LOCUS       sequence-29071-e         660 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Chen H, Detmer SA, Ewald AJ, Griffin EE, Fraser SE, Chan DC
  TITLE     Mitofusins Mfn1 and Mfn2 coordinately regulate mitochondrial fusion 
            and are essential for embryonic development.
  JOURNAL   J Cell Biol. 2003 Jan 20. 160(2):189-200.
  PUBMED    12527753
REFERENCE   2  (bases 1 to 660)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2003 Jan 20. 160(2):189-200."
COMMENT     Sequence Label: Cox4-DsRed retrovirus
FEATURES             Location/Qualifiers
     source          1..660
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      149..158
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(168..233)
                     /codon_start=1
                     /product="mitochondrial presequence of yeast cytochrome c 
                     oxidase subunit IV (Hurt et al., 1984)"
                     /label=pre-CoxIV
                     /translation="MLSLRQSIRFFKPATRTLCSSR"
     primer_bind     complement(299..318)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(380..402)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
ORIGIN
        1 cttggtccct tcagcttggc ggtctgggtg ccctcgtagg ggcggccctc gccctcgccc
       61 tcgatctcga actcgtggcc gttcacggag ccctccatgc gcaccttgaa gcgcatgaac
      121 tccttgatga cgttcttgga ggagcgcacc atggtggcga ccggtggtct agagctacac
      181 aaagttcttg tggctggctt gaaaaatctt atagattgac gtagtgaaag catggtgaat
      241 ttcgagggat cccgggcccg cggtatcaaa tcaattcttt gccaaaatga tgagacagca
      301 caataaccag cacgttgccc aggagctgta ggaaaaagaa gaaggcatga acatggttag
      361 cagaggctct agagccgccg gtcacacgcc agaagccgaa ccccgccctg ccccgtcccc
      421 cccgaaggca gccgtccccc cgcggacagc cccgaggctg gagagggaga aggggacggc
      481 ggcgcggcga cgcacgaagg ccctccccgc ccatttcctt nctgccggcg ccgcaccgct
      541 tcgccccgcg cccgctagag ggggtgcggc ggcgcctccc agatttcggc tccgcacaga
      601 tttgggacaa aggaagtccc tgcgccctct cgcacgatta ccataaaagg caatggctgc
//
