggaacgat tgaagctttt tgatcgctac tttaaatact gggatcagac gtaccaccca
     4441 aactgcgtga attgcctgga tgaccgatgt atactccatt gcgcgaactt taacgttttg
     4501 ttctctacag tatttcctcc gacgagcttc ggaccgcttg tgagaaagat attcgtcgac
     4561 ggcgtcccat ttgttgtcag cactggttac cactttcgcg agttgggggt ggtccacaat
     4621 caggatgtca atttgcattc ctccaggctt tcttttaaag agttgcttgt ttatgccgct
     4681 gaccccgcga tgcatgcagc ctctgggaat cttttgctgg acaaaaggac gacgtgcttt
     4741 agcgttgctg cccttacaaa taacgtagca tttcaaaccg taaaacccgg taattttaat
     4801 aaagattttt atgattttgc agtctctaag ggttttttca aggaggggag tagtgtagag
     4861 cttaaacatt tttttttcgc tcaggatggt aacgctgcga tcagcgacta cgactactat
     4921 cgatacaatc tcccgacgat gtgtgatatc aggcagttgt tgttcgttgt agaagtggtt
     4981 gataagtatt ttgactgtta tgacggcggc tgtatcaatg ccaaccaggt cattgtgaat
     5041 aacctcgata aatctgccgg gttccccttt aacaagtggg ggaaggcgcg actgtactac
     5101 gacagcatgt cttacgaaga tcaggacgca ctctttgcat acacaaagag gaatgtgata
     5161 ccgactataa ctcaaatgaa ccttaagtat gcaatcagcg cgaaaaatcg ggctaggact
     5221 gttgccggag ttagcatatg tagtaccatg acaaaccgac agttccacca gaagttgctg
     5281 aaatcaattg ctgcgacccg gggtgcgact gtagtaatcg ggacatcaaa gttctacggc
     5341 gggtggcata acatgctcaa gacggtctac tcagacgttg agaatccgca tcttatgggt
     5401 tgggattatc caaagtgtga cagggcgatg cccaacatgt tgcggataat ggcctccttg
     5461 gtacttgcac ggaaacatac aacctgctgt agtttgtcac accgatttta ccgactggca
     5521 aacgaatgtg ctcaggttct cagtgaaatg gtaatgtgtg gcggctccct ctacgttaag
     5581 cccggtggca caagctctgg agacgcaact actgcctatg caaacagtgt attcaacatc
     5641 tgccaggctg tcacggccaa tgttaacgca ttgctttcca ctgacggaaa taagattgca
     5701 gacaaatacg tgagaaattt gcaacataga ctctacgaat gcctctatcg caatcgcgat
     5761 gttgatacgg acttcgttaa tgagttctat gcttacttgc gaaagcactt ctccatgatg
     5821 attctgtcag acgacgctgt agtatgcttt aattcaacgt atgcctccca gggtctggtc
     5881 gcgtccataa aaaattttaa aagcgtcctg tattaccaaa ataacgtttt catgagcgaa
     5941 gcgaaatgtt ggaccgagac tgatcttact aagggccctc atgagttctg ctcacaacac
     6001 actatgttgg tcaagcaggg cgacgattac gtgtatcttc cttatcccga tcctagtagg
     6061 attcttggag caggttgctt cgtggatgat atcgtcaaaa cggacggcac cctcatgata
     6121 gagaggttcg tctctttggc tatagacgcg tatccgctta ctaaacatcc taaccaagaa
     6181 tacgcggatg tattccatct ttatctgcag tatatcagaa agttgcatga tgagctgacc
     6241 ggtcacatgt tggacatgta tagcgtgatg cttaccaatg acaatacttc acggtactgg
     6301 gaacctgaat tttacgaggc gatgtatacg cctcacaccg tcctgcagct cgaaggcggc
     6361 gggggatgga gccatccaca attcgagaaa ggcggtggtt caggaggagg tagcgggggt
     6421 ggatcatggt cacatccgca gtttgaaaag taaggatccc gcccctctcc ctcccccccc
     6481 cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta
     6541 ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc
     6601 ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat
     6661 gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc
     6721 ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt
     6781 gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt
     6841 gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag
     6901 aaggtacccc attgtatggg atctgatctg gggcctcggt gcacatgctt tacatgtgtt
     6961 tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga
     7021 aaaacacgat gataagcttg ccacaaccca caaggagacg accttccatg accgagtaca
     7081 agcccacggt gcgcctcgcc acccgcgacg acgtcccccg ggccgtacgc accctcgccg
     7141 ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga cccggaccgc cacatcgagc
     7201 gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt
     7261 gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg
     7321 gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg
     7381 cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc
     7441 tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc
     7501 tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc
     7561 cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc
     7621 ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctagacg cgtctggaac
     7681 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     7741 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     7801 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     7861 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     7921 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     7981 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     8041 ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc
     8101 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     8161 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     8221 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggaattaat
     8281 tctgcagtcg agacctagaa aaacatggag caatcacaag tagcaataca gcagctacca
     8341 atgctgattg tgcctggcta gaagcacaag aggaggagga ggtgggtttt ccagtcacac
     8401 ctcaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     8461 aagaaaagag gggactggaa gggctaattc actcccaacg aagacaagat atccttgatc
     8521 tgtggatcta ccacacacaa ggctacttcc ctgattagca gaactacaca ccagggccag
     8581 gggtcagata tccactgacc tttggatggt gctacaagct agtaccagtt gagccagata
     8641 aggtagaaga ggccaataaa ggagagaaca ccagcttgtt acaccctgtg agcctgcatg
     8701 ggatggatga cccggagaga gaagtgttag agtggaggtt tgacagccgc ctagcatttc
     8761 atcacgtggc ccgagagctg catccggagt acttcaagaa ctgctgatat cgagcttgct
     8821 acaagggact ttccgctggg gactttccag ggaggcgtgg cctgggcggg actggggagt
     8881 ggcgagccct cagatcctgc atataagcag ctgctttttg cctgtactgg gtctctctgg
     8941 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     9001 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     9061 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca
     9121 tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg
     9181 ccttgacatt gctagcgttt accgtcgacc tctagctaga gcttggcgta atcatggtca
     9241 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     9301 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     9361 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     9421 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     9481 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     9541 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     9601 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     9661 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     9721 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     9781 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     9841 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     9901 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     9961 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
    10021 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
    10081 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
    10141 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
    10201 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
    10261 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
    10321 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
    10381 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
    10441 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
    10501 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
    10561 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
    10621 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
    10681 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
    10741 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
    10801 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
    10861 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
    10921 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
    10981 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
    11041 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
    11101 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
    11161 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
    11221 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
    11281 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
    11341 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga
    11401 tcgggagatc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
    11461 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
    11521 caatgtatct tatcatgtct ggatcaactg gataactcaa gctaaccaaa atcatcccaa
    11581 acttcccacc ccatacccta ttaccactgc caattacctg tggtttcatt tactctaaac
    11641 ctgtgattcc tctgaattat tttcatttta aagaaattgt atttgttaaa tatgtactac
    11701 aaacttagta gt
//
