LOCUS       sequence-28884-e         709 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Warthmann N, Chen H, Ossowski S, Weigel D, Herve P
  TITLE     Highly specific gene silencing by artificial miRNAs in rice.
  JOURNAL   PLoS One. 2008  . 3(3):e1829.
  PUBMED    18350165
REFERENCE   2  (bases 1 to 709)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2008  . 3(3):e1829."
COMMENT     Sequence Label: pNW55
FEATURES             Location/Qualifiers
     source          1..709
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     8..24
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8..24
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(319..335)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(320..336)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(364..382)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(403..419)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(403..419)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(416..438)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    427..443
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(451..457,458..475,476..481))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    496..517
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(634..651)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cggccgctct agaactagtg gatccccctc ggatcccagc agcagccaca gcaaaatttg
       61 gtttgggata ggtaggtgtt atgttaggtc tggttttttg gctgtagcag cagcagtgga
      121 aggggcatgc agaggagcag gagattcagt ttgaagctgg acttcacttt tgcctctctc
      181 tcctgtgctt gcctcttcca ttcctgctgc taggctgttc tgtggaagtt tgcagagttt
      241 atattatggg tttaatcgtc catggcatca gcatcagcag cggtaccgag ggctgcagga
      301 attcgatatc aagcttatcg ataccgtcga cctcgagggg gggcccggta ccagcttttg
      361 ttccctttag tgagggttaa tttcgagctt ggcgtaatca tggtcatagc tgtttcctgt
      421 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
      481 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
      541 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
      601 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
      661 cgttcggctg cggcgagcgg tatcagctca ctcaaggcgg taatacggt
//
