LOCUS       sequence-291375-        9997 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmGIGYF1-del331-370-YFWFtoAAAA-dsRNAres.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9997)
  AUTHORS   Ruscica V, Bawankar P, Peter D, Helms S, Igreja C, Izaurralde E
  TITLE     Direct role for the Drosophila GIGYF protein in 4EHP-mediated mRNA 
            repression.
  JOURNAL   Nucleic Acids Res. 2019 Jul 26;47(13):7035-7048. doi: 
            10.1093/nar/gkz429.
  PUBMED    31114929
REFERENCE   2  (bases 1 to 9997)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2019-07-26- 26"; volume: "47"; issue: 
            "13"; pages: "7035-7048"
COMMENT     Sequence Label: pAc5.1B-HA-DmGIGYF-del331-370-YFWFtoAAAA-dsRNA...
FEATURES             Location/Qualifiers
     source          1..9997
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2568
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2594..2620
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             7282..7323
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             7333..7350
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7373..7390)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7423..7557
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7447..7466)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7501..7520
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(8179..8767)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8259..8278)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8938..9729,9730..9798))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9561..9580
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9799..9903)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9971..9989
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
      241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
      301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
      361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
      421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
      481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
      541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
      601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
      661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
      721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
      781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
      841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
      901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
      961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
     1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
     1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
     1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
     1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
     1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
     1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
     1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
     1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
     1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
     1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
     1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
     1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
     1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
     1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
     2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
     2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
     2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
     2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
     2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
     2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
     2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
     2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
     2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
     2581 taccaacatg gcttacccat acgatgttcc agattacgct cctcctccaa gcttgaattc
     2641 tatgactgac tccatgaagt tcggacctga gtggctgcga aacatgagcg cagaaccctc
     2701 cggtagccca tccacgtata atgtgggaaa cgcggcccag aataacagca taggcggaca
     2761 taatctcggc aataatactg cagcgtcggc atcgcgaaac ctcttccccg agtatcgcta
     2821 tggtcgggaa gagatgctca gtctctttga ccgtaattgt ctgctcccac aaattttgcc
     2881 ttcctttaag aaactgtttg tcgagaaagt ccaatgtccg ctggcgctca cccctagtag
     2941 cgaagaagat attaatcaga attccctcgg aaacaatagc cgcccagcat ggctccaaag
     3001 gtccccgtcg ggtttcggat ccgctagtcg cggaagcggt cgtggcggta cagtggatcg
     3061 tggtcgcatg aggggcaagt ccgcgtacca cccaatttat cagcgtcctt cgggcctgta
     3121 cgacgagagt ctctccgtga tcagtaaagc tgagcggact tggtccgatc gtaatggcac
     3181 aggcgacagt gctgctacaa ctactagtac gtcgggtagt ggagcgttgg actggaacgg
     3241 tacgcctagt agctcgccac ggaaggacta ctccaacaac caccgtaatc tggagaattg
     3301 gcgtcggact cgaaatgagg acggcagtgg cgacggcccg tcgagttccg gctcgatgag
     3361 tggccctgat attgctggtt ggcggagcgg tgtcgtcggt ggaagtacaa acgcaggttt
     3421 tggtaccaat agccatcggt ggggaagatc tacaagctgg cgtgatgaag atcctagcgt
     3481 cgacaatgca gctagtttac aacgctcaat ttcaacagtt gcgactttaa gtacagatcg
     3541 gactggaaac aataaaggat ctggaatcgg agctgcagag ggagtaggga gcatatcgca
     3601 cccacggttg tcgagttcta aaatatctca aactgatcta aaacctatca aaagttcgca
     3661 taatacttta aaaaccaaaa gcttagactc ctatgacgat gtaaaaagac cgaaaagtaa
     3721 agacctgtct gatccagata gtggaaacga tataactcca gaaacctcat taacgaaaga
     3781 taccaataca acagctgtgc aagaagaagt tgaaagcagt ttatctccaa taagctctac
     3841 aacgacaaag gaagtgatcc atggagatat ttcagatcgg attaaagagg tcgccgatga
     3901 agtagaaaaa cttataatgg atgatgatca taaaatctct tcgaatcaaa gccaacatca
     3961 aaatcatgac gggtttacag ctgcacttcc aagaatggca gacattgaga taaacgtaaa
     4021 accgagtgtt acagcagtgc atcggcaagc accttccacc atgcctatac aaattacccc
     4081 tacaattaca gatgtcgctc caccatccca tgcagttgtg tcgttttcgg atcatgagac
     4141 aatgcaacac cataacatgc accacctgcc acaatttcca atgattccta caccgcatat
     4201 catcactcca aatctaaacg aattgtggtt tgcccgggat ccgcaggcaa atgttcaggg
     4261 gccagctagt gccgttgaga tgacggaagc gtatcgcgct ggctacgcta atgagaacct
     4321 gtttgtacgc cggtactctg ataataggtt tagaccgctg ggagagctta taaaattttg
     4381 tcatggtaac atgccattta cgcacagtca cctacttcca tctccgatag aactagagaa
     4441 cattcctgtt ggtcaaatac cagcccctct aacggcgtca ctttcaatta caccccataa
     4501 gccatcaccg attcctatcg cactttctgt tgttgaacag cagttgcagc aacaaagaga
     4561 tgagcaacta aaggcaaatg ttacagcaac agctgaagct ctacgtgctg caataaaagg
     4621 aagttttggc ggaaatagca ttggtaatac gtctcatttg cttacaatgc ggtttcaaat
     4681 gctacaggat cagtacatac agcaccagga ataccaaata ctagctgagc tatccaaaaa
     4741 tgaatgcttt caacgtcttt cggctgtcga gcaagaaaca gttgttcgtc ggaaagttca
     4801 attgctgggt cttcctgagt atttgatcag cttaaacgga ttaagcaatt ctttgtccgt
     4861 actgaacccc gttgccggta gacagttgta cagggcagtg gttgagcacg ccaagaagga
     4921 tcaacaacat atttttgcaa acaccgagca acaacgttca gtgggaaatt tactggatgc
     4981 taataatttc attctaaatg cccaaataat gattcagcaa tcgcagcaag aggtaggtcc
     5041 cttggtatca tccgtggatt gtattatgca aggtggaact gcagctgacc taaataagcc
     5101 gaatgaaata ccgaggaatg agttagactt aattaatgaa tataacttac ggatgctgtt
     5161 gcgtggccaa ccaacaagta ctcaacagca accacctgcg ctgacgaact cagctaccga
     5221 aaatctccct ggagttgatt ttttaactga aacacaattg ttagagcggc aaaacttgat
     5281 gattccaatc tggttacccc ctaataataa taagcaacaa gaaaccgacc aacagtgggc
     5341 tgaaatgtct aatgcggatg catcattatg ggaaacggcc aacttaaatg aagagcgaaa
     5401 tgaagatcag caactattaa tacaaaagtc ttctgaagcg tgcttcgcag atacagaaaa
     5461 agatgtcaaa attgcccaat tatttcaagt tcaatcagga aatgttgtta accacaccgc
     5521 attagaggaa ttagatcagt cccctcaaaa tttaaaaggt tcacataatc aaaaaatagt
     5581 caaatccctt gtttccgata ttcaacagaa tcataatgag gaactaaatt cacatcaaca
     5641 ccaggtaaag caggctaaca agcagaatct gaatacaaaa cagaatgcgg cacagtcagc
     5701 cctaaagcca attaataacg aaaatgatcg caaacgagaa cagacagaag aaaaaaaaag
     5761 gcaaagagag gaacgcaagc gccagcaatt ggaagatgat aaacgtcggg cgttgaatga
     5821 atctgaagaa cagacccgcc aaattcaaga ggaaaaggaa aggcaacagc aaatacaagc
     5881 ccaacgtcga aaggcattgt tgggcaatgt ccattctcta tcagttcaaa atgggatgtc
     5941 aggaacatta gcgtctgcac aaagcaagaa gaacgacgat gccaaaacag cagaacccca
     6001 agtatcctcg cgcttgccgt ctacatccgt agcgccttgg tcttttcagt tgcaaaattc
     6061 tatgagatcg gcgcctggtc tggcagaaat acaaaaggca gaacgtcgag agcgtcgcgc
     6121 agaccaacag cgacatcaag agctattaga caagcaattg cgtgccaatg ccgcagctgc
     6181 ggctgaagcc aacgatgctt tgcttaaatg gcagtcaaca ccagcgtcgg cgcccgtaat
     6241 gagtctcgcc gaaattcaag cagaagaggc aaggcggttg gccaatgacc ttgtggatcg
     6301 gcagcgtcga cgagaactgg aacatcacca acaagctcct ttgtcatcag cggttttggt
     6361 tacgagtgca acttccaaca tctggggtaa cgccaataaa gcatggagtt cgtccgctgc
     6421 tcaatcactt tcgttaaaaa caagttctgg aactggtctg tgggacgaac cgaatccatt
     6481 aggttctaat ggatctggaa caagcggtac ctcatccgta actgcggcgg cagtcttggc
     6541 aggaggatta aactcagcta ataaatccac tctgcaagct caaaataagt cgtcggcttt
     6601 atttgcatcg cctcgaaatt tgcgcaagag tcaaacattg ccagccctta gtaacccaga
     6661 aaagtcaaat aaaaatggac caggtcaacg gccagaaaaa caaaagttgg ctcaaactcg
     6721 ttcaaagggt gccgctgtct caattgaaga gaaggataga gaaagaaaat tgaatgcaaa
     6781 aagccagcaa agcagcaccg accaagccat tagcaaggtt aatgagtatg aaaacgagtt
     6841 cactagctgg tgcataaaga gcctagataa tatgtccgcc aaagttgatg tacccacgtt
     6901 cgttgcattc ttgcaggact tggaagcgcc atatgaggta aaggactatg tccgaatata
     6961 ccttggcgat ggaaaagatt ctttggattt cgcaaagcag tttttggagc gacgtagcaa
     7021 atacaaaagc ttgcaacgtg cccaaaaggc acacaatgac gatatgtgca aaccagctcc
     7081 tgccattacg ccatctgcga atgactatgc cgacagcaag aacaaacaaa aaaagattaa
     7141 aaagaataag atgactaaga tggacgcccg cattcttggg ttttcagtaa ctgctgccga
     7201 gggtcgcata aatgttggca ttcgagacta cgtcgaagga ccataagcgg ccgctcgagt
     7261 ctagagggcc cgcggttcga aggtaagcct atccctaacc ctctcctcgg tctcgattct
     7321 acgcgtaccg gtcatcatca ccatcaccat tgagtttaaa cccgctgatc agcctcgact
     7381 gtgccttcta aggcctgagc tcgctgatca gcctcgatcg aggatccaga catgataaga
     7441 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt
     7501 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac
     7561 aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa
     7621 agcaagtaaa acctctacaa atgtggtatg gctgattatg atcagtcgac cgatgccctt
     7681 gagagccttc aacccagtca gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc
     7741 acttatgact gtcttcttta tcatgcaact cgtaggacag gtgccggcag cgctctgggt
     7801 cattttcggc gaggaccgct ttcgctggag cgcgacgatg atcggcctgt cgcttgcggt
     7861 attcggaatc ttgcacgccc tcgctcaagc cttcgtcact ggtcccgcca ccaaacgttt
     7921 cggcgagaag caggccatta tcgccggcat ggcggccgac gcgctgggct acgtcttgct
     7981 ggcgttcgcg acgcgaggct ggatggcctt ccccattatg attcttctcg cttccggcgg
     8041 catcgggatg cccgcgttgc aggccatgct gtccaggcag gtagatgacg accatcaggg
     8101 acagcttcaa ggatcgctcg cggctcttac cagccagcaa aaggccagga accgtaaaaa
     8161 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     8221 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     8281 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     8341 ctttctccct tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt atctcagttc
     8401 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     8461 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     8521 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     8581 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     8641 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     8701 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     8761 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     8821 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     8881 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     8941 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     9001 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     9061 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     9121 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     9181 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     9241 tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     9301 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     9361 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     9421 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     9481 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     9541 ttgcccggcg tcaacacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     9601 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     9661 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     9721 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     9781 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     9841 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     9901 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
     9961 aacctataaa aataggcgta tcacgaggcc ctttcgt
//
