LOCUS       sequence-29184-e         709 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Johannessen CM, Boehm JS, Kim SY, Thomas SR, Wardwell L, Johnson LA,
            Emery CM, Stransky N, Cogdill AP, Barretina J, Caponigro G, 
            Hieronymus H, Murray RR, Salehi-Ashtiani K, Hill DE, Vidal M, Zhao 
            JJ, Yang X, Alkan O, Kim S, Harris JL, Wilson CJ, Myer VE, Finan PM,
            Root DE, Roberts TM, Golub T, Flaherty KT, Dummer R, Weber BL, 
            Sellers WR, Schlegel R, Wargo JA, Hahn WC, Garraway LA
  TITLE     COT drives resistance to RAF inhibition through MAP kinase pathway 
            reactivation.
  JOURNAL   Nature. 2010 Dec 16;468(7326):968-72. doi: 10.1038/nature09627
  PUBMED    21107320
REFERENCE   2  (bases 1 to 709)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2010-12-16- 16"; volume: "468"; issue: "7326"; 
            pages: "968-72"
COMMENT     Sequence Label: pDONR223-BMP2K
FEATURES             Location/Qualifiers
     source          1..709
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    10..109
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(615..638)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 cagctggagg caataatgat tttattttga ctgatagtga cctgttcgtt gcaacaaatt
       61 gataagcaat gctttcttat aatgccaact ttgtacaaga aagttggcaa ttgtgaagca
      121 aaataagcct tcaagtgtcc cttacttgat cttagaaggt caaattctct gtccaatagt
      181 tcctcttctg ttaacttaga gaaattatcc tctccaaaag gattccaccc tgacatatca
      241 ggtggattgc tgatgttctt ctgatttgtg aaatttgcaa tggcctcttt atcagcaact
      301 gacctattgg cactatagga ggagtccatt atggttccaa cctgagctgg aagtgatgaa
      361 gtgtaggaaa ctaaggctgg tgagaactct tgaggggagg taagatattc aggtgatgcc
      421 tgttgttgag acggctgatg ttgagctagc atctgctgtt gaaagaaagc ctgctgatac
      481 tgcggcatca ttgtaggata ctgtgatgca gaaggttgtg gttgatatac cgaatgcatt
      541 aaaaattgtt gttgaagcat ctgttgctgc tgtgttgcat gttgatactg ctgcatataa
      601 gcatcttgaa gtaggtggtg gtggtggtgg tggtggtgct gctgctgctg ctgctgctgc
      661 tgctgttgct gttgctgctg ctgctgttgc tgttgctgct gctgctgct
//
