LOCUS       sequence-296412-        3597 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mouse gamma-synuclein (mSncg) promoter-mediates gene expression in 
            retinal ganglion cells with fluorescent reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3597)
  AUTHORS   Wang Q, Zhuang PS, Huang H, Li L, Liu L, Webber HC, Dalal R, Siew L,
            Fligor CM, Chang KC, Nahmou M, Kreymerman A, Sun Y, Meyer JS, 
            Goldberg JL, Hu Y
  TITLE     Mouse gamma-Synuclein Promoter-Mediated Gene Expression and Editing 
            in Mammalian Retinal Ganglion Cells.
  JOURNAL   J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0102-20.2020. doi: 
            10.1523/JNEUROSCI.0102-20.2020.
  PUBMED    32300046
REFERENCE   2  (bases 1 to 3597)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Neurosci.
            2020 Apr 9. pii: JNEUROSCI.0102-20.2020. doi: 
            10.1523/JNEUROSCI.0102-20.2020."
COMMENT     Sequence Label: pAM-AAV-mSncg-EGFP
FEATURES             Location/Qualifiers
     source          1..3597
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     repeat_region   1..130
                     /label=AAV2 ITR
     CDS             join(1760..1762,1763..1765,1766..2479)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1760..1762,1763..1765,1766..2476)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1805..1826)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2066..2085)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2413..2434
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2537..3125
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2590..2610)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3008..3019)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3152..3169)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3158..3382
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   3468..3597
                     /label=AAV2 ITR
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct tgtagttaat gattaacccg ccatgctact tatttacgta gccatgctct
      181 aggtaccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      241 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      301 atcatatggt cccatgccac tagtgggagc tgtgttacct gttgcagccc cacccaaagc
      361 ccctgctata ggtcaagcag gaatcaccct gccatcccca gcctggggcc tggagtacca
      421 gatccaggaa actagcatcc cttagctata gagatagcca cacatcagcc cattcctcag
      481 atgtgtatct ggggctcaga catcatctcc cgatctccga caagggcagg atttccttac
      541 cgtctgatgg ggtctctgct ggtatcctca gcccctagtc tccagccttc aggcatgccg
      601 ggcctattga gatgggagaa cttggtaccg gggtcctgtg cccaggaccc tagcagtccc
      661 cagctcaggt acaccccaaa gcccagcagc agtgtcggga tcatggtgag gggctcctgt
      721 gctgatgctc agccttacaa gtgactctca aatttgctgg tgatgtggtc ttcaagcgaa
      781 atgtcagaaa gaaaagaaaa cacgaggaca acaaagggag gaagtggcct ggtccggccc
      841 acccggcaag tctcatccgc ccccgccccc gccccttcca gcctggcccc cttggaggcc
      901 tccaaccact caggtcaatt cctgtgtcct gagggcactt gaatcaggga cacgggattt
      961 ggtagacaca taaaggtggc cccattaaac ttatttctcc aggactctgt cgtgggcctg
     1021 gaggagatct ggtgccaccc atactgttgg ccaggaagtg gggaacgggc acatcacacc
     1081 tgctcggcac cttgggctat gggaactagc aggtgggtgg gaactcagag aaggaaaggg
     1141 actatgctag aatcacacag cgggcagccc agtctagggc atggggagca gctttgggtg
     1201 tttctggcct cagccttcca acaggtttgg ctagagctcc aggctcaaga gcatccagga
     1261 tacagtgggg aactggataa cagggcagcc tgcaggttgg ccattcattg gattggccct
     1321 gaccccggcc cagcctggga cactgaggca tcatcagtca aggcactttt cttctgcata
     1381 taagagccag ggcacgagac caccagggct ttccaaggat gaatgaggtg taatgataga
     1441 ttaggatatg tccagcctcc aacacgctct ccctccccca gggccaacaa gagtcagcag
     1501 ggcagaatag agccagtagg ggcccgggcc ctgctcgctg gtatccccgt gaggcatgcc
     1561 ttctctctgg cccgccctcc ctgcccccac cctggcccgg gctggctggg ctccagccag
     1621 cagccacagc atcaatattt catctgcgtc aataagaggc agtagcagca gagacagcgg
     1681 ctgcggcagc actccagtcc atagcttgca gcagccaggt tccatccttg caaacaccat
     1741 ggacgtcttc aaggaattca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
     1801 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
     1861 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
     1921 gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg
     1981 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
     2041 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
     2101 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
     2161 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat
     2221 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga
     2281 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
     2341 gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga
     2401 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
     2461 ggacgagctg tacaagtaaa gatctacgcg tggtacctct agagtcgacc cgggcggcct
     2521 cgagaagctt atcgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     2581 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     2641 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     2701 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     2761 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     2821 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     2881 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc
     2941 ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     3001 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     3061 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     3121 cccgcatcga taccgtcgac tcgctgatca gcctcgactg tgccttctag ttgccagcca
     3181 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     3241 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     3301 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
     3361 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctcgactaga
     3421 gcatggctac gtagataagt agcatggcgg gttaatcatt aactacaagg aacccctagt
     3481 gatggagttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa
     3541 ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag cgcgcag
//
