LOCUS       sequence-28953-e         731 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Zhu G, Wu CJ, Zhao Y, Ashwell JD
  TITLE     Optineurin negatively regulates TNFalpha- induced NF-kappaB 
            activation by competing with NEMO for ubiquitinated RIP.
  JOURNAL   Curr Biol. 2007 Aug 21. 17(16):1438-43.
  PUBMED    17702576
REFERENCE   2  (bases 1 to 731)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr Biol. 
            2007 Aug 21. 17(16):1438-43."
COMMENT     Sequence Label: pDEST17-hOPTN-D474N
FEATURES             Location/Qualifiers
     source          1..731
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             57..74
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    84..108
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             443..454
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 gnnttctctg aatattttgt ttactttaag aaggagatat acatatgtcg tactaccatc
       61 accatcacca tcacctcgaa tcaacaagtt tgtacaaaaa agcaggctcc gcggccgccc
      121 ccttcacctc ccatcaacct ctcagctgcc tcactgaaaa ggaggacagc cccagtgaaa
      181 gcacaggaaa tggacccccc cacctggccc acccaaacct ggacacgttt accccggagg
      241 agctgctgca gcagatgaaa gagctcctga ccgagaacca ccagctgaaa gaagccatga
      301 agctaaataa tcaagccatg aaagggagat ttgaggagct ttcggcctgg acagagaaac
      361 agaaggaaga acgccagttt tttgagatac agagcaaaga agcaaaagag cgtctaatgg
      421 ccttgagtca tgagaatgag aaattgaagg aagagcttgg aaaactaaaa gggaaatcag
      481 aaaggtcatc tgaggacccc actgatgact ccaggcttcc cagggccgaa gcggagcagg
      541 aaaaggacca gctcaggacc caggtggtga ggctacaagc agagaaggca gacctgttgg
      601 gcatcgtgtc tgaactgcag ctcaagctga actccagcgg ctcctcagaa gattcctttg
      661 ttgaattagg atggctgaag gagaagcaga agggtcagta aaagaaatca agcatagtcc
      721 tggccaaaaa a
//
