LOCUS       sequence-288704-        7795 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of HsTNRC6C_Cterm.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7795)
  TITLE     Izaurralde lab plasmids
REFERENCE   2  (bases 1 to 7795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-HsTNRC6C_C-term_F
FEATURES             Location/Qualifiers
     source          1..7795
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2588..2653
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2678..2704
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5080..5121
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5131..5148
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5171..5188)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5221..5355
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5245..5264)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5299..5318
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(5977..6565)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6057..6076)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6736..7527,7528..7596))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7359..7378
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7597..7701)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7769..7787
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gacgcacaaa cacgacgacg tgagcgtcgc gctgagaaac aagctcaatg
     2641 gaaagctgca aacggtggat ctcctccaca tatggcttac ccatacgatg ttccagatta
     2701 cgctcctcca agcttgaatt ctgcagatat cgcccccaaa aaaggacttc aaaagggcat
     2761 gaagacgtct ggcaagcagg atgaggcctg gatcatgagc cggctgatca aacaactcac
     2821 agacatgggc ttcccgagag agccagctga ggaggccttg aagagtaaca atatgaatct
     2881 tgatcaggcc atgagcgctc tgctggaaaa gaaggtggac gtggacaagc gtgggctggg
     2941 agtgaccgac cataatggaa tggccgccaa gcccctcggc tgccgcccgc caatctccaa
     3001 agagtcttcc gtggaccgcc ccacctttct tgacaaggat ggcggcctcg tggaagagcc
     3061 cacgccttca ccgttcttgc cttccccaag cctgaagctc cccctttcac acagtgcact
     3121 ccccagtcag gccctgggtg ggattgcctc cgggctgggc atgcaaaact tgaattcttc
     3181 tagacagata ccgagtggca atctgggtat gtttggcaat agtggagcag cacaagccag
     3241 gaccatgcag cagccgccac agccaccagt gcagcctctt aactcttccc agcccagtct
     3301 ccgtgctcaa gtgcctcagt ttctatcccc tcaggttcaa gcacagcttt tgcagtttgc
     3361 agcaaaaaac attggtctca accctgcact attaacctcg ccaattaatc ctcaacatat
     3421 gacgatgttg aaccagctct atcagctgca gctggcatac caacgtttac aaatccagca
     3481 gcagatgtta caggcccagc gtaatgtgtc cggatccatg agacaacagg agcagcaagt
     3541 tgcgcgcaca atcactaatc tgcagcagca gatccagcag caccagcgcc agctggccca
     3601 ggccctgctc gtgaagcagc caccaccgcc accgcccccg ccgcacctgt ctctgcaccc
     3661 ctctgcaggc aaatcggcca tggacagctt cccctcgcac ccacagactc ccggcctacc
     3721 tgacctgcag accaaagagc agcagtcttc acccaacacc tttgctcctt accctctcgc
     3781 tggactgaac ccaaacatga atgtcaacag catggacatg accggtggct tgtcggtgaa
     3841 ggacccatcc cagtcccagt cacgcctccc ccagtggacg caccccaact ccatggataa
     3901 cttgcccagt gccgcttccc ccctggagca gaaccctagc aagcatggtg ctatccctgg
     3961 aggtctaagc attgggcctc caggtaagtc ctccattgat gactcctatg gccggtacga
     4021 tttaatccag aacagtgagt caccagccag tcctcccgta gctgttcccc atagctggtc
     4081 acgtgccaaa tctgacagtg ataaaatctc aaatggctct agcatcaact ggcccccaga
     4141 attccatccg ggagttccat ggaaaggact gcagaatatt gaccctgaga atgaccctga
     4201 cgtcactcct ggcagtgtcc ccactgggcc taccatcaac accaccatcc aggatgtcaa
     4261 ccgctacctc ctcaagagtg gaggtaaact gtcagacatc aaatcgacgt ggtcctctgg
     4321 ccctacctcc cacacgcaag cctctctgtc tcatgaacta tggaaggtgc ccagaaacag
     4381 tactgcaccc acgaggccac ctccagggtt aaccaatccc aagccctcct ccacctgggg
     4441 tgccagcccc ctcggctgga ccagctccta ctcctcgggt tctgcctgga gcaccgacac
     4501 ctcaggaaga accagcagct ggctcgttct tcgaaacctc actccccaga ttgatggttc
     4561 tacactgcgg acattgtgtt tgcaacatgg gcctcttatc acattccacc tgaatctgac
     4621 tcaaggcaat gctgtggtcc ggtacagctc caaggaggag gctgccaagg cccagaagtc
     4681 tctgcacatg tgcgtcctgg gaaacactac catcctggcc gagttcgctg gtgaagaaga
     4741 agtgaatcgc ttcttagccc aaggccaggc gctgccaccc acttccagct ggcagtccag
     4801 cagcgcgtcc agccagccgc ggctcagcgc agcgggcagc tcccatggcc tggtacgcag
     4861 cgacgctggc cactggaacg ccccgtgcct gggtggcaag gggagcagtg agctgctgtg
     4921 gggcggggtg ccccagtact ccagcagcct gtggggcccg cccagcgccg acgacagcag
     4981 ggtgataggc agccccacgc cgctaaccac cctgctgcct ggggacctgc tcagcgggga
     5041 gtccctgtag actcgagtct agagggcccg cggttcgaag gtaagcctat ccctaaccct
     5101 ctcctcggtc tcgattctac gcgtaccggt catcatcacc atcaccattg agtttaaacc
     5161 cgctgatcag cctcgactgt gccttctaag gcctgagctc gctgatcagc ctcgatcgag
     5221 gatccagaca tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga
     5281 aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc
     5341 tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag
     5401 gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtatggc tgattatgat
     5461 cagtcgaccg atgcccttga gagccttcaa cccagtcagc tccttccggt gggcgcgggg
     5521 catgactatc gtcgccgcac ttatgactgt cttctttatc atgcaactcg taggacaggt
     5581 gccggcagcg ctctgggtca ttttcggcga ggaccgcttt cgctggagcg cgacgatgat
     5641 cggcctgtcg cttgcggtat tcggaatctt gcacgccctc gctcaagcct tcgtcactgg
     5701 tcccgccacc aaacgtttcg gcgagaagca ggccattatc gccggcatgg cggccgacgc
     5761 gctgggctac gtcttgctgg cgttcgcgac gcgaggctgg atggccttcc ccattatgat
     5821 tcttctcgct tccggcggca tcgggatgcc cgcgttgcag gccatgctgt ccaggcaggt
     5881 agatgacgac catcagggac agcttcaagg atcgctcgcg gctcttacca gccagcaaaa
     5941 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     6001 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     6061 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     6121 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg
     6181 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     6241 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     6301 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     6361 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
     6421 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     6481 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     6541 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     6601 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     6661 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     6721 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     6781 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     6841 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     6901 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     6961 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     7021 taatagtttg cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt
     7081 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     7141 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     7201 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     7261 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     7321 gcggcgaccg agttgctctt gcccggcgtc aacacgggat aataccgcgc cacatagcag
     7381 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     7441 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     7501 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     7561 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     7621 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     7681 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
     7741 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgt
//
