LOCUS       sequence-292253-        9064 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Light-controlled tri-directional targeting of endogenous proteins 
            (cytoplasm-shifted).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9064)
  AUTHORS   Redchuk TA, Karasev MM, Verkhusha PV, Donnelly SK, Hulsemann M, 
            Virtanen J, Moore HM, Vartiainen MK, Hodgson L, Verkhusha VV
  TITLE     Optogenetic regulation of endogenous proteins.
  JOURNAL   Nat Commun. 2020 Jan 30;11(1):605. doi: 10.1038/s41467-020-14460-4.
  PUBMED    32001718
REFERENCE   2  (bases 1 to 9064)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-01-30"; volume: "11(1):605. doi"; pages: " 
            10.1038/s41467-020-14460-4"
COMMENT     Sequence Label: pQP-CiRISB
FEATURES             Location/Qualifiers
     source          1..9064
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        113..416
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        417..620
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     571..591
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      668..677
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    2962..3018
                     /label=MCS
                     /note="multiple cloning site"
     misc_feature    3020..3594
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3044..3596
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3188..3205)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3415..3434
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3623..3964
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid 
                     antibody (Rothbauer et al., 2008)"
                     /label=GFP nanobody
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
     CDS             4028..4735
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4173..4191)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4441..4460
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             5240..5254
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     polyA_signal    5904..6025
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5941..5960)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5995..6014
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(6032..6487)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6169..6190)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6381..6400
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6514..6618
                     /gene="bla"
                     /label=AmpR promoter
     promoter        6620..6977
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6620..6811
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6643..6663)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6828..6963
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7012..7806
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7012..7806
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7066..7085)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7676..7695
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(7994..8013)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    8038..8085
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      8414..9002
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8903..8922
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgccatgc attagttatt
       61 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
      121 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
      181 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
      241 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
      301 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
      361 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
      421 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
      481 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
      541 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
      601 aggtctatat aagcagagct ggtttagtga accgtcagat ccgctagcgc taccggactc
      661 agatctacca tggtggcagg tcatgcctct ggcagccccg cattcgggac cgccgatctt
      721 tcgaattgcg aacgtgaaga gatccacctc gccggctcga tccagccgca tggcgcgctt
      781 ctggtcgtca gcgagccgga tcatcgcatc atccaggcca gcgccaacgc cgcggaattt
      841 ctgaatctcg gaagcgtgct cggcgttccg ctcgccgaga tcgacggcga tctgttgatc
      901 aagatcctgc cgcatctcga tcccaccgcc gaaggcatgc cggtcgcggt gcgctgccgg
      961 atcggcaatc cctccacgga gtacgacggt ctgatgcatc ggcctccgga aggcgggctg
     1021 atcatcgagc tcgaacgtgc cggcccgccg atcgatctgt ccggcacgct ggcgccggcg
     1081 ctggagcgga tccgcacggc gggctcgctg cgcgcgctgt gcgatgacac cgcgctgctg
     1141 tttcagcagt gcaccggcta cgaccgggtg atggtgtatc gcttcgacga gcagggccac
     1201 ggcgaagtgt tctccgagcg ccacgtgccc gggctcgaat cctatttcgg caaccgctat
     1261 ccgtcgtcgg acattccgca gatggcgcgg cggctgtacg agcggcagcg cgtccgcgtg
     1321 ctggtcgacg tcagctatca gccggtgccg ctggagccgc ggctgtcgcc gctgaccggg
     1381 cgcgatctcg acatgtcggg ctgcttcctg cgctcgatgt cgccgatcca tctgcagtac
     1441 ctgaagaaca tgggcgtgcg cgccaccctg gtggtgtcgc tggtggtcgg cggcaagctg
     1501 tggggcctgg ttgcctgtca ccattatctg ccgcgcttca tccatttcga gctgcgggcg
     1561 atctgcgaac tgctcgccga agcgatcgcg acgcggatca ccgcgcttga gagcttcgcg
     1621 cagagccagt cggagctgtt cgtgcagcgg ctcgaacagc gcatgatcga agcgatcacc
     1681 cgtgaaggcg attggcgcgc agcgattttc gacaccagcc aatcgatcct gcagccgctg
     1741 cacgccgacg gttgcgcgct ggtgtacgaa gaccagatca ggaccatcgg tgacgtacct
     1801 tccacgcagg atgttcgcga gatcgccggg tggctcgatc gccagccacg tgcggcggtg
     1861 acctcgaccg cgtcgctcgg tctcgacgtg ccggagctcg cgcatctgac gcggatggcg
     1921 agcggcgtgg tcgcggcgcc gatttcggat catcgcggcg agtttctgat gtggttccgc
     1981 cccgagcgcg tccacaccgt tacctggggc ggcgatccga agaagccgtt cacgatgggc
     2041 gatacaccgg cggatctgtc gccgcggcgc tccttcgcca aatggcatca ggttgtcgaa
     2101 ggcacgtccg atccgtggac ggccgccgat ctcgccgcgg ctcgcaccat cggtcagacc
     2161 gtcgccgaca tcgtgctgca attccgcgcg gtgcggacac tgatcgcccg cgaacagtac
     2221 gaacagtttt cgtcccaggt gcacgcttcg atgcagccgg tgctgatcac cgacgccgaa
     2281 ggccgcatcc tgctgatgaa cgactcgttc cgcgacatgt tgccggcggg gtcgccatcc
     2341 gccgtccatc tcgacgatct cgccgggttc ttcgtcgaat cgaacgattt cctgcgcaac
     2401 gtcgccgaac tgatcgatca cggccgcggg tggcgcggcg aagttctgct gcgcggcgca
     2461 ggtaatcgcc cgttgccgct ggcagtgcgc gccgatccgg tgacgcgcac ggaggaccag
     2521 tcgctcggct tcgtgctgat cttcagcgac gctaccgatc gtcgcaccgc agatgccgca
     2581 cgcacgcgtt tccaggaagg cattcttgcc agcgcacgtc ccggcgtgcg gctcgactcc
     2641 aagtccgacc tcttgcacga gaagctgctg tccgcgctgg tcgagaacgc gcagcttgcc
     2701 gcattggaaa ttacttacgg cgtcgagacc ggacgcatcg ccgagctgct cgaaggcgtt
     2761 cgccagtcga tgctgcgcac cgccgaagtg ctcggccatc tggtgcagca cgcggcgcgc
     2821 acggccggca gcgacagctc gagcaatggc tcgcagaaca agaaggctag cagtgctggc
     2881 ggtagtgctg gtagtgctgg tcgtacggcc agcggtaaaa agaagaaaaa gaagtcaaag
     2941 acaaagtgtg taattatgta aagatctcga gctcaagctt cgaattctgc agtcgacggt
     3001 accgcgggcc cgggatccgc ccctctccct cccccccccc ctaacgttac tggccgaagc
     3061 cgcttggaat aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct
     3121 tttggcaatg tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt
     3181 ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct
     3241 ctggaagctt cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc
     3301 ccacctggcg acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag
     3361 gcggcacaac cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc
     3421 tcctcaagcg tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga
     3481 tctgatctgg ggcctcggta cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt
     3541 ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg ataatatggc
     3601 cacaaaccgg tccatggatc aagtccaact ggtggagtct ggtggcgctt tggtgcagcc
     3661 aggtggctct ctgcgtttgt cctgtgccgc ttctggcttc ccagtgaacc gctattccat
     3721 gcgctggtat cgccaggctc caggcaaaga gcgtgagtgg gtagccggta tgtccagcgc
     3781 gggtgatcgt agctcctatg aagactccgt gaagggccgt ttcaccatca gccgtgacga
     3841 tgcccgtaac acggtgtatc tgcaaatgaa cagcttgaaa cctgaagata cggccgtgta
     3901 ttactgtaat gtgaacgtgg gcttcgagta ttggggccaa ggcacccagg tcaccgtctc
     3961 cagcggtggc ggtggatctg accggtccat gggaattgat ctctcaggat taacactgca
     4021 gactagtatg gtgagcaagg gcgaggagga taacatggcc atcatcaagg agttcatgcg
     4081 cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg
     4141 cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc
     4201 cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggcctacgt
     4261 gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg gcttcaagtg
     4321 ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct
     4381 gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg
     4441 ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc tccgagcgga tgtaccccga
     4501 ggacggcgcc ctgaagggcg agatcaagca gaggctgaag ctgaaggacg gcggccacta
     4561 cgacgctgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc ccggcgccta
     4621 caacgtcaac atcaagttgg acatcacctc ccacaacgag gactacacca tcgtggaaca
     4681 gtacgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt acaagagtgc
     4741 tggtgggtcc gccggcggga gtgctggcgg tagtgccggc ggtagttcta gaggcaagaa
     4801 catgcaggcg gtcaccgagc tgcattcccg gctgatcgct gcgcagcagg cgatggagcg
     4861 cgactattgg cggttgcgtg aattggagac tcgctaccgc ctggtgttcg acgctgccgc
     4921 cgatgcggtg atgatcgtct ccgccggcga catgcgcatc gtcgaagcca accgggcggc
     4981 ggtgaatgcg atcagccgcg tcgagcgcgg caatgacgac cttgcggggc gtgatttcct
     5041 cgccgaagtg gcggctgccg atcgcgatgc ggtgcgcgac atgctggccc aggtgcgtca
     5101 gcgcggcacc gcactcagcg tcctcgttca tctcggccgt tacgaccgcg cctggatgct
     5161 gcgcggttcg ctgatgtcgt ccgagcgtcg tcaggttttc ctgctgcact tcaccccggt
     5221 gaccacgact cccgcgatcg acgacgacga caaaggtgtc gttgcttctg ctgccgacgg
     5281 tgcagagggc gcctcagacg acgcagagga tgaattcccg gctagcggcg ccggcgcctt
     5341 tttggctact acacttgaac gtattgagaa gaactttgtc attactgacc caaggttgcc
     5401 agataatccc attatattcg cgtccgatag tttcttgcag ttgacagaat atagccgtga
     5461 agaaattttg ggaagaaact gcaggtttct acaaggtcct gaaactgatc gcgcgacagt
     5521 gagaaaaatt agagatgcca tagataacca aacagaggtc actgttcagc tgattaatta
     5581 tacaaagagt ggtaaaaagt tctggaacct ctttcacttg cagcctatgc gagatcagaa
     5641 gggagatgtc cagtacttta ttggggttca gttggatgga actgagcatg tccgagatgc
     5701 tgccgagaga gagggcgtta tgctggccaa gaagaccgcc gagaacatcg acatggctgc
     5761 caagagggtc aagttggact gagcggccgc gactctagat cataatcagc cataccacat
     5821 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
     5881 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa
     5941 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     6001 tgtccaaact catcaatgta tcttaaggcg taaattgtaa gcgttaatat tttgttaaaa
     6061 ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa
     6121 atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac
     6181 aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag
     6241 ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt
     6301 aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg
     6361 gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca
     6421 agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag
     6481 ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     6541 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     6601 tattgaaaaa ggaagagtcc tgaggcggaa agaaccagct gtggaatgtg tgtcagttag
     6661 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     6721 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     6781 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     6841 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     6901 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     6961 gcctaggctt ttgcaaagat cgatcaagag acaggatgag gatcgtttcg catgattgaa
     7021 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac
     7081 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg
     7141 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaagacgag
     7201 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt
     7261 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg
     7321 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg
     7381 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga
     7441 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag
     7501 gggctcgcgc cagccgaact gttcgccagg ctcaaggcga gcatgcccga cggcgaggat
     7561 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
     7621 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg
     7681 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt
     7741 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
     7801 ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac
     7861 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
     7921 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccta
     7981 gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc gctatgacgg
     8041 caataaaaag acagaataaa acgcacggtg ttgggtcgtt tgttcataaa cgcggggttc
     8101 ggtcccaggg ctggcactct gtcgataccc caccgagacc ccattggggc caatacgccc
     8161 gcgtttcttc cttttcccca ccccaccccc caagttcggg tgaaggccca gggctcgcag
     8221 ccaacgtcgg ggcggcaggc cctgccatag cctcaggtta ctcatatata ctttagattg
     8281 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     8341 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     8401 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     8461 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     8521 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     8581 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     8641 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     8701 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     8761 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     8821 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     8881 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     8941 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     9001 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     9061 tgtt
//
