LOCUS       sequence-291986-       14119 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression vector for an inducible BirA-CDKN1A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14119)
  AUTHORS   Pereira EJ, Burns JS, Lee CY, Marohl T, Calderon D, Wang L, Atkins 
            KA, Wang CC, Janes KA
  TITLE     Sporadic activation of an oxidative stress-dependent NRF2-p53 
            signaling network in breast epithelial spheroids and 
            premalignancies.
  JOURNAL   Sci Signal. 2020 Apr 14;13(627). pii: 13/627/eaba4200. doi: 
            10.1126/scisignal.aba4200.
  PUBMED    32291314
REFERENCE   2  (bases 1 to 14119)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2020-04-14"; volume: "13(627). pii: 
            13/627/eaba4200. doi"; pages: " 10.1126/scisignal.aba4200"
COMMENT     Sequence Label: pSLIK_hygro_BirA_CDKN1A
FEATURES             Location/Qualifiers
     source          1..14119
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..580
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             598..778
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    825..950
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1443..1676
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1861..1905
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2203..2320
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2377..2401
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        2433..2747
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    2441..2459
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2477..2495
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2512..2530
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2548..2566
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2584..2602
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2619..2637
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2655..2673
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2720..2744
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(2754..3104,3105..3107,3108..3716)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     CDS             3723..4217
                     /codon_start=1
                     /gene="Homo sapiens CDKN1A"
                     /product="human cyclin-dependent kinase inhibitor 1A"
                     /label=p21
                     /note="mediates p53-dependent arrest in the G1 phase of the
                     cell cycle"
                     /translation="MSEPAGDVRQNPCGSKACRRLFGPVDSEQLRRDCDALMAGCIQEA
                     RERWNFDFVTETPLEGDFAWERVRGLGLPKLYLPTGPRRGRDELGGGRRPGTSPALLQG
                     TAEEDHVDLSLSCTLVPRSGEQAEGSPGGPGDSQGRKRRQTSMTDFYHSKRRLIFSKRK
                     P"
     protein_bind    complement(4253..4277)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        4327..5538
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5375..5394
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(5663..6280,6281..6286,6287..6322,6323..6325,6326..6361,
                     6362..6364,6365..6400,6401..6409)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    6564..7026
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6620..6637)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6847..6866
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7085..8104
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     CDS             7085..8104
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     misc_feature    8570..9158
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8623..8643)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9041..9052)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9161..9177)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9162..9178)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             9687..9867
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9893..9910)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9899..10123
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      10169..10597
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10256..10275)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10466..10487
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(10606..10626)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10611..10940
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10791..10926
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10853..10872
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        10988..11035
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             11054..11428
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    11558..11679
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11595..11614)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11649..11668
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(11728..11744)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11728..11744)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11741..11763)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11752..11768
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11776..11782,11783..11800,11801..11806))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11821..11842
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11959..11976)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12130..12718)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12210..12229)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12889..13680,13681..13749))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13512..13531
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13750..13854)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(13929..13948)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
      601 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
      661 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
      721 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
      781 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
      841 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
      901 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
      961 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1021 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1081 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1141 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     1201 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     1261 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     1321 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     1381 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     1441 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     1501 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     1561 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     1621 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     1681 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     1741 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     1801 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     1861 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     1921 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     1981 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2041 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2101 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
     2161 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     2221 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     2281 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     2341 cagagatcca gtttggttaa ttctagacag ttatcaacaa gtttgtacaa aaaagcaggc
     2401 tttaaaggaa ccaattcagt cgactggatc ccgagtttac tccctatcag tgatagagaa
     2461 cgtatgtcga gtttactccc tatcagtgat agagaacgat gtcgagttta ctccctatca
     2521 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgagtt
     2581 tactccctat cagtgataga gaacgtatgt cgagtttatc cctatcagtg atagagaacg
     2641 tatgtcgagt ttactcccta tcagtgatag agaacgtatg tcgaggtagg cgtgtacggt
     2701 gggaggccta tataagcaga gctcgtttag tgaaccgtca gatcgccact agaatgaagg
     2761 acaacaccgt gcccctgaag ctgatcgccc tgctggccaa cggcgagttc cactctggcg
     2821 agcagctggg agagaccctg ggaatgagca gagccgccat caacaagcac atccagacac
     2881 tgagagactg gggagtggac gtgttcaccg tgcctggcaa gggctacagc ctgcctgagc
     2941 ctatccagct gctgaacgcc aagcagatcc tgggacagct ggatggcgga agcgtggccg
     3001 tgctgcctgt gatcgactcc accaatcagt acctgctgga cagaatcgga gagctgaagt
     3061 ccggcgacgc ctgcatcgcc gagtaccagc aggctggcag aggaggcaga ggacggaagt
     3121 ggttcagccc attcggagcc aacctgtacc tgtccatgtt ctggagactg gagcagggac
     3181 ctgctgctgc catcggactg agtctggtga tcggaatcgt gatggccgag gtgctgagaa
     3241 agctgggagc cgacaaggtg agagtgaagt ggcctaatga cctgtacctc caggaccgca
     3301 agctggctgg catcctggtg gagctgacag gcaagacagg cgatgccgct cagatcgtga
     3361 tcggagccgg aatcaacatg gccatgagaa gagtggagga gagcgtggtg aaccagggct
     3421 ggatcaccct gcaggaggct ggcatcaacc tggaccggaa caccctggcc gccatgctga
     3481 tcagagagct gagagccgct ctggagctgt tcgagcagga gggactggct ccttacctga
     3541 gcagatggga gaagctggac aacttcatca acagacctgt gaagctgatc atcggcgaca
     3601 aggaaatctt cggcatctcc agaggaatcg acaagcaggg agctctgctg ctggagcagg
     3661 acggaatcat caagccctgg atgggcggag aaatctccct gagaagcgca gagaagacta
     3721 gtatgtcaga accggctggg gatgtccgtc agaacccatg cggcagcaag gcctgccgcc
     3781 gcctcttcgg cccagtggac agcgagcagc tgagacgcga ctgtgatgcg ctaatggcgg
     3841 gctgcatcca ggaggcccgt gagcgatgga acttcgactt tgtcaccgag acaccactgg
     3901 agggtgactt cgcctgggag cgtgtgcggg gccttggcct gcccaagctc taccttccca
     3961 cggggccccg gcgaggccgg gatgagttgg gaggaggcag gcggcctggc acctcacctg
     4021 ctctgctgca ggggacagca gaggaagacc atgtggacct gtcactgtct tgtacccttg
     4081 tgcctcgctc aggggagcag gctgaagggt ccccaggtgg acctggagac tctcagggtc
     4141 gaaaacggcg gcagaccagc atgacagatt tctaccactc caaacgccgg ctgatcttct
     4201 ccaagaggaa gccctaacaa ttggaagact aatgcgtttt cgagatatct agacccagct
     4261 ttcttgtaca aagtggttga taacatctcg agccataaag atggttaatt aacccaccca
     4321 agatctggcc tccgcgccgg gttttggcgc ctcccgcggg cgcccccctc ctcacggcga
     4381 gcgctgccac gtcagacgaa gggcgcagcg agcgtcctga tccttccgcc cggacgctca
     4441 ggacagcggc ccgctgctca taagactcgg ccttagaacc ccagtatcag cagaaggaca
     4501 ttttaggacg ggacttgggt gactctaggg cactggtttt ctttccagag agcggaacag
     4561 gcgaggaaaa gtagtccctt ctcggcgatt ctgcggaggg atctccgtgg ggcggtgaac
     4621 gccgatgatt atataaggac gcgccgggtg tggcacagct agttccgtcg cagccgggat
     4681 ttgggtcgcg gttcttgttt gtggatcgct gtgatcgtca cttggtgagt agcgggctgc
     4741 tgggctggcc ggggctttcg tggccgccgg gccgctcggt gggacggaag cgtgtggaga
     4801 gaccgccaag ggctgtagtc tgggtccgcg agcaaggttg ccctgaactg ggggttgggg
     4861 ggagcgcagc aaaatggcgg ctgttcccga gtcttgaatg gaagacgctt gtgaggcggg
     4921 ctgtgaggtc gttgaaacaa ggtggggggc atggtgggcg gcaagaaccc aaggtcttga
     4981 ggccttcgct aatgcgggaa agctcttatt cgggtgagat gggctggggc accatctggg
     5041 gaccctgacg tgaagtttgt cactgactgg agaactcggt ttgtcgtctg ttgcgggggc
     5101 ggcagttatg gcggtgccgt tgggcagtgc acccgtacct ttgggagcgc gcgccctcgt
     5161 cgtgtcgtga cgtcacccgt tctgttggct tataatgcag ggtggggcca cctgccggta
     5221 ggtgtgcggt aggcttttct ccgtcgcagg acgcagggtt cgggcctagg gtaggctctc
     5281 ctgaatcgac aggcgccgga cctctggtga ggggagggat aagtgaggcg tcagtttctt
     5341 tggtcggttt tatgtaccta tcttcttaag tagctgaagc tccggttttg aactatgcgc
     5401 tcggggttgg cgagtgtgtt ttgtgaagtt ttttaggcac cttttgaaat gtaatcattt
     5461 gggtcaatat gtaattttca gtgttagact agtaaattgt ccgctaaatt ctggccgttt
     5521 ttggcttttt tgttagacga agcttggtac cgagctcgga tctccacccc gtaccggtcc
     5581 tgcaggattt aaatggcgcg ccgctagcgc taccggactc agatctcgag ctcaagcttc
     5641 gaattctgca gtcgaattca ccatgtctag actggacaag agcaaagtca taaacggagc
     5701 tctggaatta ctcaatggtg tcggtatcga aggcctgacg acaaggaaac tcgctcaaaa
     5761 gctgggagtt gagcagccta ccctgtactg gcacgtgaag aacaagcggg ccctgctcga
     5821 tgccctgcca atcgagatgc tggacaggca tcatacccac ttctgccccc tggaaggcga
     5881 gtcatggcaa gactttctgc ggaacaacgc caagtcatac cgctgtgctc tcctctcaca
     5941 tcgcgacggg gctaaagtgc atctcggcac ccgcccaaca gagaaacagt acgaaaccct
     6001 ggaaaatcag ctcgcgttcc tgtgtcagca aggcttctcc ctggagaacg cactgtacgc
     6061 tctgtccgcc gtgggccact ttacactggg ctgcgtattg gaggaacagg agcatcaagt
     6121 agcaaaagag gaaagagaga cacctaccac cgattctatg cccccacttc tgagacaagc
     6181 aattgagctg ttcgaccggc agggagccga acctgccttc cttttcggcc tggaactaat
     6241 catatgtggc ctggagaaac agctaaagtg cgaaagcggc gggccgaccg acgcccttga
     6301 cgattttgac ttagacatgc tcccagccga tgcccttgac gactttgacc ttgatatgct
     6361 gcctgctgac gctcttgacg attttgacct tgacatgctc cccgggtaac taagtaagga
     6421 tccgcggccg cactagagga attccgcccc tctccccccc ccmcycyccc ycctaacgtt
     6481 actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc
     6541 atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc
     6601 attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag
     6661 gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg
     6721 cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat
     6781 acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga
     6841 gtcaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc
     6901 cattgtatgg gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg
     6961 ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga
     7021 tgataagctt accggtacgc gtgatagctt gccacaaccc gtaccaaaga tggatagatc
     7081 cggaaagcct gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa agttcgacag
     7141 cgtctccgac ctgatgcagc tctcggaggg cgaagaatct cgtgctttca gcttcgatgt
     7201 aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct acaaagatcg
     7261 ttatgtttat cggcactttg catcggccgc gctcccgatt ccggaagtgc ttgacattgg
     7321 ggaattcagc gagagcctga cctattgcat ctcccgccgt gcacagggtg tcacgttgca
     7381 agacctgcct gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg ccatggatgc
     7441 gatcgctgcg gccgatctta gccagacgag cgggttcggc ccattcggac cgcaaggaat
     7501 cggtcaatac actacatggc gtgatttcat atgcgcgatt gctgatcccc atgtgtatca
     7561 ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc tcgatgagct
     7621 gatgctttgg gccgaggact gccccgaagt ccggcacctc gtgcacgcgg atttcggctc
     7681 caacaatgtc ctgacggaca atggccgcat aacagcggtc attgactgga gcgaggcgat
     7741 gttcggggat tcccaatacg aggtcgccaa catcttcttc tggaggccgt ggttggcttg
     7801 tatggagcag cagacgcgct acttcgagcg gaggcatccg gagcttgcag gatcgccgcg
     7861 gctccgggcg tatatgctcc gcattggtct tgaccaactc tatcagagct tggttgacgg
     7921 caatttcgat gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc gatccggagc
     7981 cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga ccgatggctg
     8041 tgtagaagta ctcgccgata gtggaaaccg acgccccagc actcgtccga gggcaaagga
     8101 atagagtaga tgccgaccga acaagagctg atttcgagaa cgcctcagcc agcaactcgc
     8161 gcgagcctag caaggcaaat gcgagagaac ggccttacgc ttggtggcac agttctcgtc
     8221 cacagttcgc taagctcgct cggctgggtc gcgggagggc cggtcgcagt gattcaggcc
     8281 cttctggatt gtgttggtcc ccagggcacg attgtcatgc ccacgcactc gggtgatctg
     8341 actgatcccg cagattggag atcgccgccc gtgcctgccg attgggtgca gatctctaga
     8401 tctgtacaag taaagcggcc gcgactctag atcataatca gccataccac atttgtagag
     8461 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
     8521 gcaattgttg ttgtttagtc cctcccaatt cgatatcaag cttatcgata atcaacctct
     8581 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
     8641 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     8701 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     8761 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     8821 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
     8881 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     8941 caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc
     9001 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     9061 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc
     9121 tcagacgagt cggatctccc tttgggccgc ctccccgcat cgataccgtc gacctcgatc
     9181 gagacctaga aaaacatgga gcaatcacaa gtagcaatac agcagctacc aatgctgatt
     9241 gtgcctggct agaagcacaa gaggaggagg aggtgggttt tccagtcaca cctcaggtac
     9301 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     9361 ggggactgga agggctaatt cactcccaac gaagacaaga tatccttgat ctgtggatct
     9421 accacacaca aggctacttc cctgattggc agaactacac accagggcca gggatcagat
     9481 atccactgac ctttggatgg tgctacaagc tagtaccagt tgagcaagag aaggtagaag
     9541 aagccaatga aggagagaac acccgcttgt tacaccctgt gagcctgcat gggatggatg
     9601 acccggagag agaagtatta gagtggaggt ttgacagccg cctagcattt catcacatgg
     9661 cccgagagct gcatccggac tgtactgggt ctctctggtt agaccagatc tgagcctggg
     9721 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     9781 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     9841 tttagtcagt gtggaaaatc tctagcaggg cccgtttaaa cccgctgatc agcctcgact
     9901 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
     9961 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
    10021 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
    10081 gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga
    10141 accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg
    10201 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
    10261 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
    10321 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
    10381 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
    10441 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
    10501 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
    10561 aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt
    10621 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
    10681 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
    10741 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
    10801 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
    10861 cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg
    10921 gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc
    10981 agcacgtgtt gacaattaat catcggcata gtatatcggc atagtataat acgacaaggt
    11041 gaggaactaa accatggcca agttgaccag tgccgttccg gtgctcaccg cgcgcgacgt
    11101 cgccggagcg gtcgagttct ggaccgaccg gctcgggttc tcccgggact tcgtggagga
    11161 cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg tccaggacca
    11221 ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg agctgtacgc
    11281 cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg ccatgaccga
    11341 gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc gacccggccg gcaactgcgt
    11401 gcacttcgtg gccgaggagc aggactgaca cgtgctacga gatttcgatt ccaccgccgc
    11461 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
    11521 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa
    11581 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
    11641 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac
    11701 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
    11761 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
    11821 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
    11881 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
    11941 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
    12001 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
    12061 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
    12121 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
    12181 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
    12241 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
    12301 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
    12361 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
    12421 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
    12481 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
    12541 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
    12601 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
    12661 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
    12721 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
    12781 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
    12841 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
    12901 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
    12961 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
    13021 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
    13081 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
    13141 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
    13201 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
    13261 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
    13321 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
    13381 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
    13441 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
    13501 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
    13561 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
    13621 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
    13681 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
    13741 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
    13801 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
    13861 tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc cctatggtgc
    13921 actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct ccctgcttgt
    13981 gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg
    14041 accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta
    14101 cgggccagat atacgcgtt
//
