LOCUS       sequence-290516-        9026 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmPAN2-del975-1031GSSG-dsRNAres.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9026)
  AUTHORS   Jonas S, Christie M, Peter D, Bhandari D, Loh B, Huntzinger E, 
            Weichenrieder O, Izaurralde E
  TITLE     An asymmetric PAN3 dimer recruits a single PAN2 exonuclease to 
            mediate mRNA deadenylation and decay.
  JOURNAL   Nat Struct Mol Biol. 2014 Jul;21(7):599-608. doi: 10.1038/nsmb.2837.
            Epub 2014 Jun 1.
  PUBMED    24880343
REFERENCE   2  (bases 1 to 9026)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.2837";
            journalName: "Nat Struct Mol Biol"; date: "2014-07"; volume: "21"; 
            issue: "7"; pages: "599-608"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmPAN2-del975-1031GSSG-dsRN...
FEATURES             Location/Qualifiers
     source          1..9026
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2588..2653
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2678..2704
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6311..6352
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6362..6379
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6402..6419)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6452..6586
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6476..6495)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6530..6549
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(7208..7796)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7288..7307)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7967..8758,8759..8827))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8590..8609
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8828..8932)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9000..9018
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gacgcacaaa cacgacgacg tgagcgtcgc gctgagaaac aagctcaatg
     2641 gaaagctgca aacggtggat ctcctccaca tatggcttac ccatacgatg ttccagatta
     2701 cgctcctcca agcttgaatt ctgcagatat catggactat gtctactgtg gaacggatcc
     2761 gattggagct agcgaggata tcctgtccgt gtacgatgcc ggcagtgccc ccggcaatgg
     2821 tcatttcagc ccgtccttta acggctttaa tattggaacg acggaccccg agtacgtcga
     2881 actggtcccg gtcctggctg atggaggtga acattttgga gtgagctccg tcgcctttga
     2941 tgattatgag gaattgttgt ggatgggaaa tcaaggagga catgtgacca gctattatac
     3001 caatagcatg caaaaatata ccagcttcca agtgcatgct acggatatcg tccgtgatat
     3061 tagcacgttg gatagcggcg tcttggccct gacccaaacg agcttgcgcc accaaattcg
     3121 tcgcggcttg ccgaaattca cgtttaagag caataatatg aaagaaatgg tctccatgtt
     3181 gcaactgtcg ccccaccgct tggtgatggc tggcttgcaa gatgaactga ttgattttga
     3241 tttgcgtacc ctgaaagaga cgcgtattga acacgtcggc gctggaggct gtaccgtctt
     3301 gcgtaaaaac tcgcgctact tgttcgctgg agatcaattg ggcaccgtta ccctgcgtga
     3361 tttgaattcc ttgtccgtgc aacataccat taaaacccat acgaatattc tgagcgattt
     3421 ttccgtgcaa ggcaacttgc tgattagttg tggctacagc ggaaggcaaa ataacctggc
     3481 catcgaccgc ttcctaatgg tctacgatct gcgcatgctg cgcctgatcg cgcccattca
     3541 agtgatgatt gatccgcaga tgttgaagtt cctgccatcg ctgacatccc aactggccgt
     3601 cgtgtccagc tacggccagg ttcagctggt ggacactgtg gagctcagcg agccgcgagt
     3661 atccatgtac cagatcaaca ccaacggcag ccagtgcctc agcttcgaca ttagctcctc
     3721 ttcacaggcc atggcatttg gggaccagtc gggccacatc aacatgattg ccgctgtaca
     3781 aacgcctcag ccgcagttca atctctactc gcgaagcaca gagtttgcgg acgtggtgcc
     3841 acagctgccg atggtctcca ttacggacac caactttccg ctatcgtcgg tcatgctacc
     3901 gcacttgacg acgggcacac aatggttctc agattggccg gaggaactgc tgcgctaccg
     3961 ctaccacagg cccaagacca ttgatcccga agtgctgagc aacatgaaaa tgcagggacc
     4021 gattggatac tcgccaaacc cacgcaccgc ccgtcgtaat caaattccgt acgtgattga
     4081 gcagggcggc gtgtgcagtc cgaacggaaa tggaactgcg gcggcgacca aggcggagaa
     4141 cggtgtgaaa atcattccac ggcggtatcg caaagtcgag ctgaagtaca cgaagctggg
     4201 cacccaggac tttgactttg accagcataa tcaaacatgt ttcgcggggc tggaggcaac
     4261 gctaccgaac tcctactgca atgctatgct acagattttg tatttcaccg acgcgcttcg
     4321 cgtgaagctg ctggagcatt cgtgcatcaa ggagttttgc ctgtcctgcg aactagggtt
     4381 cctcttcaac atgctggata aaagtaccgc ttcttcacct tgccaggcca gcaatttcct
     4441 gcgatctttc cgcactgtgc cagaggcctc ggcactggga ttgattctca ccgaccgctc
     4501 atcgaacgtc aatctaatat cgctgataca gaactggaac cgattcattt tgcatcaaat
     4561 gcattacgaa atattcgatt ctagcaagaa cgcatccacc tacagcggat ctgtgcaaac
     4621 atcaacgaac gcggaaaacg cgggctcttc agaaacaagt ggctcctcgg acctttacga
     4681 ttccataagt gatgagaact ccaaggagga cgatcgggaa cgcagtaaga tcaacgcaga
     4741 aactgacatc agtaagatct ttgggaccaa gcaaatctgt ataaaccgct gcatcaaatg
     4801 ccaagaagag aagataaagg aaagcattct acttgcctgc aatttgtcgt atcccaacca
     4861 catcaaagat agtgatcagt atttcaactt tggcaccatt ttgaagcgat ctctcagctc
     4921 cgagaagagt attcaggcct tctgcgagag atgtaagaag ttttcgccaa ccaatcagag
     4981 cgtgaaggtg acttcgcttc cgcaaattct gtccattaac tgtggcctca acaacgaaaa
     5041 agacattacc ttcctgaagc gccagctcaa tcgctgcagc gaaaagacga ctgttgatgc
     5101 ggccgcttcc ttgagcacaa gcaagccgtg tcgatatgga gccaactgtt cgcgaagtga
     5161 ctgccatttc atgcatccgg atcgaaaatc tccatcgcac acaagccaac cgaatgctgt
     5221 caacaattct cccaatggac gccaaaagtc ctggtttccc cttacattta ctatgggcat
     5281 caatgaccaa ggtgaggtgc aggtgcaaac tcaatcagat gcaagcagtg gcaaatcgga
     5341 acaggaggag gaaacggaaa agccaccaac aaagggcttg gacaacaatc ggatgtacgc
     5401 gctgcatgcc gtggtgtgcc aagtcgatga tggtacccaa aagaacttgg tatcgttgat
     5461 aaatgtgcag cggccatacc acaccatgaa actggctgaa tctgcagatg accctcagag
     5521 tcaatggtat atttttaatg attttagcat atcgcccgtt tcgccacaag aatcggtatg
     5581 gttcacattg gattggaaag ttccttgcat actcttctac agacacgtag aggatgactc
     5641 tgagtctgct agtggcagca gcggctcggg tgatctggtc gcaatggatg ccgagttcgt
     5701 taccctaaat ccagaggaaa atgagattcg cccggatggc aaaacggcga ccattaagcc
     5761 atgccacatg agtgtggccc ggatttcatg tatacgagga caaggcccgg ctgaaggagt
     5821 tccattcatg gacgattaca tatccaccca ggagaaggtc gtggactacc taactcagtt
     5881 ctcgggaatc aagcctggcg acttggacgc taacttcagc aaaaagcgac tgactgcctt
     5941 gaagtactca taccagaaat taaaatacct cgttgacgta ggagtaatat ttgtcggaca
     6001 tggtctgaag aacgacttta gggtcataaa catctatgta ccgtcggagc agataataga
     6061 tacggtgcac ctgtttcaca tgccacatca tcgcatggtt tccctaagat ttctagcctg
     6121 gcatttcttg ggtactaaga ttcagtccga gacgcacgac tccatcgaag atgcacggac
     6181 cactctgcag ctctacaagc actatttgaa gctgcaggag gagaagaagt ttgccaacgc
     6241 tctcaagaat ctttacgaac gcggaaagca gctgcagtgg aaggttccag aggactgacc
     6301 gcggttcgaa ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgcgtaccgg
     6361 tcatcatcac catcaccatt gagtttaaac ccgctgatca gcctcgactg tgccttctaa
     6421 ggcctgagct cgctgatcag cctcgatcga ggatccagac atgataagat acattgatga
     6481 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
     6541 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
     6601 cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa
     6661 cctctacaaa tgtggtatgg ctgattatga tcagtcgacc gatgcccttg agagccttca
     6721 acccagtcag ctccttccgg tgggcgcggg gcatgactat cgtcgccgca cttatgactg
     6781 tcttctttat catgcaactc gtaggacagg tgccggcagc gctctgggtc attttcggcg
     6841 aggaccgctt tcgctggagc gcgacgatga tcggcctgtc gcttgcggta ttcggaatct
     6901 tgcacgccct cgctcaagcc ttcgtcactg gtcccgccac caaacgtttc ggcgagaagc
     6961 aggccattat cgccggcatg gcggccgacg cgctgggcta cgtcttgctg gcgttcgcga
     7021 cgcgaggctg gatggccttc cccattatga ttcttctcgc ttccggcggc atcgggatgc
     7081 ccgcgttgca ggccatgctg tccaggcagg tagatgacga ccatcaggga cagcttcaag
     7141 gatcgctcgc ggctcttacc agccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     7201 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     7261 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     7321 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     7381 cgggaagcgt ggcgctttct caatgctcac gctgtaggta tctcagttcg gtgtaggtcg
     7441 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     7501 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     7561 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     7621 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
     7681 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     7741 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     7801 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     7861 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     7921 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     7981 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     8041 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     8101 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     8161 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     8221 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     8281 ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     8341 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     8401 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     8461 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     8521 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     8581 caacacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     8641 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     8701 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     8761 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     8821 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     8881 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     8941 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
     9001 ataggcgtat cacgaggccc tttcgt
//
