LOCUS       sequence-289305-       11130 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of HsTNRC6C-F1389A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11130)
  AUTHORS   Huntzinger E, Braun JE, Heimstadt S, Zekri L, Izaurralde E
  TITLE     Two PABPC1-binding sites in GW182 proteins promote miRNA-mediated 
            gene silencing.
  JOURNAL   EMBO J. 2010 Dec 15;29(24):4146-60. doi: 10.1038/emboj.2010.274
  PUBMED    21063388
REFERENCE   2  (bases 1 to 11130)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/emboj.2010"; journalName: "EMBO J"; date: "2010-12-15- 15";
            volume: "29"; issue: "24"; pages: "4146-60"
COMMENT     Sequence Label: pAc5.1B-EGFP-HsTNRC6C-F1389A_L
FEATURES             Location/Qualifiers
     source          1..11130
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             join(2588..2590,2591..2593,2594..3304)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2588..2590,2591..2593,2594..3304)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2633..2654)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2894..2913)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3241..3262
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5593..5610
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             8415..8456
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             8466..8483
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(8506..8523)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8556..8690
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8580..8599)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8634..8653
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(9312..9900)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9392..9411)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10071..10862,10863..10931))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10694..10713
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10932..11036)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     11104..11122
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     2641 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
     2701 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     2761 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca
     2821 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     2881 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     2941 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     3001 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
     3061 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
     3121 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     3181 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca
     3241 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     3301 caagccaagc ttgatggcta cagggagtgc ccagggcaac ttcactggac ataccaagaa
     3361 gacaaatggc aataatggca ccaatggcgc actcgtccaa agcccttcta atcagagtgc
     3421 ccttggagca gggggagcga acagtaatgg aagtgcggcc agagtgtggg gtgtagccac
     3481 aggctccagc tctggcctgg ctcactgctc tgtcagtggt ggggatggaa aaatggacac
     3541 tatgattgga gatgggagaa gtcagaattg ctggggtgct tccaactcca atgctggcat
     3601 taatcttaac cttaatccta atgccaaccc agctgcctgg cctgtacttg gacatgaagg
     3661 aaccgtggcg acaggcaacc cttccagtat ttgcagtcca gtcagtgcca taggtcaaaa
     3721 tatgggcaac cagaacggga acccaacagg cactttaggt gcttggggaa acttgctgcc
     3781 acaagagagc acagaaccac aaacgtccac ttctcagaat gtgtctttca gcgcacaacc
     3841 tcagaacctt aacactgatg gaccaaataa cactaacccc atgaactctt cacccaaccc
     3901 tatcaatgca atgcagacaa atggactgcc aaactggggc atggctgttg gtatgggggc
     3961 catcatcccg ccccacctgc aaggccttcc tggtgctaat ggatcatcag tttctcaagt
     4021 cagtgggggc agtgctgaag gaataagcaa ttctgtgtgg ggactgtccc caggtaaccc
     4081 tgccacagga aatagcaatt ctgggttcag tcaggggaat ggagacactg tgaactcagc
     4141 attaagtgct aaacaaaatg gatccagcag tgctgtgcaa aaggaaggaa gtggaggaaa
     4201 tgcttgggat tcaggacctc ctgctggtcc tggaatactc gcctggggaa ggggcagtgg
     4261 caacaatggc gttggtaata tccattcagg agcttggggc caccccagcc gaagcacctc
     4321 taacggtgtg aatggggaat ggggaaagcc cccaaaccag cattccaaca gtgacatcaa
     4381 tgggaaagga tcaacagggt gggagagtcc tagtgtcacc agccagaacc ctaccgtaca
     4441 gcctggtggt gaacacatga actcctgggc caaagcggca tcttctggaa ctacagcaag
     4501 tgaaggaagt agtgatggtt ctggcaacca caatgaagga agcactggga gggaaggaac
     4561 gggagaaggc cgaaggcgag ataaagggat tatagaccaa gggcacatcc agttgccaag
     4621 gaatgatctt gacccaagag ttctgtctaa tactggttgg ggacagactc ctgtaaagca
     4681 aaacactgcc tgggaatttg aagaatcccc taggtctgaa aggaaaaatg acaatgggac
     4741 agaggcctgg ggttgtgcag ctactcaggc ttcaaactca ggggggaaga acgatgggtc
     4801 catcatgaac agtacaaata cctcttcagt atctgggtgg gtcaacgcgc cacctgccgc
     4861 tgtgccagca aacacaggtt ggggagacag caacaacaaa gcgccaagtg gcccgggggt
     4921 ttggggggac tcgataagct ctactgctgt tagtactgct gctgctgcca agagtggcca
     4981 tgcttggagt ggggccgcaa atcaggagga caagtcaccc acctggggtg agcctccaaa
     5041 gcccaaatcc caacactggg gagatggaca aagatcaaat ccagcctgga gtgcaggagg
     5101 gggagattgg gcagattcat cgtctgtcct tggacacttg ggggatggga aaaaaaatgg
     5161 atctggatgg gatgctgaca gtaataggtc agggtctggt tggaatgaca ccacgagatc
     5221 tgggaacagt ggctggggca acagcacaaa tacaaaggcc aatccaggta caaactgggg
     5281 ggagacttta aaacctggcc cccaacagaa ctgggctagc aaaccccaag acaacaatgt
     5341 gagtaactgg ggaggagctg cttctgtgaa acagacagga acagggtgga tcggggggcc
     5401 ggtaccggtc aaacagaagg acagcagtga agcaactggc tgggaagaac cctctccacc
     5461 gtccattcgc cgcaaaatgg aaattgatga tggtacctca gcttgggggg acccaagcaa
     5521 ctataacaat aaaactgtaa acatgtggga tagaaacaac ccggtcatcc agagcagtac
     5581 cacgaccaat accaccacca ccaccaccac taccacgagc aacaccacac acagggtcga
     5641 gacgccgccc ccgcaccagg ctggtactca gctgaatcga tcaccgttgc ttggtccagg
     5701 taggaaagtt tcatcaggct ggggagaaat gcctaatgtt cactcaaaga ctgaaaactc
     5761 ttggggagaa ccatcctccc cttctaccct ggtggataat ggcacagcag catgggggaa
     5821 gccacccagc agtggcagcg ggtggggaga tcaccctgcc gagccgccgg tggcatttgg
     5881 aagagctggc gcacctgttg ctgcctcagc cctgtgcaaa ccagcttcaa aatctatgca
     5941 agaaggctgg ggcagtggtg gggatgaaat gaacctcagt accagccagt gggaggatga
     6001 agaaggggac gtgtggaata atgctgcttc ccaagaaagc acctcctcct gcagctcctg
     6061 ggggaacgcc cccaaaaaag gacttcaaaa gggcatgaag acgtctggca agcaggatga
     6121 ggcctggatc atgagccggc tgatcaaaca actcacagac atgggcttcc cgagagagcc
     6181 agctgaggag gccttgaaga gtaacaatat gaatcttgat caggccatga gcgctctgct
     6241 ggaaaagaag gtggacgtgg acaagcgtgg gctgggagtg accgaccata atggaatggc
     6301 cgccaagccc ctcggctgcc gcccgccaat ctccaaagag tcttccgtgg accgccccac
     6361 ctttcttgac aaggatggcg gcctcgtgga agagcccacg ccttcaccgt tcttgccttc
     6421 cccaagcctg aagctccccc tttcacacag tgcactcccc agtcaggccc tgggtgggat
     6481 tgcctccggg ctgggcatgc aaaacttgaa ttcttctaga cagataccga gtggcaatct
     6541 gggtatgttt ggcaatagtg gagcagcaca agccaggacc atgcagcagc cgccacagcc
     6601 accagtgcag cctcttaact cttcccagcc cagtctccgt gctcaagtgc ctcagtttct
     6661 atcccctcag gttcaagcac agcttttgca gtttgcagca aaaaacattg gtctcaaccc
     6721 tgcactatta acctcgccaa ttaatcctca acatatgacg atgttgaacc agctctatca
     6781 gctgcagctg gcataccaac gtttacaaat ccagcagcag atgttacagg cccagcgtaa
     6841 tgtgtccgga tccatgagac aacaggagca gcaagttgcg cgcacaatca ctaatctgca
     6901 gcagcagatc cagcagcacc agcgccagct ggcccaggcc ctgctcgtga agcagccacc
     6961 accgccaccg cccccgccgc acctgtctct gcacccctct gcaggcaaat cggccatgga
     7021 cagcttcccc tcgcacccac agactcccgg cctacctgac ctgcagacca aagagcagca
     7081 gtcttcaccc aacacctttg ctccttaccc tctcgctgga ctgaacccaa acatgaatgt
     7141 caacagcatg gacatgaccg gtggcttgtc ggtgaaggac ccatcccagt cccagtcacg
     7201 cctcccccag tggacgcacc ccaactccat ggataacttg cccagtgccg cttcccccct
     7261 ggagcagaac cctagcaagc atggtgctat ccctggaggt ctaagcattg ggcctccagg
     7321 taagtcctcc attgatgact cctatggccg gtacgattta atccagaaca gtgagtcacc
     7381 agccagtcct cccgtagctg ttccccatag ctggtcacgt gccaaatctg acagtgataa
     7441 aatctcaaat ggctctagca tcaactggcc cccagaagcc catccgggag ttccatggaa
     7501 aggactgcag aatattgacc ctgagaatga ccctgacgtc actcctggca gtgtccccac
     7561 tgggcctacc atcaacacca ccatccagga tgtcaaccgc tacctcctca agagtggagg
     7621 taaactgtca gacatcaaat cgacgtggtc ctctggccct acctcccaca cgcaagcctc
     7681 tctgtctcat gaactatgga aggtgcccag aaacagtact gcacccacga ggccacctcc
     7741 agggttaacc aatcccaagc cctcctccac ctggggtgcc agccccctcg gctggaccag
     7801 ctcctactcc tcgggttctg cctggagcac cgacacctca ggaagaacca gcagctggct
     7861 cgttcttcga aacctcactc cccagattga tggttctaca ctgcggacat tgtgtttgca
     7921 acatgggcct cttatcacat tccacctgaa tctgactcaa ggcaatgctg tggtccggta
     7981 cagctccaag gaggaggctg ccaaggccca gaagtctctg cacatgtgcg tcctgggaaa
     8041 cactaccatc ctggccgagt tcgctggtga agaagaagtg aatcgcttct tagcccaagg
     8101 ccaggcgctg ccacccactt ccagctggca gtccagcagc gcgtccagcc agccgcggct
     8161 cagcgcagcg ggcagctccc atggcctggt acgcagcgac gctggccact ggaacgcccc
     8221 gtgcctgggt ggcaagggga gcagtgagct gctgtggggc ggggtgcccc agtactccag
     8281 cagcctgtgg ggcccgccca gcgccgacga cagcagggtg ataggcagcc ccacgccgct
     8341 aaccaccctg ctgcctgggg acctgctcag cggggagtcc ctgtagctcg agtctagagg
     8401 gcccgcggtt cgaaggtaag cctatcccta accctctcct cggtctcgat tctacgcgta
     8461 ccggtcatca tcaccatcac cattgagttt aaacccgctg atcagcctcg actgtgcctt
     8521 ctaaggcctg agctcgctga tcagcctcga tcgaggatcc agacatgata agatacattg
     8581 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt
     8641 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca
     8701 attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt
     8761 aaaacctcta caaatgtggt atggctgatt atgatcagtc gaccgatgcc cttgagagcc
     8821 ttcaacccag tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg
     8881 actgtcttct ttatcatgca actcgtagga caggtgccgg cagcgctctg ggtcattttc
     8941 ggcgaggacc gctttcgctg gagcgcgacg atgatcggcc tgtcgcttgc ggtattcgga
     9001 atcttgcacg ccctcgctca agccttcgtc actggtcccg ccaccaaacg tttcggcgag
     9061 aagcaggcca ttatcgccgg catggcggcc gacgcgctgg gctacgtctt gctggcgttc
     9121 gcgacgcgag gctggatggc cttccccatt atgattcttc tcgcttccgg cggcatcggg
     9181 atgcccgcgt tgcaggccat gctgtccagg caggtagatg acgaccatca gggacagctt
     9241 caaggatcgc tcgcggctct taccagccag caaaaggcca ggaaccgtaa aaaggccgcg
     9301 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     9361 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     9421 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     9481 ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag
     9541 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     9601 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     9661 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     9721 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
     9781 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     9841 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     9901 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     9961 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
    10021 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
    10081 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
    10141 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
    10201 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
    10261 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
    10321 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
    10381 attgctgcag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
    10441 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
    10501 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
    10561 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
    10621 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
    10681 gcgtcaacac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
    10741 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
    10801 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
    10861 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
    10921 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
    10981 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
    11041 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
    11101 aaaaataggc gtatcacgag gccctttcgt
//
