LOCUS       sequence-290364-        7152 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmRUMP_1-563.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7152)
  TITLE     Izaurralde lab plasmids
REFERENCE   2  (bases 1 to 7152)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmRUMP_1-563_X
FEATURES             Location/Qualifiers
     source          1..7152
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2588..2653
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2678..2704
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4437..4478
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4488..4505
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4528..4545)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4578..4712
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4602..4621)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4656..4675
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(5334..5922)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5414..5433)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6093..6884,6885..6953))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6716..6735
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6954..7058)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7126..7144
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gacgcacaaa cacgacgacg tgagcgtcgc gctgagaaac aagctcaatg
     2641 gaaagctgca aacggtggat ctcctccaca tatggcttac ccatacgatg ttccagatta
     2701 cgctcctcca agcttgatga gcatggacgc tagtaactcg gtggagagcc gcgagaagga
     2761 gcgcgaccgt cgtggacgcg gagcacgtgg ctcccgtttc acggacgctg atggcaatgg
     2821 aaacggcgct ggtagtcagg gaggtggcgt cgctgctcgc gaccgtagcc gggaacgtcg
     2881 caattgccgg gtctacattt cgaacattcc atacgactac cgttggcagg atctgaagga
     2941 tctgttccgc cgcatcgtcg gctccattga gtacgtccag ctgttcttcg atgagagcgg
     3001 caaggctcgc ggctgtggca tcgtagagtt caaggatccg gagaacgtac agaaggcctt
     3061 ggagaaaatg aaccgctatg aggtgaatgg ccgcgaactg gtggtcaagg aggatcacgg
     3121 cgagcagcgc gatcaatacg gacgcattgt gcgagatggt ggtggtggtg gaggcggcgg
     3181 tggcggcgta caaggaggca atggtggcaa caatggagga ggtggcggcg gtggccgtga
     3241 ccacatggat gaccgcgatc ggggtttctc ccggcgagac gacgacagac tatctgggcg
     3301 taataatttt aacatgatgt caaatgatta taataattcg tcgaattaca atttgtatgg
     3361 gctttctgct tcgtttttgg agagtcttgg cataagtgga cctttgcata ataaggtttt
     3421 tgttgctaat ctggactaca aggtcgacaa caagaagctc aagcaggtct tcaagctggc
     3481 cggcaaggtg cagagcgtag acctttcact ggacaaggag ggcaacagcc gcggttttgc
     3541 tgtgatcgag tacgaccatc cggtggaggc tgttcaggcc atttccatgc tggatcgtca
     3601 gatgctattc gaccgtcgta tgaccgtgcg cctggaccga attccggata agaatgaagg
     3661 cataaagctg cccgagggct tgggaggtgt gggcatcggc ctgggaccca acggcgagcc
     3721 actcagggac gtggctcaca accttcccaa tggtggtcag agccagggcc agttgcttgg
     3781 caacgctcag cagggatctc agctgggatc ggtgggtagc caaccaaaca gcagcgctgt
     3841 tagcaacgcc acgacaaacc tgcttaacaa cctgaccggc gttatgtttg gcaatcatgc
     3901 ggcagtgcag ccatcgcctg tagctccggt gcaaaagcct agcctaggaa acaataccgg
     3961 ttccggcgga ttgaacctaa acaatctgaa tcccagcatt ttggctgccg tggtcggcaa
     4021 tcttggtaac cagggaggga atttaagcaa tcccttgctg tcctcttcgc tgagcaacct
     4081 gggtctcaat ctgggcaact ccggcaatga tgacaacctg ccgccaagca atgtgggtct
     4141 ttcaaacaac tacagcagcg gcggcaccgg tggtggcaat agctatagct ctggaaacaa
     4201 ctacagcggc ggtggcggct ccagcaattt gggatacaat gcttacagca gctctggtgg
     4261 aatgggcggc ggcaacggag gagttggtgt tgatggcaac gattacaaca ctggcaatcc
     4321 attggatgtc tatggcggcg gcagcaacgt gggcaattcc aacgttggct cggccaatgc
     4381 tgtcggtgct tctcgaaagt cggactaact cgagtctaga gggcccgcgg ttcgaaggta
     4441 agcctatccc taaccctctc ctcggtctcg attctacgcg taccggtcat catcaccatc
     4501 accattgagt ttaaacccgc tgatcagcct cgactgtgcc ttctaaggcc tgagctcgct
     4561 gatcagcctc gatcgaggat ccagacatga taagatacat tgatgagttt ggacaaacca
     4621 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
     4681 ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt
     4741 ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg
     4801 gtatggctga ttatgatcag tcgaccgatg cccttgagag ccttcaaccc agtcagctcc
     4861 ttccggtggg cgcggggcat gactatcgtc gccgcactta tgactgtctt ctttatcatg
     4921 caactcgtag gacaggtgcc ggcagcgctc tgggtcattt tcggcgagga ccgctttcgc
     4981 tggagcgcga cgatgatcgg cctgtcgctt gcggtattcg gaatcttgca cgccctcgct
     5041 caagccttcg tcactggtcc cgccaccaaa cgtttcggcg agaagcaggc cattatcgcc
     5101 ggcatggcgg ccgacgcgct gggctacgtc ttgctggcgt tcgcgacgcg aggctggatg
     5161 gccttcccca ttatgattct tctcgcttcc ggcggcatcg ggatgcccgc gttgcaggcc
     5221 atgctgtcca ggcaggtaga tgacgaccat cagggacagc ttcaaggatc gctcgcggct
     5281 cttaccagcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     5341 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     5401 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     5461 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     5521 ctttctcaat gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     5581 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     5641 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     5701 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     5761 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     5821 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     5881 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     5941 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     6001 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     6061 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     6121 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     6181 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     6241 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     6301 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     6361 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctgc aggcatcgtg
     6421 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     6481 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     6541 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     6601 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     6661 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac acgggataat
     6721 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     6781 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     6841 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     6901 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     6961 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     7021 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     7081 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     7141 aggccctttc gt
//
