LOCUS       sequence-292066-       10664 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  doxycycline-inducible expression of mouse Myb in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10664)
  AUTHORS   Rosa FF, Pires CF, Kurochkin I, Ferreira AG, Gomes AM, Palma LG, 
            Shaiv K, Solanas L, Azenha C, Papatsenko D, Schulz O, Reis e Sousa 
            C, Pereira CF
  TITLE     Direct reprogramming of fibroblasts into antigen-presenting 
            dendritic cells.
  JOURNAL   Sci Immunol. 2018 Dec 7;3(30). pii: 3/30/eaau4292. doi: 
            10.1126/sciimmunol.aau4292.
  PUBMED    30530727
REFERENCE   2  (bases 1 to 10664)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Immunol."; date: "2018-12-7"; volume: "3(30). pii: 3/30/eaau4292. 
            doi"; pages: " 10.1126/sciimmunol.aau4292"
COMMENT     Sequence Label: pFUW-tetO-Myb
FEATURES             Location/Qualifiers
     source          1..10664
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    277..394
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(451..469,470..492,493..511,512..534,535..553,554..576,
                     577..595,596..618,619..637,638..660,661..679,680..702,
                     703..721)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        754..792
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     789..813
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    3193..3781
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3246..3266)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3664..3675)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3784..3800)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3785..3801)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4306..4486
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4512..4529)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4518..4742
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4788..5216
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4875..4894)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5085..5106
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5225..5245)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5230..5559
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5410..5545
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5472..5491
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        5607..5654
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5673..6047
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6177..6298
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6214..6233)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6268..6287
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6347..6363)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6347..6363)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6360..6382)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6371..6387
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6395..6401,6402..6419,6420..6425))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6440..6461
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6578..6595)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6749..7337)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6829..6848)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7508..8299,8300..8368))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8131..8150
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8369..8473)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8548..8567)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8739..9118
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9120..9318
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             9336..9516
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9563..9688
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10181..10414
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10599..10643
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
       61 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
      121 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa
      181 ggtggagaga gagacagaga cagatccatt cgattagtga acggatcggc actgcgtgcg
      241 ccaattctgc agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg
      301 ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga
      361 attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga
      421 tccagtttgg ttagatctcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
      481 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta
      541 tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt
      601 gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac
      661 tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag
      721 aaaagtgaaa gtcgagctcg gtacccgggt cgaggtaggc gtgtacggtg ggaggcctat
      781 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
      841 gacctccata gaagacaccg ggaccgatcc agcctccgcg gccccgaatt gatccatggc
      901 ccggagaccc cgacacagca tctacagtag cgatgaagat gatgaagaca ttgagatgtg
      961 tgaccatgac tacgatgggc tgctgcccaa atctggaaag cgtcacttgg ggaaaactag
     1021 gtggacaagg gaagaggatg agaagctgaa gaagctggtg gaacagaacg gaacagacga
     1081 ctggaaagtc attgccaatt atctgcccaa ccggacagat gtgcagtgcc aacaccggtg
     1141 gcagaaagtg ctgaaccctg aactcatcaa aggtcactgg accaaagaag aagatcagag
     1201 agtcatagag cttgtccaga aatatggtcc gaagcgttgg tctgttattg ccaagcactt
     1261 aaaagggaga attggaaagc agtgtcggga gaggtggcac aaccatttga atccagaagt
     1321 taagaaaacc tcctggacag aagaggagga cagaatcatt taccaggcac acaagcgtct
     1381 ggggaacaga tgggcagaga tcgcaaagct gctgcccgga cggactgata atgctatcaa
     1441 gaaccactgg aattccacca tgcgtcgcaa ggtggaacag gaaggctacc tgcaggagcc
     1501 ttccaaagcc agccagacgc cagtggccac gagcttccag aagaacaatc atttgatggg
     1561 gtttgggcat gcctcacctc catctcagct ctctccaagt ggccagtcct ccgtcaacag
     1621 cgaatatccc tattaccaca tcgccgaagc acaaaacatc tccagtcacg ttccctatcc
     1681 tgtcgcattg catgttaata tagtcaacgt ccctcagccg gctgcggcag ccatccagag
     1741 acactataac gacgaagacc ctgagaagga aaagcgaata aaggagctgg agttgctcct
     1801 gatgtcaaca gagaacgagc tgaagggaca gcaggcatta ccaacacaga accacacttg
     1861 cagctacccc gggtggcaca gcacctccat tgtggaccag accagacctc atggggatag
     1921 tgcacctgtt tcctgtttgg gagaacacca tgccacccca tctctgcctg cagatcccgg
     1981 ctccctacct gaagaaagtg cctcaccagc aaggtgcatg atcgtccacc agggcaccat
     2041 tctggacaat gttaagaacc tcttagaatt tgcagaaaca ctccagttta tagattctga
     2101 ttcttcgtgg tgtgatctca gcagttttga attctctgaa gaagcggcag ctttttcacc
     2161 tagccagcag cccacaggca aagccttcca gcttcagcaa agagagggcc atgggactag
     2221 atctgcagga gagcctagcc tgagggtgac caggcgagtg ctgagcgagg catccctcgg
     2281 cccagactca ccccaagcga ggcacagcaa ggttccgctg gtcgtcctac gaaaaaggcg
     2341 gggccaggcc agccccctag ccgctggaga gcctagcccc tccctctttg ctgacgtcat
     2401 cagctcaact ctcaagcgtt cccctgtcaa aagcctaccc ttctctccct cgcagttctt
     2461 gaacacttcc agcaaccatg aaagctcggg cttagatgca cctaccttac cctccactcc
     2521 tctcattggt cacaaactga caccatgtcg agaccagact gtgaaaaccc agaaggaaaa
     2581 ttccatcttt agaactccag ctatcaaaag gtcaatcctc gaaagctctc ctcgaactcc
     2641 cacaccattc aaacatgccc ttgcagctca agaaattaaa tacggtcccc tgaagatgct
     2701 acctcagacc ccctcccatg cagtggagga cctacaagat gtgattaagc gggaatcgga
     2761 tgaatctgga attgttgctg agtttcaaga gagtggacca ccgttactga aaaaaatcaa
     2821 gcaggaggtg gagtcgccaa ctgagaaatc gggaaacttc ttctgctcaa accactgggc
     2881 agagaacagc ctgagcaccc agctgttctc gcaggcgtct cctgtggcag atgccccaaa
     2941 tattcttaca agctctgttt taatgacacc tgtatcagaa gatgaagaca atgtcctcaa
     3001 agcctttacc gtacctaaga acaggcccct ggtgggtccc ttgcagccat gcagtggtgc
     3061 ctgggagcca gcatcctgtg ggaagacaga ggaccagatg acggcctccg gtccggctcg
     3121 gaaatacgtg aacgcgttct cagctcgaac tctggtcatg tgagcggcca attcgatatc
     3181 aagcttatcg ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     3241 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     3301 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     3361 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     3421 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     3481 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     3541 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
     3601 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     3661 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     3721 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     3781 catcgatacc gtcgacctcg agacctagaa aaacatggag caatcacaag tagcaataca
     3841 gcagctacca atgctgattg tgcctggcta gaagcacaag aggaggagga ggtgggtttt
     3901 ccagtcacac ctcaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc
     3961 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat
     4021 atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattggca gaactacaca
     4081 ccagggccag ggatcagata tccactgacc tttggatggt gctacaagct agtaccagtt
     4141 gagcaagaga aggtagaaga agccaatgaa ggagagaaca cccgcttgtt acaccctgtg
     4201 agcctgcatg ggatggatga cccggagaga gaagtattag agtggaggtt tgacagccgc
     4261 ctagcatttc atcacatggc ccgagagctg catccggact gtactgggtc tctctggtta
     4321 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     4381 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     4441 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagggc ccgtttaaac
     4501 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     4561 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     4621 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     4681 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
     4741 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag
     4801 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     4861 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     4921 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     4981 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     5041 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     5101 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     5161 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt
     5221 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     5281 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     5341 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     5401 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     5461 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag
     5521 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata
     5581 tccattttcg gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca
     5641 tagtataata cgacaaggtg aggaactaaa ccatggccaa gttgaccagt gccgttccgg
     5701 tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct
     5761 cccgggactt cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca
     5821 tcagcgcggt ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg
     5881 gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct
     5941 ccgggccggc catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg
     6001 acccggccgg caactgcgtg cacttcgtgg ccgaggagca ggactgacac gtgctacgag
     6061 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
     6121 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact
     6181 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     6241 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     6301 atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
     6361 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     6421 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     6481 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     6541 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     6601 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     6661 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     6721 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     6781 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     6841 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     6901 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     6961 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     7021 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     7081 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     7141 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     7201 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     7261 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     7321 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     7381 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     7441 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     7501 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     7561 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     7621 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     7681 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     7741 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     7801 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     7861 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     7921 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     7981 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     8041 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     8101 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     8161 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     8221 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     8281 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     8341 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     8401 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     8461 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc
     8521 tcccgatccc ctatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     8581 gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc
     8641 tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg ttaggcgttt
     8701 tgcgctgctt cgcgatgtac gggccagata tacgcgttga cattgattat tgactagtta
     8761 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
     8821 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
     8881 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
     8941 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
     9001 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
     9061 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
     9121 gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc
     9181 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt
     9241 tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg
     9301 ggaggtctat ataagcagcg cgttttgcct gtactgggtc tctctggtta gaccagatct
     9361 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     9421 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     9481 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa
     9541 agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac
     9601 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta
     9661 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg
     9721 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg
     9781 ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg
     9841 ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag aagaacttag
     9901 atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga
     9961 caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca
    10021 gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg
    10081 aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa
    10141 agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt
    10201 tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca
    10261 gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc
    10321 aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg
    10381 ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac
    10441 tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga
    10501 tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa
    10561 tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg
    10621 aattagataa atgggcaagt ttgtggaatt ggtttaacat aaca
//
