LOCUS       sequence-292010-        8899 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral CRISPR guide vector expressing a eGFP-NT2 sgRNA with cs2 
            incorporated at the 3' end of the sgRNA constant region..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8899)
  AUTHORS   Replogle JM, Norman TM, Xu A, Hussmann JA, Chen J, Cogan JZ, Meer 
            EJ, Terry JM, Riordan DP, Srinivas N, Fiddes IT, Arthur JG, Alvarado
            LJ, Pfeiffer KA, Mikkelsen TS, Weissman JS, Adamson B
  TITLE     Combinatorial single-cell CRISPR screens by direct guide RNA capture
            and targeted sequencing.
  JOURNAL   Nat Biotechnol. 2020 Aug;38(8):954-961. doi: 
            10.1038/s41587-020-0470-y. Epub 2020 Mar 30.
  PUBMED    32231336
REFERENCE   2  (bases 1 to 8899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0470-y"; journalName: "Nat Biotechnol"; date: 
            "2020-08"; volume: "38"; issue: "8"; pages: "954-961"
COMMENT     Sequence Label: pBA900
FEATURES             Location/Qualifiers
     source          1..8899
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             210..390
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(452..1040)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(532..551)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1211..2002,2003..2071))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1834..1853
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2072..2176)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(2251..2270)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2442..2821
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2823..3021
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2976..2996
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             3039..3219
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3266..3391
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3888..4121
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4306..4350
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4648..4765
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    complement(5018..5051)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     5055..5075
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     promoter        5342..5553
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          5554..6492
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     6449..6469
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             6513..7109
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6513..6532)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7009..7029
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             7119..7172
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             7185..7883
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="SELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVV
                     EGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTAT
                     QDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKL
                     VGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCD
                     LPSKLGHKLN"
     protein_bind    complement(7903..7936)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    7992..8580
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8045..8065)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8463..8474)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8583..8599)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8584..8600)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
ORIGIN
        1 gatatccact gacctttgga tggtgctaca agctagtacc agttgagcaa gagaaggtag
       61 aagaagccaa tgaaggagag aacacccgct tgttacaccc tgtgagcctg catgggatgg
      121 atgacccgga gagagaagta ttagagtgga ggtttgacag ccgcctagca tttcatcaca
      181 tggcccgaga gctgcatccg gactgtactg ggtctctctg gttagaccag atctgagcct
      241 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
      301 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
      361 ccttttagtc agtgtggaaa atctctagca gcatgtgagc aaaaggccag caaaaggcca
      421 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
      481 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
      541 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
      601 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
      661 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
      721 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
      781 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
      841 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
      901 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
      961 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     1021 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     1081 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     1141 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     1201 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     1261 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     1321 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     1381 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     1441 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     1501 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     1561 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     1621 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     1681 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     1741 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     1801 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     1861 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     1921 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     1981 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     2041 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     2101 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     2161 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag
     2221 atctcccgat cccctatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     2281 ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta
     2341 agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg
     2401 ttttgcgctg cttcgcgatg tacgggccag atatacgcgt tgacattgat tattgactag
     2461 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
     2521 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
     2581 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
     2641 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
     2701 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
     2761 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
     2821 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
     2881 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
     2941 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
     3001 gtgggaggtc tatataagca gcgcgttttg cctgtactgg gtctctctgg ttagaccaga
     3061 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     3121 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     3181 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt
     3241 gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg
     3301 cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg
     3361 ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg
     3421 atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt
     3481 atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga
     3541 aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat cagaagaact
     3601 tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa
     3661 agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc
     3721 acagcaagcg gccggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg
     3781 agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc
     3841 aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc
     3901 cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta
     3961 caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt
     4021 gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga
     4081 atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct
     4141 ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg
     4201 gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca
     4261 agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa
     4321 ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg
     4381 tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt
     4441 gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc
     4501 cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag
     4561 agagacagag acagatccat tcgattagtg aacggatcgg cactgcgtgc gccaattctg
     4621 cagacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag
     4681 tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag aattacaaaa
     4741 acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg
     4801 gttagtaccg ggcccgctct agagatccga cgcgccatct ctaggcccgc gccggccccc
     4861 tcgcacggac ttgtgggaga agctcggcta ctcccctgcc ccggttaatt tgcatataat
     4921 atttcctagt aactatagag gcttaatgtg cgataaaaga cagataatct gttcttttta
     4981 atactagcta cattttacat gataggcttg gatttctata acttcgtata gcatacatta
     5041 tacgaagtta taaacagcac aaaaggaaac tcaccctaac tgtaaagtaa ttgtgtgttt
     5101 tgagactata agtatccctt ggagaaccac cttgttggac caggatgggc accacccgtt
     5161 taagagctaa gctggaaaca gcatagcaag tttaaataag gctagtccgt tatcaacttg
     5221 aaaaagtggc accgagtcgg tgcgctcacc tattagcggc taaggttttt ttctcgagta
     5281 ctaggatcca ttaggcggcc gcgtggataa ccgtattacc gccatgcatg tgcccgtcag
     5341 tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga
     5401 accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc
     5461 cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt
     5521 ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg ttcccgcggg
     5581 cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac ctggctgcag
     5641 tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc gaggccttgc
     5701 gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg ctggggccgc
     5761 cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc
     5821 atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag tcttgtaaat
     5881 gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc
     5941 cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc accgagaatc
     6001 ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc gccgccgtgt
     6061 atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg agcggaaaga
     6121 tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag
     6181 cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca
     6241 tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc gcgcttttgg
     6301 agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc ccacactgag
     6361 tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt ggaatttgcc
     6421 ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca aagttttttt
     6481 cttccatttc aggtgtcgtg agctagccca ccatgaccga gtacaagccc acggtgcgcc
     6541 tcgccacccg cgacgacgtc ccccgggccg tacgcaccct cgccgccgcg ttcgccgact
     6601 accccgccac gcgccacacc gtcgacccgg accgccacat cgagcgggtc accgagctgc
     6661 aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg
     6721 gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg
     6781 agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg
     6841 aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg
     6901 tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg
     6961 cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct
     7021 tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca
     7081 cctggtgcat gacccgcaag cccggtgccg gatcgggaga gggcagagga agtctgctaa
     7141 catgcggtga cgtcgaggag aatcctggcc caccggtcgc caccagcgag ctgattaagg
     7201 agaacatgca catgaagctg tacatggagg gcaccgtgga caaccatcac ttcaagtgca
     7261 catccgaggg cgaaggcaag ccctacgagg gcacccagac catgagaatc aaggtggtcg
     7321 agggcggccc tctccccttc gccttcgaca tcctggctac tagcttcctc tacggcagca
     7381 agaccttcat caaccacacc cagggcatcc ccgacttctt caagcagtcc ttccctgagg
     7441 gcttcacatg ggagagagtc accacatacg aagacggggg cgtgctgacc gctacccagg
     7501 acaccagcct ccaggacggc tgcctcatct acaacgtcaa gatcagaggg gtgaacttca
     7561 catccaacgg ccctgtgatg cagaagaaaa cactcggctg ggaggccttc accgagacgc
     7621 tgtaccccgc tgacggcggc ctggaaggca gaaacgacat ggccctgaag ctcgtgggcg
     7681 ggagccatct gatcgcaaac atcaagacca catatagatc caagaaaccc gctaagaacc
     7741 tcaagatgcc tggcgtctac tatgtggact acagactgga aagaatcaag gaggccaaca
     7801 acgagaccta cgtcgagcag cacgaggtgg cagtggccag atactgcgac ctccctagca
     7861 aactggggca caagcttaat taagaattcg tcgagggacc taataacttc gtatagcata
     7921 cattatacga agttatacat gtttaagggt tccggttcca ctaggtacaa ttcgatatca
     7981 agcttatcga taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     8041 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     8101 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     8161 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     8221 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     8281 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     8341 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
     8401 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     8461 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     8521 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     8581 atcgataccg tcgacctcga tcgagaccta gaaaaacatg gagcaatcac aagtagcaat
     8641 acagcagcta ccaatgctga ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt
     8701 tttccagtca cacctcaggt acctttaaga ccaatgactt acaaggcagc tgtagatctt
     8761 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa
     8821 gatatccttg atctgtggat ctaccacaca caaggctact tccctgattg gcagaactac
     8881 acaccagggc cagggatca
//
