LOCUS       sequence-290508-        8042 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmPAN2_377-1241.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8042)
  AUTHORS   Jonas S, Christie M, Peter D, Bhandari D, Loh B, Huntzinger E, 
            Weichenrieder O, Izaurralde E
  TITLE     An asymmetric PAN3 dimer recruits a single PAN2 exonuclease to 
            mediate mRNA deadenylation and decay.
  JOURNAL   Nat Struct Mol Biol. 2014 Jul;21(7):599-608. doi: 10.1038/nsmb.2837.
            Epub 2014 Jun 1.
  PUBMED    24880343
REFERENCE   2  (bases 1 to 8042)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.2837";
            journalName: "Nat Struct Mol Biol"; date: "2014-07"; volume: "21"; 
            issue: "7"; pages: "599-608"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmPAN2_377-1241_Y
FEATURES             Location/Qualifiers
     source          1..8042
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2588..2653
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2678..2704
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5327..5368
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5378..5395
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5418..5435)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5468..5602
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5492..5511)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5546..5565
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(6224..6812)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6304..6323)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6983..7774,7775..7843))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7606..7625
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7844..7948)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8016..8034
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gacgcacaaa cacgacgacg tgagcgtcgc gctgagaaac aagctcaatg
     2641 gaaagctgca aacggtggat ctcctccaca tatggcttac ccatacgatg ttccagatta
     2701 cgctcctcca agcttgatta cggacaccaa ctttccgcta tcgtcggtca tgctaccgca
     2761 cttgacgacg ggcacacaat ggttctcaga ttggccggag gaactgctgc gctaccgcta
     2821 ccacaggccc aagaccattg atcccgaagt gctgagcaac atgaaaatgc agggaccgat
     2881 tggatactcg ccaaacccac gcaccgcccg tcgtaatcaa attccgtacg tgattgagca
     2941 gggcggcgtg tgcagtccga acggaaatgg aactgcggcg gcgaccaagg cggagaacgg
     3001 tgtgaaaatc attccacggc ggtatcgcaa agtcgagctg aagtacacga agctgggcac
     3061 ccaggacttt gactttgacc agcataatca aacatgtttc gcggggctgg aggcaacgct
     3121 accgaactcc tactgcaatg ctatgctaca gattttgtat ttcaccgacg cacttcgcgt
     3181 gaagctgctg gagcattcgt gcatcaagga gttttgcctg tcctgcgaac tagggttcct
     3241 cttcaacatg ctggataaaa gtaccgcttc ttcaccttgc caggccagca atttcctgcg
     3301 atctttccgc actgtgccag aggcctcggc actgggattg attctcaccg accgctcatc
     3361 gaacgtcaat ctaatatcgc tgatacagaa ctggaaccga ttcattttgc atcaaatgca
     3421 ttacgaaata ttcgattcta gcaagaacgc atccacctac agcggatctg tgcaaacatc
     3481 aacgaacgcg gaaaacgcgg gctcttcaga aacaagtggc tcctcggacc tttacgattc
     3541 cataagtgat gagaactcca aggaggacga tcgggaacgc agtaagatca acgcagaaac
     3601 tgacatcagt aagatctttg ggaccaagca aatctgtata aaccgctgca tcaaatgcca
     3661 agaagagaag ataaaggaaa gcattctact tgcctgcaat ttgtcgtatc ccaaccacat
     3721 caaagatagt gatcagtatt tcaactttgg caccattttg aagcgatctc tcagctccga
     3781 gaagagtatt caggccttct gcgagagatg taagaagttt tcgccaacca atcagagcgt
     3841 gaaggtgact tcgcttccgc aaattctgtc cattaactgt ggcctcaaca acgaaaaaga
     3901 cattaccttc ctgaagcgcc agctcaatcg ctgcagcgaa aagacgactg ttgatgcggc
     3961 cgcttccttg agcacaagca agccgtgtcg atatggagcc aactgttcgc gaagtgactg
     4021 ccatttcatg catccggatc gaaaatctcc atcgcacaca agccaaccga atgctgtcaa
     4081 caattctccc aatggacgcc aaaagtcctg gtttcccctt acatttacta tgggcatcaa
     4141 tgaccaaggt gaggtgcagg tgcaaactca atcagatgca agcagtggca aatcggaaca
     4201 ggaggaggaa acggaaaagc caccaacaaa gggcttggac aacaatcgga tgtacgcgct
     4261 gcatgccgtg gtgtgccaag tcgatgatgg tacccaaaag aacttggtat cgttgataaa
     4321 tgtgcagcgg ccataccaca ccatgaaact ggctgaatct gcagatgacc ctcagagtca
     4381 atggtatatt tttaatgatt ttagcatatc gcccgtttcg ccacaagaat cggtatggtt
     4441 cacattggat tggaaagttc cttgcatact cttctacaga cacgtagagg atgactctga
     4501 gtctgctagt actactagct cgaccgtcac ggaaagtgaa gaaaccattc catcagagag
     4561 cagcagcggc tcgccaacaa atctatccaa cccttttcta gaggaaattg tgagtcctat
     4621 gctaggcaac ttaagtgcag acgccacgct gcagcccctg cagtcagatg aaatgccgca
     4681 gtcgggtgat ctggtcgcaa tggatgccga gttcgttacc ctaaatccag aggaaaatga
     4741 gattcgcccg gatggcaaaa cggcgaccat taagccatgc cacatgagtg tggcccggat
     4801 ttcatgtata cgaggacaag gcccggctga aggagttcca ttcatggacg attacatatc
     4861 cacccaggag aaggtcgtgg actacctaac tcagttctcg ggaatcaagc ctggcgactt
     4921 ggacgctaac ttcagcaaaa agcgactgac tgccttgaag tactcatacc agaaattaaa
     4981 atacctcgtt gacgtaggag taatatttgt cggacatggt ctgaagaacg actttagggt
     5041 cataaacatc tatgtaccgt cggagcagat aatagatacg gtgcacctgt ttcacatgcc
     5101 acatcatcgc atggtttccc taagatttct agcctggcat ttcttgggta ctaagattca
     5161 gtccgagacg cacgactcca tcgaagatgc acggaccact ctgcagctct acaagcacta
     5221 tttgaagctg caggaggaga agaagtttgc caacgctctc aagaatcttt acgaacgcgg
     5281 aaagcagctg cagtggaagg ttccagagga ctgaccgcgg ttcgaaggta agcctatccc
     5341 taaccctctc ctcggtctcg attctacgcg taccggtcat catcaccatc accattgagt
     5401 ttaaacccgc tgatcagcct cgactgtgcc ttctaaggcc tgagctcgct gatcagcctc
     5461 gatcgaggat ccagacatga taagatacat tgatgagttt ggacaaacca caactagaat
     5521 gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat
     5581 tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca
     5641 gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga
     5701 ttatgatcag tcgaccgatg cccttgagag ccttcaaccc agtcagctcc ttccggtggg
     5761 cgcggggcat gactatcgtc gccgcactta tgactgtctt ctttatcatg caactcgtag
     5821 gacaggtgcc ggcagcgctc tgggtcattt tcggcgagga ccgctttcgc tggagcgcga
     5881 cgatgatcgg cctgtcgctt gcggtattcg gaatcttgca cgccctcgct caagccttcg
     5941 tcactggtcc cgccaccaaa cgtttcggcg agaagcaggc cattatcgcc ggcatggcgg
     6001 ccgacgcgct gggctacgtc ttgctggcgt tcgcgacgcg aggctggatg gccttcccca
     6061 ttatgattct tctcgcttcc ggcggcatcg ggatgcccgc gttgcaggcc atgctgtcca
     6121 ggcaggtaga tgacgaccat cagggacagc ttcaaggatc gctcgcggct cttaccagcc
     6181 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     6241 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     6301 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     6361 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcaat
     6421 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     6481 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     6541 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     6601 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     6661 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     6721 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     6781 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     6841 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     6901 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     6961 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     7021 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     7081 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     7141 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     7201 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     7261 cgccagttaa tagtttgcgc aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct
     7321 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     7381 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     7441 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     7501 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     7561 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac acgggataat accgcgccac
     7621 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     7681 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     7741 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     7801 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     7861 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     7921 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct
     7981 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
     8041 gt
//
