LOCUS       sequence-29111-e         927 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Lee HH, Kim HS, Kang JY, Lee BI, Ha JY, Yoon HJ, Lim SO, Jung G, Suh
            SW
  TITLE     Crystal structure of human nucleophosmin-core reveals plasticity of 
            the pentamer-pentamer interface.
  JOURNAL   Proteins. 2007 Nov 15. 69(3):672-8.
  PUBMED    17879352
REFERENCE   2  (bases 1 to 927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proteins. 
            2007 Nov 15. 69(3):672-8."
COMMENT     Sequence Label: B23(9-122)
FEATURES             Location/Qualifiers
     source          1..927
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             374..391
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(444..462)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      458..505
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      542..920
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(629..648)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ttaactttaa gaaggagata tacatatgag ccccctgagg ccccagaact atcttttcgg
       61 ttgtgaacta aaggccgaca aagattatca ctttaaggtg gataatgatg aaaatgagca
      121 ccagttatct ttaagaacgg tcagtttagg ggctggtgca aaggatgagt tgcacattgt
      181 tgaagcagag gcaatgaatt acgaaggcag tccaattaaa gtaacactgg caactttgaa
      241 aatgtctgta cagccaacgg tttcccttgg gggctttgaa ataacaccac cagtggtctt
      301 aaggttgaag tgtggttcag ggccagtgca tattagtgga cagcacttag tagctgtgga
      361 ggaagatctc gagcaccacc accaccacca ctgagatccg gctgctaaca aagcccgaaa
      421 ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc
      481 taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat tggcgaatgg
      541 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
      601 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
      661 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
      721 agtgctttac ggcacctcga ccccaaaaaa cttgantagg gtgatggttc acgtagtggg
      781 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
      841 ggactcttgt tccanctgga acaacactca accctatctc ggnctattct ttgatttata
      901 agggattttg ccgatttcgg cctattg
//
