LOCUS       sequence-28897-e         745 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Fuentealba LC, Eivers E, Ikeda A, Hurtado C, Kuroda H, Pera EM, De 
            Robertis EM
  TITLE     Integrating patterning signals: Wnt/GSK3 regulates the duration of 
            the BMP/Smad1 signal.
  JOURNAL   Cell. 2007 Nov 30. 131(5):980-93.
  PUBMED    18045539
REFERENCE   2  (bases 1 to 745)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2007 
            Nov 30. 131(5):980-93."
COMMENT     Sequence Label: pCS2 hSmad1-EVE-MM
FEATURES             Location/Qualifiers
     source          1..745
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    394..528
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(418..437)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     472..491
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(640..660)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(640..658)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(679..695)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(679..695)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(692..714)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    703..719
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 gtcggactgc aactaccatc atggatttca tcctactact gtttgcaaga tccctagtgg
       61 gtgtagtctg aaaattttta acaaccaaga atttgctcag ttattggcac agtctgtgaa
      121 ccatggattt gagacagtct atgagcttac aaaaatgtgt actatacgta tgagctttgt
      181 gaagggctgg ggagcagaat accaccgcca ggatgttact agcaccccct gctggattga
      241 gatacatctg cacggccccc tccagtggct ggataaagtt cttactcaaa tgggttcacc
      301 tcataatcct atttcagaag tagaataagg atcccatcga ttcgaattca aggcctctcg
      361 agcctctaga actatagtga gtcgtattac gtagatccag acatgataag atacattgat
      421 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt
      481 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat
      541 tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta attcgcggcc
      601 gcggcgccnt gcattgggcc cggtacccag cttttgttcc ctttagtgag ggttaattgc
      661 gcgcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
      721 tccacacaac atacgagccg ggagc
//
