LOCUS       sequence-296397-         896 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Backbone for cloning LwCas13a guides under U6 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Abudayyeh OO, Gootenberg JS, Essletzbichler P, Han S, Joung J, 
            Belanto JJ, Verdine V, Cox DBT, Kellner MJ, Regev A, Lander ES, 
            Voytas DF, Ting AY, Zhang F
  TITLE     RNA targeting with CRISPR-Cas13.
  JOURNAL   Nature. 2017 Oct 4. doi: 10.1038/nature24049.
  PUBMED    28976959
REFERENCE   2  (bases 1 to 896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2017 Oct 4. doi: 10.1038/nature24049."
COMMENT     Sequence Label: pC016 - LwCas13a guide expression backbone wit...
FEATURES             Location/Qualifiers
     source          1..896
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        61..309
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     61..81
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     232..251
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     rep_origin      445..873
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(532..551)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     742..763
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcagctagc
       61 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
      121 ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
      181 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
      241 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
      301 cgaaacaccg atttagacta ccccaaaaac gaaggggact aaaacgggtc ttcgagaaga
      361 cctttttttt gaattctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
      421 cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg
      481 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cttagcgccc gctcctttcg
      541 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
      601 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
      661 tgggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
      721 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaactcta
      781 tctcgggcta ttcttttgat ttataaggga ttttgccgat ttcggtctat tggttaaaaa
      841 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttaca
//
