LOCUS       sequence-292679-       14290 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses ABEmax(with SpCas9-NG), EBNA1 and blasticidin resistence 
            gene; cloning backbone for sgRNA; Contains Epstein-Barr virus oriP 
            replication origin .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 14290)
  AUTHORS   Qi T, Wu F, Xie Y, Gao S, Li M, Pu J, Li D, Lan F, Wang Y
  TITLE     Base Editing Mediated Generation of Point Mutations Into Human 
            Pluripotent Stem Cells for Modeling Disease.
  JOURNAL   Front Cell Dev Biol. 2020 Sep 25;8:590581. doi: 
            10.3389/fcell.2020.590581. eCollection 2020.
  PUBMED    33102492
REFERENCE   2  (bases 1 to 14290)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Cell 
            Dev Biol."; date: "2020-09-25"; volume: "8:590581. doi"; pages: " 
            10.3389/fcell.2020.590581. eCollection 2020"
COMMENT     Sequence Label: epi-ABEmax-NG
FEATURES             Location/Qualifiers
     source          1..14290
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(892..910)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        978..1082
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1083..1151,1152..1943)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1301..1320)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2114..2702
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2603..2622
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2972..3285
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for mouse U6 snRNA (Das 
                     et al., 1988)"
     primer_bind     3204..3224
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     misc_RNA        3306..3381
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    3429..3546
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3615..3826
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             3888..3908
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5097..9197
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SIRPKRNSDKLIARKKDWDPKKYGGFVSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASARFLQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPRAFKYFDTTIDRKVYRSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             9240..9260
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(9265..9282)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9271..9495
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        9543..9872
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9723..9858
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9894..10292
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    10458..10592
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10495..10514)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10549..10568
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      11232..13021
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
ORIGIN
        1 ctctttcacg tcctctaccc cggcggcctc cactacctcc tcgaccccgg cctccactac
       61 ctcctcgacc ccggcctcca ctgcctcctc gaccccggcc tccacctcct gctcctgccc
      121 ctcctgctcc tgcccctcct cctgctcctg cccctcctgc tcctgcccct cccgctcctg
      181 ctcctgctcc tgttccaccg tgggtccctt tgcagccaat gcaacttgga cgtttttggg
      241 gtctccggac accatctcta tgtcttggcc ctgatcctga gccgcccggg gctcctggtc
      301 ttccgcctcc tcgtcctcgt cctcttcccc gtcctcgtcc atggttatca ccccctcttc
      361 tttgaggtcc actgccgccg gagccttctg gtccagatgt gtctcccttc tctcctaggc
      421 catttccagg tcctgtacct ggcccctcgt cagacatgat tcacactaaa agagatcaat
      481 agacatcttt attagacgac gctcagtgaa tacagggagt gcagactcct gccccctcca
      541 acagcccccc caccctcatc cccttcatgg tcgctgtcag acagatccag gtctgaaaat
      601 tccccatcct ccgaaccatc ctcgtcctca tcaccaatta ctcgcagccc ggaaaactcc
      661 cgctgaacat cctcaagatt tgcgtcctga gcctcaagcc aggcctcaaa ttcctcgtcc
      721 ccctttttgc tggacggtag ggatggggat tctcgggacc cctcctcttc ctcttcaagg
      781 tcaccagaca gagatgctac tggggcaacg gaagaaaagc tgggtgcggc ctgtgaggat
      841 cagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga
      901 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
      961 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     1021 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     1081 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     1141 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     1201 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     1261 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     1321 cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     1381 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     1441 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     1501 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     1561 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     1621 tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     1681 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     1741 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     1801 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     1861 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     1921 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     1981 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     2041 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     2101 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     2161 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     2221 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
     2281 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     2341 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     2401 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     2461 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     2521 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     2581 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     2641 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     2701 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tgaagctgtc
     2761 cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc
     2821 cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gaactagatg
     2881 atccggctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     2941 agaagtatgc aaagcatgca ttatgtctag agatccgacg ccgccatctc taggcccgcg
     3001 ccggccccct cgcacagact tgtgggagaa gctcggctac tcccctgccc cggttaattt
     3061 gcatataata tttcctagta actatagagg cttaatgtgc gataaaagac agataatctg
     3121 ttctttttaa tactagctac attttacatg ataggcttgg atttctataa gagatacaaa
     3181 tactaaatta ttattttaaa aaacagcaca aaaggaaact caccctaact gtaaagtaat
     3241 tgtgtgtttt gagactataa atatgcatgc gagaaaagcc ttgtttagaa gagcgatgct
     3301 cttcggtttt agagctagaa atagcaagtt aaaataaggc tagtccgtta tcaacttgaa
     3361 aaagtggcac cgagtcggtg cttttttgaa ttctagatct tgagacaaat ggcagtattc
     3421 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     3481 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     3541 aattttcggg tttattacag ggacagcaga gatccacttt ggcgccggct cgagtggctc
     3601 cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg
     3661 ggtcggcaat tgaaccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt
     3721 cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt
     3781 cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacaggtgt cgtgacgcgg
     3841 gatccgccac catgaaacgg acagccgacg gaagcgagtt cgagtcacca aagaagaagc
     3901 ggaaagtctc tgaagtcgag tttagccacg agtattggat gaggcacgca ctgaccctgg
     3961 caaagcgagc atgggatgaa agagaagtcc ccgtgggcgc cgtgctggtg cacaacaata
     4021 gagtaatcgg agagggatgg aacaggccaa tcggccgcca cgaccctacc gcacacgcag
     4081 agatcatggc actgaggcag ggaggcctgg tcatgcagaa ttaccgcctg atcgatgcca
     4141 ccctgtatgt gacactggag ccatgcgtga tgtgcgcagg agcaatgatc cacagcagga
     4201 tcggaagagt ggtgttcgga gcacgggacg ccaagaccgg cgcagcaggc tccctgatgg
     4261 atgtgctgca ccaccccggc atgaaccacc gggtggagat cacagaggga atcctggcag
     4321 acgagtgcgc cgccctgctg agcgatttct ttagaatgcg gagacaggag atcaaggccc
     4381 agaagaaggc acagagctcc accgactctg gaggatctag cggaggatcc tctggaagcg
     4441 agacaccagg cacaagcgag tccgccacac cagagagctc cggcggctcc tccggaggat
     4501 cctctgaggt ggagttttcc cacgagtact ggatgagaca tgccctgacc ctggccaaga
     4561 gggcacgcga tgagagggag gtgcctgtgg gagccgtgct ggtgctgaac aatagagtga
     4621 tcggcgaggg ctggaacaga gccatcggcc tgcacgaccc aacagcccat gccgaaatta
     4681 tggccctgag acagggcggc ctggtcatgc agaactacag actgattgac gccaccctgt
     4741 acgtgacatt cgagccttgc gtgatgtgcg ccggcgccat gatccactct aggatcggcc
     4801 gcgtggtgtt tggcgtgagg aacgcaaaaa ccggcgccgc aggctccctg atggacgtgc
     4861 tgcactaccc cggcatgaat caccgcgtcg aaattaccga gggaatcctg gcagatgaat
     4921 gtgccgccct gctgtgctat ttctttcgga tgcctagaca ggtgttcaat gctcagaaga
     4981 aggcccagag ctccaccgac tccggaggat ctagcggagg ctcctctggc tctgagacac
     5041 ctggcacaag cgagagcgca acacctgaaa gcagcggggg cagcagcggg gggtcagaca
     5101 agaagtacag catcggcctg gccatcggca ccaactctgt gggctgggcc gtgatcaccg
     5161 acgagtacaa ggtgcccagc aagaaattca aggtgctggg caacaccgac cggcacagca
     5221 tcaagaagaa cctgatcgga gccctgctgt tcgacagcgg cgaaacagcc gaggccaccc
     5281 ggctgaagag aaccgccaga agaagataca ccagacggaa gaaccggatc tgctatctgc
     5341 aagagatctt cagcaacgag atggccaagg tggacgacag cttcttccac agactggaag
     5401 agtccttcct ggtggaagag gataagaagc acgagcggca ccccatcttc ggcaacatcg
     5461 tggacgaggt ggcctaccac gagaagtacc ccaccatcta ccacctgaga aagaaactgg
     5521 tggacagcac cgacaaggcc gacctgcggc tgatctatct ggccctggcc cacatgatca
     5581 agttccgggg ccacttcctg atcgagggcg acctgaaccc cgacaacagc gacgtggaca
     5641 agctgttcat ccagctggtg cagacctaca accagctgtt cgaggaaaac cccatcaacg
     5701 ccagcggcgt ggacgccaag gccatcctgt ctgccagact gagcaagagc agacggctgg
     5761 aaaatctgat cgcccagctg cccggcgaga agaagaatgg cctgttcgga aacctgattg
     5821 ccctgagcct gggcctgacc cccaacttca agagcaactt cgacctggcc gaggatgcca
     5881 aactgcagct gagcaaggac acctacgacg acgacctgga caacctgctg gcccagatcg
     5941 gcgaccagta cgccgacctg tttctggccg ccaagaacct gtccgacgcc atcctgctga
     6001 gcgacatcct gagagtgaac accgagatca ccaaggcccc cctgagcgcc tctatgatca
     6061 agagatacga cgagcaccac caggacctga ccctgctgaa agctctcgtg cggcagcagc
     6121 tgcctgagaa gtacaaagag attttcttcg accagagcaa gaacggctac gccggctaca
     6181 ttgacggcgg agccagccag gaagagttct acaagttcat caagcccatc ctggaaaaga
     6241 tggacggcac cgaggaactg ctcgtgaagc tgaacagaga ggacctgctg cggaagcagc
     6301 ggaccttcga caacggcagc atcccccacc agatccacct gggagagctg cacgccattc
     6361 tgcggcggca ggaagatttt tacccattcc tgaaggacaa ccgggaaaag atcgagaaga
     6421 tcctgacctt ccgcatcccc tactacgtgg gccctctggc caggggaaac agcagattcg
     6481 cctggatgac cagaaagagc gaggaaacca tcaccccctg gaacttcgag gaagtggtgg
     6541 acaagggcgc ttccgcccag agcttcatcg agcggatgac caacttcgat aagaacctgc
     6601 ccaacgagaa ggtgctgccc aagcacagcc tgctgtacga gtacttcacc gtgtataacg
     6661 agctgaccaa agtgaaatac gtgaccgagg gaatgagaaa gcccgccttc ctgagcggcg
     6721 agcagaaaaa ggccatcgtg gacctgctgt tcaagaccaa ccggaaagtg accgtgaagc
     6781 agctgaaaga ggactacttc aagaaaatcg agtgcttcga ctccgtggaa atctccggcg
     6841 tggaagatcg gttcaacgcc tccctgggca cataccacga tctgctgaaa attatcaagg
     6901 acaaggactt cctggacaat gaggaaaacg aggacattct ggaagatatc gtgctgaccc
     6961 tgacactgtt tgaggacaga gagatgatcg aggaacggct gaaaacctat gcccacctgt
     7021 tcgacgacaa agtgatgaag cagctgaagc ggcggagata caccggctgg ggcaggctga
     7081 gccggaagct gatcaacggc atccgggaca agcagtccgg caagacaatc ctggatttcc
     7141 tgaagtccga cggcttcgcc aacagaaact tcatgcagct gatccacgac gacagcctga
     7201 cctttaaaga ggacatccag aaagcccagg tgtccggcca gggcgatagc ctgcacgagc
     7261 acattgccaa tctggccggc agccccgcca ttaagaaggg catcctgcag acagtgaagg
     7321 tggtggacga gctcgtgaaa gtgatgggcc ggcacaagcc cgagaacatc gtgatcgaaa
     7381 tggccagaga gaaccagacc acccagaagg gacagaagaa cagccgcgag agaatgaagc
     7441 ggatcgaaga gggcatcaaa gagctgggca gccagatcct gaaagaacac cccgtggaaa
     7501 acacccagct gcagaacgag aagctgtacc tgtactacct gcagaatggg cgggatatgt
     7561 acgtggacca ggaactggac atcaaccggc tgtccgacta cgatgtggac catatcgtgc
     7621 ctcagagctt tctgaaggac gactccatcg acaacaaggt gctgaccaga agcgacaaga
     7681 accggggcaa gagcgacaac gtgccctccg aagaggtcgt gaagaagatg aagaactact
     7741 ggcggcagct gctgaacgcc aagctgatta cccagagaaa gttcgacaat ctgaccaagg
     7801 ccgagagagg cggcctgagc gaactggata aggccggctt catcaagaga cagctggtgg
     7861 aaacccggca gatcacaaag cacgtggcac agatcctgga ctcccggatg aacactaagt
     7921 acgacgagaa tgacaagctg atccgggaag tgaaagtgat caccctgaag tccaagctgg
     7981 tgtccgattt ccggaaggat ttccagtttt acaaagtgcg cgagatcaac aactaccacc
     8041 acgcccacga cgcctacctg aacgccgtcg tgggaaccgc cctgatcaaa aagtacccta
     8101 agctggaaag cgagttcgtg tacggcgact acaaggtgta cgacgtgcgg aagatgatcg
     8161 ccaagagcga gcaggaaatc ggcaaggcta ccgccaagta cttcttctac agcaacatca
     8221 tgaacttttt caagaccgag attaccctgg ccaacggcga gatccggaag cggcctctga
     8281 tcgagacaaa cggcgaaacc ggggagatcg tgtgggataa gggccgggat tttgccaccg
     8341 tgcggaaagt gctgagcatg ccccaagtga atatcgtgaa aaagaccgag gtgcagacag
     8401 gcggcttcag caaagagtct atcaggccca agaggaacag cgataagctg atcgccagaa
     8461 agaaggactg ggaccctaag aagtacggcg gcttcgtcag ccccaccgtg gcctattctg
     8521 tgctggtggt ggccaaagtg gaaaagggca agtccaagaa actgaagagt gtgaaagagc
     8581 tgctggggat caccatcatg gaaagaagca gcttcgagaa gaatcccatc gactttctgg
     8641 aagccaaggg ctacaaagaa gtgaaaaagg acctgatcat caagctgcct aagtactccc
     8701 tgttcgagct ggaaaacggc cggaagagaa tgctggcctc tgcccgcttc ctgcagaagg
     8761 gaaacgaact ggccctgccc tccaaatatg tgaacttcct gtacctggcc agccactatg
     8821 agaagctgaa gggctccccc gaggataatg agcagaaaca gctgtttgtg gaacagcaca
     8881 agcactacct ggacgagatc atcgagcaga tcagcgagtt ctccaagaga gtgatcctgg
     8941 ccgacgctaa tctggacaaa gtgctgtccg cctacaacaa gcaccgggat aagcccatca
     9001 gagagcaggc cgagaatatc atccacctgt ttaccctgac caatctggga gcccctcgcg
     9061 ccttcaagta ctttgacacc accatcgacc ggaaggtgta cagaagcacc aaagaggtgc
     9121 tggacgccac cctgatccac cagagcatca ccggcctgta cgagacacgg atcgacctgt
     9181 ctcagctggg aggtgactct ggcggctcaa aaagaaccgc cgacggcagc gaattcgagc
     9241 ccaagaagaa gaggaaagtc taagcctcga ctgtgccttc tagttgccag ccatctgttg
     9301 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
     9361 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
     9421 gggtggggca ggacagcaag ggggaggatt gggaagagaa tagcaggcat gctggggatg
     9481 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctcgata ccgctgtgga
     9541 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     9601 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
     9661 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     9721 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     9781 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     9841 gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgg ataatggcca
     9901 agcctttgtc tcaagaagaa tccaccctca ttgaaagagc aacggctaca atcaacagca
     9961 tccccatctc tgaagactac agcgtcgcca gcgcagctct ctctagcgac ggccgcatct
    10021 tcactggtgt caatgtatat cattttactg ggggaccttg tgcagaactc gtggtgctgg
    10081 gcactgctgc tgctgcggca gctggcaacc tgacttgtat cgtcgcgatc ggaaatgaga
    10141 acaggggcat cttgagcccc tgcggacggt gccgacaggt gcttctcgat ctgcatcctg
    10201 ggatcaaagc catagtgaag gacagtgatg gacagccgac ggcagttggg attcgtgaat
    10261 tgctgccctc tggttatgtg tgggagggct aagaattctt cgaaatgacc gaccaagcga
    10321 cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct
    10381 tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg
    10441 agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata
    10501 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
    10561 aactcatcaa tgtatcttat catgtctgga tcccttgaaa tacatccaca ggtacacctc
    10621 caattgactc aaatgatgtc aattagtcta tcagaagctt gtggaaggct ggagacggtc
    10681 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
    10741 gttggcgggt gtcggggcgc agccatgagg tcgactctag aggatcgatc cccgccccgg
    10801 acgaactaaa cctgactacg acatctctgc cccttcttcg cggggcagtg catgtaatcc
    10861 cttcagttgg ttggtacaac ttgccaactg ggccctgttc cacatgtgac acgggggggg
    10921 accaaacaca aaggggttct ctgactgtag ttgacatcct tataaatgga tgtgcacatt
    10981 tgccaacact gagtggcttt catcctggag cagactttgc agtctgtgga ctgcaacaca
    11041 acattgcctt tatgtgtaac tcttggctga agctcttaca ccaatgctgg gggacatgta
    11101 cctcccaggg gcccaggaag actacgggag gctacaccaa cgtcaatcag aggggcctgt
    11161 gtagctaccg ataagcggac cctcaagagg gcattagcaa tagtgtttat aaggccccct
    11221 tgttaaccct aaacgggtag catatgcttc ccgggtagta gtatatacta tccagactaa
    11281 ccctaattca atagcatatg ttacccaacg ggaagcatat gctatcgaat tagggttagt
    11341 aaaagggtcc taaggaacag cgatatctcc caccccatga gctgtcacgg ttttatttac
    11401 atggggtcag gattccacga gggtagtgaa ccattttagt cacaagggca gtggctgaag
    11461 atcaaggagc gggcagtgaa ctctcctgaa tcttcgcctg cttcttcatt ctccttcgtt
    11521 tagctaatag aataactgct gagttgtgaa cagtaaggtg tatgtgaggt gctcgaaaac
    11581 aaggtttcag gtgacgcccc cagaataaaa tttggacggg gggttcagtg gtggcattgt
    11641 gctatgacac caatataacc ctcacaaacc ccttgggcaa taaatactag tgtaggaatg
    11701 aaacattctg aatatcttta acaatagaaa tccatggggt ggggacaagc cgtaaagact
    11761 ggatgtccat ctcacacgaa tttatggcta tgggcaacac ataatcctag tgcaatatga
    11821 tactggggtt attaagatgt gtcccaggca gggaccaaga caggtgaacc atgttgttac
    11881 actctatttg taacaagggg aaagagagtg gacgccgaca gcagcggact ccactggttg
    11941 tctctaacac ccccgaaaat taaacggggc tccacgccaa tggggcccat aaacaaagac
    12001 aagtggccac tctttttttt gaaattgtgg agtgggggca cgcgtcagcc cccacacgcc
    12061 gccctgcggt tttggactgt aaaataaggg tgtaataact tggctgattg taaccccgct
    12121 aaccactgcg gtcaaaccac ttgcccacaa aaccactaat ggcaccccgg ggaatacctg
    12181 cataagtagg tgggcgggcc aagatagggg cgcgattgct gcgatctgga ggacaaatta
    12241 cacacacttg cgcctgagcg ccaagcacag ggttgttggt cctcatattc acgaggtcgc
    12301 tgagagcacg gtgggctaat gttgccatgg gtagcatata ctacccaaat atctggatag
    12361 catatgctat cctaatctat atctgggtag cataggctat cctaatctat atctgggtag
    12421 tatatgctat cctaatctgt atccgggtag catatgctat cctaatagag attagggtag
    12481 catatgctat cctaatctat atctgggtag cataggctat cctaatctat atctgggtag
    12541 tatatgctat cctaatctgt atccgggtag catatgctat cctaatagag attagggtag
    12601 tatatgctat cctaatttat atctgggtag catatactac ccaaatatct ggatagcata
    12661 tgctatccta atctatatct gggtagcata tgctatccta atctatatct gggtagcata
    12721 ggctatccta atctatatct gggtagcata tgctatccta atctatatct gggtagtata
    12781 tgctatccta atttatatct gggtagcata ggctatccta atctatatct gggtagcata
    12841 tgctatccta atctatatct gggtagtata tgctatccta atctgtatcc gggtagcata
    12901 tgctatcctc atgcatatac agtcagcata tgatacccag tagtagagtg ggagtgctat
    12961 cctttgcata tgccgccacc tcccaagggg gcgtgaattt tcgctgcttg tccttttcct
    13021 gctggttgct cccattctta ggtgaattta aggaggccag gctaaagccg tcgcatgtct
    13081 gattgctcac caggtaaatg tcgctaatgt tttccaacgc gagaaggtgt tgagcgcgga
    13141 gctgagtgac gtgacaacat gggtatgccc aattgcccca tgttgggagg acgaaaatgg
    13201 tgacaagaca gatggccaga aatacaccaa cagcacgcat gatgtctact ggggatttat
    13261 tctttagtgc gggggaatac acggctttta atacgattga gggcgtctcc taacaagtta
    13321 catcactcct gcccttcctc accctcatct ccatcacctc cttcatctcc gtcatctccg
    13381 tcatcaccct ccgcggcagc cccttccacc ataggtggaa accagggagg caaatctact
    13441 ccatcgtcaa agctgcacac agtcaccctg atattgcagg taggagcggg ctttgtcata
    13501 acaaggtcct taatcgcatc cttcaaaacc tcagcaaata tatgagtttg taaaaagacc
    13561 atgaaataac agacaatgga ctcccttagc gggccaggtt gtgggccggg tccaggggcc
    13621 attccaaagg ggagacgact caatggtgta agacgacatt gtggaatagc aagggcagtt
    13681 cctcgcctta ggttgtaaag ggaggtctta ctacctccat atacgaacac accggcgacc
    13741 caagttcctt cgtcggtagt cctttctacg tgactcctag ccaggagagc tcttaaacct
    13801 tctgcaatgt tctcaaattt cgggttggaa cctccttgac cacgatgctt tccaaaccac
    13861 cctccttttt tgcgcctgcc tccatcaccc tgaccccggg gtccagtgct tgggccttct
    13921 cctgggtcat ctgcggggcc ctgctctatc gctcccgggg gcacgtcagg ctcaccatct
    13981 gggccacctt cttggtggta ttcaaaataa tcggcttccc ctacagggtg gaaaaatggc
    14041 cttctacctg gagggggcct gcgcggtgga gacccggatg atgatgactg actactggga
    14101 ctcctgggcc tcttttctcc acgtccacga cctctccccc tggctctttc acgacttccc
    14161 cccctggctc tttcacgtcc tctaccccgg cggcctccac tacctcctcg accccggcct
    14221 ccactacctc ctcgaccccg gcctccactg cctcctcgac cccggcctcc acctcctgct
    14281 cctgcccctc
//
