LOCUS       sequence-289043-       10654 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of HsTNRC6C-delDUF-siRNAres.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10654)
  AUTHORS   Eulalio A, Helms S, Fritzsch C, Fauser M, Izaurralde E
  TITLE     A C-terminal silencing domain in GW182 is essential for miRNA 
            function.
  JOURNAL   RNA. 2009 Jun;15(6):1067-77. doi: 10.1261/rna.1605509. Epub 2009 Apr
            21.
  PUBMED    19383769
REFERENCE   2  (bases 1 to 10654)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.1605509"; journalName: "RNA"; date: "2009-06"; volume: 
            "15"; issue: "6"; pages: "1067-77"
COMMENT     Sequence Label: pCIneo-lambdaN-HA-HsTNRC6C-delDUF-siRNAres_I
FEATURES             Location/Qualifiers
     source          1..10654
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..729
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     680..700
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     726..750
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          890..1022
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1005..1024
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        1067..1085
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1089..1098
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1095..1160
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             1197..1223
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1203..1229
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             3530..3547
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    6358..6479
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6369..6388)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6423..6442
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      6665..7120
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6752..6771)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6962..6983
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7237..7594
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7237..7428
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7260..7280)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7445..7580
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7645..8439
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7699..7718)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8309..8328
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8503..8551
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(8592..8611)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8711..8733
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8771..8789)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8857..8961
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8962..9030,9031..9822)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9180..9199)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9993..10581
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10482..10501
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactg
      661 cgatcgcccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
      721 agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac
      781 agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaactt aagctgcagt
      841 gactctctta aggtagcctt gcagaagttg gtcgtgaggc actgggcagg taagtatcaa
      901 ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac agagaagact
      961 cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac
     1021 aggtgtccac tcccagttca attacagctc ttaaggctag agtacttaat acgactcact
     1081 ataggctagc caccatggac gcacaaacac gacgacgtga gcgtcgcgct gagaaacaag
     1141 ctcaatggaa agctgcaaac ccaccgctcg aggctgccca agcttacacc atggcctacc
     1201 catatgatgt tccagattac gcttcactcg acgcggatcc cgtcgaattc atggctacag
     1261 ggagtgccca gggcaacttc actggacata ccaagaagac aaatggcaat aatggcacca
     1321 atggcgcact cgtccaaagc ccttctaatc agagtgccct tggagcaggg ggagcgaaca
     1381 gtaatggaag tgcggccaga gtgtggggtg tagccacagg ctccagctct ggcctggctc
     1441 actgctctgt cagtggtggg gatggaaaaa tggacactat gattggagat gggagaagtc
     1501 agaattgctg gggtgcttcc aactccaatg ctggcattaa tcttaacctt aatcctaatg
     1561 ccaacccagc tgcctggcct gtacttggac atgaaggaac cgtggcgaca ggcaaccctt
     1621 ccagtatttg cagtccagtc agtgccatag gtcaaaatat gggcaaccag aacgggaacc
     1681 caacaggcac tttaggtgct tggggaaact tgctgccaca agagagcaca gaaccacaaa
     1741 cgtccacttc tcagaatgtg tctttcagcg cacaacctca gaaccttaac actgatggac
     1801 caaataacac taaccccatg aactcttcac ccaaccctat caatgcaatg cagacaaatg
     1861 gactgccaaa ctggggcatg gctgttggta tgggggccat catcccgccc cacctgcaag
     1921 gccttcctgg tgctaatgga tcatcagttt ctcaagtcag tgggggcagt gctgaaggaa
     1981 taagcaattc tgtgtgggga ctgtccccag gtaaccctgc cacaggaaat agcaattctg
     2041 ggttcagtca ggggaatgga gacactgtga actcagcttt gagcgccaag cagaatggat
     2101 ccagcagtgc tgtgcaaaag gaaggaagtg gaggaaatgc ttgggattca ggacctcctg
     2161 ctggtcctgg aatactcgcc tggggaaggg gcagtggcaa caatggcgtt ggtaatatcc
     2221 attcaggagc ttggggccac cccagccgaa gcacctctaa cggtgtgaat ggggaatggg
     2281 gaaagccccc aaaccagcat tccaacagtg acatcaatgg gaaaggatca acagggtggg
     2341 agagtcctag tgtcaccagc cagaacccta ccgtacagcc tggtggtgaa cacatgaact
     2401 cctgggccaa agcggcatct tctggaacta cagcaagtga aggaagtagt gatggttctg
     2461 gcaaccacaa tgaaggaagc actgggaggg aaggaacggg agaaggccga aggcgagata
     2521 aagggattat agaccaaggg cacatccagt tgccaaggaa tgatcttgac cctagggtcc
     2581 tctccaacac tggttgggga cagactcctg taaagcaaaa cactgcctgg gaatttgaag
     2641 aatcccctag gtctgaaagg aaaaatgaca atgggacaga ggcctggggt tgtgcagcta
     2701 ctcaggcttc aaactcaggg gggaagaacg atgggtccat catgaacagt acaaatacct
     2761 cttcagtatc tgggtgggtc aacgcgccac ctgccgctgt gccagcaaac acaggttggg
     2821 gagacagcaa caacaaagcg ccaagtggcc cgggggtttg gggggactcg ataagctcta
     2881 ctgctgttag tactgctgct gctgccaaga gtggccatgc ttggagtggg gccgcaaatc
     2941 aggaggacaa gtcacccacc tggggtgagc ctccaaagcc caaatcccaa cactggggag
     3001 atggacaaag atcaaatcca gcctggagtg caggaggggg agattgggca gattcatcgt
     3061 ctgtccttgg acacttgggg gatgggaaaa aaaatggatc tggatgggat gctgacagta
     3121 ataggtcagg gtctggttgg aatgacacca cgagatctgg gaacagtggc tggggcaaca
     3181 gcacaaatac aaaggccaat ccaggtacaa actgggggga gactttaaaa cctggccccc
     3241 aacagaactg ggctagcaaa ccccaagaca acaatgtgag taactgggga ggagctgctt
     3301 ctgtgaaaca gacaggaaca gggtggatcg gggggccggt accggtcaaa cagaaggaca
     3361 gcagtgaagc aactggctgg gaagaaccct ctccaccgtc cattcgccgc aaaatggaaa
     3421 ttgatgatgg tacctcagct tggggggacc caagcaacta taacaataaa actgtaaaca
     3481 tgtgggatag aaacaacccg gtcatccaga gcagtaccac gaccaatacc accaccacca
     3541 ccaccactac cacgagcaac accacacaca gggtcgagac gccgcccccg caccaggctg
     3601 gtactcagct gaatcgatca ccgttgcttg gtccaggtag gaaagtttca tcaggctggg
     3661 gagaaatgcc taatgttcac tcaaagactg aaaactcttg gggagaacca tcctcccctt
     3721 ctaccctggt ggataatggc acagcagcat gggggaagcc acccagcagt ggcagcgggt
     3781 ggggagatca ccctgccgag ccgccggtgg catttggaag agctggcgca cctgttgctg
     3841 cctcagccct gtgcaaacca gcttcaaaat ctatgcaaga aggctggggc agtggtgggg
     3901 atgaaatgaa cctcagtacc agccagtggg aggatgaaga aggggacgtg tggaataatg
     3961 ctgcttccca agaaagcacc tcctcctgca gctcctgggg gaacgccccc aaaaaaggac
     4021 ttcaaaaggg catgaagacg tctggcaagc aggatgaggc ctggatcatg agccggctga
     4081 tcaaacaact cacagacatg ggcttcccga gagagccagc tgaggaggcc ttgaagagta
     4141 acaatatgaa tcttgatcag gccatgagcg ctctgctgga aaagaaggtg gacgtggaca
     4201 agcgtgggct gggagtgacc gaccataatg gaatggccgc caagcccctc ggctgccgcc
     4261 cgccaatctc caaagagtct tccgtggacc gccccacctt tcttgacaag gatggcggcc
     4321 tcgtggaaga gcccacgcct tcaccgttct tgccttcccc aagcctgaag ctcccccttt
     4381 cacacagtgc actccccagt caggccctgg gtgggattgc ctccgggctg ggcatgcaaa
     4441 acttgaattc ttctagacag ataccgagtg gcaatctggg tatgtttggc aatagtggag
     4501 cagcacaagc caggaccatg cagcagccgc cacagccacc agtgcagcct cttaactctt
     4561 cccagcccag tctccgtgct caagtgcctc agtttctatc ccctcaggtt caagcacagc
     4621 ttttgcagtt tgcagcaaaa aacattggtc tcaaccctgc actattaacc tcgccaatta
     4681 atcctcaaca tatgacgatg ttgaaccagc tctatcagct gcagctggca taccaacgtt
     4741 tacaaatcca gcagcagatg ttacaggccc agcgtaatgt gtccggatcc atgagacaac
     4801 aggagcagca agttgcgcgc acaatcacta atctgcagca gcagatccag cagcaccagc
     4861 gccagctggc ccaggccctg ctcgtgaagc agccaccacc gccaccgccc ccgccgcacc
     4921 tgtctctgca cccctctgca ggcaaatcgg ccatggacag cttcccctcg cacccacaga
     4981 ctcccggcct acctgacctg cagaccaaag agcagcagtc ttcacccaac acctttgctc
     5041 cttaccctct cgctggactg aacccaaaca tgaatgtcaa cagcatggac atgaccggtg
     5101 gcttgtcggt gaaggaccca tcccagtccc agtcacgcct cccccagtgg acgcacccca
     5161 actccatgga taacttgccc agtgccgctt cccccctgga gcagaaccct agcaagcatg
     5221 gtgctatccc tggaggtcta agcattgggc ctccaggtaa gtcctccatt gatgactcct
     5281 atggccggta cgatttaatc cagaacagtg agtcaccagc cagtcctccc gtagctgttc
     5341 cccatagctg gtcacgtgcc aaatctgaca gtgataaaat ctcaaatggc gtccccactg
     5401 ggcctaccat caacaccacc atccaggatg tcaaccgcta cctcctcaag agtggaggta
     5461 aactgtcaga catcaaatcg acgtggtcct ctggccctac ctcccacacg caagcctctc
     5521 tgtctcatga actatggaag gtgcccagaa acagtactgc acccacgagg ccacctccag
     5581 ggttaaccaa tcccaagccc tcctccacct ggggtgccag ccccctcggc tggaccagct
     5641 cctactcctc gggttctgcc tggagcaccg acacctcagg aagaaccagc agctggctcg
     5701 ttcttcgaaa cctcactccc cagattgatg gttctacact gcggacattg tgtttgcaac
     5761 atgggcctct tatcacattc cacctgaatc tgactcaagg caatgctgtg gtccggtaca
     5821 gctccaagga ggaggctgcc aaggcccaga agtctctgca catgtgcgtc ctgggaaaca
     5881 ctaccatcct ggccgagttc gctggtgaag aagaagtgaa tcgcttctta gcccaaggcc
     5941 aggcgctgcc acccacttcc agctggcagt ccagcagcgc gtccagccag ccgcggctca
     6001 gcgcagcggg cagctcccat ggcctggtac gcagcgacgc tggccactgg aacgccccgt
     6061 gcctgggtgg caaggggagc agtgagctgc tgtggggcgg ggtgccccag tactccagca
     6121 gcctgtgggg cccgcccagc gccgacgaca gcagggtgat aggcagcccc acgccgctaa
     6181 ccaccctgct gcctggggac ctgctcagcg gggagtccct gtaggcgctg ccatcatcag
     6241 caccaggaga gccgacccct cccgggaccc ctcccggctg ggcggcccca cagacccgct
     6301 ggaacccagc agcggccgct tccctttagt gagggttaat gcttcgagca gacatgataa
     6361 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt
     6421 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta
     6481 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggagatgtgg gaggtttttt
     6541 aaagcaagta aaacctctac aaatgtggta aaatccgata aggatcgatc cgggctggcg
     6601 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
     6661 atggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     6721 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     6781 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     6841 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     6901 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     6961 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     7021 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     7081 tttaacgcga attttaacaa aatattaacg cttacaattt cctgatgcgg tattttctcc
     7141 ttacgcatct gtgcggtatt tcacaccgca tacgcggatc tgcgcagcac catggcctga
     7201 aataacctct gaaagaggaa cttggttagg taccttctga ggcggaaaga accagctgtg
     7261 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     7321 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     7381 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     7441 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     7501 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     7561 aggaggcttt tttggaggcc taggcttttg caaaaagctt gattcttctg acacaacagt
     7621 ctcgaactta aggctagagc caccatgatt gaacaagatg gattgcacgc aggttctccg
     7681 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
     7741 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
     7801 ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg
     7861 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
     7921 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
     7981 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
     8041 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
     8101 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
     8161 aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc
     8221 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
     8281 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
     8341 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
     8401 cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa
     8461 tgaccgacca agcgacgccc aacctgccat cacgatggcc gcaataaaat atctttattt
     8521 tcattacatc tgtgtgttgg ttttttgtgt gaatcgatag cgataaggat ccgcgtatgg
     8581 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca
     8641 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
     8701 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
     8761 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     8821 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     8881 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     8941 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     9001 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     9061 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     9121 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     9181 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     9241 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     9301 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     9361 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     9421 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     9481 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     9541 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     9601 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     9661 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     9721 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     9781 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     9841 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     9901 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     9961 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
    10021 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
    10081 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
    10141 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
    10201 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
    10261 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
    10321 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
    10381 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
    10441 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
    10501 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
    10561 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
    10621 tgctggcctt ttgctcacat ggctcgacag atct
//
