LOCUS       sequence-288648-        6847 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmMe31B-mut1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6847)
  AUTHORS   Tritschler F, Braun JE, Eulalio A, Truffault V, Izaurralde E, 
            Weichenrieder O
  TITLE     Structural basis for the mutually exclusive anchoring of P body 
            components EDC3 and Tral to the DEAD box protein DDX6/Me31B.
  JOURNAL   Mol Cell. 2009 Mar 13. 33(5):661-8.
  PUBMED    19285948
REFERENCE   2  (bases 1 to 6847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2009 Mar 13. 33(5):661-8."
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmMe31B-mut1_E
FEATURES             Location/Qualifiers
     source          1..6847
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        12..2524
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2544..2609
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2634..2660
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4088..4129
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4139..4156
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4179..4196)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4229..4363
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4253..4272)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4307..4326
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(4985..5573)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5065..5084)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5744..6535,6536..6604))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6367..6386
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6605..6709)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6777..6795
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ctaaaaaaaa tcatgaatgg catcaactct gaatcaaatc tttgcagatg cacctacttc
       61 tcatttccac tgtcacatca tttttccaga tctcgctgcc tgttatgtgg cccacaaacc
      121 aagacacgtt ttatggccat taaagctggc tgatcgtcgc caaacaccaa atacatatca
      181 atatgtacat tcgagaaaga agcgatcaaa gaagcgtctt cgggcgagta ggagaatgcg
      241 gaggagaagg agaacgagct gatctagtat ctctccacaa tccaatgcca actgaccaac
      301 tggccatatt cggagcaatt tgaagccaat ttccatcgcc tggcgatcgc tccattcttg
      361 gctatatgtt tttcaccgtt cccggggcca ttttcaaaga ctcgtcggta agataagatt
      421 gtgtcactcg ctgtctctct tcatttgtcg aagaatgctg aggaatttcg cgatgacgtc
      481 ggcgagtatt ttgaagaatg agaataattt gtatttatac gaaaatcagt tagtggaatt
      541 ttctacaaaa acatgttatc tatagataat tttgttgcaa aatatgttga ctatgacaaa
      601 gattgtatgt atataccttt aatgtattct cattttctta tgtatttata atggcaatga
      661 tgatactgat gatattttaa gatgatgcca gaccacaggc tgatttctgc gtcttttgcc
      721 gaacgcagtg catgtgcggt tgttgttttt tggaatagtt tcaattttcg gactgtccgc
      781 tttgatttca gtttcttggc ttattcaaaa agcaaagtaa agccaaaaaa gcgagatggc
      841 aataccaaat gcggcaaaac ggtagtggaa ggaaaggggt gcggggcagc ggaaggaagg
      901 gtggggcggg gcgtggcggg gtctgtggct gggcgcgacg tcaccgacgt tggagccact
      961 cctttgacca tgtgtgcgtg tgtgtattat tcgtgtctcg ccactcgccg gttgtttttt
     1021 tctttttatc tcgctctctc tagcgccatc tcgtacgcat gctcaacgca ccgcatgttg
     1081 ccgtgtcctt tatgcgtcat tttggctcga aataggcaat tatttaaaca aagattagtc
     1141 aacgaaaacg ctaaaataaa taagtctaca atatggttac ttattgccat gtgtgtgcag
     1201 ccaacgatag caacaaaagc aacaacacag tggctttccc tctttcactt tttgtttgca
     1261 agcgcgtgcg agcaagacgg cacgaccggc aaacgcaatt acgctgacaa agagcagacg
     1321 aagttttggc cgaaaaacat caaggcgcct gatacgaatg catttgcaat aacaattgcg
     1381 atatttaata ttgtttatga agctgtttga cttcaaaaca cacaaaaaaa aaaataaaac
     1441 aaattatttg aaagagaatt aggaatcgga cagcttatcg ttacgggcta acagcacacc
     1501 gagacgaaat agcttacctg acgtcacagc ctctggaaga actgccgcca agcagagaga
     1561 gagagaaaaa gagggagagc agcttagacc gcatgtgctt gtgtgtgagg cgtctctctc
     1621 ttcgtctcct gtttgcgcaa acgcatagac tgcactgaga aaatcgatta cctatttttt
     1681 atgaatgaat atttgcacta ttactattca aaactattaa gatagcaatc acattcaata
     1741 gccaaatact ataccacctg agcgatgcaa cgaaatgatc aatttgagca aaaatgctgc
     1801 atatttagga cggcatcatt atagaaatgc ttcttgctgt gtacttttct ctcgtctggc
     1861 agctgtttcg ccgttattgt taaaaccggc ttaagttagg tgtgttttct acgactagtg
     1921 atgcccctac tagaagatgt gtgttgcaca aatgtccctg aataaccaat ttgaagtgca
     1981 gatagcagta aacgtaagct aatatgaata ttatttaact gtaatgtttt aatatcgctg
     2041 gacattacta ataaacccac tataaacaca tgtacatatg tatgttttgg catacaatga
     2101 gtagttgggg aaaaaatgtg taaaagcacc gtgaccatca cagcataaag ataaccagct
     2161 gaagtatcga atatgagtaa cccccaaatt gaatcacatg ccgcaactga taggacccat
     2221 ggaagtacac tcttcatggc gatatacaag acacacacaa gcacgaacac ccagttgcgg
     2281 aggaaattct ccgtaaatga aaacccaatc ggcgaacaat tcatacccat atatggtaaa
     2341 agttttgaac gcgacttgag agcggagagc attgcggctg ataaggtttt agcgctaagc
     2401 gggctttata aaacgggctg cgggaccagt tttcatatca ctaccgtttg agttcttgtg
     2461 ctgtgtggat actcctcccg acacaaagcc gctccatcag ccagcagtcg tctaatccag
     2521 agaccccgga tcggggtacc aacatggacg cacaaacacg acgacgtgag cgtcgcgctg
     2581 agaaacaagc tcaatggaaa gctgcaaacg gtggatctcc tccacatatg gcttacccat
     2641 acgatgttcc agattacgct cctccaagct tgatgatgac tgaaaagtta aattctgggc
     2701 acactaattt aacaagcaag gggattataa atgatcttca aattgctgga aacacaagtg
     2761 acgatatggg ctggaaatca aagctgaagc tgccgccaaa ggacaaccga ttcaaaacaa
     2821 ctgatgtgac cgatacgcga ggcaatgaat tcgaggagtt ttgccttaaa agagaactgc
     2881 ttatgggtat attcgagaag ggatgggagc gcccttcgcc aattcaggaa gcagctattc
     2941 ctatagcatt aagtggaaaa gatgtgctgg ctcgggccaa gaacggcaca ggcaagacag
     3001 gagcctactg catcccagtt ttagaacaaa tcgacccaac caaggactac attcaggcac
     3061 ttgtgatggt ccccacccga gagttggcgc tacaaacctc acagatatgt attgagctgg
     3121 cgaaacatct tgatatccgg gtaatggtca caacgggagg taccattcta aaggatgaca
     3181 ttcttcgtat ttatcaaaaa gtacaattaa ttattgccac tcccggacga atattagatc
     3241 tgatggataa gaaagttgcc gatatgtcgc attgcagaat attagttttg gatgaggccg
     3301 ataagctatt gtcgcttgat tttcaaggca tgctggatca tgttatacta aagttaccaa
     3361 aggatccaca gatcttgcta ttttccgcaa catttccact aacagtcaag aatttcatgg
     3421 agaagcattt acgcgagcct tacgagatca acctgatgga ggagctaaca ctgaagggtg
     3481 tcactcagta ctatgcattc gtacaggagc gcgcgaaagt agcctgcttg aatgcgctct
     3541 tttcggcgct gcagatcaat cagtctatca tattctgcaa ttccacgcaa cgtgtcgagc
     3601 tattggccaa aaagatcaca gagcttggat attgctgtta ctatatacac gctaagatgg
     3661 cccaggcaca cagaaatcga gtattccacg atttccgcca aggactctgt cgcaatctgg
     3721 tgtgctccga cctgttcacc cgtggtatcg acgtgcaggc cgtgaatgta gtaatcaatt
     3781 tcgatttccc acgaatggca gagacatact tgcatcgaat tggtcgctca ggacgtttcg
     3841 gtcatttggg tattgctatt aatctgataa cctacgagga tcggtttgat ctgcatcgga
     3901 ttgaaaaaga acttggaacc gaaataaagc ctattccgaa ggtcatagac cccgcccttt
     3961 atgtcgcaaa cgttggcgca tccgttggcg atacttgcaa caacagtgat ctgaataatt
     4021 ctgcgaacga ggagggcaac gttagcaaat aagcggccgc tcgagtctag agggcccgcg
     4081 gttcgaaggt aagcctatcc ctaaccctct cctcggtctc gattctacgc gtaccggtca
     4141 tcatcaccat caccattgag tttaaacccg ctgatcagcc tcgactgtgc cttctaaggc
     4201 ctgagctcgc tgatcagcct cgatcgagga tccagacatg ataagataca ttgatgagtt
     4261 tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
     4321 tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat
     4381 tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct
     4441 ctacaaatgt ggtatggctg attatgatca gtcgaccgat gcccttgaga gccttcaacc
     4501 cagtcagctc cttccggtgg gcgcggggca tgactatcgt cgccgcactt atgactgtct
     4561 tctttatcat gcaactcgta ggacaggtgc cggcagcgct ctgggtcatt ttcggcgagg
     4621 accgctttcg ctggagcgcg acgatgatcg gcctgtcgct tgcggtattc ggaatcttgc
     4681 acgccctcgc tcaagccttc gtcactggtc ccgccaccaa acgtttcggc gagaagcagg
     4741 ccattatcgc cggcatggcg gccgacgcgc tgggctacgt cttgctggcg ttcgcgacgc
     4801 gaggctggat ggccttcccc attatgattc ttctcgcttc cggcggcatc gggatgcccg
     4861 cgttgcaggc catgctgtcc aggcaggtag atgacgacca tcagggacag cttcaaggat
     4921 cgctcgcggc tcttaccagc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     4981 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     5041 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     5101 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     5161 gaagcgtggc gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc
     5221 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     5281 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     5341 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     5401 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
     5461 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     5521 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     5581 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     5641 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     5701 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     5761 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     5821 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     5881 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     5941 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     6001 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgctg
     6061 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     6121 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     6181 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     6241 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     6301 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     6361 cacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     6421 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     6481 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     6541 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     6601 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     6661 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     6721 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
     6781 ggcgtatcac gaggcccttt cgtcttcaag gaattaattc tatattctaa aaacacaaat
     6841 gatactt
//
