LOCUS       sequence-288683-        9190 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmSMG6-PINNOB1mut-siRNAres.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9190)
  AUTHORS   Huntzinger E, Kashima I, Fauser M, Sauliere J, Izaurralde E
  TITLE     SMG6 is the catalytic endonuclease that cleaves mRNAs containing 
            nonsense codons in metazoan.
  JOURNAL   RNA. 2008 Dec . 14(12):2609-17.
  PUBMED    18974281
REFERENCE   2  (bases 1 to 9190)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2008 
            Dec . 14(12):2609-17."
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmSMG6-PINNOB1mut-siRNAres_E
FEATURES             Location/Qualifiers
     source          1..9190
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2588..2653
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2678..2704
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6475..6516
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6526..6543
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6566..6583)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6616..6750
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6640..6659)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6694..6713
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(7372..7960)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7452..7471)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8131..8922,8923..8991))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8754..8773
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8992..9096)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9164..9182
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gacgcacaaa cacgacgacg tgagcgtcgc gctgagaaac aagctcaatg
     2641 gaaagctgca aacggtggat ctcctccaca tatggcttac ccatacgatg ttccagatta
     2701 cgctcctcca agcttgaatt ctatgagtcg aaagggaaca cgagaacaaa atgatatatc
     2761 gctgataatt gggacgctgc cgaaacgcct gcagaacaaa atcgcggcca gctgcagcca
     2821 cgcagcagca gcggcaacaa caacaaaggc gttggagacg gaaaacgctg agaatgcgga
     2881 gcccgtggct acgacagcaa atgcttcaag gccagtcgaa atcctgagca atgctagcac
     2941 aggaggaggc aaacccgatg cccagtcaaa gcaacccgtt ttggatacca gcgggaaaat
     3001 ggcgtgggag ggcgggattc caggcatcct gcgcgtgacc acctcggtgg cgggcgatgt
     3061 taacaggaac ggacagggtc agggcaagaa caagagcggt aacagtgctc ggcgcagggg
     3121 tcgcaaggac aaggagcacg ctacgcagtc gccagtgacg cgcacgcgct tgaccaccga
     3181 gcatccggat tggtttgagc cgctgttggg tctgccccat gccaagaata tagtccatct
     3241 gtacgatcag ctcatggtcc tcaatcagag gaatctcata ctgataaact ggaagaactt
     3301 ctgctacatt cacgaccagc tgcaggacgc tttcaagcaa cttcttttgg agcaacttaa
     3361 gttcgtgtgc gagcacaagg ttgatgtctt cttttggaaa ctactcttct acaatgtgcg
     3421 gaattacttg aagcgccagc aaacggacca ggcgcacaca catacgctcc ttctcatcga
     3481 gcaagctatc aagttttacc gcatgctcta cgacaagctg atggccaagt gcgtggcatc
     3541 gtcgcgctgt gacagcgcac ttaaagtggt ggcccagagg ctgctcatct gtttgggcga
     3601 tctgacgcgc taccgggtta accatgtcaa ggccacagat tacatggagg cggccaggta
     3661 ctatcagagg gcgcaggaac tggtacctgg caacggggca cctttcaacc agctggcggt
     3721 catttccatt tatcaccaca agcgcttcga tgcagtttat tactatgtcc gcagcttgtt
     3781 gacttcgaac tctatccaat ctgccaagga aagcctgctg gatttgttcg acgagattcg
     3841 gcgtaagtac gaggagaccg agatgaagca atctcccatg cactatgcag ctggatcgaa
     3901 gaatcaaaag tcgaagcaga tgcgcaagga ggtatggatc tatccggatg ggatcagacg
     3961 cctacatcgc accgatgaca agggcaacaa ggcgaagggc aacaaggcca ctatggcaga
     4021 ggtcaatcgt tacgaggaga tgccgccgga ggagattctt cctcgcattg tttcccttta
     4081 tctgtactta ttaggcaaac tctacaccgg cacagatgtg gactcgttat acccgttgct
     4141 aaggaaactg caaatccaaa taggcgtcgc tttaaggcac gaaaacctgt tgtcacgctc
     4201 caaactgctg aaaatagtag cactcaactt gttcgtcgtc gagcacaaca aacccaaaac
     4261 gtcacgccgc gagatgcgtt atcacagctt caattttgcc aactcattct ttggactcat
     4321 gatgaagaaa acgaaccagc tgctggctgg ttttgtagag gattctacaa atgtccagtg
     4381 tctcccagaa gaagactttg cgactgtgaa cacataccta cagttcgtga atgtttatgt
     4441 acgttggttg agcatcagtt tggacgtgtg ggaacccgtc agatcggaag aacactcttt
     4501 catcgattgc tgggctgagc taacaatcct ttttgagtac attgaatgct taatggggaa
     4561 gcacaaactt gagaaaaacg aaatactgtt ggatgaggat gtggccctta gaggtttcac
     4621 tccgttgggt cgtgaaacgc tgagcatgga ctatctcaaa agaggatccg aacgtcttca
     4681 gtttttcgaa cgtgttcgta ggattggtca gttccaggaa tactacgtcc agcatcaaga
     4741 ggctctccag cagccgttgg aatcaagctt cactgtcgag cacctaaacg cagcgatgaa
     4801 gaacgcattg gaaaactatg atgctgaaag ttgtggtctt gcggcgaacg tttcaaagat
     4861 tcaatgtaaa gccgttgagg aattgttgtg tcgttcaact gattccgatg tttccgagct
     4921 gtgcaagctg aagaaagagc ttgaggccaa ggctagggtt aagcagatat gcaccagcaa
     4981 gttggaggac attctcaagt tcgtggacac gaaaggcggc cgcgccgaaa cgagcgaaaa
     5041 gatcaagttc cttgtggcgg acaccacagc ctttataaac gccgtgcccc tgaatgaata
     5101 cgcggagcag gtgctcaccg tgccggatgt ggtggccgaa gtgcgcaaca agcgccagat
     5161 tcgccgcctc tgcgttctgc ccttcgacct ccaggtgcgc gagcctcgtc cggagagcat
     5221 taaacactgc gtggagttcg ccaagaaaac gggtgactac gcgagtctct cggggatcaa
     5281 tctgaaagtg atctcgctca cctacgaact ggaagcagac aacgtgggca ctaatcacct
     5341 gcgccaggag cctgtgatgg cgcagaccat tgcgtccaag gataaacccg aggagatgca
     5401 ggatgctaat aacaagagac tggtcggttg gtacatgccc gagggcgaag aggaggagga
     5461 ggaggattcc gaggaggaag atgattactc ccaggaagag gacgatgctg aaggcgatga
     5521 gcaggaggca aatgaatccc cagacactgt taaagccgcc atcgaagccc aattggccgg
     5581 caaggaacca tttgccagcg agaccatcga aaaggaggag gaccaagacc aggagcccag
     5641 tcaggaggag ctggacaagc tgttcgagca gctcaaatgc gcacccagtg ccgaggaaga
     5701 gaaagagctc tcggaactgc tcgtttccca gccggccgaa gtagatgagg aggtatccga
     5761 gccacaagaa gccaaacaga gccaaaatct cgacgaggat gtgggtgacg atggctggat
     5821 cacgcattca aacatcaaga aggccaagaa agcgctcgag ggcaaagtgg aaacggatga
     5881 agtgccgccc gtcgcctgca tgaccaccga ttatgcgctg cagaacgtgc tcaagcagct
     5941 aaatctccac ctggccgccc tcaatggccg gatcatcaag cagttgcgca catatatact
     6001 ccgctgctac gcgtgctaca agaccaccag catcatgacc aaggtgtttt gccccaattg
     6061 tggcaacaag acgctgaaac gtgtggccgt cagtctggac gagaacggtc gccaggtgat
     6121 tcacatcaat acgcgccgtc cgctgaccaa taagtacaag aatcagagcc tgcctcgctt
     6181 ccagggcggc aagcattcga gaaatccgat cctgttcgag gatcagccga tgcccaggca
     6241 gatgccgtcg cgcgttgcca agaccaagac gaacgctctg gatcaggact acacggccgg
     6301 attctcgccc ttcgtgctgc gcgacgtgga ctccaagtcc gccatgctgc gctccaaggg
     6361 caacctcaag gagtgggcca ggaataacaa cttcgaggag gatcgccggc gcaagaacta
     6421 caatcgcttg tacaagtagg cggccgctcg agtctagagg gcccgcggtt cgaaggtaag
     6481 cctatcccta accctctcct cggtctcgat tctacgcgta ccggtcatca tcaccatcac
     6541 cattgagttt aaacccgctg atcagcctcg actgtgcctt ctaaggcctg agctcgctga
     6601 tcagcctcga tcgaggatcc agacatgata agatacattg atgagtttgg acaaaccaca
     6661 actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt
     6721 gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt
     6781 caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt
     6841 atggctgatt atgatcagtc gaccgatgcc cttgagagcc ttcaacccag tcagctcctt
     6901 ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg actgtcttct ttatcatgca
     6961 actcgtagga caggtgccgg cagcgctctg ggtcattttc ggcgaggacc gctttcgctg
     7021 gagcgcgacg atgatcggcc tgtcgcttgc ggtattcgga atcttgcacg ccctcgctca
     7081 agccttcgtc actggtcccg ccaccaaacg tttcggcgag aagcaggcca ttatcgccgg
     7141 catggcggcc gacgcgctgg gctacgtctt gctggcgttc gcgacgcgag gctggatggc
     7201 cttccccatt atgattcttc tcgcttccgg cggcatcggg atgcccgcgt tgcaggccat
     7261 gctgtccagg caggtagatg acgaccatca gggacagctt caaggatcgc tcgcggctct
     7321 taccagccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     7381 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     7441 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     7501 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     7561 ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     7621 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     7681 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     7741 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     7801 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     7861 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     7921 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     7981 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     8041 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     8101 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     8161 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     8221 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     8281 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     8341 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     8401 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctgcag gcatcgtggt
     8461 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     8521 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     8581 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     8641 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     8701 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac gggataatac
     8761 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     8821 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     8881 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     8941 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     9001 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     9061 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     9121 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
     9181 gccctttcgt
//
