LOCUS       sequence-288751-        7963 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of HsTNRC6C-Cterm.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7963)
  AUTHORS   Lazzaretti D, Tournier I, Izaurralde E
  TITLE     The C-terminal domains of human TNRC6A, TNRC6B, and TNRC6C silence 
            bound transcripts independently of Argonaute proteins.
  JOURNAL   RNA. 2009 Jun . 15(6):1059-66.
  PUBMED    19383768
REFERENCE   2  (bases 1 to 7963)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA. 2009 
            Jun . 15(6):1059-66."
COMMENT     Sequence Label: pCIneo-lambdaN-HA-HsTNRC6C-C-term_F
FEATURES             Location/Qualifiers
     source          1..7963
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..729
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     680..700
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     726..750
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          890..1022
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1005..1024
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1067..1085
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1089..1098
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1095..1160
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             1197..1223
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1203..1229
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     polyA_signal    3667..3788
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3678..3697)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3732..3751
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      3974..4429
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4061..4080)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4271..4292
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4546..4903
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4546..4737
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4569..4589)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4754..4889
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4954..5748
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5008..5027)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5618..5637
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5812..5860
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(5901..5920)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6020..6042
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6080..6098)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6166..6270
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6271..6339,6340..7131)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6489..6508)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7302..7890
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7791..7810
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactg
      661 cgatcgcccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
      721 agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac
      781 agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaactt aagctgcagt
      841 gactctctta aggtagcctt gcagaagttg gtcgtgaggc actgggcagg taagtatcaa
      901 ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac agagaagact
      961 cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac
     1021 aggtgtccac tcccagttca attacagctc ttaaggctag agtacttaat acgactcact
     1081 ataggctagc caccatggac gcacaaacac gacgacgtga gcgtcgcgct gagaaacaag
     1141 ctcaatggaa agctgcaaac ccaccgctcg aggctgccca agcttacacc atggcctacc
     1201 catatgatgt tccagattac gcttcactcg acgcggatcc cgtcgaattc acgcgtggta
     1261 cctctagagt cgacgccccc aaaaaaggac ttcaaaaggg catgaagacg tctggcaagc
     1321 aggatgaggc ctggatcatg agccggctga tcaaacaact cacagacatg ggcttcccga
     1381 gagagccagc tgaggaggcc ttgaagagta acaatatgaa tcttgatcag gccatgagcg
     1441 ctctgctgga aaagaaggtg gacgtggaca agcgtgggct gggagtgacc gaccataatg
     1501 gaatggccgc caagcccctc ggctgccgcc cgccaatctc caaagagtct tccgtggacc
     1561 gccccacctt tcttgacaag gatggcggcc tcgtggaaga gcccacgcct tcaccgttct
     1621 tgccttcccc aagcctgaag ctcccccttt cacacagtgc actccccagt caggccctgg
     1681 gtgggattgc ctccgggctg ggcatgcaaa acttgaattc ttctagacag ataccgagtg
     1741 gcaatctggg tatgtttggc aatagtggag cagcacaagc caggaccatg cagcagccgc
     1801 cacagccacc agtgcagcct cttaactctt cccagcccag tctccgtgct caagtgcctc
     1861 agtttctatc ccctcaggtt caagcacagc ttttgcagtt tgcagcaaaa aacattggtc
     1921 tcaaccctgc actattaacc tcgccaatta atcctcaaca tatgacgatg ttgaaccagc
     1981 tctatcagct gcagctggca taccaacgtt tacaaatcca gcagcagatg ttacaggccc
     2041 agcgtaatgt gtccggatcc atgagacaac aggagcagca agttgcgcgc acaatcacta
     2101 atctgcagca gcagatccag cagcaccagc gccagctggc ccaggccctg ctcgtgaagc
     2161 agccaccacc gccaccgccc ccgccgcacc tgtctctgca cccctctgca ggcaaatcgg
     2221 ccatggacag cttcccctcg cacccacaga ctcccggcct acctgacctg cagaccaaag
     2281 agcagcagtc ttcacccaac acctttgctc cttaccctct cgctggactg aacccaaaca
     2341 tgaatgtcaa cagcatggac atgaccggtg gcttgtcggt gaaggaccca tcccagtccc
     2401 agtcacgcct cccccagtgg acgcacccca actccatgga taacttgccc agtgccgctt
     2461 cccccctgga gcagaaccct agcaagcatg gtgctatccc tggaggtcta agcattgggc
     2521 ctccaggtaa gtcctccatt gatgactcct atggccggta cgatttaatc cagaacagtg
     2581 agtcaccagc cagtcctccc gtagctgttc cccatagctg gtcacgtgcc aaatctgaca
     2641 gtgataaaat ctcaaatggc tctagcatca actggccccc agaattccat ccgggagttc
     2701 catggaaagg actgcagaat attgaccctg agaatgaccc tgacgtcact cctggcagtg
     2761 tccccactgg gcctaccatc aacaccacca tccaggatgt caaccgctac ctcctcaaga
     2821 gtggaggtaa actgtcagac atcaaatcga cgtggtcctc tggccctacc tcccacacgc
     2881 aagcctctct gtctcatgaa ctatggaagg tgcccagaaa cagtactgca cccacgaggc
     2941 cacctccagg gttaaccaat cccaagccct cctccacctg gggtgccagc cccctcggct
     3001 ggaccagctc ctactcctcg ggttctgcct ggagcaccga cacctcagga agaaccagca
     3061 gctggctcgt tcttcgaaac ctcactcccc agattgatgg ttctacactg cggacattgt
     3121 gtttgcaaca tgggcctctt atcacattcc acctgaatct gactcaaggc aatgctgtgg
     3181 tccggtacag ctccaaggag gaggctgcca aggcccagaa gtctctgcac atgtgcgtcc
     3241 tgggaaacac taccatcctg gccgagttcg ctggtgaaga agaagtgaat cgcttcttag
     3301 cccaaggcca ggcgctgcca cccacttcca gctggcagtc cagcagcgcg tccagccagc
     3361 cgcggctcag cgcagcgggc agctcccatg gcctggtacg cagcgacgct ggccactgga
     3421 acgccccgtg cctgggtggc aaggggagca gtgagctgct gtggggcggg gtgccccagt
     3481 actccagcag cctgtggggc ccgcccagcg ccgacgacag cagggtgata ggcagcccca
     3541 cgccgctaac caccctgctg cctggggacc tgctcagcgg ggagtccctg taggcgctgc
     3601 catcatcagc accaggagag gcggccgctt ccctttagtg agggttaatg cttcgagcag
     3661 acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat
     3721 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
     3781 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gagatgtggg
     3841 aggtttttta aagcaagtaa aacctctaca aatgtggtaa aatccgataa ggatcgatcc
     3901 gggctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
     3961 gaatggcgaa tggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     4021 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     4081 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     4141 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     4201 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     4261 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     4321 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     4381 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc ctgatgcggt
     4441 attttctcct tacgcatctg tgcggtattt cacaccgcat acgcggatct gcgcagcacc
     4501 atggcctgaa ataacctctg aaagaggaac ttggttaggt accttctgag gcggaaagaa
     4561 ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
     4621 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     4681 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
     4741 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     4801 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     4861 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagcttg attcttctga
     4921 cacaacagtc tcgaacttaa ggctagagcc accatgattg aacaagatgg attgcacgca
     4981 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
     5041 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc
     5101 aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg
     5161 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg
     5221 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct
     5281 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct
     5341 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa
     5401 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa
     5461 ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc
     5521 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
     5581 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
     5641 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc
     5701 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg
     5761 ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgatggccg caataaaata
     5821 tctttatttt cattacatct gtgtgttggt tttttgtgtg aatcgatagc gataaggatc
     5881 cgcgtatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga
     5941 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     6001 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     6061 aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     6121 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     6181 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     6241 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     6301 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     6361 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     6421 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     6481 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     6541 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     6601 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     6661 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     6721 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     6781 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     6841 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     6901 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     6961 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     7021 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     7081 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     7141 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     7201 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     7261 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     7321 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     7381 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     7441 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     7501 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     7561 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     7621 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     7681 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     7741 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     7801 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     7861 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     7921 ctggcctttt gctggccttt tgctcacatg gctcgacaga tct
//
