LOCUS       sequence-289601-       10234 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmGW182-Nterm6Wmut-SD6mut.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10234)
  AUTHORS   Huntzinger E, Kuzuoglu-Ozturk D, Braun JE, Eulalio A, Wohlbold L, 
            Izaurralde E
  TITLE     The interactions of GW182 proteins with PABP and deadenylases are 
            required for both translational repression and degradation of miRNA 
            targets.
  JOURNAL   Nucleic Acids Res. 2013 Jan;41(2):978-94. doi: 10.1093/nar/gks1078. 
            Epub 2012 Nov 21.
  PUBMED    23172285
REFERENCE   2  (bases 1 to 10234)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gks1078"; journalName: "Nucleic Acids Res"; date: 
            "2013-01"; volume: "41"; issue: "2"; pages: "978-94"
COMMENT     Sequence Label: pAc5.1B-EGFP-DmGW182-Nterm6Wmut-SD6Wmut_O
FEATURES             Location/Qualifiers
     source          1..10234
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             join(2588..2590,2591..2593,2594..3304)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2588..2590,2591..2593,2594..3304)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2633..2654)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2894..2913)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3241..3262
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             7519..7560
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             7570..7587
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7610..7627)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7660..7794
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7684..7703)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7738..7757
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(8416..9004)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8496..8515)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9175..9966,9967..10035))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9798..9817
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10036..10140)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10208..10226
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     2641 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
     2701 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     2761 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca
     2821 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     2881 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     2941 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     3001 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
     3061 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
     3121 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     3181 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca
     3241 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     3301 caagccaagc ttgaattctc ctgcaggcat gcgtgaagcc cttttttccc aagatggctg
     3361 gggctgtcag catgttaacc aggatactaa ttgggaagtt cccagttcgc cagaaccagc
     3421 caataaggat gcacccggtc caccaatgtg gaagccaagc attaacaatg gtactgatct
     3481 ttgggagtcc aatttgagaa acggaggtca gccggccgca cagcaagttc caaagccgtc
     3541 gtggggtcat acaccatcct ctaacttagg tggaacatgg ggtgaggacg acgatggcgc
     3601 cgatagtagt agtgtgtgga ctggaggagc tgttagcaac gcgggatccg gagctgcagt
     3661 gggagtaaac caagccggag ttaatgtcgg tccaggcggt gttgtttcgt ctggcggacc
     3721 tcagtgggga caaggtgtcg ttggcgtcgg acttggatca actggaggta acgggtcaag
     3781 caatataact ggatcgtctg gagtcgcaac aggtagtagc ggaaactcca gcaacgctgg
     3841 taacggttgg ggagaccctc gtgaaatacg ccctttggga gttggtggct ccatggatat
     3901 tcgaaatgtt gaacatcgcg gcggtaacgg ttctggagca acttcgagcg atccacgaga
     3961 cattcgcatg atcgatccgc gtgaccctat tcgaggagat ccccgtggaa tatctggtcg
     4021 tcttaatggg acctctgaaa tggcgggtca tcatccacaa atgtcccata accagttgca
     4081 aggtatcaac aaaatggttg gtcaaagtgt agcaactgcc agcaccagtg tcggaacatc
     4141 tggctcgggc atcggtcctg gaggtcccgg tcctagtaca gtatcaggca atatcccaac
     4201 acaggcgggg cctgctcaac cggtaagcgt tggtgtaagt ggtcccaaag acatgtcaaa
     4261 acagataagt ggagcggagg aaccatcacc accgcctcag cgtcgcagta ttcctaacta
     4321 cgatgatggt acatcgttgg cgggtcagca aactcgtgtt cccgctgcaa gcggtcactg
     4381 gaaagacatg actgattcga taggtcgtag tagtcatctc atgcgtggcc aaagccaaac
     4441 gggaggtata ggaatagccg gcgttggaaa tagcaatgtt ccagtgggag ccaatccaag
     4501 taatcctata agcagtgtag ttggacctca agcccggatt ccatctgtgg gcggcgtaca
     4561 acacaaacca gacggcggcg ctatgtgggt gcattccggc aatgtaggtg gcagaaataa
     4621 tgttgctgct gttactactg cgggagatga cactcatagc gttaatgtcg gcgctcccag
     4681 cagtggcagt gtatccagca acaattgggt tgatgacaag tccaactcaa ccttggcaca
     4741 aaactcttgg agcgacccgg cccctgttgg agttagtgcg ggcaataagc aaagcaaacc
     4801 gccaagcaat agtgcttcat caggttggag cactgctgcg ggcgtggtgg atggggttga
     4861 tctaggatct gagtggaaca cgcacggggg gattattgga aaatctcagc aacaacaaaa
     4921 actagcggga cttaacgtgg gaatggtgaa cgtaattaac gcggagatca ttaagcaaag
     4981 caagcaatac aggatccttg tcgagaacgg ctttaaaaag gaagatgtag agcgggcatt
     5041 agtgattgct aatatgaaca tcgaagaggc agccgatatg ctccgtgcca actcatccct
     5101 atcaatggat ggttggcgtc gacatgatga gtcccttgga tcttatgccg accacaatag
     5161 ttcaacaagc agcggtggat ttgctggtcg ttacccggtc aacagtggac aaccttcaat
     5221 gtcctttcct cataataacc ttatgaataa catgggaggt accgctgtta ctggaggtaa
     5281 caacaataca aacatgacag ctttacaggt gcaaaagtat ttaaatcaag ggcaacatgg
     5341 tgtcgctgtt ggaccgcaag ccgttggtaa ttcttcagca gtatctgtcg gatttggtca
     5401 gaacacgtct aacgcagcag tggcaggagc agcctctgca aatatagcag caaatacaaa
     5461 caaccaaccg tctggtcagc aaattcgcat gctaggccag caaattcagt tggccattca
     5521 tagtggtttc atatctagtc agatattgac tcaaccgcta actcaaacaa cccttaacct
     5581 tttaaaccaa cttcttagca atattaagca tctccaggct gcacagcaat cccttacccg
     5641 cgggggaaat gtcaatccaa tggcagtgaa tgtggctata tctaaataca agcagcaaat
     5701 ccagaattta cagaaccaga taagtgcaca acaggctgtg tatgtaaaac agcaaaatat
     5761 gcaaccaact tcacaacaac aacagcccca acaacagcaa cttccttctg ttcatctaag
     5821 taactcaggc aacgactatt taagaggtca cgatgcaata aataatttgc aaagcaactt
     5881 ttctgagctc aatattaata agccaagtgg atatcaagga gcgtccaatc aacaatcccg
     5941 attaaatcag tggaagcttc cagtattaga taaggagatc aactctgaca gtacggaatt
     6001 ttctcgtgcc ccaggtgcaa cgaaacaaaa tttgacggcc aacacaagca acataaactc
     6061 tttgggtctt caaaacgata gtacatggtc aactggacgc agtattggtg acggttggcc
     6121 tgatccctca tctgataacg agaataaaga ctggtctgtt gctcagccaa cttcagcagc
     6181 aactgcttac actgatctgg tccaagagtt tgagccaggc aagccatgga agggttcaca
     6241 gatcaaaagc atagaagatg atcccagcat tacaccagga agcgttgcta gatctccatt
     6301 gtctattaat tcgacgccaa aagatgctga catatttgcc aataccggta aaaattcacc
     6361 gactgattta ccgccactaa gtttatcgtc gtctacagca agttttaatc caaaccaaaa
     6421 ttatccgagt cacagtgcat ctgacaatag tcaacaatgt accgccactt cggagcttgc
     6481 aacaagcccg ctaaataaat catcgtctcg aggtcccccg ccaggattga ctgccaattc
     6541 aaataaatct gcaaatagta atgcgtcaac gccaacaact attaccggag gtgcgaatgg
     6601 agcattacag cctcgaagtg gcggtgttca aaccacaaac actaatgcga caggtggtaa
     6661 caccactgcg ggctccagtt ggttgctttt gaaaaatcta acagcacaga ttgatggtcc
     6721 tactttgcgt acactgtgta tgcagcatgg gccccttgtc agctttcacc cgtatttgaa
     6781 ccaaggaatt gccttatgta aatatactac tcgtgaggag gcgaacaagg cgcaaatggc
     6841 gttaaacaac tgtgtcctcg ccaacaccac aatatttgct gaatctccca gcgagaacga
     6901 ggtgcaaagc attatgcagc acttaccaca aactccttcc tctacaagct ctagtggaac
     6961 tagtggtggc aacgtcggag gcgtcggcac ttcagccaat aatgcaaaca gtggttctgc
     7021 agcttgtctg tccggaaaca atagcggcaa cggaaacggc agcgcgagcg gcgccggcag
     7081 cggcaacaat ggcaacagta gctgcaacaa cagtgccgcc ggggggggca gcagcagcaa
     7141 caacacgatt accactgtag caaattcgaa tcttgttggt tctagtggct ctgtctcaaa
     7201 ttcctctggc gttactgcta actctagtac tgtttctgta gttagttgta cagcgagtgg
     7261 gaattccata aatggggcag gtactgcaaa cagttctggt tcaaagagta gtgcaaacaa
     7321 tttagctagc ggccagtcta gcgcttctaa cttaactaat agcaccaatt caacatggcg
     7381 acaaactagc caaaaccaag ctcttcaaag tcaaagcagg ccatcaggca gagaagctga
     7441 ctttgattat atatctctcg tttattccat tgttgatgat taagcggccg ctcgagtcta
     7501 gagggcccgc ggttcgaagg taagcctatc cctaaccctc tcctcggtct cgattctacg
     7561 cgtaccggtc atcatcacca tcaccattga gtttaaaccc gctgatcagc ctcgactgtg
     7621 ccttctaagg cctgagctcg ctgatcagcc tcgatcgagg atccagacat gataagatac
     7681 attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa
     7741 atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac
     7801 aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc
     7861 aagtaaaacc tctacaaatg tggtatggct gattatgatc agtcgaccga tgcccttgag
     7921 agccttcaac ccagtcagct ccttccggtg ggcgcggggc atgactatcg tcgccgcact
     7981 tatgactgtc ttctttatca tgcaactcgt aggacaggtg ccggcagcgc tctgggtcat
     8041 tttcggcgag gaccgctttc gctggagcgc gacgatgatc ggcctgtcgc ttgcggtatt
     8101 cggaatcttg cacgccctcg ctcaagcctt cgtcactggt cccgccacca aacgtttcgg
     8161 cgagaagcag gccattatcg ccggcatggc ggccgacgcg ctgggctacg tcttgctggc
     8221 gttcgcgacg cgaggctgga tggccttccc cattatgatt cttctcgctt ccggcggcat
     8281 cgggatgccc gcgttgcagg ccatgctgtc caggcaggta gatgacgacc atcagggaca
     8341 gcttcaagga tcgctcgcgg ctcttaccag ccagcaaaag gccaggaacc gtaaaaaggc
     8401 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     8461 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     8521 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     8581 tctcccttcg ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc tcagttcggt
     8641 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     8701 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     8761 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     8821 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
     8881 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     8941 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     9001 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     9061 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     9121 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     9181 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     9241 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     9301 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     9361 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     9421 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     9481 tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     9541 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     9601 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     9661 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     9721 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     9781 cccggcgtca acacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     9841 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     9901 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     9961 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
    10021 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
    10081 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
    10141 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
    10201 ctataaaaat aggcgtatca cgaggccctt tcgt
//
