LOCUS       sequence-290203-        7632 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmPABPC1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7632)
  AUTHORS   Zekri L, Kuzuoglu-Ozturk D, Izaurralde E
  TITLE     GW182 proteins cause PABP dissociation from silenced miRNA targets 
            in the absence of deadenylation.
  JOURNAL   EMBO J. 2013 Apr 3;32(7):1052-65. doi: 10.1038/emboj.2013.44. Epub 
            2013 Mar 5.
  PUBMED    23463101
REFERENCE   2  (bases 1 to 7632)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/emboj.2013.44"; journalName: "EMBO J"; date: "2013-04-3- 
            3"; volume: "32"; issue: "7"; pages: "1052-65"
COMMENT     Sequence Label: pAc5.1B-MS2-DmPABPC1-V5His6_V
FEATURES             Location/Qualifiers
     source          1..7632
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             4917..4958
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4968..4985
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5008..5025)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5058..5192
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5082..5101)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5136..5155
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(5814..6402)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5894..5913)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6573..7364,7365..7433))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7196..7215
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7434..7538)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7606..7624
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 tacccaacat ggcttctaac tttactcagt tcgttctcgt cgacaatggc ggaactggcg
     2641 acgtgactgt cgccccaagc aacttcgcta acggggtcgc tgaatggatc agctctaact
     2701 cgcgatcaca ggcttacaaa gtaacctgta gcgttcgtca gagctctgcg cagaatcgca
     2761 aatacaccat caaagtcgag gtgcctaaag tggcaaccca gactgttggt ggtgaagagc
     2821 ttcctgtagc cggatggaga tcttacttaa atatggaact aaccattcca attttcgcca
     2881 cgaattccga ctgcgagctt attgttaagg caatgcaagg tctcctaaaa gatggaaacc
     2941 cgattccctc ggccatcgcg gccaactccg gcatctaccc aagcttgatg gcttctctat
     3001 acgtcggtga tctcccacag gacgtcaacg aatcgggact ttttgacaag ttctcgtctg
     3061 ctggtccagt gctgtccatt cgtgtctgcc gcgatgtgat tacccgtcgc tcgttgggct
     3121 atgcctacgt caacttccag cagccagccg atgctgagcg tgctttggac accatgaact
     3181 ttgacctggt tcgcaacaag cccattcgca ttatgtggtc tcagcgtgat ccgtctcttc
     3241 gtcgctccgg tgtgggcaac gtgttcatca agaacttaga tagggccatc gacaacaagg
     3301 caatctacga cactttctcc gccttcggca acattttaag ctgcaaggta gccaccgatg
     3361 aaaagggcaa ctcaaagggc tatggattcg tccacttcga gaccgaggag gccgcaaaca
     3421 cgtccatcga caaagtcaat ggcatgttgc ttaacggcaa gaaggtctac gtgggtaagt
     3481 tcatcccgcg caaggagcgc gagaaggagc tgggcgagaa ggctaagctc ttcaccaatg
     3541 tatatgtgaa gaacttcact gaggatttcg acgatgaaaa actgaaggaa ttctttgagc
     3601 cctacggcaa gataaccagc tacaaggtca tgtccaagga ggatggcaag agcaagggct
     3661 tcggattcgt tgcattcgag acaacagagg ctgctgaggc ggccgttcag gctctcaatg
     3721 gcaaggacat gggagagggc aagtccctgt acgtggctcg tgcccagaag aaggccgaac
     3781 gccagcagga gctgaagcgc aagttcgagg aactgaagca gaagcgtcac gagtcagtgt
     3841 tcggcgttaa cttgtacgtt aagaacctgg acgacacaat cgatgacgat cgtctgcgca
     3901 tcgcgttctc tccatacggc aacatcacat cggccaaggt catgaccgat gaggagggtc
     3961 gctccaaggg attcggcttc gtgtgcttca atgccgcaag cgaggctacc tgcgccgtca
     4021 ccgagctgaa cggtcgcgtc gtcggcagta agccgttgta cgttgctttg gcccaaagga
     4081 aggaggagcg caaggctcat ctcgcctcgc agtacatgcg tcacatgacc ggcatgcgta
     4141 tgcagcagct gggacagatc taccagccca acgcggctag cggcttcttt gtgccgaccc
     4201 ttccgtcaaa tcagcgtttc ttcggttccc aggtggccac tcagatgaga aacacacccc
     4261 gctgggtgcc ccaggtgcgt cccccagcag ccatacaggg tgtccaggcc ggagctgccg
     4321 ctgctggtgg cttccagggc acagccggtg cggtgcccac tcagttccgt tcggctgctg
     4381 ctggcgctcg cggggcacag cctcaggtgc agggcacaca cgccgccgct gctgcggcta
     4441 acaacatgcg caacactgga gcacgcgcta ttactggcca gcaaactgca gcacccaaca
     4501 tgcagatccc cggagcccaa atcgctggcg gtgcccagca gcgtacttcc aactataagt
     4561 acacctcaaa catgcgtaat cctccagtgc cacagttgca tcagacccag ccaattccac
     4621 agcaattgca aggaaagaat tctgagaagc tcattgcctc gttgctggcc aacgccaagc
     4681 cgcaggagca gaaacagatc ctgggcgagc gtctgtatcc gatgattgaa catatgcacg
     4741 ccaatttggc tggtaagatc accggcatgt tgttggagat cgaaaactct gagctcttgc
     4801 acatgatcga ggatcaggag gcccttaagg ccaaggtgga ggaggctgtg gccgtgcttc
     4861 aggtgcaccg cgtcaccgag cccgccaact cgagtctaga gggcccgcgg ttcgaaggta
     4921 agcctatccc taaccctctc ctcggtctcg attctacgcg taccggtcat catcaccatc
     4981 accattgagt ttaaacccgc tgatcagcct cgactgtgcc ttctaaggcc tgagctcgct
     5041 gatcagcctc gatcgaggat ccagacatga taagatacat tgatgagttt ggacaaacca
     5101 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
     5161 ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt
     5221 ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg
     5281 gtatggctga ttatgatcag tcgaccgatg cccttgagag ccttcaaccc agtcagctcc
     5341 ttccggtggg cgcggggcat gactatcgtc gccgcactta tgactgtctt ctttatcatg
     5401 caactcgtag gacaggtgcc ggcagcgctc tgggtcattt tcggcgagga ccgctttcgc
     5461 tggagcgcga cgatgatcgg cctgtcgctt gcggtattcg gaatcttgca cgccctcgct
     5521 caagccttcg tcactggtcc cgccaccaaa cgtttcggcg agaagcaggc cattatcgcc
     5581 ggcatggcgg ccgacgcgct gggctacgtc ttgctggcgt tcgcgacgcg aggctggatg
     5641 gccttcccca ttatgattct tctcgcttcc ggcggcatcg ggatgcccgc gttgcaggcc
     5701 atgctgtcca ggcaggtaga tgacgaccat cagggacagc ttcaaggatc gctcgcggct
     5761 cttaccagcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     5821 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     5881 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     5941 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     6001 ctttctcaat gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     6061 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     6121 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     6181 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     6241 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     6301 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     6361 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     6421 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     6481 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     6541 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     6601 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     6661 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     6721 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     6781 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     6841 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctgc aggcatcgtg
     6901 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     6961 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     7021 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     7081 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     7141 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac acgggataat
     7201 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     7261 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     7321 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     7381 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     7441 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     7501 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     7561 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     7621 aggccctttc gt
//
