LOCUS       sequence-288699-        9506 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmGW182-delmf2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9506)
  AUTHORS   Eulalio A, Helms S, Fritzsch C, Fauser M, Izaurralde E
  TITLE     A C-terminal silencing domain in GW182 is essential for miRNA 
            function.
  JOURNAL   RNA. 2009 Jun;15(6):1067-77. doi: 10.1261/rna.1605509. Epub 2009 Apr
            21.
  PUBMED    19383769
REFERENCE   2  (bases 1 to 9506)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.1605509"; journalName: "RNA"; date: "2009-06"; volume: 
            "15"; issue: "6"; pages: "1067-77"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmGW182-delmf2_E
FEATURES             Location/Qualifiers
     source          1..9506
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2589..2654
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2682..2708
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6791..6832
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6842..6859
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6882..6899)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6932..7066
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6956..6975)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7010..7029
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(7688..8276)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7768..7787)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8447..9238,9239..9307))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9070..9089
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9308..9412)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9480..9498
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 tacccaacat ggacgcacaa acacgacgac gtgagcgtcg cgctgagaaa caagctcaat
     2641 ggaaagctgc aaacggtgga tctcctccaa gcttgatggc ttacccatac gatgttccag
     2701 attacgctat gcgtgaagcc cttttttccc aagatggctg gggctgtcag catgttaacc
     2761 aggatactaa ttgggaagtt cccagttcgc cagaaccagc caataaggat gcacccggtc
     2821 caccaatgtg gaagccaagc attaacaatg gtactgatct ttgggagtcc aatttgagaa
     2881 acggaggtca gccggccgca cagcaagttc caaagccgtc gtggggtcat acaccatcct
     2941 ctaacttagg tggaacatgg ggtgaggacg acgatggcgc cgatagtagt agtgtgtgga
     3001 ctggaggagc tgttagcaac gcgggatccg gagctgcagt gggagtaaac caagccggag
     3061 ttaatgtcgg tccaggcggt gttgtttcgt ctggcggacc tcagtgggga caaggtgtcg
     3121 ttggcgtcgg acttggatca actggaggta acgggtcaag caatataact ggatcgtctg
     3181 gagtcgcaac aggtagtagc ggaaactcca gcaacgctgg taacggttgg ggagaccctc
     3241 gtgaaatacg ccctttggga gttggtggct ccatggatat tcgaaatgtt gaacatcgcg
     3301 gcggtaacgg ttctggagca acttcgagcg atccacgaga cattcgcatg atcgatccgc
     3361 gtgaccctat tcgaggagat ccccgtggaa tatctggtcg tcttaatggg acctctgaaa
     3421 tgtggggtca tcatccacaa atgtcccata accagttgca aggtatcaac aaaatggttg
     3481 gtcaaagtgt agcaactgcc agcaccagtg tcggaacatc tggctcgggc atcggtcctg
     3541 gaggtcccgg tcctagtaca gtatcaggca atatcccaac acagtggggg cctgctcaac
     3601 cggtaagcgt tggtgtaagt ggtcccaaag accccgctgc aagcggtcac tggaaagaca
     3661 tgactgattc gataggtcgt agtagtcatc tcatgcgtgg ccaaagccaa acgggaggta
     3721 taggaatagc cggcgttgga aatagcaatg ttccagtggg agccaatcca agtaatccta
     3781 taagcagtgt agttggacct caagcccgga ttccatctgt gggcggcgta caacacaaac
     3841 cagacggcgg cgctatgtgg gtgcattccg gcaatgtagg tggcagaaat aatgttgctg
     3901 ctgttactac ttggggagat gacactcata gcgttaatgt cggcgctccc agcagtggca
     3961 gtgtatccag caacaattgg gttgatgaca agtccaactc aaccttggca caaaactctt
     4021 ggagcgaccc ggcccctgtt ggagttagtt ggggcaataa gcaaagcaaa ccgccaagca
     4081 atagtgcttc atcaggttgg agcactgctg cgggcgtggt ggatggggtt gatctaggat
     4141 ctgagtggaa cacgcacggg gggattattg gaaaatctca gcaacaacaa aaactagcgg
     4201 gacttaacgt gggaatggtg aacgtaatta acgcggagat cattaagcaa agcaagcaat
     4261 acaggatcct tgtcgagaac ggctttaaaa aggaagatgt agagcgggca ttagtgattg
     4321 ctaatatgaa catcgaagag gcagccgata tgctccgtgc caactcatcc ctatcaatgg
     4381 atggttggcg tcgacatgat gagtcccttg gatcttatgc cgaccacaat agttcaacaa
     4441 gcagcggtgg atttgctggt cgttacccgg tcaacagtgg acaaccttca atgtcctttc
     4501 ctcataataa ccttatgaat aacatgggag gtaccgctgt tactggaggt aacaacaata
     4561 caaacatgac agctttacag gtgcaaaagt atttaaatca agggcaacat ggtgtcgctg
     4621 ttggaccgca agccgttggt aattcttcag cagtatctgt cggatttggt cagaacacgt
     4681 ctaacgcagc agtggcagga gcagcctctg taaatatagc agcaaataca aacaaccaac
     4741 cgtctggtca gcaaattcgc atgctaggcc agcaaattca gttggccatt catagtggtt
     4801 tcatatctag tcagatattg actcaaccgc taactcaaac aacccttaac cttttaaacc
     4861 aacttcttag caatattaag catctccagg ctgcgcagca atcccttacc cgcgggggaa
     4921 atgtcaatcc aatggcagtg aatgtggcta tatctaaata caagcagcaa atccagaatt
     4981 tacagaacca gataaatgca caacaggctg tgtatgtaaa acagcaaaat atgcaaccaa
     5041 cttcacaaca acaacagccc caacaacagc aacttccttc tgttcatcta agtaactcag
     5101 gcaacgacta tttaagaggt cacgatgcaa taaataattt gcaaagcaac ttttctgagc
     5161 tcaatattaa taagccaagt ggatatcaag gagcgtccaa tcaacaatcc cgattaaatc
     5221 agtggaagct tccagtatta gataaggaga tcaactctga cagtacggaa ttttctcgtg
     5281 ccccaggtgc aacgaaacaa aatttgacgg ccaacacaag caacataaac tctttgggtc
     5341 ttcaaaacga tagtacatgg tcaactggac gcagtattgg tgacggttgg cctgatccct
     5401 catctgataa cgagaataaa gactggtctg ttgctcagcc aacttcagca gcaactgctt
     5461 acactgatct ggtccaagag tttgagccag gcaagccatg gaagggttca cagatcaaaa
     5521 gcatagaaga tgatcccagc attacaccag gaagcgttgc tagatctcca ttgtctatta
     5581 attcgacgcc aaaagatgct gacatatttg ccaataccgg taaaaattca ccgactgatt
     5641 taccgccact aagtttatcg tcgtctacat ggagttttaa tccaaaccaa aattatccga
     5701 gtcacagttg gtctgacaat agtcaacaat gtaccgccac ttcggagctt tggacaagcc
     5761 cgctaaataa atcatcgtct cgaggtcccc cgccaggatt gactgccaat tcaaataaat
     5821 ctgcaaatag taatgcgtca acgccaacaa ctattaccgg aggtgcgaat ggatggttac
     5881 agcctcgaag tggcggtgtt caaaccacaa acactaattg gacaggtggt aacaccactt
     5941 ggggctccag ttggttgctt ttgaaaaatc taacagcaca gattgatggt cctactttgc
     6001 gtacactgtg tatgcagcat gggccccttg tcagctttca cccgtatttg aaccaaggaa
     6061 ttgccttatg taaatatact actcgtgagg aggcgaacaa ggcgcaaatg gcgttaaaca
     6121 actgtgtcct cgccaacacc acaatatttg ctgaatctcc cagcgagaac gaggtgcaaa
     6181 gcattatgca gcacttacca caaactcctt cctctacaag ctctagtgga actagtggtg
     6241 gcaacgtcgg aggcgtcggc acttcagcca ataatgcaaa cagtggttct gcagcttgtc
     6301 tgtccggaaa caatagcggc aacggaaacg gcagcgcgag cggcgccggc agcggcaaca
     6361 atggcaacag tagctgcaac aacagtgccg ccgggggggg cagcagcagc aacaacacga
     6421 ttaccactgt agcaaattcg aatcttgttg gttctagtgg ctctgtctca aattcctctg
     6481 gcgttactgc taactctagt actgtttctg tagttagttg tacagcgagt gggaattcca
     6541 taaatggggc aggtactgca aacagttctg gttcaaagag tagtgcaaac aatttagcta
     6601 gcggccagtc tagcgcttct aacttaacta atagcaccaa ttcaacatgg cgacaaacta
     6661 gccaaaacca agctcttcaa agtcaaagca ggccatcagg cagagaagct gactttgatt
     6721 atatatctct cgtttattcc attgttgatg attaagcggc cgctcgagtc tagagggccc
     6781 gcggttcgaa ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgcgtaccgg
     6841 tcatcatcac catcaccatt gagtttaaac ccgctgatca gcctcgactg tgccttctaa
     6901 ggcctgagct cgctgatcag cctcgatcga ggatccagac atgataagat acattgatga
     6961 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
     7021 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
     7081 cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa
     7141 cctctacaaa tgtggtatgg ctgattatga tcagtcgacc gatgcccttg agagccttca
     7201 acccagtcag ctccttccgg tgggcgcggg gcatgactat cgtcgccgca cttatgactg
     7261 tcttctttat catgcaactc gtaggacagg tgccggcagc gctctgggtc attttcggcg
     7321 aggaccgctt tcgctggagc gcgacgatga tcggcctgtc gcttgcggta ttcggaatct
     7381 tgcacgccct cgctcaagcc ttcgtcactg gtcccgccac caaacgtttc ggcgagaagc
     7441 aggccattat cgccggcatg gcggccgacg cgctgggcta cgtcttgctg gcgttcgcga
     7501 cgcgaggctg gatggccttc cccattatga ttcttctcgc ttccggcggc atcgggatgc
     7561 ccgcgttgca ggccatgctg tccaggcagg tagatgacga ccatcaggga cagcttcaag
     7621 gatcgctcgc ggctcttacc agccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     7681 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     7741 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     7801 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     7861 cgggaagcgt ggcgctttct caatgctcac gctgtaggta tctcagttcg gtgtaggtcg
     7921 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     7981 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     8041 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     8101 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
     8161 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     8221 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     8281 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     8341 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     8401 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     8461 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     8521 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     8581 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     8641 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     8701 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     8761 ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     8821 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     8881 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     8941 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     9001 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     9061 caacacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     9121 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     9181 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     9241 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     9301 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     9361 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     9421 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
     9481 ataggcgtat cacgaggccc tttcgt
//
