LOCUS       sequence-296421-        7124 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Prey vector BAM3 X078_pECIA14 should be used with bait vector BAM3 
            X078_pECIA2..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7124)
  AUTHORS   Smakowska-Luzan E, Mott GA, Parys K, Stegmann M, Howton TC, 
            Layeghifard M, Neuhold J, Lehner A, Kong J, Grunwald K, Weinberger 
            N, Satbhai SB, Mayer D, Busch W, Madalinski M, Stolt-Bergner P, 
            Provart NJ, Mukhtar MS, Zipfel C, Desveaux D, Guttman DS, Belkhadir 
            Y
  TITLE     An extracellular network of Arabidopsis leucine-rich repeat receptor
            kinases.
  JOURNAL   Nature. 2018 Jan 18;553(7688):342-346. doi: 10.1038/nature25184. 
            Epub 2018 Jan 10.
  PUBMED    29320478
REFERENCE   2  (bases 1 to 7124)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature25184"; journalName: "Nature"; date: "2018-01-18- 
            18"; volume: "553"; issue: "7688"; pages: "342-346"
COMMENT     Sequence Label: BAM3 X078_pECIA14
FEATURES             Location/Qualifiers
     source          1..7124
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        23..449
                     /label=MT promoter
                     /note="Drosophila metallothionein promoter"
     sig_peptide     467..520
                     /codon_start=1
                     /product="Drosophila BiP signal sequence"
                     /label=BiP signal sequence
                     /translation="MKLCILLAVVAFVGLSLG"
     CDS             2420..2443
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             4118..4141
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4157..4174
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4197..4214)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4247..4381
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4271..4290)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4325..4344
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(4525..4541)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4525..4541)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4538..4560)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4549..4565
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4573..4579,4580..4597,4598..4603))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4618..4639
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4756..4773)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4927..5515)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5007..5026)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5686..6477,6478..6546))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6309..6328
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6547..6651)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6719..6737
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6775..6797)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6897..6916
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     join(7110..7124,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 gtaaaacgac ggccagtgaa ttaattcgtt gcaggacagg atgtggtgcc cgatgtgact
       61 agctctttgc tgcaggccgt cctatcctct ggttccgata agagacccag aactccggcc
      121 ccccaccgcc caccgccacc cccatacata tgtggtacgc aagtaagagt gcctgcgcat
      181 gccccatgtg ccccaccaag agctttgcat cccatacaag tccccaaagt ggagaaccga
      241 accaattctt cgcgggcaga acaaaagctt ctgcacacgt ctccactcga atttggagcc
      301 ggccggcgtg tgcaaaagag gtgaatcgaa cgaaagaccc gtgtgtaaag ccgcgtttcc
      361 aaaatgtata aaaccgagag catctggcca atgtgcatca gttgtggtca gcagcaaaat
      421 caagtgaatc atctcagtgc aactaaaggg gggatccgat ctcaatatga agttatgcat
      481 attactggcc gtcgtggcct ttgttggcct ctcgctcggg tctttgatct cacctcttaa
      541 tctctcactt attagacaag caaatgtcct tatctctcta aagcaaagtt ttgattccta
      601 tgatccttct cttgattcat ggaacattcc aaatttcaac tctctatgtt cttggactgg
      661 tgtttcttgt gacaacttga atcagtctat tactcgtcta gacctatcta atctcaacat
      721 ctccggcact atctctccgg aaatatctcg tctttcgccg tcacttgttt ttcttgacat
      781 ttcttctaac agtttctccg gtgagcttcc taaagagatc tatgagctct caggcctcga
      841 agtgttaaac atctctagca atgtttttga aggagagctg gagacacgtg ggttcagtca
      901 aatgactcag cttgtgactc ttgacgctta cgacaacagc ttcaacggat cacttcctct
      961 gagtctaacc acactcactc gtctcgagca cttagatctt ggaggaaact acttcgacgg
     1021 tgagatccct agaagctatg gaagtttctt gagtctcaag tttctttctt tatctggtaa
     1081 tgatctccgt gggagaatcc ctaacgagct agcgaacatc acgactttgg tacagcttta
     1141 cttaggttac tacaacgatt accgcggtgg gatacctgca gatttcggga gattgatcaa
     1201 tcttgttcat ttggatttag ctaattgcag cttgaaagga tcaattcctg cagaattggg
     1261 gaatctcaag aacttggagg ttctgtttct tcagaccaat gagcttacag gctctgttcc
     1321 tcgagagtta gggaacatga caagcctcaa gactcttgat ctctccaaca actttcttga
     1381 aggagagatt cctctagagc tatctggact tcaaaagctt cagttgttta acctcttctt
     1441 caacagacta cacggcgaga tccctgagtt cgtatctgag cttcctgatc tgcaaatact
     1501 caagctttgg cacaacaatt tcaccggaaa gattccttcg aaactcggat caaacgggaa
     1561 cttgatcgag atcgatttgt ctaccaataa actcacaggt ttgatccctg agtcactctg
     1621 tttcggaaga agactaaaga ttctcattct cttcaacaac ttcttgttcg gtcctctccc
     1681 tgaagatctt ggccaatgtg aaccgctatg gagattccgt ctcggacaga actttctgac
     1741 aagtaagttg ccaaagggtt tgatttattt gccgaatctt tcgcttcttg agcttcaaaa
     1801 caactttttg actggagaaa tccccgaaga agaggcggga aatgcgcagt tttcgagcct
     1861 tactcagatc aatctgtcca acaacaggtt atccggaccg attcctggtt caatcagaaa
     1921 cctcagaagc cttcagattc ttcttctcgg tgcaaaccgg ttatcgggac agatccctgg
     1981 cgaaatcgga agtttgaaga gtcttctcaa gattgacatg agcagaaaca acttctcagg
     2041 caagtttcct cctgagtttg gtgattgcat gtcactcaca tatttagatt tgagtcacaa
     2101 ccagatttcc ggtcagattc cggttcagat atcgcagatt cggattctaa actatctgaa
     2161 tgtttcttgg aattccttta accaaagcct tcccaacgaa ctcggataca tgaagagttt
     2221 aacatcagca gatttctcac acaacaactt ctccggttca gtaccaactt cagggcaatt
     2281 ctcttacttc aacaacacgt cattccttgg aaaccctttt ctctgtggat tttcttcaaa
     2341 cccttgcaac ggttcccaaa accaatctca atctcagcta cttaaccaga acaacgcaag
     2401 atcccgaggt gaaatctccc tggaggtgct gttccaggga ccaagcggca atcccggcgg
     2461 cgatctggcc ccccagatgc tgcgcgagct gcaggagacc aatgccgccc tgcaggacgt
     2521 gcgcgagctg ctgcgccacc gcgtgaagga gatcaccttc ctgaagaaca ccgtgatgga
     2581 gtgcgacgcc tgcggcatgc agccctctag aggctccggc tccatcatcc cagttgagga
     2641 ggagaacccg gacttctgga accgcgaggc agccgaggcc ctgggtgccg ccaagaagct
     2701 gcagcctgca cagacagccg ccaagaacct catcatcttc ctgggcgatg ggatgggggt
     2761 gtctacggtg acagctgcca ggatcctaaa agggcagaag aaggacaaac tggggcctga
     2821 gatacccctg gccatggacc gcttcccata tgtggctctg tccaagacat acaatgtaga
     2881 caaacatgtg ccagacagtg gagccacagc cacggcctac ctgtgcgggg tcaagggcaa
     2941 cttccagacc attggcttga gtgcagccgc ccgctttaac cagtgcaaca cgacacgcgg
     3001 caacgaggtc atctccgtga tgaatcgggc caagaaagca gggaagtcag tgggagtggt
     3061 aaccaccaca cgagtgcagc acgcctcgcc agccggcacc tacgcccaca cggtgaaccg
     3121 caactggtac tcggacgccg acgtgcctgc ctcggcccgc caggaggggt gccaggacat
     3181 cgctacgcag ctcatctcca acatggacat tgacgtgatc ctaggtggag gccgaaagta
     3241 catgtttcgc atgggaaccc cagaccctga gtacccagat gactacagcc aaggtgggac
     3301 caggctggac gggaagaatc tggtgcagga atggctggcg aagcgccagg gtgcccggta
     3361 tgtgtggaac cgcactgagc tcatgcaggc ttccctggac ccgtctgtga cccatctcat
     3421 gggtctcttt gagcctggag acatgaaata cgagatccac cgagactcca cactggaccc
     3481 ctccctgatg gagatgacag aggctgccct gcgcctgctg agcaggaacc cccgcggctt
     3541 cttcctcttc gtggagggtg gtcgcatcga ccatggtcat catgaaagca gggcttaccg
     3601 ggcactgact gagacgatca tgttcgacga cgccattgag agggcgggcc agctcaccag
     3661 cgaggaggac acgctgagcc tcgtcactgc cgaccactcc cacgtcttct ccttcggagg
     3721 ctaccccctg cgagggagct ccatcttcgg gctggcccct ggcaaggccc gggacaggaa
     3781 ggcctacacg gtcctcctat acggaaacgg tccaggctat gtgctcaagg acggcgcccg
     3841 gccggatgtt accgagagcg agagcgggag ccccgagtat cggcagcagt cagcagtgcc
     3901 cctggacgaa gagacccacg caggcgagga cgtggcggtg ttcgcgcgcg gcccgcaggc
     3961 gcacctggtt cacggcgtgc aggagcagac cttcatagcg cacgtcatgg ccttcgccgc
     4021 ctgcctggag ccctacaccg cctgcgacct ggcgcccccc gccggcacca ccgacgccgc
     4081 gcacccgggt tctagaggaa gcacctccgg cgataacgat tacaaggatg atgatgataa
     4141 ggccagcggc accggtcatc atcaccatca ccattgagtt taaacccgct gatcagcctc
     4201 gactgtgcct tctaaggcct gagctcgctg atcagcctcg atcgaggatc cagacatgat
     4261 aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat
     4321 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt
     4381 taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt
     4441 ttaaagcaag taaaacctct acaaatgtgg tatggctgat tatgatcagt cgacctgcag
     4501 gcatgcaagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     4561 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     4621 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     4681 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     4741 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     4801 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     4861 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     4921 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     4981 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     5041 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     5101 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     5161 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     5221 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     5281 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     5341 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     5401 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     5461 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     5521 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     5581 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     5641 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     5701 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     5761 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     5821 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     5881 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     5941 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     6001 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     6061 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     6121 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     6181 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     6241 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     6301 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     6361 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     6421 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     6481 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     6541 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     6601 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     6661 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
     6721 taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg
     6781 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
     6841 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc
     6901 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt
     6961 aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg
     7021 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag
     7081 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgtt
//
