LOCUS       sequence-288176-         935 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pET vector for IPTG-inducible, T7-based expression of the 
            exonuclease deficient version of RTX bearing a C-terminal 6xHis tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Bhadra S, Maranhao AC, Paik I, Ellington AD
  TITLE     A one-enzyme RT-qPCR assay for SARS-CoV-2, and procedures for 
            reagent production
  JOURNAL   BioProtocol 2021
REFERENCE   2  (bases 1 to 935)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BioProtocol
            2021"
COMMENT     Sequence Label: pET_RTX(exo-)-6xHis
FEATURES             Location/Qualifiers
     source          1..935
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             11..33
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 ctagaataat tttgtttaac tttaagaagg agatatacat atgattcttg acacggatta
       61 catcacggaa gacggcaagc cggttatccg tattttcaag aaagaaaacg gcgaattcaa
      121 gattgaatac gatcggacat ttgaaccgta cctgtacgct ctcctcaagg atgatagcgc
      181 aatcgaagaa gtgaaaaaaa tcaccgcaga gcggcatggc acagtggtaa cagttaagcg
      241 ggtcgagaaa gtgcagaaga agttcttagg ccggccagtc gaagtatgga aattatactt
      301 cacacatcca caggacgttc cggcgatcat ggataagatt cgggagcatc cggcggtaat
      361 cgatatctat gaatacgata ttccgttcgc tattcgctac cttattgaca aaggtttagt
      421 tccaatggag ggtgatgagg aacttaaact gttagcattc gatatcgaaa cactttatca
      481 cgaaggtgaa gagtttgccg aaggtccgat tttaatgatc tcgtacgccg atgaagaagg
      541 cgcacgcgta attacgtgga aaaatgtgga cctcccgtac gtagacgtag tgagcactga
      601 gcgcgagatg attaaacgtt tccttcgggt agtaaaagaa aaagacccag acgtgctgat
      661 tacgtatgac ggcgacaact ttgattttgc ctatctcaag aagcgttgcg aaaagttagg
      721 cattaatttc gccctgggtc gggacggttc agagccgaaa attcagcgga tgggcgaccg
      781 ctttgctgtg gaggtaaaag gtcgcatcca tttcgattta tatccggtta tccggcgcac
      841 catcaacttg ccgacttaca cacttgaagc agtttacgaa gcggtgttcg gccaaccaaa
      901 agaaaaggnt tatgccgagg agattaccnc cgcat
//
