LOCUS       sequence-289070-        8709 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmAtx2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8709)
  TITLE     Izaurralde lab plasmids
REFERENCE   2  (bases 1 to 8709)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmAtx2_J
FEATURES             Location/Qualifiers
     source          1..8709
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2588..2653
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2678..2704
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5994..6035
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6045..6062
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6085..6102)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6135..6269
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6159..6178)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6213..6232
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(6891..7479)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6971..6990)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7650..8441,8442..8510))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8273..8292
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8511..8615)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8683..8701
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gacgcacaaa cacgacgacg tgagcgtcgc gctgagaaac aagctcaatg
     2641 gaaagctgca aacggtggat ctcctccaca tatggcttac ccatacgatg ttccagatta
     2701 cgctcctcca agcttgatga acaacaatag caagcggaaa acccgaccca ctggcggcgg
     2761 tgccagcggc ggcatctcaa gatacaactc caacgataac agcctaaggc ccactaacaa
     2821 caaggctggt gcaggaggcg ggaacggagg cgcagcagtg cgtccgtcgg cgcagggcgt
     2881 ctacaacaac acgtttttca tgcactcggc cacggcgctg gtgggcagcg ttgtggaggt
     2941 gcgcctgcgg tcgggcaata tctacgaggg cgtattccgc acattctcgg gcaactttga
     3001 catcgcactg gagctaccgg cgtgcattaa gtccaagaat ctgccggagg agggcaaggt
     3061 gccaaaacac attatattcc cggccgacac tgtggtgacc atcgtggcca aggactttga
     3121 ctcgcaatac gccaccgcag gcgcttttca gacggatggc gccatttctg acaagtgcaa
     3181 cggtgcgcgt ccggacgaga aggaattgga accatgggat tctggcgcta atggcgacat
     3241 tgacatagaa ttggatagtg ccgccaacgg ctgggatccc aacgagatgt tccgcaaaaa
     3301 tgagaacacg ttcggtgtga cctccacatt tgatgattct ctagcttcgt acacggtacc
     3361 gctagataag ggtgattccc tagaattcaa agaggccgaa gccaaggccg aaaagctggc
     3421 tgccgagatc gaaaataatc cgacgtgccg ggaccgtctg gacttggaaa atggtgatga
     3481 ggaggcgctc ttcgcggccg tggagcgtcc atcgaccgag caggatcaac gcggagatcg
     3541 tggcgaccgc gaacgcaacg atcgagatag ggagcgcgag gagcgggaca gggaccgtga
     3601 tcgtgatcgc ggtaacaagc cccgtggcgc tggtgacttc cagttgaggg agaccatgag
     3661 tagcgaccgc tacataacaa aacagacgcg cagtatcacc ggaccgcagc tatcgcatgt
     3721 tggcatgtcc tcgcagggca gcggacgcga tcgtgacact cgtggcgatg gcagcatgat
     3781 gatgcaaagt ggcggcgggt cgggccaagg cggctccacg cagtcgacgg cagcacttat
     3841 gctagccggc ggcctgaagg gcgtgggtcc ggccccctca gcaaacgcgt ccgcggactc
     3901 gtccagcaag tatagcggcg gttcgatggt aaagcgcaag acggtgcccc agggcggtaa
     3961 ggtgatgcgc aacaatgtgc ctacaggtgg cagcaacgtc tccgtgtcgc agggcggcaa
     4021 cggcaattct gtgggccaga acaagggcgg ctaccagccg tcgatgggca tgccgagtca
     4081 atactcctac cagggtaact cgcaaattat gcatgggtct tctcagtacc gcaaccaatc
     4141 tcacatgggc ggcgcaaata agctaaatgg cgattcgaac gccaatacca acaagccgct
     4201 gccacagcgc cagatgcgcc agtaccaggg ctctcagagc aactcgtcac tgaactatgg
     4261 cggtgaaccc cagtcgctgg gtaagcccgt gcacggctcc catggaggcc atcccgggca
     4321 aaatagcaac tcgccgccgc tgcagaccgc cggcccacag cagcaacagc agcaacagca
     4381 gcaacagcag cagcagcagc aacaacaaca gccgccgcag caacagcagc atcagaacat
     4441 acagccacaa gggcaaaaca cgcaaccagc gcgtcaggtg cgcacgcgcg ataaccaaat
     4501 gcaggaactg cgacagtttg gccaggactt ccaactggcc ccgagcaaca cttcaccgcc
     4561 ccagcagcaa cagcagcagc agcagcagca gcagcatcaa gtgcagcagc agcagcgagc
     4621 gttgcagcaa tctgcctcgc caccgcaaca gcagcagcag cagcagcaac aacagcagca
     4681 cgtggtcctg caccaagtgc cgcaaacgca cctgcatcaa gcggccctat cacagccaca
     4741 ctatgtgcct cagcagcagc ctcagcaggc gccgcttcca cagcagcaac atgtgccgca
     4801 tcacatgcaa caaaaggccc agcagcagca attggtggag acccagcatc agcatgtgca
     4861 gaagcagcac cagtcgcagc cacaagtgca gcagccgccg ccacagctgc tccaggatcc
     4921 atcacaacag ccgttgccaa tttaccatac aatgccgccg ccacagactt cacccgtagt
     4981 tgttacttcg ccggttctgc tggagcagcc tccgccacaa cccatgccgg tggtgcagca
     5041 acagcagacg cagcagcttg ccacgcccaa acctgaggtt tcgccggcac caccaagcag
     5101 caacaccacc acacctactg gcatagccag tacgcctaca gcaggggtga ttgccagcgc
     5161 tggatctgag aaaaccacgc cggcagcgcc cactccgacg agcaattccg caacggtgcc
     5221 tacaggaacg gccgccactg ctggaggtgc cacgggaacc actccggtgg ttaagaagca
     5281 tgtgctcaac ccatcagcca agccatttac accccgcggg cccagcaccc cgaatccatc
     5341 acgaccgcac accccacaga cgcctgtgcc catgacgaac atttacacaa ccacgggagg
     5401 acatgtaccg ccggcggcca accagccgat ctacgtcatg cagccacagc atccattccc
     5461 gcctcagaca cacccacagg ccggacaacc accgcgcctt cgccgtagta attaccctcc
     5521 aatggccgca tcgcagatgc acgtatcggc atccgcggcc acgggtcagc cgttgataac
     5581 agccggcccg ataccgcaat tcatccagta cggccatgca ccacaccagc aacaattcca
     5641 atcacatacg tacgcgccaa tgcagatgcg cgtgtacccg gaccagccgc agcagttgca
     5701 gtttatgaca cagacgccac agtcgaccac accgtcgccg ggccagccgc accagcagtt
     5761 ccatccgccg ccacagccat cccccgccgg cgggggacca caaccggcgt tcacgccgcc
     5821 cacacaggca gccacttacc agctgatgtg cgtgcacccc caatcgctct tagccaatca
     5881 ctacttcccg ccgccgacgc cgcagcatcc gcagcagaat caacaacagt atcagatcgt
     5941 gatgcagcag catcagccac agtgactcga gtctagaggg cccgcggttc gaaggtaagc
     6001 ctatccctaa ccctctcctc ggtctcgatt ctacgcgtac cggtcatcat caccatcacc
     6061 attgagttta aacccgctga tcagcctcga ctgtgccttc taaggcctga gctcgctgat
     6121 cagcctcgat cgaggatcca gacatgataa gatacattga tgagtttgga caaaccacaa
     6181 ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg
     6241 taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc
     6301 aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta
     6361 tggctgatta tgatcagtcg accgatgccc ttgagagcct tcaacccagt cagctccttc
     6421 cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa
     6481 ctcgtaggac aggtgccggc agcgctctgg gtcattttcg gcgaggaccg ctttcgctgg
     6541 agcgcgacga tgatcggcct gtcgcttgcg gtattcggaa tcttgcacgc cctcgctcaa
     6601 gccttcgtca ctggtcccgc caccaaacgt ttcggcgaga agcaggccat tatcgccggc
     6661 atggcggccg acgcgctggg ctacgtcttg ctggcgttcg cgacgcgagg ctggatggcc
     6721 ttccccatta tgattcttct cgcttccggc ggcatcggga tgcccgcgtt gcaggccatg
     6781 ctgtccaggc aggtagatga cgaccatcag ggacagcttc aaggatcgct cgcggctctt
     6841 accagccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     6901 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     6961 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     7021 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     7081 tctcaatgct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     7141 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     7201 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     7261 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     7321 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     7381 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     7441 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     7501 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     7561 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     7621 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     7681 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     7741 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     7801 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     7861 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     7921 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctgcagg catcgtggtg
     7981 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     8041 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     8101 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     8161 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     8221 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg ggataatacc
     8281 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     8341 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     8401 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     8461 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     8521 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     8581 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     8641 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
     8701 ccctttcgt
//
