LOCUS       sequence-292390-       10846 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  all-in-one CRISPRi vector for targeting B. burgdorferi ftsI.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10846)
  AUTHORS   Takacs CN, Scott M, Chang Y, Kloos ZA, Irnov I, Rosa PA, Liu J, 
            Jacobs-Wagner C
  TITLE     A CRISPR interference platform for selective downregulation of gene 
            expression in Borrelia burgdorferi.
  JOURNAL   Appl Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20.
  PUBMED    33257311
REFERENCE   2  (bases 1 to 10846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol. 2020 Nov 30. pii: AEM.02519-20. doi: 
            10.1128/AEM.02519-20."
COMMENT     Sequence Label: pBbdCas9S_Psyn-sgRNAftsI
FEATURES             Location/Qualifiers
     source          1..10846
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3323..3357
                     /label=J23119(SpeI) promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119) modified to end with an SpeI site"
     misc_RNA        3379..3454
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        join(4897..4911,4912..4917,4918..4934,4935..4941)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    4917..4933
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    4949..4965
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             4984..4995
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             join(5002..5028,5029..5031,5032..7518,7519..7521,
                     7522..9108)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             9278..10069
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      10192..10780
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10681..10700
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcactag atccccggca agttcatcct tagggtttcc atatctctta aaaagtcaat
       61 ttctgcttga ttaatttctt gtggattatt gtttttgcgg tttttcattt tttatctcct
      121 tgagtattga attttttctt atatataaat atatagcaaa aactattttt gccaactttt
      181 ttacaaaaaa ttttttatta aaacacttag ggctttgcta aattctcttt taaaagaact
      241 tagtaaagcc ctatggattt aagaactgat tatacttacg tagtaaaaat actacagata
      301 ttaatagtat agaccatatc aaaatcataa tcaatttata tttatctaaa attgtagtcc
      361 atacaaaata caaaaaaatt aggtttttct aaattctatt ttaaaagaac ttagtaacac
      421 ctaatctcta aattacttac aaaattctaa aattagattt ttagttcttc atatttcttt
      481 aaaagttttt taagaaaatc tttttgattt tcaaaaattt catgcatcat aaaactagta
      541 aatttggatt tttttttgta aaaatcataa ctttcttggg ttttaagttg aaatcttaat
      601 ggttttattg gattttgcct agattttttt aacgccggac tttctttatc ttttataaaa
      661 ttataagaat ttttaatacc attttctatt aaataatctt cttttataag cccctcttgc
      721 agtgcagttg ctaatcttaa atacttatac gcttgagttt ttgctacatc aaagtctttt
      781 ataaaatcaa gaaaagtttt ataaccatca aatttataat attgattatc ttttatttct
      841 tttaaaattt taatattttc caccctgtgg aatatttctt ttttgaaatt gtcatttaac
      901 ttttccttta gtgtcttgta ataagaaatt ttactttcat taatcattaa attttgttca
      961 acataagtat ctatcttttt tgttaaaact ttaacaacac gttttttttc cataaaaatt
     1021 ttcctttatt tttgcaacat tttccttaat tgatataatt gttactattt tagatttttt
     1081 aatatccact taagaattaa tccctcacta gaatattttt tattatagaa ttcatataca
     1141 tgtttttcta aatctataaa tttatcaaca attgctttat tatagggtgt atctatcttt
     1201 tcttttctta ataatcgtct catattcttt aaataacaac aaacactgcc ggttttaaac
     1261 ctaaattcta tgtaatatat ttttgaaaaa gtatatacat tttcaacccc gtttatttca
     1321 tactttacaa aaacattatt tattggtttt ctataaccat agtaaatgcc taaaaatttg
     1381 tccccgtctt tcataggata tagataaagt ttttgaatct ctgatttatc taataatttt
     1441 tcaaaaaaaa ttatacaccg cttttgtttg ttactaacta ctctatgcaa tgcctttaat
     1501 atttttgtgt gatatatatt tttatcacct tttttttctt tcagcaggaa gatactttct
     1561 tttggaaatt taattttttg ttcatttatt cttgcagcct taagtccgat tttcatattt
     1621 aatccttata tagcctttct atttaattct tttaaactca ataaactttt ttggtttttc
     1681 attagttcta ataattcgca ataatatgtg caaaacactt tattgtattc tattttcttt
     1741 tggttattca aatattcttt tataattggc tttagaagtt caatatttac ttcttttttt
     1801 agttgttcaa taaggatatt gaaaatgttt gtttttaaat cttgataatt ttgttgagaa
     1861 ttttcttttt ttctttcaat tgacttctct aatttacgct ttatattgtt taaatcgcta
     1921 tatttatgac tttcaataat aaaatgaggc ttaaatttgt aattttcgta tattttttgg
     1981 aaacttgttt ctaattgttt agaattatat tcattttttt caaattcttt ttgagtgttg
     2041 catagaattt cctttaattt ttcttgtttt tctttcatac aagatttagt aaaatgtata
     2101 tttttatttt ttagtaattt aatttctatt atttttatga ttttaagtat tttgattata
     2161 ttatctttat caatatttaa attgaataaa attgtaagaa aaattgaaag aattttttta
     2221 cataaaaagt tacatttatt gaagtaattt cttacttgat acttttctat ttcattaatc
     2281 tttctttctt cttttatatt atttttatta tttaaacact cctttaaatc tacactccca
     2341 tttttagtaa atctatcttt aaggcccctt gaaactctat tttgaaatct taaatctttt
     2401 ttttcttgaa agtatttata gattttttgg taacattctt tttttgaaaa attaagttga
     2461 tagtatattt cggtccccaa atttacccct aagtgtttgt gataattagt tgtgacttta
     2521 aattcttttt ctaatttgta aaggtaattt tgcagtgttt ttagtttaac gggtgtttga
     2581 ctatttcttt taaggttttc attaaagtaa tagagtatgt ttttttgtgt gtatttctta
     2641 tatgttttat ttatgtacgc tagtgttgag ataagaacta ctaatttata ctgtaattta
     2701 ttgtagcaag ttgtggtttt actggttttg tttatattag ataagttttc catttatagc
     2761 tccttgaata ccggattttt tatctataaa gtatatatat cacaattttg atttaaaagt
     2821 aaattctttt tttaaaaata caaaaatttt ttataaatta tatagaccat gatattgtat
     2881 taatactaat tgattttaat ttaaggttga actaaaataa acatagtttt gtagaaaata
     2941 atttttcatt attttgctac ttgatattgg atcgtaaaaa tattagggct ttacaaagtt
     3001 cttttaaaag agaatttagt aaagccctaa gtcttttaat aaaaaatttt ttgtaaaaaa
     3061 gttggcaaaa atagtttttg ctatatattt atatataaga aaaaattcaa tactcaagga
     3121 gataaaaaat ggaaaactta aacaataatc cacaaaaaat taatcaaata aataagcaaa
     3181 ctaattttat tggttttgaa aattttatag aacttgataa tcaagttaat caaactaaag
     3241 aaaaaagtaa attaagtaag ataggtaaga aattacccgg aataagcagt caagtctagt
     3301 gcggccggaa ttctaaagat ctttgacagc tagctcagtc ctaggtataa tactagtgag
     3361 tgttattaag cattctttgt tttagagcta gaaatagcaa gttaaaataa ggctagtccg
     3421 ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt tggcgcgcca aataatgccc
     3481 gtgtccctgt tgaataggga cacggttttt taacaatttt tttattccta ggccttagtg
     3541 gccggtaccc ggggatcctt attactggcc gctttctagc ctggatactt gtctggctag
     3601 ttgcattaga gagtcagcta gagccctagg gctagctgtt tgtgtattcg gagccagtgt
     3661 agtttttctt tttactaacg atacgggtaa taattgattg ccttttacag cctgaccttg
     3721 agacaattgc aaaagtctgt ccacagaggt ttgacctaat aatctaaaat cttgtttgat
     3781 agtagtaagt ggaggtatgt agcaactaga atcttctgta tcatcataac ccacaactga
     3841 aatatcagcc ccaactctaa gtccactttc agtaatagct cgcatagctc ctaaagccat
     3901 ttgatcatta gcaactaaca tagcagtagg gacaatgcct tcatttaaca tttgcattgt
     3961 ttgctgaaat cctgacatag cagaccaatc tccttctctt tctgctattg gttgtatttg
     4021 atttcttgtt aaatacttgt gccatcctgc taatctcaat ctggcactta ctgaacttaa
     4081 gggacctgca agtagtgcaa tttgttgatg gcctaatgca acaaggtgtt caactcctaa
     4141 ccttgttcca tcttcgtgag aaaaaattat agagtttatt ggggtttggt ccgacacatc
     4201 caaaaacaat gctggaacat tagtacatgc tgcttcaact gcaatcgcat cttggtcatc
     4261 aagaggataa ttaattataa gtccactaac acgttgagct aataaattat gtactgctgc
     4321 cttacaagcc tctacacctg atctctcaac cattgataca acaacgcttg caccaagttg
     4381 atcagctctc gatttaattg cggctacaat ttgactaggc gcatgtaatg caagtgatga
     4441 tgtggcaact ccaattaaaa gtgattgttt ccccgcaagt tgctgagcaa cgcgatttgg
     4501 tatataattc aactctgcca tagctgcttc aaccttctct cttgtttttg cagatacatg
     4561 acttgcttgg ttcacaactc tcgaaaccgt ttggtagcta accccggcat attcagcaac
     4621 atcatacaaa gtaacaggtt tcatggatca ttcctccatg ataaaattta aatttctgac
     4681 tttggcaaat ccttttgcct gttaaaaaga atagcacaaa aattaaaaaa aaagaaacgc
     4741 aaatagcaat agcattaaaa ataattatta ttatttattt ctttgattaa aatcaaaagc
     4801 ctttaatgta ttttcttgat ctttcatgcg cttaacggta aaatccaagg tattaagttc
     4861 accttccact tttctctctc tatcttctct agaaaatcat aaaaaattta tttgctttgt
     4921 gagcggataa caattataat agattcaatt gtgagcggat aacaatttca cacagaattc
     4981 attaaagagg agaaattaca tatggataag aaatactcaa taggcttagc tatcggcaca
     5041 aatagcgtcg gatgggcggt gatcactgat gaatataagg ttccgtctaa aaagttcaag
     5101 gttctgggaa atacagaccg ccacagtatc aaaaaaaatc ttataggggc tcttttattt
     5161 gacagtggag agacagcgga agcgactcgt ctcaaacgga cagctcgtag aaggtataca
     5221 cgtcggaaga atcgtatttg ttatctacag gagatttttt caaatgagat ggcgaaagta
     5281 gatgatagtt tctttcatcg acttgaagag tcttttttgg tggaagaaga caagaagcat
     5341 gaacgtcatc ctatttttgg aaatatagta gatgaagttg cttatcatga gaaatatcca
     5401 actatctatc atctgcgaaa aaaattggta gattctactg ataaagcgga tttgcgctta
     5461 atctatttgg ccttagcgca tatgattaag tttcgtggtc attttttgat tgagggagat
     5521 ttaaatcctg ataatagtga tgtggacaaa ctatttatcc agttggtaca aacctacaat
     5581 caattatttg aagaaaaccc tattaacgca agtggagtag atgctaaagc gattctttct
     5641 gcacgattga gtaaatcaag acgattagaa aatctcattg ctcagctccc cggtgagaag
     5701 aaaaatggct tatttgggaa tctcattgct ttgtcattgg gtttgacccc taattttaaa
     5761 tcaaattttg atttggcaga agatgctaaa ttacagcttt caaaagatac ttacgatgat
     5821 gatttagata atttattggc gcaaattgga gatcaatatg ctgatttgtt tttggcagct
     5881 aagaatttat cagatgctat tttactttca gatatcctaa gagtaaatac tgaaataact
     5941 aaggctcccc tatcagcttc aatgattaaa cgctacgatg aacatcatca agacttgact
     6001 cttttaaaag ctttagttcg acaacaactt ccagaaaagt ataaagaaat cttttttgat
     6061 caatcaaaaa acggatatgc aggttatatt gatgggggag ctagccaaga agaattttat
     6121 aaatttatca aaccaatttt agaaaaaatg gatggtactg aggaattatt ggtgaaacta
     6181 aatcgtgaag atttgctgcg caagcaacgg acctttgaca acggctctat tccccatcaa
     6241 attcacttgg gtgagctgca tgctattttg agaagacaag aagactttta tccattttta
     6301 aaagacaatc gtgagaagat tgaaaaaatc ttgacttttc gaattcctta ttatgttggt
     6361 ccattggcgc gtggcaatag tcgttttgca tggatgactc ggaagtctga agaaacaatt
     6421 accccatgga attttgaaga agttgtcgat aaaggtgctt cagctcaatc atttattgaa
     6481 cgcatgacaa actttgataa aaatcttcca aatgaaaaag tactaccaaa acatagtttg
     6541 ctttatgagt attttacggt ttataacgaa ttgacaaagg tcaaatatgt tactgaagga
     6601 atgcgaaaac cagcatttct ttcaggtgaa cagaagaaag ccattgttga tttactcttc
     6661 aaaacaaatc gaaaagtaac cgttaagcaa ttaaaagaag attatttcaa aaaaatagaa
     6721 tgttttgata gtgttgaaat ttcaggagtt gaagatagat ttaatgcttc attaggtacc
     6781 taccatgatt tgctaaaaat tattaaagat aaagattttt tggataatga agaaaatgaa
     6841 gatatcttag aggatattgt tttaacattg accttatttg aagataggga gatgattgag
     6901 gaaagactta aaacatatgc tcacctcttt gatgataagg tgatgaaaca gcttaaacgt
     6961 cgccgttata ctggttgggg acgtttgtct cgaaaattga ttaatggtat tagggataag
     7021 caatctggca aaacaatatt agattttttg aaatcagatg gttttgccaa tcgcaatttt
     7081 atgcagctga tccatgatga tagtttgaca tttaaagaag acattcaaaa agcacaagtg
     7141 tctggacaag gcgatagttt acatgaacat attgcaaatt tagctggtag ccctgctatt
     7201 aaaaaaggta ttttacagac tgtaaaagtt gttgatgaat tggtcaaagt aatggggcgg
     7261 cataagccag aaaatatcgt tattgaaatg gcacgtgaaa atcagacaac tcaaaagggc
     7321 cagaaaaatt cgcgagagcg tatgaaacga atcgaagaag gtatcaaaga attaggaagt
     7381 cagattctta aagagcatcc tgttgaaaat actcaattgc aaaatgaaaa gctctatctc
     7441 tattatctcc aaaatggaag agacatgtat gtggaccaag aattagatat taatcgttta
     7501 agtgattatg atgtcgatgc cattgttcca caaagtttcc ttaaagacga ttcaatagac
     7561 aataaggtct taacgcgttc tgataaaaat cgtggtaaat cggataacgt tccaagtgaa
     7621 gaagtagtca aaaagatgaa aaactattgg agacaacttc taaacgccaa gttaatcact
     7681 caacgtaagt ttgataattt aacgaaagct gaacgtggag gtttgagtga acttgataaa
     7741 gctggtttta tcaaacgcca attggttgaa actcgccaaa tcactaagca tgtggcacaa
     7801 attttggata gtcgcatgaa tactaaatac gatgaaaatg ataaacttat tcgagaggtt
     7861 aaagtgatta ccttaaaatc taaattagtt tctgacttcc gaaaagattt ccaattctat
     7921 aaagtacgtg agattaacaa ttaccatcat gcccatgatg cgtatctaaa tgccgtcgtt
     7981 ggaactgctt tgattaagaa atatccaaaa cttgaatcgg agtttgtcta tggtgattat
     8041 aaagtttatg atgttcgtaa aatgattgct aagtctgagc aagaaatagg caaagcaacc
     8101 gcaaaatatt tcttttactc taatatcatg aacttcttca aaacagaaat tacacttgca
     8161 aatggagaga ttcgcaaacg ccctctaatc gaaactaatg gggaaactgg agaaattgtc
     8221 tgggataaag ggcgagattt tgccacagtg cgcaaagtat tgtccatgcc ccaagtcaat
     8281 attgtcaaga aaacagaagt acagacaggc ggattctcca aggagtcaat tttaccaaaa
     8341 agaaattcgg acaagcttat tgctcgtaaa aaagactggg atccaaaaaa atatggtggt
     8401 tttgatagtc caacggtagc ttattcagtc ctagtggttg ctaaggtgga aaaagggaaa
     8461 tcgaagaagt taaaatccgt taaagagtta ctagggatca caattatgga aagaagttcc
     8521 tttgaaaaaa atccgattga ctttttagaa gctaaaggat ataaggaagt taaaaaagac
     8581 ttaatcatta aactacctaa atatagtctt tttgagttag aaaacggtcg taaacggatg
     8641 ctggctagtg ccggagaatt acaaaaagga aatgagctgg ctctgccaag caaatatgtg
     8701 aattttttat atttagctag tcattatgaa aagttgaagg gtagtccaga agataacgaa
     8761 caaaaacaat tgtttgtgga gcagcataag cattatttag atgagattat tgagcaaatc
     8821 agtgaatttt ctaagcgtgt tattttagca gatgccaatt tagataaagt tcttagtgca
     8881 tataacaaac atagagacaa accaatacgt gaacaagcag aaaatattat tcatttattt
     8941 acgttgacga atcttggagc tcccgctgct tttaaatatt ttgatacaac aattgatcgt
     9001 aaacgatata cgtctacaaa agaagtttta gatgccactc ttatccatca atccatcact
     9061 ggtctttatg aaacacgcat tgatttgagt cagctaggag gtgactaata aaagcttgat
     9121 cagatctcag cttttttttg aagtgcctgg cagtaagttg aaaaaatttg aaataaactt
     9181 ggactatgtt aataaaaaaa tacaaattaa cgaacaaaaa gatttgaatt attatataat
     9241 ttagttaaaa gcaattttaa atgagggagg tttccatatg agggaagcgg tgatcgccga
     9301 agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt
     9361 gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat
     9421 tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa
     9481 cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt
     9541 caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca
     9601 atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga
     9661 cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc
     9721 agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga
     9781 aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct
     9841 tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc
     9901 tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag
     9961 acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga
    10021 atttgtccac tacgtgaaag gcgagatcac caaggtagtc ggcaaataat gacgtcgccg
    10081 gagcggtcga gttctggacc gaccggctcg ggttctcatg accaaaatcc cttaacgtga
    10141 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
    10201 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
    10261 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
    10321 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
    10381 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
    10441 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
    10501 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
    10561 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
    10621 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
    10681 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
    10741 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
    10801 tttacggttc ctgggctttt gctggccttt tgctcacatg ttcttt
//
