LOCUS       sequence-290120-        7840 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmPAN3-L676EG683E-del460-465.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7840)
  AUTHORS   Christie M, Boland A, Huntzinger E, Weichenrieder O, Izaurralde E
  TITLE     Structure of the PAN3 pseudokinase reveals the basis for 
            interactions with the PAN2 deadenylase and the GW182 proteins.
  JOURNAL   Mol Cell. 2013 Aug 8;51(3):360-73. doi: 
            10.1016/j.molcel.2013.07.011.
  PUBMED    23932717
REFERENCE   2  (bases 1 to 7840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2013.07"; journalName: "Mol Cell"; date: 
            "2013-08-8- 8"; volume: "51"; issue: "3"; pages: "360-73"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmPAN3-L676EG683E-del460-465_U
FEATURES             Location/Qualifiers
     source          1..7840
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2588..2653
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2678..2704
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5125..5166
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5176..5193
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5216..5233)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5266..5400
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5290..5309)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5344..5363
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(6022..6610)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6102..6121)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6781..7572,7573..7641))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7404..7423
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7642..7746)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7814..7832
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gacgcacaaa cacgacgacg tgagcgtcgc gctgagaaac aagctcaatg
     2641 gaaagctgca aacggtggat ctcctccaca tatggcttac ccatacgatg ttccagatta
     2701 cgctcctcca agcttgaatt ctatgtgctc gacttatgga ctcttatctt ttgagttttc
     2761 agatacggct gctatggatc caattttctt ctcgccaacg aatggaatac cgtcagagag
     2821 taaactcgcc acttatatga atcgacagaa tgtcgccaca ccgagcggtt atgggctgaa
     2881 taacgggttc tcactactta atttggattc gcccctaaat aaaaagtccc aggtcacgcc
     2941 gcagtcgccc gagttcattc caacgcgcct tggctcgacg cccaacttct atgcaccata
     3001 tcacaacgcg cccatgcaat taagtaatgg gctcaatggc gtcaatgggt taacagccgc
     3061 cgcagcagca gcagcagcag ccgccgccgc cgccgcagca gcagttccct cctccacgtc
     3121 atcggcgtcc tccgcctcgg tgaccatcag caaaacggcc aacggtggcg cctccatgct
     3181 cgccctgcaa aagaccgcat ccatcgcctc tgtaaccatc gcacaacagc agccacagca
     3241 tccacagaaa cagcaacagc atccgccgtc ggtgggcgga ggagctgtgt ccgccgcctc
     3301 cgcccccatt gccattagtg gcgctccacc gaatcccgcc gcagctccgt tcgtcagcag
     3361 catgtccgct cagacgccgc tcaaggggag gggcgccatg atgcgccagg aatcaccaac
     3421 ggcagccatg atttcgggcg cagggccagg tgagaagtcg ccaccgcacg ggatgacgcc
     3481 gcacggagca tcgcccatac catcagcgct gcccaccagc gtgcaccagg agaacgtagg
     3541 cggcaccata tacttctatc cgactgccaa cgcacagaac agccagcccg tcgtcaattc
     3601 catggtggtg gatggaacgc atccggctct ccatggcgtg agtgctgtgg cgccgatgag
     3661 tgcgggcgtt ccggcggcca tgatgtacac ggggcatgtg tatccgggac cgtcctccaa
     3721 cgtggtcacc atgcagccaa agacgctgct ggagtcggcc ttctttatgc ccgacgagat
     3781 gcgcgccgaa gtcctcgccc gcaacgagat ctctaacctg atcatggacg cagcggaggc
     3841 tgcgcagcac gctctaccgc tggaggtgga gaactaccat gccctgtatc cactggaacc
     3901 gccggcgcag ccgttgcacg ccaagctcac gttccccgcc accacttata gggccacgca
     3961 caacacgacg ggctacaagt actgcctgcg aagaatacat ggtttccgcc tgcagtcgac
     4021 caagtgcatg acgctggtgg agatgtggaa gaagctgcag cacacgaatg tggtacaact
     4081 gcgtgaggtg ttcacgacgg gtagtagtgg ttctttagta ttagtgtacg actatcatcc
     4141 cggctcacaa acattactgg ccaaatactt cactccggcg ccagagacca acgggtacac
     4201 tgatccattc caaggcgagg cccgcccctt cagtcataag agtaacatgc agcgaacgag
     4261 caatggaccg ctgctaccgg aggccaccat ctggtcgatc atcatgcagc tgaccgccgg
     4321 cttaaaagcc atccaccatg ccggtcttgc ctgcaaggtt ctggatccca caaagattat
     4381 agtgacgggc aagcgtgtac ggtttagctc atgctgcatt tcggatataa cgcaattcga
     4441 tccgaatgca tccaatccct tggcgctggt taatatgcat cagcaggacg atctgaccgc
     4501 tttgggtcgc ttagtattag ctttggcctg ccgctgtctt caatccgtgc aacgcgacaa
     4561 tgtccagtcc agcatcgata tggtcacgcg aaactactcc accgatctgc gaaactttat
     4621 tgtttacctc ttcaccacca ataatcgtcg ctccgtgacc gacctgatgc cgatgattgg
     4681 tgcccgcttc tatacgcaac tggatgcact gcagagcaaa attgatatgc aggaggacga
     4741 ggaagccaag gagatggaga acgagcggtt atatcgcatt ttggtcaaac ttaatagcat
     4801 caacgagcga cccgacttta atctggactg cacttggtct gagactggtg atagatacat
     4861 gttgaaatta ttccgtgatt atttgttcca ttccgtcact gaggatggcc ggccctggct
     4921 agatcacgca cacattgtac aatgcctcaa caagctggat gctggctcca tagagcgcgt
     4981 gcaactgatg tcccgcgacg agcaatcggt actcatcgtc tcctatgctg aactcaagaa
     5041 ttgtctggag aacgccttct ccgaactgat gtcaagtgcg gccaactgag cggccgctcg
     5101 agtctagagg gcccgcggtt cgaaggtaag cctatcccta accctctcct cggtctcgat
     5161 tctacgcgta ccggtcatca tcaccatcac cattgagttt aaacccgctg atcagcctcg
     5221 actgtgcctt ctaaggcctg agctcgctga tcagcctcga tcgaggatcc agacatgata
     5281 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
     5341 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
     5401 aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt
     5461 taaagcaagt aaaacctcta caaatgtggt atggctgatt atgatcagtc gaccgatgcc
     5521 cttgagagcc ttcaacccag tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc
     5581 cgcacttatg actgtcttct ttatcatgca actcgtagga caggtgccgg cagcgctctg
     5641 ggtcattttc ggcgaggacc gctttcgctg gagcgcgacg atgatcggcc tgtcgcttgc
     5701 ggtattcgga atcttgcacg ccctcgctca agccttcgtc actggtcccg ccaccaaacg
     5761 tttcggcgag aagcaggcca ttatcgccgg catggcggcc gacgcgctgg gctacgtctt
     5821 gctggcgttc gcgacgcgag gctggatggc cttccccatt atgattcttc tcgcttccgg
     5881 cggcatcggg atgcccgcgt tgcaggccat gctgtccagg caggtagatg acgaccatca
     5941 gggacagctt caaggatcgc tcgcggctct taccagccag caaaaggcca ggaaccgtaa
     6001 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     6061 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     6121 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     6181 cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag
     6241 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     6301 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     6361 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     6421 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
     6481 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     6541 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     6601 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     6661 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     6721 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     6781 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     6841 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     6901 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     6961 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     7021 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     7081 cgttgttgcc attgctgcag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     7141 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     7201 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     7261 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     7321 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     7381 ctcttgcccg gcgtcaacac gggataatac cgcgccacat agcagaactt taaaagtgct
     7441 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     7501 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     7561 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     7621 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     7681 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     7741 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac
     7801 attaacctat aaaaataggc gtatcacgag gccctttcgt
//
