LOCUS       sequence-292505-       14676 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses AncBE4max(with SpCas9-NG), EBNA1 and blasticidin 
            resistence gene; cloning backbone for sgRNA; Contains Epstein-Barr 
            virus oriP replication origin .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 14676)
  AUTHORS   Qi T, Wu F, Xie Y, Gao S, Li M, Pu J, Li D, Lan F, Wang Y
  TITLE     Base Editing Mediated Generation of Point Mutations Into Human 
            Pluripotent Stem Cells for Modeling Disease.
  JOURNAL   Front Cell Dev Biol. 2020 Sep 25;8:590581. doi: 
            10.3389/fcell.2020.590581. eCollection 2020.
  PUBMED    33102492
REFERENCE   2  (bases 1 to 14676)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Cell 
            Dev Biol."; date: "2020-09-25"; volume: "8:590581. doi"; pages: " 
            10.3389/fcell.2020.590581. eCollection 2020"
COMMENT     Sequence Label: epi-BE4max-NG
FEATURES             Location/Qualifiers
     source          1..14676
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(944..962)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1030..1134
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1135..1203,1204..1995)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1353..1372)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2166..2754
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2655..2674
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        3024..3337
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for mouse U6 snRNA (Das 
                     et al., 1988)"
     primer_bind     3256..3276
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     misc_RNA        3358..3433
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    3481..3598
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3667..3878
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             3940..3960
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4741..8841
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SIRPKRNSDKLIARKKDWDPKKYGGFVSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASARFLQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPRAFKYFDTTIDRKVYRSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             8872..9120
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             9151..9399
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             9442..9462
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             9471..9488
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(9511..9528)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9517..9741
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        9789..10118
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9969..10104
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             10140..10538
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    10704..10838
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10741..10760)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10795..10814
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      11478..13267
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 cccccccccc cccccccccc cccccccccc cccccccccc cccccccccc cccccccccc
       61 cccccccccc cccccccccc yccccyccct ccycgmcccc ggcctccact acctcctcga
      121 ccccggcctc cactgcctcc tcgaccccgg cctccacctc ctgctcctgc ccctcctgct
      181 cctgcccctc ctcctgctcc tgcccctcct gctcctgccc ctcccgctcc tgctcctgct
      241 cctgttccac cgtgggtccc tttgcagcca atgcaacttg gacgtttttg gggtctccgg
      301 acaccatctc tatgtcttgg ccctgatcct gagccgcccg gggctcctgg tcttccgcct
      361 cctcgtcctc gtcctcttcc ccgtcctcgt ccatggttat caccccctct tctttgaggt
      421 ccactgccgc cggagccttc tggtccagat gtgtctccct tctctcctag gccatttcca
      481 ggtcctgtac ctggcccctc gtcagacatg attcacacta aaagagatca atagacatct
      541 ttattagacg acgctcagtg aatacaggga gtgcagactc ctgccccctc caacagcccc
      601 cccaccctca tccccttcat ggtcgctgtc agacagatcc aggtctgaaa attccccatc
      661 ctccgaacca tcctcgtcct catcaccaat tactcgcagc ccggaaaact cccgctgaac
      721 atcctcaaga tttgcgtcct gagcctcaag ccaggcctca aattcctcgt cccccttttt
      781 gctggacggt agggatgggg attctcggga cccctcctct tcctcttcaa ggtcaccaga
      841 cagagatgct actggggcaa cggaagaaaa gctgggtgcg gcctgtgagg atcagcttat
      901 cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta
      961 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
     1021 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     1081 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     1141 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     1201 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     1261 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     1321 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt
     1381 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     1441 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     1501 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     1561 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     1621 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca
     1681 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     1741 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     1801 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     1861 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     1921 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     1981 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     2041 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     2101 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     2161 tcttcttgag attctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     2221 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     2281 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     2341 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     2401 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     2461 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     2521 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     2581 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     2641 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     2701 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     2761 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttgaagctg tccctgatgg
     2821 tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa
     2881 gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgaactaga tgatccggct
     2941 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     3001 gcaaagcatg cattatgtct agagatccga cgccgccatc tctaggcccg cgccggcccc
     3061 ctcgcacaga cttgtgggag aagctcggct actcccctgc cccggttaat ttgcatataa
     3121 tatttcctag taactataga ggcttaatgt gcgataaaag acagataatc tgttcttttt
     3181 aatactagct acattttaca tgataggctt ggatttctat aagagataca aatactaaat
     3241 tattatttta aaaaacagca caaaaggaaa ctcaccctaa ctgtaaagta attgtgtgtt
     3301 ttgagactat aaatatgcat gcgagaaaag ccttgtttag aagagcgatg ctcttcggtt
     3361 ttagagctag aaatagcaag ttaaaataag gctagtccgt tatcaacttg aaaaagtggc
     3421 accgagtcgg tgcttttttg aattctagat cttgagacaa atggcagtat tcatccacaa
     3481 ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat
     3541 agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg
     3601 ggtttattac agggacagca gagatccact ttggcgccgg ctcgagtggc tccggtgccc
     3661 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
     3721 attgaaccgg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
     3781 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
     3841 acgttctttt tcgcaacggg tttgccgcca gaacacaggt gtcgtgacgc gggatccgcc
     3901 accatgaaac ggacagccga cggaagcgag ttcgagtcac caaagaagaa gcggaaagtc
     3961 agcagtgaaa ccggaccagt ggcagtggac ccaaccctga ggagacggat tgagccccat
     4021 gaatttgaag tgttctttga cccaagggag ctgaggaagg agacatgcct gctgtacgag
     4081 atcaagtggg gcacaagcca caagatctgg cgccacagct ccaagaacac cacaaagcac
     4141 gtggaagtga atttcatcga gaagtttacc tccgagcggc acttctgccc ctctaccagc
     4201 tgttccatca catggtttct gtcttggagc ccttgcggcg agtgttccaa ggccatcacc
     4261 gagttcctgt ctcagcaccc taacgtgacc ctggtcatct acgtggcccg gctgtatcac
     4321 cacatggacc agcagaacag gcagggcctg cgcgatctgg tgaattctgg cgtgaccatc
     4381 cagatcatga cagccccaga gtacgactat tgctggcgga acttcgtgaa ttatccacct
     4441 ggcaaggagg cacactggcc aagataccca cccctgtgga tgaagctgta tgcactggag
     4501 ctgcacgcag gaatcctggg cctgcctcca tgtctgaata tcctgcggag aaagcagccc
     4561 cagctgacat ttttcaccat tgctctgcag tcttgtcact atcagcggct gcctcctcat
     4621 attctgtggg ctacaggcct gaagtctgga ggatctagcg gaggatcctc tggcagcgag
     4681 acaccaggaa caagcgagtc agcaacacca gagagcagtg gcggcagcag cggcggcagc
     4741 gacaagaagt acagcatcgg cctggccatc ggcaccaact ctgtgggctg ggccgtgatc
     4801 accgacgagt acaaggtgcc cagcaagaaa ttcaaggtgc tgggcaacac cgaccggcac
     4861 agcatcaaga agaacctgat cggagccctg ctgttcgaca gcggcgaaac agccgaggcc
     4921 acccggctga agagaaccgc cagaagaaga tacaccagac ggaagaaccg gatctgctat
     4981 ctgcaagaga tcttcagcaa cgagatggcc aaggtggacg acagcttctt ccacagactg
     5041 gaagagtcct tcctggtgga agaggataag aagcacgagc ggcaccccat cttcggcaac
     5101 atcgtggacg aggtggccta ccacgagaag taccccacca tctaccacct gagaaagaaa
     5161 ctggtggaca gcaccgacaa ggccgacctg cggctgatct atctggccct ggcccacatg
     5221 atcaagttcc ggggccactt cctgatcgag ggcgacctga accccgacaa cagcgacgtg
     5281 gacaagctgt tcatccagct ggtgcagacc tacaaccagc tgttcgagga aaaccccatc
     5341 aacgccagcg gcgtggacgc caaggccatc ctgtctgcca gactgagcaa gagcagacgg
     5401 ctggaaaatc tgatcgccca gctgcccggc gagaagaaga atggcctgtt cggaaacctg
     5461 attgccctga gcctgggcct gacccccaac ttcaagagca acttcgacct ggccgaggat
     5521 gccaaactgc agctgagcaa ggacacctac gacgacgacc tggacaacct gctggcccag
     5581 atcggcgacc agtacgccga cctgtttctg gccgccaaga acctgtccga cgccatcctg
     5641 ctgagcgaca tcctgagagt gaacaccgag atcaccaagg cccccctgag cgcctctatg
     5701 atcaagagat acgacgagca ccaccaggac ctgaccctgc tgaaagctct cgtgcggcag
     5761 cagctgcctg agaagtacaa agagattttc ttcgaccaga gcaagaacgg ctacgccggc
     5821 tacattgacg gcggagccag ccaggaagag ttctacaagt tcatcaagcc catcctggaa
     5881 aagatggacg gcaccgagga actgctcgtg aagctgaaca gagaggacct gctgcggaag
     5941 cagcggacct tcgacaacgg cagcatcccc caccagatcc acctgggaga gctgcacgcc
     6001 attctgcggc ggcaggaaga tttttaccca ttcctgaagg acaaccggga aaagatcgag
     6061 aagatcctga ccttccgcat cccctactac gtgggccctc tggccagggg aaacagcaga
     6121 ttcgcctgga tgaccagaaa gagcgaggaa accatcaccc cctggaactt cgaggaagtg
     6181 gtggacaagg gcgcttccgc ccagagcttc atcgagcgga tgaccaactt cgataagaac
     6241 ctgcccaacg agaaggtgct gcccaagcac agcctgctgt acgagtactt caccgtgtat
     6301 aacgagctga ccaaagtgaa atacgtgacc gagggaatga gaaagcccgc cttcctgagc
     6361 ggcgagcaga aaaaggccat cgtggacctg ctgttcaaga ccaaccggaa agtgaccgtg
     6421 aagcagctga aagaggacta cttcaagaaa atcgagtgct tcgactccgt ggaaatctcc
     6481 ggcgtggaag atcggttcaa cgcctccctg ggcacatacc acgatctgct gaaaattatc
     6541 aaggacaagg acttcctgga caatgaggaa aacgaggaca ttctggaaga tatcgtgctg
     6601 accctgacac tgtttgagga cagagagatg atcgaggaac ggctgaaaac ctatgcccac
     6661 ctgttcgacg acaaagtgat gaagcagctg aagcggcgga gatacaccgg ctggggcagg
     6721 ctgagccgga agctgatcaa cggcatccgg gacaagcagt ccggcaagac aatcctggat
     6781 ttcctgaagt ccgacggctt cgccaacaga aacttcatgc agctgatcca cgacgacagc
     6841 ctgaccttta aagaggacat ccagaaagcc caggtgtccg gccagggcga tagcctgcac
     6901 gagcacattg ccaatctggc cggcagcccc gccattaaga agggcatcct gcagacagtg
     6961 aaggtggtgg acgagctcgt gaaagtgatg ggccggcaca agcccgagaa catcgtgatc
     7021 gaaatggcca gagagaacca gaccacccag aagggacaga agaacagccg cgagagaatg
     7081 aagcggatcg aagagggcat caaagagctg ggcagccaga tcctgaaaga acaccccgtg
     7141 gaaaacaccc agctgcagaa cgagaagctg tacctgtact acctgcagaa tgggcgggat
     7201 atgtacgtgg accaggaact ggacatcaac cggctgtccg actacgatgt ggaccatatc
     7261 gtgcctcaga gctttctgaa ggacgactcc atcgacaaca aggtgctgac cagaagcgac
     7321 aagaaccggg gcaagagcga caacgtgccc tccgaagagg tcgtgaagaa gatgaagaac
     7381 tactggcggc agctgctgaa cgccaagctg attacccaga gaaagttcga caatctgacc
     7441 aaggccgaga gaggcggcct gagcgaactg gataaggccg gcttcatcaa gagacagctg
     7501 gtggaaaccc ggcagatcac aaagcacgtg gcacagatcc tggactcccg gatgaacact
     7561 aagtacgacg agaatgacaa gctgatccgg gaagtgaaag tgatcaccct gaagtccaag
     7621 ctggtgtccg atttccggaa ggatttccag ttttacaaag tgcgcgagat caacaactac
     7681 caccacgccc acgacgccta cctaaacgcc gtcgtgggaa ccgccctgat caaaaagtac
     7741 cctaagctgg aaagcgagtt cgtgtacggc gactacaagg tgtacgacgt gcggaagatg
     7801 atcgccaaga gcgagcagga aatcggcaag gctaccgcca agtacttctt ctacagcaac
     7861 atcatgaact ttttcaagac cgagattacc ctggccaacg gcgagatccg gaagcggcct
     7921 ctgatcgaga caaacggcga aaccggggag atcgtgtggg ataagggccg ggattttgcc
     7981 accgtgcgga aagtgctgag catgccccaa gtgaatatcg tgaaaaagac cgaggtgcag
     8041 acaggcggct tcagcaaaga gtctatcagg cccaagagga acagcgataa gctgatcgcc
     8101 agaaagaagg actgggaccc taagaagtac ggcggcttcg tcagccccac cgtggcctat
     8161 tctgtgctgg tggtggccaa agtggaaaag ggcaagtcca agaaactgaa gagtgtgaaa
     8221 gagctgctgg ggatcaccat catggaaaga agcagcttcg agaagaatcc catcgacttt
     8281 ctggaagcca agggctacaa agaagtgaaa aaggacctga tcatcaagct gcctaagtac
     8341 tccctgttcg agctggaaaa cggccggaag agaatgctgg cctctgcccg cttcctgcag
     8401 aagggaaacg aactggccct gccctccaaa tatgtgaact tcctgtacct ggccagccac
     8461 tatgagaagc tgaagggctc ccccgaggat aatgagcaga aacagctgtt tgtggaacag
     8521 cacaagcact acctggacga gatcatcgag cagatcagcg agttctccaa gagagtgatc
     8581 ctggccgacg ctaatctgga caaagtgctg tccgcctaca acaagcaccg ggataagccc
     8641 atcagagagc aggccgagaa tatcatccac ctgtttaccc tgaccaatct gggagcccct
     8701 cgcgccttca agtactttga caccaccatc gaccggaagg tgtacagaag caccaaagag
     8761 gtgctggacg ccaccctgat ccaccagagc atcaccggcc tgtacgagac acggatcgac
     8821 ctgtctcagc tgggaggtga cagcggcggg agcggcggga gcggggggag cactaatctg
     8881 agcgacatca ttgagaagga gactgggaaa cagctggtca ttcaggagtc catcctgatg
     8941 ctgcctgagg aggtggagga agtgatcggc aacaagccag agtctgacat cctggtgcac
     9001 accgcctacg acgagtccac agatgagaat gtgatgctgc tgacctctga cgcccccgag
     9061 tataagcctt gggccctggt catccaggat tctaacggcg agaataagat caagatgctg
     9121 agcggaggat ccggaggatc tggaggcagc accaacctgt ctgacatcat cgagaaggag
     9181 acaggcaagc agctggtcat ccaggagagc atcctgatgc tgcccgaaga agtcgaagaa
     9241 gtgatcggaa acaagcctga gagcgatatc ctggtccata ccgcctacga cgagagtacc
     9301 gacgaaaatg tgatgctgct gacatccgac gccccagagt ataagccctg ggctctggtc
     9361 atccaggatt ccaacggaga gaacaaaatc aaaatgctgt ctggcggctc aaaaagaacc
     9421 gccgacggca gcgaattcga gcccaagaag aagaggaaag tctaaccggt catcatcacc
     9481 atcaccattg agtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat
     9541 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
     9601 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
     9661 ggggtggggt ggggcaggac agcaaggggg aggattggga agagaatagc aggcatgctg
     9721 gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc tcgataccgc
     9781 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
     9841 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
     9901 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
     9961 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
    10021 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
    10081 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttggataa
    10141 tggccaagcc tttgtctcaa gaagaatcca ccctcattga aagagcaacg gctacaatca
    10201 acagcatccc catctctgaa gactacagcg tcgccagcgc agctctctct agcgacggcc
    10261 gcatcttcac tggtgtcaat gtatatcatt ttactggggg accttgtgca gaactcgtgg
    10321 tgctgggcac tgctgctgct gcggcagctg gcaacctgac ttgtatcgtc gcgatcggaa
    10381 atgagaacag gggcatcttg agcccctgcg gacggtgccg acaggtgctt ctcgatctgc
    10441 atcctgggat caaagccata gtgaaggaca gtgatggaca gccgacggca gttgggattc
    10501 gtgaattgct gccctctggt tatgtgtggg agggctaaga attcttcgaa atgaccgacc
    10561 aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt
    10621 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca
    10681 tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa
    10741 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
    10801 tgtccaaact catcaatgta tcttatcatg tctggatccc ttgaaataca tccacaggta
    10861 cacctccaat tgactcaaat gatgtcaatt agtctatcag aagcttgtgg aaggctggag
    10921 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
    10981 gcgggtgttg gcgggtgtcg gggcgcagcc atgaggtcga ctctagagga tcgatccccg
    11041 ccccggacga actaaacctg actacgacat ctctgcccct tcttcgcggg gcagtgcatg
    11101 taatcccttc agttggttgg tacaacttgc caactgggcc ctgttccaca tgtgacacgg
    11161 ggggggacca aacacaaagg ggttctctga ctgtagttga catccttata aatggatgtg
    11221 cacatttgcc aacactgagt ggctttcatc ctggagcaga ctttgcagtc tgtggactgc
    11281 aacacaacat tgcctttatg tgtaactctt ggctgaagct cttacaccaa tgctggggga
    11341 catgtacctc ccaggggccc aggaagacta cgggaggcta caccaacgtc aatcagaggg
    11401 gcctgtgtag ctaccgataa gcggaccctc aagagggcat tagcaatagt gtttataagg
    11461 cccccttgtt aaccctaaac gggtagcata tgcttcccgg gtagtagtat atactatcca
    11521 gactaaccct aattcaatag catatgttac ccaacgggaa gcatatgcta tcgaattagg
    11581 gttagtaaaa gggtcctaag gaacagcgat atctcccacc ccatgagctg tcacggtttt
    11641 atttacatgg ggtcaggatt ccacgagggt agtgaaccat tttagtcaca agggcagtgg
    11701 ctgaagatca aggagcgggc agtgaactct cctgaatctt cgcctgcttc ttcattctcc
    11761 ttcgtttagc taatagaata actgctgagt tgtgaacagt aaggtgtatg tgaggtgctc
    11821 gaaaacaagg tttcaggtga cgcccccaga ataaaatttg gacggggggt tcagtggtgg
    11881 cattgtgcta tgacaccaat ataaccctca caaacccctt gggcaataaa tactagtgta
    11941 ggaatgaaac attctgaata tctttaacaa tagaaatcca tggggtgggg acaagccgta
    12001 aagactggat gtccatctca cacgaattta tggctatggg caacacataa tcctagtgca
    12061 atatgatact ggggttatta agatgtgtcc caggcaggga ccaagacagg tgaaccatgt
    12121 tgttacactc tatttgtaac aaggggaaag agagtggacg ccgacagcag cggactccac
    12181 tggttgtctc taacaccccc gaaaattaaa cggggctcca cgccaatggg gcccataaac
    12241 aaagacaagt ggccactctt ttttttgaaa ttgtggagtg ggggcacgcg tcagccccca
    12301 cacgccgccc tgcggttttg gactgtaaaa taagggtgta ataacttggc tgattgtaac
    12361 cccgctaacc actgcggtca aaccacttgc ccacaaaacc actaatggca ccccggggaa
    12421 tacctgcata agtaggtggg cgggccaaga taggggcgcg attgctgcga tctggaggac
    12481 aaattacaca cacttgcgcc tgagcgccaa gcacagggtt gttggtcctc atattcacga
    12541 ggtcgctgag agcacggtgg gctaatgttg ccatgggtag catatactac ccaaatatct
    12601 ggatagcata tgctatccta atctatatct gggtagcata ggctatccta atctatatct
    12661 gggtagtata tgctatccta atctgtatcc gggtagcata tgctatccta atagagatta
    12721 gggtagcata tgctatccta atctatatct gggtagcata ggctatccta atctatatct
    12781 gggtagtata tgctatccta atctgtatcc gggtagcata tgctatccta atagagatta
    12841 gggtagtata tgctatccta atttatatct gggtagcata tactacccaa atatctggat
    12901 agcatatgct atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt
    12961 agcataggct atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt
    13021 agtatatgct atcctaattt atatctgggt agcataggct atcctaatct atatctgggt
    13081 agcatatgct atcctaatct atatctgggt agtatatgct atcctaatct gtatccgggt
    13141 agcatatgct atcctcatgc atatacagtc agcatatgat acccagtagt agagtgggag
    13201 tgctatcctt tgcatatgcc gccacctccc aagggggcgt gaattttcgc tgcttgtcct
    13261 tttcctgctg gttgctccca ttcttaggtg aatttaagga ggccaggcta aagccgtcgc
    13321 atgtctgatt gctcaccagg taaatgtcgc taatgttttc caacgcgaga aggtgttgag
    13381 cgcggagctg agtgacgtga caacatgggt atgcccaatt gccccatgtt gggaggacga
    13441 aaatggtgac aagacagatg gccagaaata caccaacagc acgcatgatg tctactgggg
    13501 atttattctt tagtgcgggg gaatacacgg cttttaatac gattgagggc gtctcctaac
    13561 aagttacatc actcctgccc ttcctcaccc tcatctccat cacctccttc atctccgtca
    13621 tctccgtcat caccctccgc ggcagcccct tccaccatag gtggaaacca gggaggcaaa
    13681 tctactccat cgtcaaagct gcacacagtc accctgatat tgcaggtagg agcgggcttt
    13741 gtcataacaa ggtccttaat cgcatccttc aaaacctcag caaatatatg agtttgtaaa
    13801 aagaccatga aataacagac aatggactcc cttagcgggc caggttgtgg gccgggtcca
    13861 ggggccattc caaaggggag acgactcaat ggtgtaagac gacattgtgg aatagcaagg
    13921 gcagttcctc gccttaggtt gtaaagggag gtcttactac ctccatatac gaacacaccg
    13981 gcgacccaag ttccttcgtc ggtagtcctt tctacgtgac tcctagccag gagagctctt
    14041 aaaccttctg caatgttctc aaatttcggg ttggaacctc cttgaccacg atgctttcca
    14101 aaccaccctc cttttttgcg cctgcctcca tcaccctgac cccggggtcc agtgcttggg
    14161 ccttctcctg ggtcatctgc ggggccctgc tctatcgctc ccgggggcac gtcaggctca
    14221 ccatctgggc caccttcttg gtggtattca aaataatcgg cttcccctac agggtggaaa
    14281 aatggccttc tacctggagg gggcctgcgc ggtggagacc cggatgatga tgactgacta
    14341 ctgggactcc tgggcctctt ttctccacgt ccacgacctc tccccctggc tctttcacga
    14401 cttccccccc tggctctttc acgtcctcta ccccggcggc ctccactacc tcctcgaccc
    14461 cggcctccac tacctcctcg accccggcct ccactgcctc ctcgaccccg gcctccacct
    14521 cctgctcctg cccctcctgc tcctgcccct cctcctgctc ctgcccctcc tgctcctgcc
    14581 cctcccgctc ctgctcctgc tcctgttcca ccgtgggtcc ctttgcagcc aatgcaactt
    14641 ggacgttttt ggggtctccg gacaccatct ctatgt
//
