LOCUS       sequence-288425-        9784 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmAGO2-RC.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9784)
  AUTHORS   Rehwinkel J, Natalin P, Stark A, Brennecke J, Cohen SM, Izaurralde E
  TITLE     Genome-wide analysis of mRNAs regulated by Drosha and Argonaute 
            proteins in Drosophila melanogaster.
  JOURNAL   Mol Cell Biol. 2006 Apr . 26(8):2965-75.
  PUBMED    16581772
REFERENCE   2  (bases 1 to 9784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2006 Apr . 26(8):2965-75."
COMMENT     Sequence Label: pAc5.1B-EGFP-DmAGO2-RC_B
FEATURES             Location/Qualifiers
     source          1..9784
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             join(2588..2590,2591..2593,2594..3304)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2588..2590,2591..2593,2594..3304)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2633..2654)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2894..2913)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3241..3262
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             7069..7110
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             7120..7137
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7160..7177)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7210..7344
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7234..7253)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7288..7307
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(7966..8554)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8046..8065)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8725..9516,9517..9585))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9348..9367
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9586..9690)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9758..9776
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     2641 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
     2701 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     2761 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca
     2821 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     2881 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     2941 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     3001 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
     3061 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
     3121 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     3181 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca
     3241 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     3301 caagccaagc ttgaattctg cagatatcat gcactttcca attaccaccc cagagaacaa
     3361 gaaaggagga caggatagcg ctgcagcacc acagccccag cagcagcaaa agcaacaaca
     3421 acaacggcag caacaaccac agcagctgca acaaccacag cagctgcaac aaccacagca
     3481 gcagcaacaa caacaaccgc atcagcaaca acaacaaagt tcgagacaac agccatccac
     3541 aagctcaggc ggatcccgtg catctgggtt ccagcaagga ggccaacagc agaaatccca
     3601 agacgcagaa ggatggactg cacagaaaaa acaaggcaaa cagcaggtac aagggtggac
     3661 taaacaaggt caacaaggtg gccatcagca aggacgacaa gggcaagacg gtggctacca
     3721 acagcgtccg cctggacaac aacaaggtgg ccatcagcaa ggacgacaag ggcaagaagg
     3781 tggctaccaa cagcgtccgc ctggacaaca acaaggtggc catcagcaag gacgacaagg
     3841 gcaagaaggt ggctaccaac agcgtccgtc tggacaacaa caaggtggcc atcagcaagg
     3901 acgacaaggg caagaaggtg gctaccaaca gcgtccgcct ggacaacaac aaggtggcca
     3961 tcagcaagga cgacaagggc aagaaggtgg ctaccaacag cgtccgtctg gacaacaaca
     4021 aggtggccat cagcaaggac gacaagggca agaaggtggc taccaacagc gtccgcctgg
     4081 acaacaacaa ggtggccatc agcaaggacg acaagggcaa gaaggtggct accaacagcg
     4141 tccgcctgga caacaacaag gtggccatga gcaaggacga caagggcaag aaggtggcta
     4201 ccaacagcgt ccgtctggac aacaacaagg tggccatcag caaggacgac aagggcaaga
     4261 aggtggctac caacagcgtc cgtctggaca acaacaaggt ggccatcagc aaggacgaca
     4321 agggcaagaa ggtggctacc aacagcgtcc gtctggacaa caacaaggtg gccatcagca
     4381 aggacgacaa gggcaagaag gtggctacca acagcgtccg tctggacaac aacaaggtgg
     4441 ccatcagcaa ggacgacaag ggcaagaagg tggctaccaa cagcgtccgc ctggacaaca
     4501 accaaaccaa acccagagcc aaggccaata ccaatctcgt ggaccacctc agcaacagca
     4561 ggctgcccca ttaccattac cgcctcagcc agccgggagc attaagcgcg gaacaatcgg
     4621 caaacccggg caagtaggca tcaactatct ggaccttgac ctgtccaaaa tgccttctgt
     4681 ggcataccac tatgacgtga agatcatgcc agagcgtccc aaaaagtttt acagacaggc
     4741 atttgaacaa tttcgggtgg accaattggg cggagcagtt cttgcctatg atggcaaagc
     4801 ctcttgctac tcggtggata agctgccgtt aaatagccaa aatccggaag tgactgtgac
     4861 agatcgtaat ggtcgtactt tgcgctacac gatcgaaatc aaggagactg gtgactcgac
     4921 cattgacctt aaatcgttaa ctacttatat gaatgaccga attttcgaca agcccatgcg
     4981 agcaatgcag tgcgtggagg ttgttttggc ttcaccctgc cacaacaaag ccatccgtgt
     5041 cggtcgttcc ttctttaaga tgtccgatcc taataatcgt cacgaactgg atgatggata
     5101 cgaggccctg gtcggtctgt atcaggcatt tatgctgggc gataggccat ttttaaatgt
     5161 agatatatcg cacaaatcct ttccgatttc aatgccgatg atcgaatatt tggagcgatt
     5221 tagcttaaag gctaaaatca acaatacaac gaatttagat tattcgcgac gcttccttga
     5281 gccattcctc agaggcatta atgtggttta cacgcctcct cagtcgtttc aaagcgctcc
     5341 aagggtatac cgtgtaaatg gtctttctcg tgccccggcc agcagtgaga ccttcgaaca
     5401 cgatgggaag aaggtcacca ttgcgtccta cttccacagt cgcaactacc cattgaagtt
     5461 tccccaactc cattgtctga acgttggatc ttcaatcaag agtattctgc tgcccatcga
     5521 gctatgcagc atcgaggaag gtcaggccct aaaccgcaag gatggagcaa ctcaggtggc
     5581 caatatgata aagtacgcag ccacatcgac gaacgtgcga aagcgcaaga ttatgaactt
     5641 gctgcaatac ttccagcaca acctggatcc gaccatcagt cgctttggca tccgcattgc
     5701 caacgatttt attgtggtaa gcacccgcgt cctaaaccca cctcaggttg aatatcatag
     5761 taagaggttt actatggtga agaacgggtc gtggcgcatg gatggcatga agtttctgga
     5821 gcccaagccc aaggcgcaca agtgtgcggt cttgtattgc gatccgagga gtggtcgcaa
     5881 aatgaactat gcccagctga atgacttcgg gaacctaata atatcccaag gcaaggcagt
     5941 caacataagc ttggattctg atgtgacata cagaccgttc acggatgacg aacgcagcct
     6001 agacactatt ttcgcggatc tgaagcgcag ccagcacgat ctggcaattg tgattattcc
     6061 tcagtttaga atttcttacg atacaattaa gcagaaggcc gagctgcagc atggaatttt
     6121 gacgcaatgc attaagcagt tcaccgtgga acgaaagtgt aataatcaga cgattggaaa
     6181 tattctactt aagatcaact ccaagctgaa cggcatcaac cacaagatca aggatgatcc
     6241 tcgtctgccg atgatgaaga acaccatgta cattggagcc gatgtgaccc atccctctcc
     6301 cgatcagcgc gagattccca gtgtggtcgg agtagcagcc tcacacgatc cctacggagc
     6361 cagttataac atgcaatatc gtttgcagcg aggggctctg gaggagattg aggacatgtt
     6421 ctcgattact ttggagcact tgcgcgtgta taaagagtac cgtaacgcct atcctgatca
     6481 tatcatctac taccgagatg gcgtgagcga cggccagttt ccgaaaatca aaaacgagga
     6541 actgaggtgt attaaacaag cctgtgacaa ggtgggctgt aaacccaaga tttgctgcgt
     6601 gattgtggtg aagcgtcatc acactcgctt ctttcccagc ggcgacgtaa cgacatcgaa
     6661 caagttcaac aacgtggacc ccggaaccgt ggtcgatcgc accattgtgc atcctaacga
     6721 gatgcagttc ttcatggtca gccaccaggc catccagggc acggccaagc caacacgata
     6781 caatgtgatt gagaacacag gcaatcttga catcgacttg ttgcagcagt tgacctacaa
     6841 cctgtgccac atgttccctc gttgcaatcg ctcggtttct tatccggctc cggcctattt
     6901 agcccatttg gtagctgctc gtggacgcgt ttatctgact ggcaccaaca ggttcctgga
     6961 tttgaagaag gagtacgcaa agcgaacgat tgtccccgaa ttcatgaaga aaaaccccat
     7021 gtactttgtc tgagcggccg ctcgagtcta gagggcccgc ggttcgaagg taagcctatc
     7081 cctaaccctc tcctcggtct cgattctacg cgtaccggtc atcatcacca tcaccattga
     7141 gtttaaaccc gctgatcagc ctcgactgtg ccttctaagg cctgagctcg ctgatcagcc
     7201 tcgatcgagg atccagacat gataagatac attgatgagt ttggacaaac cacaactaga
     7261 atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
     7321 attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt
     7381 cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtatggct
     7441 gattatgatc agtcgaccga tgcccttgag agccttcaac ccagtcagct ccttccggtg
     7501 ggcgcggggc atgactatcg tcgccgcact tatgactgtc ttctttatca tgcaactcgt
     7561 aggacaggtg ccggcagcgc tctgggtcat tttcggcgag gaccgctttc gctggagcgc
     7621 gacgatgatc ggcctgtcgc ttgcggtatt cggaatcttg cacgccctcg ctcaagcctt
     7681 cgtcactggt cccgccacca aacgtttcgg cgagaagcag gccattatcg ccggcatggc
     7741 ggccgacgcg ctgggctacg tcttgctggc gttcgcgacg cgaggctgga tggccttccc
     7801 cattatgatt cttctcgctt ccggcggcat cgggatgccc gcgttgcagg ccatgctgtc
     7861 caggcaggta gatgacgacc atcagggaca gcttcaagga tcgctcgcgg ctcttaccag
     7921 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     7981 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     8041 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     8101 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     8161 atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     8221 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     8281 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     8341 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     8401 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     8461 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     8521 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     8581 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     8641 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     8701 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     8761 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     8821 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     8881 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     8941 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     9001 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg
     9061 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     9121 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     9181 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     9241 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     9301 atagtgtatg cggcgaccga gttgctcttg cccggcgtca acacgggata ataccgcgcc
     9361 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     9421 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     9481 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     9541 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     9601 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     9661 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     9721 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
     9781 tcgt
//
