LOCUS       sequence-288120-        8396 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Doxycycline Inducible Expression of mCherry-2A-FLAG-BRD4 long short 
            missing its extra-terminal domain (ET).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8396)
  AUTHORS   Edwards DS, Maganti R, Tanksley JP, Luo J, Park JJH, 
            Balkanska-Sinclair E, Ling J, Floyd SR
  TITLE     BRD4 Prevents R-Loop Formation and Transcription-Replication 
            Conflicts by Ensuring Efficient Transcription Elongation.
  JOURNAL   Cell Rep. 2020 Sep 22;32(12):108166. doi: 
            10.1016/j.celrep.2020.108166.
  PUBMED    32966794
REFERENCE   2  (bases 1 to 8396)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2020-09-22"; volume: "32(12):108166. doi"; pages: " 
            10.1016/j.celrep.2020.108166"
COMMENT     Sequence Label: pCW57-mCherry-2A-BRD4 Iso CdelET
FEATURES             Location/Qualifiers
     source          1..8396
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..227
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             228..408
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    455..580
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1073..1306
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1491..1535
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1748..1865
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1919..2233
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    1927..1945
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1963..1981
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1998..2016
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2034..2052
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2070..2088
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2105..2123
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2141..2159
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2206..2230
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    complement(2266..2299)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             2302..3009
                     /codon_start=1
                     /product="derivative of the monomeric red fluorescent 
                     protein mCherry"
                     /label=mCherry2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFNWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQCRTMGWEASTERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVDIKLDILSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2447..2465)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     protein_bind    complement(3010..3042)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             3052..3105
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3157..3180
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(5420..5431)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5636..5869
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5941..6062
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5978..5997)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6032..6051
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6102..6237
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        6237..6308
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6309..6377,6378..7169)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6527..6546)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7340..7928
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7829..7848
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8082..8099
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8216..8237
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8252..8257,8258..8275,8276..8282)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8290..8306
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8295..8317
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8314..8330
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8314..8330
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8349..8369
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8351..8369
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
       61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
      121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
      181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
      241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
      901 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
      961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1441 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1681 agacccacct cccaaccccg aggggacaat tctcgacctc gagacaaatg gcagtattca
     1741 tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag
     1801 acataatagc aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa
     1861 attttcgggt ttattacagg gacagcagag atccactttg gccgcgaatc gatatgtcga
     1921 gtttactccc tatcagtgat agagaacgta tgtcgagttt actccctatc agtgatagag
     1981 aacgatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgagtt tactccctat
     2041 cagtgataga gaacgtatgt cgagtttact ccctatcagt gatagagaac gtatgtcgag
     2101 tttatcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
     2161 cgtatgtcga ggtaggcgtg tacggtggga ggcctatata agcagagctc gtttagtgaa
     2221 ccgtcagatc gcctggagaa ttggctagcg ctaccggtcg ccaccataac ttcgtatagc
     2281 atacattata cgaagttatt aatggtgagc aagggcgagg aggataacat ggccatcatc
     2341 aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag
     2401 atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg
     2461 accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc
     2521 tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc
     2581 gagggcttca attgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc
     2641 caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac
     2701 ttcccctccg acggccccgt aatgcagtgt cgtaccatgg gctgggaggc ctccactgag
     2761 cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag
     2821 gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag
     2881 ctgcccggcg cctacaacgt cgacatcaag ttggacatcc tttcccacaa cgaggactac
     2941 accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag
     3001 ctttataaga taacttcgta tagcatacat tatacgaagt taggttctgg tgaaggtaga
     3061 ggttctctcc tcacttgtgg tgatgttgaa gaaaaccctg gtccacgaac gcgttctgac
     3121 actatgtaca agtccggact cagatctcga gcgatggatt acaaggatga cgacgataag
     3181 ggatctatgt ctgcggagag cggccctggg acgagattga gaaatctgcc agtaatgggg
     3241 gatggactag aaacttccca aatgtctaca acacaggccc aggcccaacc ccagccagcc
     3301 aacgcagcca gcaccaaccc cccgccccca gagacctcca accctaacaa gcccaagagg
     3361 cagaccaacc aactgcaata cctgctcaga gtggtgctca agacactatg gaaacaccag
     3421 tttgcatggc ctttccagca gcctgtggat gccgtcaagc tgaacctccc tgattactac
     3481 aaaattatca agactccgat ggacatggga acaataaaga agcgcttgga aaacaactat
     3541 tactggaatg ctcaggaatg tatccaggac ttcaacacta tgtttacaaa ttgttacatc
     3601 tacaacaagc ctggagatga catagtctta atggcagaag ctctggaaaa gctcttcttg
     3661 caaaaaataa atgagctacc cacagaagag acagaaataa tgatcgtaca agcaaaagga
     3721 agaggacgtg ggaggaaaga aacagggaca gcaaaacctg gcgtttccac ggtaccaaac
     3781 acaactcaag catcgactcc tccgcagacc cagacccctc agccgaatcc tcctcctgtg
     3841 caggccacgc ctcacccctt ccctgccgtc accccggacc tcatcgtcca gacccctgtc
     3901 atgacagtgg tgcctcccca gccactgcag acgcccccgc cagtgccccc ccagccacaa
     3961 cccccacccg ctccagctcc ccagcccgta cagagccacc cacccatcat cgcggccacc
     4021 ccacagcctg tgaagacaaa gaagggagtg aagaggaaag cagacaccac cacccccacc
     4081 accattgacc ccattcacga gccaccctcg ctgcccccgg agcccaagac caccaagctg
     4141 ggccagcggc gggagagcag ccggcctgtg aaacctccaa agaaggacgt gcccgactct
     4201 cagcagcacc cagcaccaga gaagagcagc aaggtctcgg agcagctcaa gtgctgcagc
     4261 ggcatcctca aggagatgtt tgccaagaag cacgccgcct acgcctggcc cttctacaag
     4321 cctgtggacg tggaggcact gggcctacac gactactgtg acatcatcaa gcaccccatg
     4381 gacatgagca caatcaagtc taaactggag gcccgtgagt accgtgatgc tcaggagttt
     4441 ggtgctgacg tccgattgat gttctccaac tgctataagt acaaccctcc tgaccatgag
     4501 gtggtggcca tggcccgcaa gctccaggat gtgttcgaaa tgcgctttgc caagatgccg
     4561 gacgagcctg aggagccagt ggtggccgtg tcctccccgg cagtgccccc tcccaccaag
     4621 gttgtggccc cgccctcatc cagcgacagc agcagcgata gctcctcgga cagtgacagt
     4681 tcgactgatg actctgagga ggagcgagcc cagcggctgg ctgagctcca ggagcagctc
     4741 aaagccgtgc acgagcagct tgcagccctc tctcagcccc agcagaacaa accaaagaaa
     4801 aaggagaaag acaagaagga aaagaaaaaa gaaaagcaca aaaggaaaga ggaagtggaa
     4861 gagaataaaa aaagcaaagc caaggaacct cctcctaaaa agacgaagaa aaataatagc
     4921 agcaacagca atgtgagcaa gaaggagcca gcgcccatga agagcaagcc ccctcccacg
     4981 tatactagtg ctgagaaagt tgatgtgatt gccggctcct ccaagatgaa gggcttctcg
     5041 tcctcagagt cggagagctc cagtgagtcc agctcctctg acagcgaaga ctccgaaaca
     5101 ggtcctgcct aatcaggatc caccggatct agataactga tcataatcag ccataccaca
     5161 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cccagctcct
     5221 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     5281 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     5341 gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     5401 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     5461 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     5521 ttgggccgcc tccccgcctg tttcgcctcg gcgtccggac tagaggtacc tttaagacca
     5581 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa
     5641 gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg
     5701 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     5761 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     5821 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca
     5881 tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg
     5941 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     6001 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     6061 tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc
     6121 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     6181 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggccttt
     6241 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     6301 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     6361 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     6421 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     6481 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     6541 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     6601 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     6661 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     6721 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     6781 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     6841 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     6901 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     6961 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     7021 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     7081 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     7141 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     7201 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     7261 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     7321 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     7381 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     7441 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     7501 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     7561 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     7621 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     7681 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     7741 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     7801 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     7861 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     7921 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     7981 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     8041 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     8101 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     8161 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     8221 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     8281 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     8341 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa gcttaa
//
