LOCUS       sequence-290284-       11886 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of HsNot1iso1-IKGIRQtoDSYDAA-shRNAres.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11886)
  AUTHORS   Chen Y, Boland A, Kuzuoglu-Ozturk D, Bawankar P, Loh B, Chang CT, 
            Weichenrieder O, Izaurralde E
  TITLE     A DDX6-CNOT1 complex and W-binding pockets in CNOT9 reveal direct 
            links between miRNA target recognition and silencing.
  JOURNAL   Mol Cell. 2014 Jun 5;54(5):737-50. doi: 
            10.1016/j.molcel.2014.03.034. Epub 2014 Apr 24.
  PUBMED    24768540
REFERENCE   2  (bases 1 to 11886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2014.03.034"; journalName: "Mol Cell"; date: 
            "2014-06-5- 5"; volume: "54"; issue: "5"; pages: "737-50"
COMMENT     Sequence Label: pT7-EGFP-C1-HsNot1iso1-IKGIRQtoDSYDAA-shRNAres_W
FEATURES             Location/Qualifiers
     source          1..11886
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     613..632
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        613..631
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      659..668
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(665..667,668..670,671..1381)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(665..667,668..670,671..1381)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(710..731)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(971..990)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1318..1339
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    8674..8795
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8711..8730)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8765..8784
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(8802..9257)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8939..8960)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9151..9170
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        9284..9388
                     /gene="bla"
                     /label=AmpR promoter
     promoter        9390..9747
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        9390..9581
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(9413..9433)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      9598..9733
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9782..10576
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             9782..10576
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9836..9855)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10446..10465
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(10764..10783)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    10808..10855
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      11184..11772
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11673..11692
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca ctgcttactg
      601 gcttatcgaa attaatacga ctcactatag ggagacccaa gctctagcgc taccggtcgc
      661 caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
      721 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
      781 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
      841 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
      901 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
      961 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     1021 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     1081 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
     1141 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     1201 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     1261 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
     1321 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     1381 gtccggactc agatctcgag ctatgaatct tgactcgctc tcgctggcct tgtctcaaat
     1441 cagctacctg gtggacaatt taaccaagaa aaattaccga gccagccagc aggaaataca
     1501 gcatattgtg aatcggcacg gtcctgaggc agacaggcat ttattacgct gcctattttc
     1561 gcatgtggat ttcagtggcg atggtaaaag cagtggcaaa gatttccatc agactcagtt
     1621 tctgattcag gagtgtgcgt tgctgattac aaagccaaat tttatctcga cgctgtccta
     1681 tgccattgat aatccattgc actatcagaa gagtttaaag cctgcacccc acttatttgc
     1741 ccagctgagt aaagtgctca aattaagcaa agtacaagag gtaatttttg gccttgccct
     1801 gttgaattct tccagctcag atcttagagg tttcgctgcc cagtttatca aacagaagct
     1861 tccagatctt ctgcgttctt acattgacgc agacgtcagt ggaaatcaag aaggtggctt
     1921 ccaagatata gcaatagagg tcctacacct cctcctctcc catctcctct ttgggcagaa
     1981 gggagccttt ggagttggac aagaacagat agacgctttt cttaagacgc tgcgcagaga
     2041 ttttccccaa gaacgctgtc ccgtggtgct cgcaccactt ttataccctg aaaaacggga
     2101 cattctaatg gacaggatcc tgcctgattc cggaggggta gctaaaacca tgatggagag
     2161 ctctttggct gatttcatgc aagaagtagg ctatggcttt tgtgcaagta ttgaagaatg
     2221 tcgcaatata atcgtgcagt ttggtgttcg ggaggtcaca gctgcccagg ttgcaagggt
     2281 tttgggaatg atggctcgaa ctcattcagg attaacagat ggcattccat tacagagtat
     2341 ttctgctccg ggcagtggga tctggagtga tgggaaagat aaaagtgatg gagcacaggc
     2401 acacacatgg aatgtagaag tcttgattga cgttcttaaa gaactgaatc caagtttgaa
     2461 tttcaaggaa gtaacttatg aactggacca tcctggattt caaattcgtg acagtaaagg
     2521 acttcataat gtggtttatg gcattcagag gggtttgggt atggaagtgt tcccagtaga
     2581 cctcatatat agaccttgga aacatgctga aggccagctc tccttcatcc agcactcact
     2641 aatcaatcca gagatcttct gttttgctga ctatccctgt catactgttg ccactgatat
     2701 tctgaaagca ccaccagagg atgacaatcg agaaattgcc acatggaaga gcttggattt
     2761 gattgaatct ctgctgaggc ttgcagaggt tgggcagtat gagcaagtca aacagctctt
     2821 cagcttccct atcaaacact gtccagacat gctggtattg gccttactac aaattaacac
     2881 ctcttggcat accttgcgcc atgaacttat ctccactctg atgccaattt tccttggaaa
     2941 ccatcctaac tcagctatta ttttgcacta tgcatggcat gggcagggac agtctccctc
     3001 aattcgccaa cttatcatgc atgcaatggc agaatggtac atgagagggg agcagtatga
     3061 tcaggccaaa ttgtctcgaa tacttgatgt ggcccaggac ttgaaggcct tgtcaatgct
     3121 gctaaatggt actccatttg cctttgttat tgaccttgct gcacttgctt cacgtcgtga
     3181 atacctcaaa cttgataagt ggctcacaga taaaattcga gagcatgggg agccttttat
     3241 ccaggcgtgt atgacttttt taaagagacg gtgtccttct attttgggcg gacttgcccc
     3301 agaaaaagac cagcccaaaa gtgctcaact tcctccagaa actttggcga caatgttggc
     3361 ctgtctgcaa gcttgtgcag ggagtgtttc tcaggagcta tcagaaacta tcctcaccat
     3421 ggtagccaat tgcagtaatg ttatgaataa ggccagacaa ccaccacctg gagttatgcc
     3481 aaaaggacgt cctcctagtg ctagcagctt agatgccatt tctcctgttc agattgaccc
     3541 tcttgctgga atgacatctc ttagtatagg tggttcagct gcccctcaca cccagagtat
     3601 gcagggtttt cctccaaatt tgggttctgc attcagtacc cctcagtcac cagcaaaagc
     3661 atttccaccc ctttcaaccc ccaatcagac cactgcattc agtggtattg gaggactttc
     3721 atcacagctt ccagtaggtg gtcttggcac aggcagcctg actggtatag gaactggtgc
     3781 tcttggactc cctgcagtga ataacgaccc ttttgtacag aggaaactgg gcacctctgg
     3841 actgaatcag cctacattcc agcagagtaa gatgaaacct tcggacttgt ctcaggtgtg
     3901 gccagaggca aaccagcact ttagtaaaga gatagatgat gaagcaaaca gctatttcca
     3961 gcgaatatat aatcatccac cacatccaac catgtctgtt gatgaggtat tagaaatgct
     4021 gcagagattt aaagactcta ctataaagag ggaacgagaa gtatttaact gtatgctaag
     4081 gaacttgttt gaagaatatc gtttttttcc ccagtatcct gataaagagt tacatataac
     4141 agcctgccta tttggtggta taattgagaa aggactggtc acttacatgg cactaggtct
     4201 ggctctacga tatgttcttg aagccttacg caagcctttt ggatccaaaa tgtattattt
     4261 cgggattgct gcactagata gatttaaaaa cagattgaag gactatcccc agtattgtca
     4321 gcatttggct tctatcagtc actttatgca atttccacat catttacagg agtatattga
     4381 gtatggacag cagtctagag atcctcctgt gaaaatgcaa ggctctatca caacccctgg
     4441 aagtattgca ctggctcagg cccaggctca ggcccaggtt ccagcaaaag ctcctcttgc
     4501 tggtcaagtt agcactatgg taaccacctc aacaactacc actgttgcta aaacggttac
     4561 ggtcaccagg ccaactggag tcagctttaa gaaagatgtg ccaccttcta ttaatactac
     4621 aaatatagat acgttgcttg tggccacaga tcaaactgag agaattgtgg agcccccaga
     4681 aaatatccag gagaaaattg cttttatttt caataatctc tcacagtcaa atatgacaca
     4741 aaaggttgaa gagctaaagg aaacggtgaa agaagaattt atgccttggg tttcacagta
     4801 tctggttatg aagagagtca gtattgagcc aaactttcat agcctgtatt caaacttcct
     4861 tgacacgctg aagaatcctg aatttaacaa gatggttctg aatgagacct acagaaacat
     4921 taaagtgctc ctgacctctg ataaagctgc agccaatttc tcagatcgtt ctttgctgaa
     4981 gaacttggga cattggctag gaatgatcac attagctaaa aacaaaccca tcttacacac
     5041 tgacttggat gtgaaatcat tgctgctaga ggcttatgtt aaaggacaac aagaattgct
     5101 ctatgtagtg ccctttgttg ccaaagtctt agaatctagc attcgtagtg tggtttttag
     5161 gccaccaaac ccttggacaa tggcaattat gaatgtatta gctgagctac atcaggagca
     5221 tgacttaaag ttaaacttga agtttgaaat cgaggttctc tgcaagaacc ttgcattaga
     5281 catcaatgag ctaaaacctg gaaacctcct aaaggataaa gatcgcctga agaatttaga
     5341 tgagcaactc tctgctccaa agaaagatgt caagcagcca gaagaactcc ctcccatcac
     5401 aaccacaaca acttctacta caccagctac caacaccact tgtacagcca cggttccacc
     5461 acagccacag tacagctacc acgacatcaa tgtctattcc cttgcgggct tggcaccaca
     5521 cattactcta aatccaacaa ttcccttgtt tcaggcccat ccacagttga agcagtgtgt
     5581 gcgtcaggca attgaacggg ctgtccagga gctggtccat cctgtggtgg atcgatcaat
     5641 taagattgcc atgactactt gtgagcaaga tgtcaggtcg gattttgccc tggattcgga
     5701 ggaatctcga atgcgaatag cagctcatca catgatgcgt aacttgacag cttacatggc
     5761 tatggataca tgcgctgaac ctttgctcat gagcatatct accaacttaa aaaacagttt
     5821 tgcctcagcc cttcgtactg cttccccaca acaaagagaa atgatggatc aggcagctgc
     5881 tcaattagct caggacaatt gtgagttggc ttgctgtttt attcagaaga ctgcagtaga
     5941 aaaagcaggc cctgagatgg acaagagatt agcaactgaa tttgagctga gaaaacatgc
     6001 taggcaagaa ggacgcagat actgtgatcc tgttgtttta acatatgctg ctgaacggat
     6061 gccagagcaa atcaggctga aagttggtgg tgtggaccca aagcagttgg ctgtttacga
     6121 agagtttgca cgcaatgttc ctggcttctt acctacaaat gacttaagtc agcccacggg
     6181 atttttagcc cagcccatga agcaagcttg ggcaacagat gatgtagctc agatttatga
     6241 taagtgtatt acagaactgg agcaacatct acatgccatc ccaccaactt tggccatgaa
     6301 ccctcaagct caggctcttc gaagtctctt ggaggttgta gttttatctc gaaactctcg
     6361 ggatgccata gctgctcttg gattgctcca aaaggctgta gagggcttac tagatgccac
     6421 aagtggtgct gatgctgacc ttctgctgcg ctacagggaa tgccacctct tggtcctaaa
     6481 agctctgcag gatggccggg catatgggtc tccatggtgc aacaaacaga tcacaaggtg
     6541 cctaattgaa tgtcgagatg aatataaata taatgtggag gctgtggagc tgctaattcg
     6601 caatcatttg gttaatatgc agcagtatga tcttcaccta gcgcagtcaa tggagaatgg
     6661 cttaaactac atggctgtgg catttgctat gcagttagta aaaatcctgc tggtggatga
     6721 aaggagtgtt gctcatgtta ctgaggcaga tctgttccac accattgaaa ccctcatgag
     6781 gattaatgct cattccagag gcaatgctcc agaaggattg ccccagctga tggaagtagt
     6841 gcgatccaac tatgaagcaa tgattgatcg tgctcatgga ggcccaaact ttatgatgca
     6901 ttctgggatc tctcaagcct cagagtatga tgaccctcca ggcctgaggg agaaggcaga
     6961 gtatcttctg agggaatggg tgaatctcta ccattcagca gcagctggcc gcgacagtac
     7021 caaagctttc tctgcatttg ttggacagat gcaccagcaa ggaatactga agaccgatga
     7081 tctcataaca aggttctttc gtctgtgtac tgaaatgtgt gttgaaatca gttaccgtgc
     7141 tcaggctgag cagcagcaca atcctgctgc caatcccacc atgatccgag ccaagtgcta
     7201 tcacaacctg gatgcctttg ttcgactcat tgcactgctc gtgaaacact caggggaggc
     7261 caccaacact gtcacaaaga ttaatctgct gaacaaggtc cttggtatag tagtgggagt
     7321 tctccttcag gatcatgatg ttcgtcagag tgaatttcag caacttccct accatcgaat
     7381 ttttatcatg cttctcttgg aactcaatgc acctgagcat gtgttggaaa ccattaattt
     7441 ccagacactt acagctttct gcaatacatt ccacatcttg aggcctacca aagctcctgg
     7501 ctttgtatat gcctggcttg aactgatttc ccatcggata tttattgcaa gaatgctggc
     7561 acatacgcca cagcagaagg ggtggcctat gtatgcacag ctactgattg atttattcaa
     7621 atatttagcg cctttcctta gaaatgtgga actcaccaaa cctatgcaaa tcctctacaa
     7681 gggcacttta agagtgctgc tggttctttt gcatgatttc ccagagttcc tttgtgatta
     7741 ccattatggg ttctgtgatg tgatcccacc taattgtatc cagttaagaa atttgatcct
     7801 gagtgccttt ccaagaaaca tgaggctccc cgacccattc actcctaatc taaaggtgga
     7861 catgttgagt gaaattaaca ttgctccccg gattctcacc aatttcactg gagtaatgcc
     7921 acctcagttc aaaaaggatt tggattccta tcttaaaact cgatcaccag tcactttcct
     7981 gtctgatctg cgcagcaacc tacaggtatc caatgaacct gggaatcgct acaacctcca
     8041 gctcatcaat gcactggtgc tctatgtcgg gactcaggcc attgcgcaca tccacaacaa
     8101 gggcagcaca ccttcaatga gcaccatcac tcactcagca cacatggata tcttccagaa
     8161 tttggctgtg gacttggaca ctgagggtcg ctatctcttt ttgaatgcaa ttgcaaatca
     8221 gctccggtac ccaaatagcc acactcacta cttcagttgc accatgctgt acctttttgc
     8281 agaggccaat acggaagcca tccaagaaca gatcacaaga gttctcttgg aacggttgat
     8341 tgtaaatagg ccacatcctt ggggtcttct tattaccttc attgagctga ttaaaaaccc
     8401 agcgtttaag ttctggaacc atgaatttgt acactgtgcc ccagaaatcg aaaagttatt
     8461 ccagtcggtc gcacagtgct gcatgggaca gaagcaggcc cagcaagtaa tggaagggac
     8521 aggtgccagt tagccgcggg cccgggatcc accggatcta gataactgat cataatcagc
     8581 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
     8641 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
     8701 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     8761 agttgtggtt tgtccaaact catcaatgta tcttaacgcg taaattgtaa gcgttaatat
     8821 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga
     8881 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc
     8941 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac
     9001 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc
     9061 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg
     9121 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag
     9181 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc
     9241 gccgctacag ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     9301 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     9361 gcttcaataa tattgaaaaa ggaagagtcc tgaggcggaa agaaccagct gtggaatgtg
     9421 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     9481 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     9541 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     9601 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     9661 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     9721 ttttttggag gcctaggctt ttgcaaagat cgatcaagag acaggatgag gatcgtttcg
     9781 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
     9841 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
     9901 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
     9961 gcaagacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
    10021 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
    10081 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
    10141 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
    10201 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
    10261 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcga gcatgcccga
    10321 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
    10381 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
    10441 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
    10501 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
    10561 tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac
    10621 ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
    10681 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
    10741 gcccacccta gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc
    10801 gctatgacgg caataaaaag acagaataaa acgcacggtg ttgggtcgtt tgttcataaa
    10861 cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc ccattggggc
    10921 caatacgccc gcgtttcttc cttttcccca ccccaccccc caagttcggg tgaaggccca
    10981 gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag cctcaggtta ctcatatata
    11041 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
    11101 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
    11161 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
    11221 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
    11281 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
    11341 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
    11401 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
    11461 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
    11521 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
    11581 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
    11641 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
    11701 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
    11761 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
    11821 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
    11881 atgcat
//
