LOCUS       sequence-290031-        9675 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmCup_1-417-DmeIF4G_686-1666.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9675)
  AUTHORS   Igreja C, Peter D, Weiler C, Izaurralde E
  TITLE     4E-BPs require non-canonical 4E-binding motifs and a lateral surface
            of eIF4E to repress translation.
  JOURNAL   Nat Commun. 2014 Sep 2;5:4790. doi: 10.1038/ncomms5790.
  PUBMED    25179781
REFERENCE   2  (bases 1 to 9675)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-09-2"; volume: "5:4790. doi"; pages: " 
            10.1038/ncomms5790"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmCup_1-417-DmeIF4G_686-1666_T
FEATURES             Location/Qualifiers
     source          1..9675
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2588..2653
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2678..2704
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6960..7001
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             7011..7028
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7051..7068)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7101..7235
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7125..7144)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7179..7198
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(7857..8445)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7937..7956)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8616..9407,9408..9476))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9239..9258
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9477..9581)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9649..9667
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gacgcacaaa cacgacgacg tgagcgtcgc gctgagaaac aagctcaatg
     2641 gaaagctgca aacggtggat ctcctccaca tatggcttac ccatacgatg ttccagatta
     2701 cgctcctcca agcttgaatt ctgcagatat catggaccct atgaaagagg aggcgatcga
     2761 attgaatggc catgctatgc aaatggccga agctgagcaa gaaaatggcg ctggcgccct
     2821 gaaaattgcc accaatgcgg gtgcaaccga tcgtccagcg catcagcagc ttccgctgcc
     2881 agtcgaagat cagcaggatg aggtgctgac ccccgctgag aagggcaagt tcgagtaccc
     2941 gccccctcca ccgccaccaa ctccagtcca ggctccgcta gccaccaagg ccaccgctct
     3001 gaatgccagt caagagcatg acgacgacga ggccaactcc gagaagtggg aggatccgtg
     3061 cgctccgccc ccgcctcctc cgctgccgac tagcgctttc ctggccaccg gactgggcta
     3121 cctcaagctg cccgccttca agctgaagga cgcactggag aaggccatca ccaagttgga
     3181 ggccaacaag cggacgctaa aggccagccc tgagtcttct cgttccatca aaaacaagaa
     3241 tgtggtggcc ctggagatgt tacccaggcg ctcaaaccca gaaacgatcg gcgatggatc
     3301 catgttggca tccacatcca cggctgttat gctgcagacc aaaaagcccg ccgttattgt
     3361 ggagatggag aggcgttgta agatcatcaa tttactggcc aaacagaatc aaatcctcga
     3421 atccatttcg ggggaggcca ttccgatgca tggaccctcg aagcatctgc acgaggacga
     3481 gggtctgacc ctgcaagttc tctcggcccg agcatccaca ccgtacacac agccctccag
     3541 catgttgtcc tgcacggccg ttagttgtga cctggagcac gattcgccgc ggaagcaagt
     3601 ggccagcaag gaggccgtgc cggagcagca gtctagtcag gtgcagcaaa agcgtccacc
     3661 cagcacaggc attcacaagc caggaagtct gcgagcaccc aaggctgttc gtcctactac
     3721 tgctccggtg gtcagcagca agccggtcaa gagctatacc cgctcccgcc tgatggacat
     3781 tcgcaacggg atgttcaatg ccctgatgca caggtccaag gagagctttg tgatgcctcg
     3841 catcgccacc tgcgacgaca ttgagttgga gggaaggctt cgccgcatga atatttggcg
     3901 cacctcggac ggcacaagat tccggacccg ctccaccaca gctaacttaa acatgaacaa
     3961 caacaacaac aatgagtgca tgttaattaa tatgaaacag atgtcgatgt ctggcgtaca
     4021 aagtggcggc ggtagaagca gtatgaaggg catgatacac gtaaatttat cattaaatca
     4081 agatgtaaag ttaagtgaaa atgaaaatgc atggcgccct cgggtcttaa ataaatctga
     4141 tggtgatagc gacgcgaaat ctgcactcga aaaagatgaa ttggtacgtc gtgttcgggg
     4201 tattttaaat aagcttaccc cagaaaggtt tgacacactt gttgaggaga ttataaaatt
     4261 gaaaattgat acacccgaca aagtggacga agtaattgtg ttggtatttg aaaaggccat
     4321 tgatgagcca aacttttctg tgtcttacgc cagactatgt caacgacttg ctgcggaagt
     4381 gaaggtaata gacgagcgca tggaaagcga aaccaaatct aactcggcac actttcgaaa
     4441 tgcattgtta gataaaactg aacaggaatt cacccaaaac gtttcacaaa gcaccgcaaa
     4501 ggaaaaaaaa ttacagccaa ttgtggataa aattaagaaa tgtactgatg ccaatgaaaa
     4561 agcggaactt gaggcatttt tggaagaaga agaaagaaaa ataagaagac gatccggagg
     4621 gacagtacga tttattggcg aactttttaa gatatctatg ttgactggca aaataatata
     4681 ctcttgcatc gatacacttc ttaaccctca ttctgaagac atgcttgagt gtttgtgcaa
     4741 gctgcttaca actgtgggtg ctaaatttga aaagacccca gtaaattcca aagaccctag
     4801 tcggtgttat tcgttggaaa aatcgatcac caggatgcag gccatcgcat cgaaaactga
     4861 caaagatgga gccagagtaa gctcaagagt acgatttatg cttcaagacg ttatcgactt
     4921 gcgtaagaat aaatggcaaa cttcgcggaa tgaagcaccc aaaactatgg gacaaattga
     4981 aaaagaggca aagaatgagc agctttctgc acaatatttt ggtacacttt ccagtactac
     5041 acccggagga tcgcagggcg gctctggtaa acgagatgac cgtggaaact ctcgatacgg
     5101 tgaatctcgt tcaagtagcg cgtatggtgg cagccacagt caacgtggtg ataatggcaa
     5161 ccttcgacac cagcagcaga acaacgtcgg gggaaacgtc tctggaggtg ctggacactc
     5221 caatggaaac aatgatgaaa atacttggca tgtgcaaacc agcaaaggta gccgctctct
     5281 agcggttgac agcaataaac tggaaggcct gtctaaactt tctgaccaaa atctggaaac
     5341 aaagaagatg ggtggattaa cgcaattcat ttggattagt agtgacacga caagactatc
     5401 gtcggcgccg acaccaaccc catccaatcc tttcgctgtg ctctcatccc taattgataa
     5461 aaatagtaat gaaagggatc gcgatcgcag tggacccaga aacaaaggat catacaacaa
     5521 aggttctatg gagcgggatc gttatgatag aggtatgcat tcgagaactg gatcatctca
     5581 gggatcacga gaaaactcat cctcccgcgg tggtcagcag ggtcgaactt tattgagttc
     5641 atcagttcaa aaatctacta gtcattcaaa atacacccaa caggcaccac ctacacggca
     5701 tactgttaaa gcccagtcat cagtgggcag ctctaacgtt aacacaggtc cactctaccg
     5761 tggaagtgag cagcaaacat cagcaacctt ctcgcagacc acccgttctg ttgctcctgt
     5821 agcagtgttc atcgaagcta gcgaaacaga tttaaaactc atcaaatctg ttgtttcaga
     5881 aattgtagac ctatcagctg catctaaaga agtcacgccc ggtgcagttt cgtgcataaa
     5941 acgagtacca gagaaactac ggtgcagttt catttattat attttaacag attatttgca
     6001 tttagcaaat gtaggtaaac agtacaggcg gtatttatct attgccgtct cccaacttat
     6061 tcagcagaat tacatttctg ccgatcattt gagactggct tacaacgaat ttaccgtata
     6121 tgcaaacgat ttgattgttg atattccgga gctttggcta tacattcttc aatttgccgg
     6181 gccactaatt gtaaagaaaa tattgactat atcagatttg tggaacaaca atttaaaaga
     6241 aaatagtcca tcaaacgtcg ctaaaaagtt ccttaaaaca tacttgatat attgcacaca
     6301 agaagttgga ccaaattttg ctcggaatat gtggatcaag tttaacctaa aatggtcaga
     6361 ttttatgcct gaaagtgaag ttgccgactt tattaaattt aacagactgg aatatgtgga
     6421 aaacgaatca aagtcgccag tgattgatca tagagagact ccagaaaagc acgttaaaaa
     6481 tgtgatagac catattgagc acttactaaa ggaaggaaca acggcggact gcattattga
     6541 ttatagtaac ggaaatataa tggtggttga taagttgttt atcagaggat tgacggaaac
     6601 attgagtaat ttctcgattc actacaagga caacagttac aagcttgagt ccgaaacctt
     6661 tcaaaagttt tgcataccgg ttttgcagag atacattgat tctaatgaag accaccaact
     6721 tgaatgttta tatactttgc agcttttagt tcatggattg gagcatccaa gagggttgct
     6781 gagtgaacta attggcgagc tatatgatgc ctttgtaata cagaaggaat cgctttgtaa
     6841 gtggcgagat tcaaaggatc agtctgctgg caagggcgtt gctgtaaaaa gtcttaatcc
     6901 tttttttaac tcgttgttaa acgatgatgc caacagttct agagggcccg cggttcgaag
     6961 gtaagcctat ccctaaccct ctcctcggtc tcgattctac gcgtaccggt catcatcacc
     7021 atcaccattg agtttaaacc cgctgatcag cctcgactgt gccttctaag gcctgagctc
     7081 gctgatcagc ctcgatcgag gatccagaca tgataagata cattgatgag tttggacaaa
     7141 ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt
     7201 tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta
     7261 tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat
     7321 gtggtatggc tgattatgat cagtcgaccg atgcccttga gagccttcaa cccagtcagc
     7381 tccttccggt gggcgcgggg catgactatc gtcgccgcac ttatgactgt cttctttatc
     7441 atgcaactcg taggacaggt gccggcagcg ctctgggtca ttttcggcga ggaccgcttt
     7501 cgctggagcg cgacgatgat cggcctgtcg cttgcggtat tcggaatctt gcacgccctc
     7561 gctcaagcct tcgtcactgg tcccgccacc aaacgtttcg gcgagaagca ggccattatc
     7621 gccggcatgg cggccgacgc gctgggctac gtcttgctgg cgttcgcgac gcgaggctgg
     7681 atggccttcc ccattatgat tcttctcgct tccggcggca tcgggatgcc cgcgttgcag
     7741 gccatgctgt ccaggcaggt agatgacgac catcagggac agcttcaagg atcgctcgcg
     7801 gctcttacca gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     7861 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     7921 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     7981 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     8041 gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     8101 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     8161 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     8221 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     8281 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     8341 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     8401 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     8461 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     8521 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     8581 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     8641 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     8701 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     8761 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     8821 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     8881 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tgcaggcatc
     8941 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     9001 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     9061 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     9121 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     9181 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aacacgggat
     9241 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     9301 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     9361 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     9421 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     9481 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     9541 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     9601 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     9661 acgaggccct ttcgt
//
