LOCUS       sequence-296433-        9208 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector, with insert of NSP3 gene's CDS from
            SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9208)
  AUTHORS   Kim DK, Knapp JJ, Kuang D, Chawla A, Cassonnet P, Lee H, 
            Sheykhkarimli D, Samavarchi-Tehrani P, Abdouni H, Rayhan A, Li R, 
            Pogoutse O, Coyaud E, van der Werf S, Demeret C, Gingras AC, Taipale
            M, Raught B, Jacob Y, Roth FP
  TITLE     A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions.
  JOURNAL   G3 (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554.
  PUBMED    32763951
REFERENCE   2  (bases 1 to 9208)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554."
COMMENT     Sequence Label: pDONR207 SARS-CoV-2 NSP3_nostop
FEATURES             Location/Qualifiers
     source          1..9208
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(63..90)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(182..268)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    321..420
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(6259..6358)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(6470..6489)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(6548..6567)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     CDS             complement(7141..7674)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(7863..7868,7869..7885,7886..7891))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     rep_origin      8548..9136
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9037..9056
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttatcccctg attctgtgga taaccgtatt accgctagcc aggaagagtt tgtagaaacg
       61 caaaaaggcc atccgtcagg atggccttct gcttagtttg atgcctggca gtttatggcg
      121 ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc gctcccggcg
      181 gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt
      241 cttccgactg agcctttcgt tttatttgat gcctggcagt tccctactct cgcgttaacg
      301 ctagcatgga tctcgggccc caaataatga ttttattttg actgatagtg acctgttcgt
      361 tgcaacaaat tgatgagcaa tgctttttta taatgccaac tttgtacaaa aaagttggca
      421 tggcccctac caaggtgacc ttcggagacg acaccgtgat cgaggtgcag ggttacaaga
      481 gcgtgaacat caccttcgag ctggacgaac gcatcgacaa ggtgctgaac gaaaagtgct
      541 ccgcttacac cgtggagctg ggcaccgagg tgaacgaatt cgcctgcgtg gtggccgacg
      601 ctgtgatcaa gaccctgcag ccagtgtccg aactgctgac ccctctgggt atcgacctgg
      661 acgagtggtc tatggctacc tactacctgt tcgacgagag cggcgaattc aagctggcct
      721 ctcacatgta ctgcagcttc taccctcccg acgaagacga ggaagagggt gactgcgaag
      781 aggaagagtt cgagccttcc actcagtacg agtacggcac cgaagacgac taccagggca
      841 agcctctgga attcggagcc acctctgccg ctctgcagcc tgaagaggaa caggaggaag
      901 actggctgga cgacgactcc cagcagaccg tgggtcagca ggacggctct gaagacaacc
      961 agaccaccac catccagacc atcgtggagg tgcagcctca gctggagatg gaactgaccc
     1021 cagtggtgca gaccatcgag gtgaacagct tctccggcta cctgaagctg accgacaacg
     1081 tgtacatcaa gaacgctgac atcgtggagg aagccaagaa ggtgaaacca accgtggtgg
     1141 tgaacgccgc taacgtgtac ctgaagcacg gcggaggtgt ggctggagct ctgaacaagg
     1201 ctaccaacaa cgccatgcag gtggaatccg acgactacat cgctaccaac ggtcctctga
     1261 aggtgggcgg atcctgcgtg ctgtctggcc acaacctggc caagcactgc ctgcacgtgg
     1321 tgggaccaaa cgtgaacaag ggagaggaca tccagctgct gaagagcgcc tacgaaaact
     1381 tcaaccagca cgaggtgctg ctggctccac tgctgtctgc cggaatcttc ggtgctgatc
     1441 ccatccacag cctgcgcgtg tgcgtggaca ccgtgaggac caacgtgtac ctggccgtgt
     1501 tcgacaagaa cctgtacgac aagctggtga gctccttcct ggagatgaag tccgaaaagc
     1561 aggtggagca gaagatcgcc gagatcccca aggaggaagt gaagcctttc atcaccgaat
     1621 ctaagcccag cgtggagcag agaaagcagg acgacaagaa gatcaaggcc tgcgtggagg
     1681 aagtgaccac caccctggag gaaaccaagt tcctgaccga gaacctgctg ctgtacatcg
     1741 acatcaacgg caacctgcac cctgactccg ctaccctggt gtctgacatc gacatcacct
     1801 tcctgaagaa ggacgccccc tacatcgtgg gcgacgtggt gcaggaagga gtgctgaccg
     1861 ctgtggtcat ccctaccaag aaggccggtg gcaccaccga gatgctggcc aaggctctgc
     1921 gtaaggtgcc aaccgacaac tacatcacca cctaccccgg tcagggcctg aacggttaca
     1981 ccgtggagga agctaagacc gtgctgaaga agtgcaagtc tgccttctac atcctgccct
     2041 ccatcatctc taacgagaag caggaaatcc tgggcaccgt gagctggaac ctgcgcgaaa
     2101 tgctggccca cgctgaggaa accaggaagc tgatgcccgt gtgcgtggag accaaggcca
     2161 tcgtgagcac catccagaga aagtacaagg gcatcaagat ccaggagggt gtggtggact
     2221 acggcgctcg tttctacttc tacacctcca agaccaccgt ggcctctctg atcaacaccc
     2281 tgaacgacct gaacgagacc ctggtgacca tgccactggg atacgtgacc cacggtctga
     2341 acctggagga agccgctaga tacatgcgtt ccctgaaggt gcctgctacc gtgagcgtga
     2401 gcagccctga cgctgtgacc gcctacaacg gatacctgac ctcctctagc aagacccctg
     2461 aggaacactt catcgaaacc atcagcctgg ccggttccta caaggactgg tcttacagcg
     2521 gacagtccac ccagctgggc atcgagttcc tgaagcgcgg agacaagagc gtgtactaca
     2581 cctccaaccc aaccaccttc cacctggacg gtgaagtgat caccttcgac aacctgaaga
     2641 ccctgctgtc tctgcgcgag gtgaggacca tcaaggtgtt caccaccgtg gacaacatca
     2701 acctgcacac ccaggtggtg gacatgagca tgacctacgg acagcagttc ggtcctacct
     2761 acctggacgg agctgacgtg accaagatca agccacacaa ctcccacgag ggcaagacct
     2821 tctacgtgct gcctaacgat gacaccctga gagtggaggc tttcgaatac taccacacca
     2881 ccgacccatc cttcctgggc aggtacatgt ctgccctgaa ccacaccaag aagtggaagt
     2941 acccccaggt gaacggactg accagcatca agtgggccga caacaactgc tacctggcca
     3001 ccgctctgct gaccctgcag cagatcgaac tgaagttcaa cccacccgct ctgcaggacg
     3061 cttactacag agcccgtgct ggcgaggccg ctaacttctg cgctctgatc ctggcctact
     3121 gcaacaagac cgtgggcgaa ctgggagacg tgagagagac catgagctac ctgttccagc
     3181 acgccaacct ggactcctgc aagcgtgtgc tgaacgtggt gtgcaagacc tgcggccagc
     3241 agcagaccac cctgaaggga gtggaagctg tgatgtacat gggtaccctg tcctacgagc
     3301 agttcaagaa gggcgtgcag atcccttgca cctgcggaaa gcaggccacc aagtacctgg
     3361 tgcagcagga aagcccattc gtgatgatgt ccgctcctcc agcccagtac gaactgaagc
     3421 acggcacctt cacctgcgct tccgagtaca ccggcaacta ccagtgcgga cactacaagc
     3481 acatcacctc taaggagacc ctgtactgca tcgacggagc cctgctgacc aagtcctctg
     3541 aatacaaggg tcccatcacc gacgtgttct acaaggagaa cagctacacc accaccatca
     3601 agcccgtgac ctacaagctg gacggcgtgg tgtgcaccga aatcgaccct aagctggaca
     3661 actactacaa gaaggacaac tcttacttca ccgagcagcc tatcgacctg gtgccaaacc
     3721 agccctaccc taacgccagc ttcgacaact tcaagttcgt gtgcgacaac atcaagttcg
     3781 ctgacgacct gaaccagctg accggataca agaagcctgc ctctagagag ctgaaggtga
     3841 ccttcttccc agacctgaac ggcgacgtgg tggctatcga ctacaagcac tacaccccaa
     3901 gcttcaagaa gggagccaag ctgctgcaca agcccatcgt gtggcacgtg aacaacgcta
     3961 ccaacaaggc cacctacaag cctaacacct ggtgcatccg ttgcctgtgg tctaccaagc
     4021 cagtggaaac ctccaactct ttcgacgtgc tgaagagcga ggacgctcag ggcatggaca
     4081 acctggcctg cgaagacctg aagcccgtga gcgaggaagt ggtggagaac ccaaccatcc
     4141 agaaggacgt gctggaatgc aacgtgaaga ccaccgaggt ggtgggagac atcatcctga
     4201 agccagctaa caacagcctg aagatcaccg aggaagtggg tcacaccgac ctgatggccg
     4261 cttacgtgga caacagctcc ctgaccatca agaagcccaa cgagctgagc agggtgctgg
     4321 gtctgaagac cctggctact cacggtctgg ctgctgtgaa ctctgtgcca tgggacacca
     4381 tcgctaacta cgccaagcct ttcctgaaca aggtggtgtc caccaccacc aacatcgtga
     4441 ccagatgtct gaaccgcgtg tgcaccaact acatgcccta cttcttcacc ctgctgctgc
     4501 agctgtgcac cttcaccaga agcaccaact cccgtatcaa ggctagcatg cctaccacca
     4561 tcgccaagaa caccgtgaag agcgtgggaa agttctgcct ggaggcttcc ttcaactacc
     4621 tgaagtctcc aaacttcagc aagctgatca acatcatcat ctggttcctg ctgctctccg
     4681 tgtgcctggg ttccctgatc tactctaccg ccgctctggg agtgctgatg tctaacctgg
     4741 gtatgcctag ctactgcact ggttacagag agggatacct gaacagcacc aacgtgacca
     4801 tcgccaccta ctgcaccggc agcatcccat gctccgtgtg cctgtctgga ctggacagcc
     4861 tggacaccta cccctccctg gaaaccatcc agatcaccat ctctagcttc aagtgggacc
     4921 tgaccgcctt cggtctggtg gctgagtggt tcctggccta catcctgttc accaggttct
     4981 tctacgtgct gggcctggcc gctatcatgc agctgttctt ctcctacttc gctgtgcact
     5041 tcatcagcaa ctcctggctg atgtggctga tcatcaacct ggtgcagatg gctcccatct
     5101 ctgccatggt gcgcatgtac atcttcttcg ccagcttcta ctacgtgtgg aagtcctacg
     5161 tgcacgtggt ggacggttgc aactcctcta cctgcatgat gtgctacaag cgcaacaggg
     5221 ctaccagggt ggagtgcacc accatcgtga acggcgtgcg caggtccttc tacgtgtacg
     5281 ccaacggcgg caagggattc tgcaagctgc acaactggaa ctgcgtgaac tgcgacacct
     5341 tctgcgctgg ctccaccttc atctctgacg aggtggccag agacctgagc ctgcagttca
     5401 agcgtccaat caaccccacc gaccagagct cctacatcgt ggactctgtg accgtgaaga
     5461 acggtagcat ccacctgtac ttcgacaagg ctggccagaa gacctacgag cgccactctc
     5521 tgagccactt cgtgaacctg gacaacctga gggccaacaa caccaagggc agcctgccta
     5581 tcaacgtgat cgtgttcgac ggaaagtcca agtgcgagga atctagcgcc aagtccgcca
     5641 gcgtgtacta cagccagctg atgtgccagc caatcctgct gctggaccag gctctggtgt
     5701 ctgacgtggg agacagcgcc gaagtggccg tgaagatgtt cgacgcctac gtgaacacct
     5761 tctcctctac cttcaacgtg cctatggaga agctgaagac cctggtggct actgctgagg
     5821 ctgaactggc taagaacgtg tccctggaca acgtgctgag caccttcatc tccgccgcta
     5881 ggcagggttt cgtggactct gacgtggaaa ccaaagacgt ggtggagtgc ctgaagctgt
     5941 ctcaccagag cgacatcgaa gtgaccggcg actcctgcaa caactacatg ctgacctaca
     6001 acaaggtgga gaacatgacc ccaagagacc tgggagcttg catcgactgc tccgcccgtc
     6061 acatcaacgc tcaggtggcc aagtctcaca acatcgccct gatctggaac gtgaaggact
     6121 tcatgagcct gtccgagcag ctgcgcaagc agatcaggag cgccgctaag aagaacaacc
     6181 tgcccttcaa gctgacctgc gctaccacca gacaggtggt gaacgtggtg accaccaaga
     6241 tcgccctgaa gggcggatac ccaactttct tgtacaaagt tggcattata agaaagcatt
     6301 gcttatcaat ttgttgcaac gaacaggtca ctatcagtca aaataaaatc attatttgcc
     6361 atccagctgc agctctggcc cgtgtctcaa aatctctgat gttacattgc acaagataaa
     6421 aatatatcat catgaacaat aaaactgtct gcttacataa acagtaatac aaggggtgtt
     6481 atgagccata ttcaacggga aacgtcgagg ccgcgattaa attccaacat ggatgctgat
     6541 ttatatgggt ataaatgggc tcgcgataat gtcgggcaat caggtgcgac aatctatcgc
     6601 ttgtatggga agcccgatgc gccagagttg tttctgaaac atggcaaagg tagcgttgcc
     6661 aatgatgtta cagatgagat ggtcagacta aactggctga cggaatttat gcctcttccg
     6721 accatcaagc attttatccg tactcctgat gatgcatggt tactcaccac tgcgatcccc
     6781 ggaaaaacag cattccaggt attagaagaa tatcctgatt caggtgaaaa tattgttgat
     6841 gcgctggcag tgttcctgcg ccggttgcat tcgattcctg tttgtaattg tccttttaac
     6901 agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa tgaataacgg tttggttgat
     6961 gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg
     7021 cataaacttt tgccattctc accggattca gtcgtcactc atggtgattt ctcacttgat
     7081 aaccttattt ttgacgaggg gaaattaggc cgggaagccg atctcggctt gaacgaattg
     7141 ttaggtggcg gtacttgggt cgatatcaaa gtgcatcact tcttcccgta tgcccaactt
     7201 tgtatagaga gccactgcgg gatcgtcacc gtaatctgct tgcacgtaga tcacataagc
     7261 accaagcgcg ttggcctcat gcttgaggag attgatgagc gcggtggcaa tgccctgcct
     7321 ccggtgctcg ccggagactg cgagatcata gatatagatc tcactacgcg gctgctcaaa
     7381 cttgggcaga acgtaagccg cgagagcgcc aacaaccgct tcttggtcga aggcagcaag
     7441 cgcgatgaat gtcttactac ggagcaagtt cccgaggtaa tcggagtccg gctgatgttg
     7501 ggagtaggtg gctacgtctc cgaactcacg accgaaaaga tcaagagcag cccgcatgga
     7561 tttgacttgg tcagggccga gcctacatgt gcgaatgatg cccatacttg agccacctaa
     7621 ctttgtttta gggcgactgc cctgctgcgt aacatcgttg ctgctgcgta acatcgttgc
     7681 tgctccataa catcaaacat cgacccacgg cgtaacgcgc ttgctgcttg gatgcccgag
     7741 gcatagactg tacaaaaaaa cagtcataac aagccatgaa aaccgccact gcgccgttac
     7801 caccgctgcg ttcggtcaag gttctggacc agttgcgtga gcgcatacgc tacttgcatt
     7861 acagtttacg aaccgaacag gcttatgtca actgggttcg tgccttcatc cgtttccacg
     7921 gtgtgcgtca cccggcaacc ttgggcagca gcgaagtcga ggcatttctg tcctggctgg
     7981 cgaacgagcg caaggtttcg gtctccacgc atcgtcaggc attggcggcc ttgctgttct
     8041 tctacggcaa ggtgctgtgc acggatctgc cctggcttca ggagatcgga agacctcggc
     8101 cgtcgcggcg cttgccggtg gtgctgaccc cggatgaagt ggttcgcatc ctcggttttc
     8161 tggaaggcga gcatcgtttg ttcgcccagg actctagcta tagttctagt ggttggctac
     8221 taataggttg tattgatgtt ggacgagtcg gaatcgcaga ccgataccag gatcttgcca
     8281 tcctatggaa ctgcctcggt gagttttctc cttcattaca gaaacggctt tttcaaaaat
     8341 atggtattga taatcctgat atgaataaat tgcagtttca tttgatgctc gatgagtttt
     8401 tctaatcaga attggttaat tggttgtaac actggcagag cattacgctg acttgacggg
     8461 acggcgcaag ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     8521 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     8581 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     8641 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     8701 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     8761 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     8821 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     8881 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     8941 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     9001 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     9061 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     9121 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     9181 gccttttgct cacatgttct ttcctgcg
//
