LOCUS       sequence-292688-        5854 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5854)
  AUTHORS   Pfleger CM, Lee E, Kirschner MW
  TITLE     Substrate recognition by the Cdc20 and Cdh1 components of the 
            anaphase-promoting complex.
  JOURNAL   Genes Dev. 2001 Sep 15. 15(18):2396-407.
  PUBMED    11562349
REFERENCE   2  (bases 1 to 5854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2001 Sep 15. 15(18):2396-407."
COMMENT     Sequence Label: MT-cdc20
FEATURES             Location/Qualifiers
     source          1..5854
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        17..35
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     17..34
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             85..114
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             124..153
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             163..192
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             202..231
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             241..270
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             304..333
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    1888..2022
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1912..1931)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1966..1985
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2135..2155)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2135..2153)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2174..2190)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2174..2190)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2187..2209)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2198..2214
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2222..2228,2229..2246,2247..2252))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2267..2288
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2405..2422)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2576..3164)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2656..2675)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3335..4126,4127..4195))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3958..3977
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4196..4300)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4326..4781)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4463..4484)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4675..4694
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4852..5836
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
ORIGIN
        1 cgtcggagca agcttgattt aggtgacact atagaataca agctacttgt tctttttgca
       61 ggatcccatc gatttaaagc tatggagcaa aagctcattt ctgaagagga cttgaatgaa
      121 atggagcaaa agctcatttc tgaagaggac ttgaatgaaa tggagcaaaa gctcatttct
      181 gaagaggact tgaatgaaat ggagcaaaag ctcatttctg aagaggactt gaatgaaatg
      241 gagcaaaagc tcatttctga agaggacttg aatgaaatgg agagcttggg cgacctcacc
      301 atggagcaaa agctcatttc tgaagaggac ttgaattcga tggcacagtt cgcgttcgag
      361 agtgacctgc actcgctgct tcagctggat gcacccatcc ccaatgcacc ccctgcgcgc
      421 tggcagcgca aagccaagga agccgcaggc ccggccccct cacccatgcg ggccgccaac
      481 cgatcccaca gcgccggcag gactccgggc cgaactcctg gcaaatccag ttccaaggtt
      541 cagaccactc ctagcaaacc tggcggtgac cgctatatcc cccatcgcag tgctgcccag
      601 atggaggtgg ccagcttcct cctgagcaag gagaaccagc ctgaaaacag ccagacgccc
      661 accaagaagg aacatcagaa agcctgggct ttgaacctga acggttttga tgtagaggaa
      721 gccaagatcc ttcggctcag tggaaaacca caaaatgcgc cagagggtta ccagaacaga
      781 ctgaaagtac tctacagcca aaaggccact cctggctcca gccggaagac ctgccgttac
      841 attccttccc tgccagaccg tatcctggat gcgcctgaaa tccgaaatga ctattacctg
      901 aaccttgtgg attggagttc tgggaatgta ctggccgtgg cactggacaa cagtgtgtac
      961 ctgtggagtg caagctctgg tgacatcctg cagcttttgc aaatggagca gcctggggaa
     1021 tatatatcct ctgtggcctg gatcaaagag ggcaactact tggctgtggg caccagcagt
     1081 gctgaggtgc agctatggga tgtgcagcag cagaagcggc ttcgaaatat gaccagtcac
     1141 tctgcccgag tgggctccct aagctggaac agctatatcc tgtccagtgg ttcacgttct
     1201 ggccacatcc accaccatga tgttcgggta gcagaacacc atgtggccac actgagtggc
     1261 cacagccagg aagtgtgtgg gctgcgctgg gccccagatg gacgacattt ggccagtggt
     1321 ggtaatgata acctggtcaa tgtgtggcct agtgctcctg gagagggtgg ctgggttcct
     1381 ctgcagacat tcacccagca tcaaggggct gtcaaggccg tagcatggtg tccctggcag
     1441 tccaatgtcc tggcaacagg agggggcacc agtgatcgac acattcgcat ctggaatgtg
     1501 tgctctgggg cctgtctgag tgccgtggat gcccattccc aggtgtgctc catcctctgg
     1561 tctccccatt acaaggagct catctcaggc catggctttg cacagaacca gctagttatt
     1621 tggaagtacc caaccatggc caaggtggct gaactcaaag gtcacacatc ccgggtcctg
     1681 agtctgacca tgagcccaga tggggccaca gtggcatccg cagcagcaga tgagaccctg
     1741 aggctatggc gctgttttga gttggaccct gcgcggcggc gggagcggga gaaggccagt
     1801 gcagccaaaa gcagcctcat ccaccaaggc atccgctgat ctagagccct ctcgagcctc
     1861 tagaactata gtgagtcgta ttacgtagat ccagacatga taagatacat tgatgagttt
     1921 ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct
     1981 attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt
     2041 cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaattcgc ggccgcggcg
     2101 ccaatgcatt gggcccggta cccagctttt gttcccttta gtgagggtta attgcgcgct
     2161 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     2221 acaacatacg agccgggagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     2281 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     2341 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     2401 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     2461 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     2521 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     2581 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     2641 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     2701 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     2761 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     2821 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     2881 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     2941 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     3001 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     3061 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     3121 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     3181 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     3241 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     3301 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     3361 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     3421 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     3481 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     3541 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     3601 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     3661 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     3721 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     3781 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     3841 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     3901 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     3961 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     4021 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     4081 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     4141 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     4201 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     4261 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     4321 cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
     4381 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
     4441 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
     4501 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
     4561 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
     4621 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
     4681 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
     4741 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct
     4801 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agtcgaccgc
     4861 caattcaata tggcgtatat ggactcatgc caattcaata tggtggatct ggacctgtgc
     4921 caattcaata tggcgtatat ggactcgtgc caattcaata tggtggatct ggaccccagc
     4981 caattcaata tggcggactt ggcaccatgc caattcaata tggcggacct ggcactgtgc
     5041 caactgggga ggggtctact tggcacggtg ccaagtttga ggaggggtct tggccctgtg
     5101 ccaagtccgc catattgaat tggcatggtg ccaataatgg cggccatatt ggctatatgc
     5161 caggatcaat atataggcaa tatccaatat ggccctatgc caatatggct attggccagg
     5221 ttcaatacta tgtattggcc ctatgccata tagtattcca tatatgggtt ttcctattga
     5281 cgtagatagc ccctcccaat gggcggtccc atataccata tatggggctt cctaataccg
     5341 cccatagcca ctcccccatt gacgtcaatg gtctctatat atggtctttc ctattgacgt
     5401 catatgggcg gtcctattga cgtatatggc gcctccccca ttgacgtcaa ttacggtaaa
     5461 tggcccgcct ggctcaatgc ccattgacgt caataggacc acccaccatt gacgtcaatg
     5521 ggatggctca ttgcccattc atatccgttc tcacgccccc tattgacgtc aatgacggta
     5581 aatggcccac ttggcagtac atcaatatct attaatagta acttggcaag tacattacta
     5641 ttggaagtac gccagggtac attggcagta ctcccattga cgtcaatggc ggtaaatggc
     5701 ccgcgatggc tgccaagtac atccccattg acgtcaatgg ggaggggcaa tgacgcaaat
     5761 gggcgttcca ttgacgtaaa tgggcggtag gcgtgcctaa tgggaggtct atataagcaa
     5821 tgctcgttta gggaaccgcc attctgcctg ggga
//
