LOCUS       sequence-296547-        3936 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mouse gamma-synuclein (mSncg) promoter-mediates Cre expression in 
            retinal ganglion cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3936)
  AUTHORS   Wang Q, Zhuang PS, Huang H, Li L, Liu L, Webber HC, Dalal R, Siew L,
            Fligor CM, Chang KC, Nahmou M, Kreymerman A, Sun Y, Meyer JS, 
            Goldberg JL, Hu Y
  TITLE     Mouse gamma-Synuclein Promoter-Mediated Gene Expression and Editing 
            in Mammalian Retinal Ganglion Cells.
  JOURNAL   J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0102-20.2020. doi: 
            10.1523/JNEUROSCI.0102-20.2020.
  PUBMED    32300046
REFERENCE   2  (bases 1 to 3936)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Neurosci.
            2020 Apr 9. pii: JNEUROSCI.0102-20.2020. doi: 
            10.1523/JNEUROSCI.0102-20.2020."
COMMENT     Sequence Label: pAM-AAV-mSncg-Cre
FEATURES             Location/Qualifiers
     source          1..3936
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     repeat_region   1..130
                     /label=AAV2 ITR
     CDS             1769..1789
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1790..2818
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="SNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKMLL
                     SVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGLP
                     RPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAFL
                     GIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVER
                     WISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQR
                     YLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLED
                     GD"
     misc_feature    2876..3464
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2929..2949)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3347..3358)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3491..3508)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3497..3721
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   3807..3936
                     /label=AAV2 ITR
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct tgtagttaat gattaacccg ccatgctact tatttacgta gccatgctct
      181 aggtaccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      241 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      301 atcatatggt cccatgccac tagtgggagc tgtgttacct gttgcagccc cacccaaagc
      361 ccctgctata ggtcaagcag gaatcaccct gccatcccca gcctggggcc tggagtacca
      421 gatccaggaa actagcatcc cttagctata gagatagcca cacatcagcc cattcctcag
      481 atgtgtatct ggggctcaga catcatctcc cgatctccga caagggcagg atttccttac
      541 cgtctgatgg ggtctctgct ggtatcctca gcccctagtc tccagccttc aggcatgccg
      601 ggcctattga gatgggagaa cttggtaccg gggtcctgtg cccaggaccc tagcagtccc
      661 cagctcaggt acaccccaaa gcccagcagc agtgtcggga tcatggtgag gggctcctgt
      721 gctgatgctc agccttacaa gtgactctca aatttgctgg tgatgtggtc ttcaagcgaa
      781 atgtcagaaa gaaaagaaaa cacgaggaca acaaagggag gaagtggcct ggtccggccc
      841 acccggcaag tctcatccgc ccccgccccc gccccttcca gcctggcccc cttggaggcc
      901 tccaaccact caggtcaatt cctgtgtcct gagggcactt gaatcaggga cacgggattt
      961 ggtagacaca taaaggtggc cccattaaac ttatttctcc aggactctgt cgtgggcctg
     1021 gaggagatct ggtgccaccc atactgttgg ccaggaagtg gggaacgggc acatcacacc
     1081 tgctcggcac cttgggctat gggaactagc aggtgggtgg gaactcagag aaggaaaggg
     1141 actatgctag aatcacacag cgggcagccc agtctagggc atggggagca gctttgggtg
     1201 tttctggcct cagccttcca acaggtttgg ctagagctcc aggctcaaga gcatccagga
     1261 tacagtgggg aactggataa cagggcagcc tgcaggttgg ccattcattg gattggccct
     1321 gaccccggcc cagcctggga cactgaggca tcatcagtca aggcactttt cttctgcata
     1381 taagagccag ggcacgagac caccagggct ttccaaggat gaatgaggtg taatgataga
     1441 ttaggatatg tccagcctcc aacacgctct ccctccccca gggccaacaa gagtcagcag
     1501 ggcagaatag agccagtagg ggcccgggcc ctgctcgctg gtatccccgt gaggcatgcc
     1561 ttctctctgg cccgccctcc ctgcccccac cctggcccgg gctggctggg ctccagccag
     1621 cagccacagc atcaatattt catctgcgtc aataagaggc agtagcagca gagacagcgg
     1681 ctgcggcagc actccagtcc atagcttgca gcagccaggt tccatccttg caaacaccat
     1741 ggacgtcttc aaggaattcg ccaccatgcc caagaagaag aggaaggtgt ccaatttact
     1801 gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa
     1861 gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct
     1921 tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc
     1981 cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt
     2041 aaaaactatc cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc
     2101 acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt
     2161 tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt
     2221 tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg
     2281 gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat
     2341 ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag
     2401 caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat
     2461 ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa
     2521 tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt
     2581 tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc
     2641 ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc
     2701 aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtca tgaactatat
     2761 ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattagag
     2821 atctacgcgt ggtacctcta gagtcgaccc gggcggcctc gagaagctta tcgataatca
     2881 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     2941 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     3001 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     3061 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     3121 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     3181 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     3241 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg
     3301 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     3361 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     3421 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat accgtcgact
     3481 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     3541 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     3601 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
     3661 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
     3721 gcttctgagg cggaaagaac cagctggggc tcgactagag catggctacg tagataagta
     3781 gcatggcggg ttaatcatta actacaagga acccctagtg atggagttgg ccactccctc
     3841 tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt
     3901 tgcccgggcg gcctcagtga gcgagcgagc gcgcag
//
