LOCUS       sequence-29163-e         748 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Ibarra JA, Knodler LA, Sturdevant DE, Virtaneva K, Carmody AB, 
            Fischer ER, Porcella SF, Steele-Mortimer O
  TITLE     Induction of Salmonella pathogenicity island 1 under different 
            growth conditions can affect Salmonella-host cell interactions in 
            vitro.
  JOURNAL   Microbiology (Reading). 2010 Apr;156(Pt 4):1120-1133. doi: 
            10.1099/mic.0.032896-0. Epub 2009 Dec 24.
  PUBMED    20035008
REFERENCE   2  (bases 1 to 748)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Microbiology (Reading)."; date: "2010-04"; volume: "156(Pt 
            4):1120-1133. doi"; pages: " 10.1099/mic.0.032896-0. Epub 2009 Dec 
            24"
COMMENT     Sequence Label: pMPMA3dPlac-PprgH-GFPLVA
FEATURES             Location/Qualifiers
     source          1..748
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     46..62
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     46..62
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     RBS             494..505
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     primer_bind     complement(542..570)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 cgactcctat agggcgaatt ggagctccac cgcggtggcg gccgctctag aactagtgga
       61 tcccccgggc tgcaggaatt cgatatcaag cttgcatgcc tgcaggtcga gttgtaaatg
      121 ctctttatta gcgtgtgttg gcaatggtct gattgttaca ccaaaagaac ccaaatttgg
      181 gtaatttatc tacagtagtt taagccccaa tggggatgat ggttctttta atatgtgttg
      241 agacgcatta tacagaataa attgatttta tttctcactt ttcattctat tttcatcagg
      301 aatccctgtg tcctgtgcgg taatctgctg ctatcgagaa cgacagacat cgctaacagt
      361 atatatggaa acatcaaaag agaagacgat aacaagccca gggccataca tagttcgatt
      421 acttaacagc tcactgaacg gctgtgagtt tccattgctg acaggccgaa cactctttgt
      481 ggtacctaga attaaagagg agaaattaag catgcgtaaa ggagaagaac ttttcactgg
      541 agttgtccca attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtcag
      601 tggagagggt gaaggtgatg caacatacgg aaaacttacc cttaaattta tttgcactac
      661 tggaaaacta cctgttccat ggccaacact tgtccaanct tcgtttgtgg ggtcnagggc
      721 tttgcgagat acccgatcat atgaanac
//
